Difference between revisions of "Team:LZU-China/chz safety"

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<h5> We have taken safety courses before so the entire group members know how to protect ourselves in experiments.  
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<p>Our team determined to make all aspects of the entire project perfect, especially biosafety. The one and the only organisms we used is Escherichia coli whose lab strains that are not harmful to human at all. The main risks in our experiment is the heavy metal we used to exam our parts. To reduce that, we do many experiments in draught cupboards and we use latex gloves and masks to protect ourselves. </p>
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        As for environment, some of the masses hesitate it may cause genetic pollution to the environment, but it should be noticed that the gene modified E.coli we construct cannot express our target gene unless it is stimulated by the pollutant PNP.</h5>
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<br/>
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<p>Our device is designed of a safer future. We design a MFC-based device. The bacteria can sense many kinds of ions and it can improve the voltage in MFC. We modify the E.coli to make it can produce riboflavin when sensed ions. Our device is designed as an enclosed systems. The measurement of the wastewater is safe, and neither the genetically modified sensor cells nor the wastewater is impossible to leak at all. </p>
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<br/>
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<p>Our device can be very helpful in many fields. For more information, please contacted with Xiangkai Li </p>
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<br/>
 +
<br/>
 +
<br/>
 
<table class="responsive-table striped">
 
<table class="responsive-table striped">
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<thead>
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          <tbody>
 
          <tr>
 
          <tr>
−
            <th>&nbsp;</th>
+
            <th>Part number</th>
−
            <th>Species</th>
+
            <th>Part number</th>
−
            <th>Strain </th>
+
            <th>Where did us get the physical DNA for this part </th>
−
            <th>Risk Group</th>
+
            <th>What is the Risk Group of the species?</th>
−
            <th>Disease    risk to humans? If so, which disease</th>
+
            <th>How will you use it?</th>
 +
          </tr>
 +
        <tr>
 +
            <th>1</th>
 +
            <th>pSB1C3</th>
 +
            <th>From Li Xiangkai's Lab, Lanzhou University </th>
 +
            <th>1</th>
 +
            <th>Vector for E.coli to express BBA_K1755301
 +
Submission vector for all parts</th>
 +
          </tr>
 +
            <tr>
 +
            <th>2</th>
 +
            <th>K1755001</th>
 +
            <th>Synthetize</th>
 +
            <th>1</th>
 +
            <th>Every parts' foundation</th>
 +
          </tr>
 +
          <tr>
 +
            <th>3</th>
 +
            <th>K1755002</th>
 +
            <th>Parts construction</th>
 +
            <th>1</th>
 +
            <th>Foundation of other parts</th>
 +
          </tr>
 +
          <tr>
 +
            <th>4</th>
 +
            <th>K1755003</th>
 +
            <th>Parts construction</th>
 +
            <th>1</th>
 +
            <th>Foundation of other parts</th>
 +
          </tr>
 +
          <tr>
 +
            <th>5</th>
 +
            <th>K1755004</th>
 +
            <th>Parts construction</th>
 +
            <th>1</th>
 +
            <th>Testing riboflavin expression quantity</th>
 
          </tr>
 
          </tr>
−
        </thead>
 
−
          <tbody>
 
 
          <tr>
 
          <tr>
−
            <td>&nbsp;1</td>
+
            <th>6</th>
−
            <td><em>E. coli</em>K 12</td>
+
            <th>K1755005</th>
−
            <td>DH5 alpha</td>
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            <th>Parts construction</th>
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            <td><a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=&amp;species=coli">1</a></td>
+
            <th>1</th>
−
            <td>Yes. May    cause irritation to skin, eyes, and respiratory tract, may affect kidneys.    However, E. coli K-12 is not considered a human or animal pathogen nor is it    toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated    by its poor ability to colonize the colon and establish infections.</td>
+
            <th>Testing riboflavin expression quantity</th>
 +
          <tr>
 +
          <th>7</th>
 +
            <th>K1755006</th>
 +
            <th>Parts construction</th>
 +
            <th>1</th>
 +
            <th>Testing riboflavin expression quantity</th>
 +
          </tr>
 +
          <tr>
 +
          <th>8</th>
 +
            <th>K1755007</th>
 +
            <th>Parts construction</th>
 +
            <th>1</th>
 +
            <th>Testing riboflavin expression quantity</th>
 +
          </tr>
 +
          <tr>
 +
          <th>9</th>
 +
            <th>K1755008</th>
 +
            <th>Parts construction</th>
 +
            <th>1</th>
 +
            <th>Testing riboflavin expression quantity</th>
 +
          </tr>
 +
          <tr>
 +
          <th>10</th>
 +
            <th>K1755009</th>
 +
            <th>Parts construction</th>
 +
            <th>1</th>
 +
            <th>Testing riboflavin expression quantity</th>
 +
          </tr>
 +
          <tr>
 +
          <th>11</th>
 +
            <th>K1755301</th>
 +
            <th>Synthetize</th>
 +
            <th>1</th>
 +
            <th>Used in MFC</th>
 
