Difference between revisions of "Team:ZJU-China/Notebook"

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    <h2 style="color:green;font-size:4em;">Notebook</h2>
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September
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<p><small class="text-muted">9.5.2015</small></p>
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<p>Finished the overview of entire project.
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<p><small class="text-muted">9.4.2015</small></p>
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<li><p class="p1">Bacteria solution PCR: OrfX. One positive clone of 20 samples.</p></li>
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<li><p class="p1">DNA sequencing: OrfX.</p></li>
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<p>Watched the military parade during the commemoration activities to mark the 70th anniversary of the victory of the Chinese People's War of Resistance Against Japanese Aggression and the World Anti-Fascist War in our lab.
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<p><small class="text-muted">9.2.2015</small></p>
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<p>Dynamic Light Scattering(DLS) experiment to measure the particle size of embedded E.coli.
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<p><small class="text-muted">9.1.2015</small></p>
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<p>The sequence of OrfX was not correct because of the wrong template, thus we had to do the related experiments again.
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August
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<p><small class="text-muted">8.31.2015</small></p>
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<p>Took photos and prepared for the banner~</p>
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<p>Took photos and prepared for the banner~</p>
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<p><small class="text-muted">8.30.2015</small></p>
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<p>The sequence of metK and tcdA1 were confirmed, which marked the success in standardization of 2 parts!!</p>
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<p><small class="text-muted">8.29.2015</small></p>
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<p>Bacteria solution PCR: metK and OrfX. Both had positive clones. DNA sequencing: metK and OrfX.  T4 ligation and transformation: plu0840+pSB1C3, plu1537+pSB1C3, tcdA1+pSB1C3.  Selected from single colonies: CDS-tcdA1.</p>
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<p><small class="text-muted">8.28.2015</small></p>
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<li><p class="p1">Selected from single colonies of metK and OrfX.</p></li>
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<li><p class="p1">Submit the safety form. Selected from single colonies: CDS-plu0840. Double enzyme digestion and DNA gel extraction: plu1537, tcdA1, plu0840.  Plasmid extration and double enzyme digestion: RFP. Protein extraction and SDS-PAGE: tcdA1, plu1537, plu0840. T4 ligation and transformation: plu0840+pSB1C3, plu1537+pSB1C3, tcdA1+pSB1C3. Bacteria solution PCR: CDS-plu0840.</p></li>
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<p><small class="text-muted">8.27.2015</small></p>
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<p>Seamless assembly: plasmid+OrfX, plasmid+metK. PCR: CDS-tcdA1. Double enzyme digestion: RFP+plasmid. Seamless assembly: CDS-plu0840. PCR and DNA gel extraction: CDS-tcdA1. Changed pSB1A3 to pSB1C3: plu0840, plu1537, tcdA1. Transformed them into E.coli DH5α, selected from single colonies and used single enzyme digestion to verify the got plasmids.</p>
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<p>Seamless assembly: plasmid+CDS-tcdA1, plasmid+CDS-plu0840. DNA gel extraction: tcdA1. PCR: device-plu0840.</p>
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<p><small class="text-muted">8.25.2015</small></p>
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<p>The sequence of ermE+plasmid, Frr+plasmid and plu1537+plasmid were confirmed, which marked the success in standardization of 3 parts!!</p>
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<li><p class="p1">Bacteria solution PCR: metK and OrfX. Only negative clone. Extraction of plasmid mCherry and double enzyme digestion. DNA sequencing: ermE+plasmid, Frr+plasmid, plu1537+plasmid. Selected from single colonies of plu1537.</p></li>
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<li><p class="p1">Power cut. We played table tennis near our lab.</p></li>
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<li><p class="p1">Selected from single colonies of metK and OrfX.</p></li>
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<li><p class="p1">Transformed standard plu1537 plasmid into BL-21. Bacterium solution PCR: ermE and Frr.</p></li>
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<li><p class="p1">We kept a Madagascar cockroach as our pet~</p></li>
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<p><small class="text-muted">8.22.2015</small></p>
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<li><p class="p1">Selected from single colonies of ermE and Frr. Extraction of plasmid (standard plu1537).</p></li>
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<li><p class="p1">DNA sequencing. Seamless assembly: plasmid+1537, plasmid+tcdA1-L+ tcdA1-M+ tcdA1-R, plasmid+ermE, plasmid+Frr. </p></li>
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<li><p class="p1">Successfully booked the HI-Boston Hostel and paid the full payment~ Seamless assembly: plasmid+metK, plasmid+OrfX. </p></li>
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<h4>38th Group meeting</h4>
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<p><small class="text-muted">8.20.2015</small></p>
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<p>38th Group meeting. Introduced a software named Mou which could enhance the efficiency of writing wiki. Discussed the business planning of our product and the photos on the wiki. </p>
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<li><p class="p1">Extraction of plasmid mCherry and double enzyme digestion. Seamless assembly: plasmid+1537, plasmid+tcdB1. PCR: ermE, Frr, metK and OrfX. Clean up and DNA gel extraction. </p></li>
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<li><p class="p1">Capsulated Streptomyces Avermitilis toxicity test. TT01 toxicity verification.</p></li>
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<li><p class="p1">PCR: ermE, Frr, metK and OrfX. Clean up and DNA gel extraction. </p></li>
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<li><p class="p1">Again used flow cytometry to check the diameter of the embedded E.coli and tried to count the E.coli which were successfully embedded. The results were not that ideal again.</p></li>
+
−
</ul>
+
−
</div>
+
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</div>
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+
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<p><small class="text-muted">8.19.2015</small></p>
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</div>
+
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<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">PCR: ermE, Frr, metK and OrfX. Clean up and DNA gel extraction. </p></li>
+
−
<li><p class="p1">Finished the background of avermectin and toxic protein. Sent bacterium sample to BNU and we would continue to help them.</p></li>
+
−
</ul>
+
−
</div>
+
−
<br>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/c/c6/8-19.jpg/800px-8-19.jpg" class="img-responsive">
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<p><small class="text-muted">8.18.2015</small></p>
+
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</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">PCR: CDS tcdB1, CDS 1537, tcdA1-L, mcherry. DNA sequencing.</p></li>
+
−
<li><p class="p1">Half-done the model of degration of toxic protein. Got the kit for detection avermectin.</p></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
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<li class="entry">
+
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<div class="timeline-panel">
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<div class="tl-heading">
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<p><small class="text-muted">8.17.2015</small></p>
+
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</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">PCR: CDS tcdB1, CDS plu1537 and mCherry. DNA gel extraction: tcdA1-L and plu0840L. Seamless assembly: plasmid+pBad+plu1537.</p></li>
+
−
<li><p class="p1">Detected the relationship between distance of food source and food consumption.</p></li>
+
−
<li><p class="p1">Designed the primers for Frr, metK, OrfX and ermE again because the need for standardization.</p></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
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+
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+
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<h4>37th Group meeting</h4>
+
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<p><small class="text-muted">8.16.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>37th Group meeting. Built the framework for wiki. Discussed the Syn-bio talks on next Saturday.</p>
+
−
</div>
+
−
 