          </tr>
 
          </tr>
 
          <tr>
 
          <tr>
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            <td>&nbsp;2</td>
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          <th>12</th>
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            <td><em>Shewenella    Onedensis </em></td>
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            <th>K1755302</th>
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            <td>MR1</td>
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            <th>Synthetize</th>
−
            <td><a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=&amp;species=coli">1</a></td>
+
            <th>1</th>
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            <td>No, S. oneidensis MR-1 belongs to a class of bacteria known as "Dissimilatory Metal-Reducing Bacteria (DMRB)" because of their ability to couple metal reduction with their metabolism. It cannot cause diseases</td>
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            <th>Used in MFC</th>
 
          </tr>
 
          </tr>
 +
          <tr>
 +
          <th>13</th>
 +
            <th>K1755303</th>
 +
            <th>Synthetize</th>
 +
            <th>1</th>
 +
            <th>Used in MFC</th>
 +
          </tr>
 +
          <tr>
 +
          <th>14</th>
 +
            <th>K1755305</th>
 +
            <th>Synthetize</th>
 +
            <th>1</th>
 +
            <th>Used in MFC</th>
 +
          </tr>
 +
          <tr>
 +
          <th>15</th>
 +
            <th>K1755024</th>
 +
            <th>Parts construction</th>
 +
            <th>1</th>
 +
            <th>Used in MFC</th>
 +
          </tr>
 +
 
        </tbody>
 
        </tbody>
 
</table>
 
</table>

Revision as of 21:44, 9 September 2015

Team:LZU 2015

 

Safety

(subtitle)this is our principle

 

 

Our team determined to make all aspects of the entire project perfect, especially biosafety. The one and the only organisms we used is Escherichia coli whose lab strains that are not harmful to human at all. The main risks in our experiment is the heavy metal we used to exam our parts. To reduce that, we do many experiments in draught cupboards and we use latex gloves and masks to protect ourselves.


Our device is designed of a safer future. We design a MFC-based device. The bacteria can sense many kinds of ions and it can improve the voltage in MFC. We modify the E.coli to make it can produce riboflavin when sensed ions. Our device is designed as an enclosed systems. The measurement of the wastewater is safe, and neither the genetically modified sensor cells nor the wastewater is impossible to leak at all.


Our device can be very helpful in many fields. For more information, please contacted with Xiangkai Li




Part number Part number Where did us get the physical DNA for this part What is the Risk Group of the species? How will you use it?
1 pSB1C3 From Li Xiangkai's Lab, Lanzhou University 1 Vector for E.coli to express BBA_K1755301 Submission vector for all parts
2 K1755001 Synthetize 1 Every parts' foundation
3 K1755002 Parts construction 1 Foundation of other parts
4 K1755003 Parts construction 1 Foundation of other parts
5 K1755004 Parts construction 1 Testing riboflavin expression quantity
6 K1755005 Parts construction 1 Testing riboflavin expression quantity
7 K1755006 Parts construction 1 Testing riboflavin expression quantity
8 K1755007 Parts construction 1 Testing riboflavin expression quantity
9 K1755008 Parts construction 1 Testing riboflavin expression quantity
10 K1755009 Parts construction 1 Testing riboflavin expression quantity
11 K1755301 Synthetize 1 Used in MFC
12 K1755302 Synthetize 1 Used in MFC
13 K1755303 Synthetize 1 Used in MFC
14 K1755305 Synthetize 1 Used in MFC
15 K1755024 Parts construction 1 Used in MFC

 

  Part number Where did us get the physical DNA for this part What species does this part originally come from?  Natural function of part What is the Risk Group of the species? How will you use it?
 1 pSB1C3 From Li Xiangkai's Lab, Lanzhou University Escherichia coli K-12

 Vector for Escherichia coli      1
  1. Vector for E.coli to express BBA_K1523101
  2. Submission vector for all parts
 2 K1523101

 From distributed kit of iGEM 2013
BBA­_K381001		 Escherichia coli K-12 Detector of Nitrate      1 1.  Strain used in anode of MFC 2.  Detector of PNP
3.  Riboflavin producing
 3 pET-28(a) + From Li Xiangkai's Lab, Lanzhou University Vector for Escherichia coli Vector for Escherichia coli      1 Vector for E.coli to express BBA_K1523101
 4 BBa_K1523008 Lanzhou University Escherichia coli

Chromate reduction

      1 1.  Strain used in anode of MFC 2.  Chromate reduction in anode
 5 BBa_K1523021 Lanzhou University Pseudomonas fluorescens

Chromate reduction

      1 1.  Strain used in cathode of MFC
2.  Other organic pollutant reduction in cathode
 6 BBa_K1523022 Lanzhou University Pseudomonas fluorescens Chromate reduction      1

1.  Strain used in cathode of MFC
2.  Other organic pollutant reduction in cathode
 7 BBa_K1523023 Lanzhou University Pseudomonas fluorescens

Chromate reduction

      1 1.  Strain used in cathode of MFC
2.  Other organic pollutant reduction in cathode
 8 BBa_K1523025 Lanzhou University Pseudomonas putida Chromate reduction      1 1.  Strain used in anode of MFC 2.  Chromate reduction in anode