+
−
</div>
+
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</li>
+
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+
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<li class="entry">
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<div class="timeline-panel">
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<div class="tl-heading">
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<p><small class="text-muted">8.16.2015</small></p>
+
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</div>
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<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">Preparation of competent cells.DNA gel extraction.</p></li>
+
−
<li><p class="p1">Verification of the bait attractiveness.</p></li>
+
−
</ul>
+
−
</div>
+
−
 
+
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</div>
+
−
</li>
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<p><small class="text-muted">8.15.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">PCR: pBad, tcdA1-L, tcdB1, plu0840L and plu1537. Purchased E.coli BL21.</p></li>
+
−
<li><p class="p1">The experiment concerning Streptomyces avermitilis finished 80%.</p></li>
+
−
<li><p class="p1">Lovely poppy~ </p></li>
+
−
</ul>
+
−
</div>
+
−
<br>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/c/cd/8-15.jpg/450px-8-15.jpg" class="img-responsive">
+
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+
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+
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</div>
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</li>
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+
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<li class="entry">
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<div class="tl-heading">
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<p><small class="text-muted">8.14.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">PCR and clean up. Concluded the successful PCR parameters of different gene clusters.</p></li>
+
−
<li><p class="p1">Test the Avermectin detection kit with pure Ivermectin.</p></li>
+
−
<li><p class="p1">Designed the primers for plu0840, plu1537, tcdA1 and tcdB1 again bucause of the need for standardization.</p></li>
+
−
</ul>
+
−
</div>
+
−
<br>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/a/a3/%E5%B1%8F%E5%B9%95%E5%BF%AB%E7%85%A7_2015-09-08_%E4%B8%8B%E5%8D%8811.50.11.png/800px-%E5%B1%8F%E5%B9%95%E5%BF%AB%E7%85%A7_2015-09-08_%E4%B8%8B%E5%8D%8811.50.11.png" class="img-responsive">
+
−
</div>
+
−
</li>
+
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+
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<li class="entry timeline-inverted">
+
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<div class="tl-heading">
+
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<p><small class="text-muted">8.13.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">Overlap: pBad and tcdA1-L, pBad and tcd-B1, pBad and plu1537. Extraction of plasmid mCherry and enzyme digestion.</p></li>
+
−
<li><p class="p1">Completed wiki page for 2nd model. Drew the 3D ball-and-stick models for CNC with Solidworks. Completed the device for killing termites. </p></li>
+
−
<li><p class="p1">Yummy hotpot~</p></li>
+
−
<li><p class="p1">Completed the SCM program for HAZU and they affirmed our help.</p></li>
+
−
</ul>
+
−
</div>
+
−
<br>
+
−
<img src="https://static.igem.org/mediawiki/2015/0/0e/8-13.jpg" class="img-responsive">
+
−
<br><br>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/3/3b/8-13-2.jpg/450px-8-13-2.jpg" class="img-responsive">
+
−
</div>
+
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</li>
+
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+
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<li class="entry">
+
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<div class="timeline-panel">
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<div class="tl-heading">
+
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<p><small class="text-muted">8.12.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">Overlap: 0840L and 0840R.</p></li>
+
−
<li><p class="p1">Tried to purify the embedded E.coli with the E.coli which were able to express RFP.</p></li>
+
−
<li><p class="p1">Used flow cytometry to check the diameter of the embedded E.coli and tried to count the E.coli which were successfully embedded. The results were not that ideal.</p></li>
+
−
</ul>
+
−
</div>
+
−
<br>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/d/d3/%E6%B5%81%E5%BC%8F.png/800px-%E6%B5%81%E5%BC%8F.png" class="img-responsive">
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
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<div class="timeline-panel">
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<div class="tl-heading">
+
−
<p><small class="text-muted">8.11.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">PCR: pBad, tcdA1-L, tcdA1-M, tcdA1-R, tcdB1, 0840L, 0840R, 1537, GFP.</p></li>
+
−
<li><p class="p1">PCR: ligated product. Optimized the PCR program. Cleaned up. PCR again.  </p></li>
+
−
<li><p class="p1">SEM scanned our samples to verify whether Streptomyces avermitilis were successfully embedded with CNC.</p></li>
+
−
<li><p class="p1">Fed the termites with Photorhabdus luminescens TT01 bacterium cells.</p></li>
+
−
</ul>
+
−
</div>
+
−
<br>
+
−
<img src="https://static.igem.org/mediawiki/2015/4/4c/8-11.png" class="img-responsive">
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
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<div class="tl-heading">
+
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<p><small class="text-muted">8.10.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">PCR: pBad, tcdA1-L and GFP.</p></li>
+
−
<li><p class="p1">Used Bgl2 and EcoR1 digested inserted fragment and cleaned up the product. Ligating fragment into plasmid with T4 ligase.</p></li>
+
−
<li><p class="p1">Specified the standardization requirements and laid the foundation for primer designing.</p></li>
+
−
<li><p class="p1">Mixed blue-stained termites with red-stained termites to verify trophallaxis.</p></li>
+
−
</ul>
+
−
</div>
+
−
<br>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/f/f6/8-10.png/470px-8-10.png" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>35th Group meeting</h4>
+
−
<p><small class="text-muted">8.9.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>35th Group meeting. Planned to hold a seminar for freshmen. Tried to design a device for attracting and killing termites.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
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<div class="timeline-panel">
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<div class="tl-heading">
+
−
<p><small class="text-muted">8.9.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">PCR: tcdA1-M, tcdA1-R, tcdB1, plu0840L, plu0840R and plu1537.</p></li>
+
−
<li><p class="p1">Completed our framework and philosophy of safety.</p></li>
+
−
<li><p class="p1">Extracted the plasmid containing RFP from DH5α. PCR: RFP and confirmed by gel electrophoresis.</p></li>
+
−
</ul>
+
−
</div>
+
−
<br>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/3/3f/8-9.jpg/800px-8-9.jpg" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">8.8.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/3/38/8-8-2.png/800px-8-8-2.png" class="img-responsive">
+
−
</div>
+
−
</li>
+
−
 
+
−
 
+
−
<li class="entry timeline-inverted">
+
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<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">8.8.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/b/bb/8-8-3.jpg/800px-8-8-3.jpg" class="img-responsive">
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/8/8f/8-8.jpeg/800px-8-8.jpeg" class="img-responsive">
+
−
</div>
+
−
</li>
+
−
 
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">8.7.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>One step cloning protocol finished. Designed the questionaire about termites.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">8.7.2015-8.8.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>PCR: pBad, GFP, tcdA1-M, tcdA1-R.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>34th Group meeting</h4>
+
−
<p><small class="text-muted">8.6.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>34th Group meeting. Communicated with 2 members of SJTU in our lab. Began to write wiki. Attached more importance to safety.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">8.6.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">Drawing plasmid circuits in snapgene.(toxic protein)</p></li>
+
−
<li><p class="p1">Stained 50 termites in blue and 50 in red for trophallaxis experiments.</p></li>
+
−
</ul>
+
−
</div>
+
−
<br>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/e/ed/8-6.png/800px-8-6.png" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">8.5.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">Discussed the circuit construction and experiments planning of toxic protein.</p></li>
+
−
<li><p class="p1">Decided to give up confocol because we couldn't find the specific dye for CNC.</p></li>
+
−
</ul>
+
−
</div>
+
−
<br>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/c/c7/8-5.jpg/450px-8-5.jpg" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
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<div class="tl-heading">
+
−
<p><small class="text-muted">8.4.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Completed the One Step Cloning protocol.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/e/e1/8-4.png/494px-8-4.png" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>33rd Group meeting</h4>
+
−
<p><small class="text-muted">8.2.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>33rd Group meeting. Began to build framework for wiki. Introduced the habits and characteristics of termites.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="month" id="July"><div class="tldate" style="font-family:"gennifer",Verdana,Sans-serif"><div class="div-in">
+
−
July
+
−
</div></div></li>
+
−
 
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>32nd Group meeting</h4>
+
−
<p><small class="text-muted">7.30.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>32nd Group meeting. Planned to promote synthetic biology and our project in Shanghai Science & Technology Museum. Polished up the Synbio-cards(Polypoly card).</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">7.28.2015-8.4.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>PCR: pBad, tcdA1-L, tcdA1-M, tcdA1-R, tcdB1, plu0840L, plu0840R, plu1537 and GFP.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">7.28.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Giant Jamboree registration completed.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/e/e5/7-28.png/420px-7-28.png" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">7.26.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">Listed 15 main problems we were confronted with, including safety, communication between dry group and wet groups.</p></li>
+
−
<li><p class="p1">Designed a new method to embed the bacterium with CNC.</p></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">7.25.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Designed the primers for plu0840, plu1537, tcdA1 and tcdB1 again bucause of the bug of extra RBS.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">7.24.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Discussed helping BNU and HUST. Designed and alpha tested the Synbio-cards. Improved the modeling.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">7.19.2015-7.24.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST. </p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/b/bc/7-20.jpg/800px-7-20.jpg" class="img-responsive">
+
−
</div>
+
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</li>
+
−
 
+
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+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
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<div class="tl-heading">
+
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<p><small class="text-muted">7.19.2015-7.24.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST. </p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/3/3b/7-19.jpg/800px-7-19.jpg" class="img-responsive">
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">7.18.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">Listed and detailed the wiki requirements.</p></li>
+
−
<li><p class="p1">Fed the termites with Streptomyces Avermitilis bacterium cells.</p></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
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−
<div class="tl-heading">
+
−
<p><small class="text-muted">7.16.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Prepared the home page and member page of our wiki. Prepared the poster for NCTU meetup.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/4/42/7-16.png/338px-7-16.png" class="img-responsive">
+
−
 
+
−
</div>
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−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
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−
<div class="tl-heading">
+
−
<p><small class="text-muted">7.15.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">Designed the primers for Frr, metK, OrfX and ermE again. Got our name card~</p></li>
+
−
<li><p class="p1">Used confocal to verify the successful package of E.coli with CNC. However, the result appeared not ideal and we couldn't find appropriate dye of CNC.</p></li>
+
−
</ul>
+
−
</div>
+
−
<br>
+
−
<img src="https://static.igem.org/mediawiki/2015/5/51/7-15.png" class="img-responsive">
+
−
 
+
−
</div>
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−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">7.14.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Prepared for the souvenirs for NCTU meetup.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>31st Group meeting</h4>
+
−
<p><small class="text-muted">7.12.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>31st Group meeting. Reviewed the medal requirement and all the rules in lab.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/e/e8/7-12.png/605px-7-12.png" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">7.11.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Mark-recapture method failed because we failed to recapture enough stained termites.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">7.3.2015-7.11.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Exams of summer semester.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="month" id="June"><div class="tldate" style="font-family:"gennifer",Verdana,Sans-serif"><div class="div-in">
+
−
June
+
−
</div></div></li>
+
−
 
+
−
 
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>30th Group meeting</h4>
+
−
<p><small class="text-muted">6.25.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>30th Group meeting. Planned to verify the package of CNC with confocal. Continued to prepare for the presentation and application scenario.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.24.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">Completed 2 safety questionaires.</p></li>
+
−
<li><p class="p1">Prepared to learn confocol to verify the CNC package for E.coli.</p></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.22.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Put 150 blue stained termites back into the nest for mark-recapture method.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>29th Group meeting</h4>
+
−
<p><small class="text-muted">6.21.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>29th Group meeting. Discussed the application scenario for the 5000€ additional sponsership of SYNENERGENE.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.21.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">Contacted with Björn and he would help us animate the process of crystallization.
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.20.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>TEM scanned our samples.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.19.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Fed the termites with Photorhabdus luminescens TT01 bacterium solution to test the natural toxicity.
+
−
Stained 150 termites in blue for future experiment.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>28th Group meeting</h4>
+
−
<p><small class="text-muted">6.18.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>28th Group meeting. 2nd modeling half done. Discussed the results of the SEM.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.17.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>TEM and SEM scanned our samples.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/8/8a/6-17-3.png/798px-6-17-3.png" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.17.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>TEM and SEM scanned our samples.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/7/74/6-17-2.jpg/590px-6-17-2.jpg" class="img-responsive">
+
−
<img src="https://static.igem.org/mediawiki/2015/a/a4/6-17.jpg" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.15.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Fed the termites with Streptomyces Avermitilis bacterium solution to test the natural toxicity.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>27th Group meeting</h4>
+
−
<p><small class="text-muted">6.14.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>27th Group meeting. Rearranged all the events with four quadrant method. Checked everything we have had. </p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/7/70/6-14.jpg/800px-6-14.jpg" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.14.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">Solved the diffusion equation.
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/3/3d/6-14-2.jpg" class="img-responsive">
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/b/ba/6-14-3.png/800px-6-14-3.png" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.13.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Extracted the genome of Streptomyces avermitilis.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.12.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Went to shanghai for the interview of visa. 12 members successfully passed the interview. </p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>26th Group meeting</h4>
+
−
<p><small class="text-muted">6.11.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>26th Group meeting. Discussed the half-done presentation and Skype conference with SYNENERGENE. Prepared for the visa.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.11.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">PCR: plu1537, with the template of the genome of TT01.
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.10.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">Paid for the air tickets.</p></li>
+
−
<li><p class="p1">Got the primers of toxic protein.</p></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.8.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Optimization of the model of termites finding food.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/8/82/6-8-2.png" class="img-responsive">
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/2/27/6-8-3.png/800px-6-8-3.png" class="img-responsive">
+
−
<img src="https://static.igem.org/mediawiki/2015/1/1b/6-8.png" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>25th Group meeting</h4>
+
−
<p><small class="text-muted">6.7.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>25th Group meeting. Polished up the previous modeling. Protocols about the experiments concerning bacterium. A member quit.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.7.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">Extracted the genome of TT01.
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.6.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Frozen the spores of Streptomyces avermitilis.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/0/03/6-6.jpeg/800px-6-6.jpeg" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>24th Group meeting</h4>
+
−
<p><small class="text-muted">6.4.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>24th Group meeting. Brainstormed the Synbio-cards and Synbio-workshops. Listed the datailed personal scheme about the time arrangement before summer holiday.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/9/98/6-4-2.png/525px-6-4-2.png" class="img-responsive">
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/4/41/6-4.jpg/692px-6-4.jpg" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.3.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">Discussed the characterization of E.coli embedded in CNC, including TEM, SEM, confocal, FTIR and flow cytometry.</p></li>
+
−
<li><p class="p1">Our proposal had been accepted and our team would be awarded financial support from SYNENERGENE!  Cheers~</p></li>
+
−
<li><p class="p1">Brainstormed how to do human practice and use the €5000. We came up with the  Synbio-cards and Synbio-workshops.</p></li>
+
−
</ul>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/8/8d/6-3.png/800px-6-3.png" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.2.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Began to book the air tickets. Booked the first primers of toxic protein for one step cloning. Found the sequence of the gene we need in the Streptomyces avermitilis.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.1.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>SEM scanned our samples again. Debugged the first modeling. </p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="month" id="May"><div class="tldate" style="font-family:"gennifer",Verdana,Sans-serif"><div class="div-in">
+
−
May
+
−
</div></div></li>
+
−
 
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>23rd Group meeting</h4>
+
−
<p><small class="text-muted">5.31.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>23rd Group meeting. Discussed the half-done modeling.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">5.31.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">Built up the model of termites finding food.
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/7/7a/5-31-2.png" class="img-responsive">
+
−
</div>
+
−
</li>
+
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<p><small class="text-muted">5.31.2015</small></p>
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</div>
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<div class="tl-body">Taught high school students to do experiment to extract DNA from epithelial cell of mouth.
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</div>
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<img src="https://static.igem.org/mediawiki/2015/thumb/b/b7/5-31-3.jpg/800px-5-31-3.jpg" class="img-responsive">
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<br><br>
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<img src="https://static.igem.org/mediawiki/2015/thumb/f/f5/5-31-2.jpg/800px-5-31-2.jpg" class="img-responsive">
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<br><br>
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<img src="https://static.igem.org/mediawiki/2015/thumb/7/78/5-31.jpg/800px-5-31.jpg" class="img-responsive">
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<p><small class="text-muted">5.29.2015</small></p>
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</div>
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<div class="tl-body">
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<ul>
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<li><p class="p1">Repeated the cellulase activity detection experiment.
+
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Meanwhile we measured the lysozyme activity in different parts of termites guts.</p></li>
+
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<li><p class="p1">22nd Group meetig. Specified the medal requirement. Began to prepare for the  NCTU meetup.</p></li>
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<li><p class="p1">SEM scanned our samples. </p></li>
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−
</ul>
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</div>
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<p><small class="text-muted">5.28.2015</small></p>
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</div>
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<div class="tl-body">
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<p>Completed a model of a cartoon termite holding a sword. Cleaned up our lab and rearranged all the experiment materials we would need.</p>
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</div>
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</div>
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</li>
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<p><small class="text-muted">5.26.2015</small></p>
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</div>
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<div class="tl-body">
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<p>Got the solid CNC with lyophilization. Built the framework of the first web page.</p>
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</div>
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<img src="https://static.igem.org/mediawiki/2015/thumb/0/0b/5-26.png/800px-5-26.png" class="img-responsive">
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<p><small class="text-muted">5.25.2015</small></p>
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</div>
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<div class="tl-body">
+
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<p>Learned the skills of culturing Streptomyces avermitilis in the professor's lab.</p>
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</div>
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</div>
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<li class="entry timeline-inverted">
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<h4>21st Group meeting</h4>
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<p><small class="text-muted">5.24.2015</small></p>
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</div>
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<div class="tl-body">
+
−
<p>21st Group meeting. Began to prepare for the presentation. </p>
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</div>
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</div>
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</li>
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<li class="entry">
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<p><small class="text-muted">5.22.2015</small></p>
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</div>
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<div class="tl-body">
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−
<p>iGEM Shanghai Tour. Took part in the conference held in NYU. Visited FDU and communicated with them.</p>
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</div>
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</div>
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</li>
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<li class="entry timeline-inverted">
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<div class="tl-heading">
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<h4></h4>
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<p><small class="text-muted">5.21.2015</small></p>
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</div>
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<div class="tl-body">
+
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<p>Chosen the certain toxic protein which we would transfer into E.coli: tcdA1, tcdB1,plu0840 and plu1537.</p>
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</div>
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</div>
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</li>
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<li class="entry">
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<div class="timeline-panel">
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<div class="tl-heading">
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<p><small class="text-muted">5.21.2015</small></p>
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</div>
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<div class="tl-body">20th Group meeting. Discussed the medal requirements and collaboration among groups.
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</div>
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</div>
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</li>
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−
 
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<li class="entry">
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<div class="timeline-panel">
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<div class="tl-heading">
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<p><small class="text-muted">5.20.2015</small></p>
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</div>
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−
<div class="tl-body">
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−
<ul>
+
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<li><p class="p1">Finished the protocol about TT01.</p></li>
+
−
<li><p class="p1">Measured the cellulase activity in termites head, forgut, midgut and hindgut.</p></li>
+
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</ul>
+
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</div>
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−
</div>
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</li>
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−
 
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<li class="entry">
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<div class="tl-heading">
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<p><small class="text-muted">5.19.2015</small></p>
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</div>
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−
<div class="tl-body">
+
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<p>Detailed the medal requirements depending on our project. Finished the protocol about Streptomyces avermitilis.</p>
+
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</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/8/89/5-19-2.png/800px-5-19-2.png" class="img-responsive">
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<br><br>
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<img src="https://static.igem.org/mediawiki/2015/thumb/8/89/5-19-2.png/800px-5-19-2.png" class="img-responsive">
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+
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</div>
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</li>
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<li class="entry">
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<div class="timeline-panel">
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<div class="tl-heading">
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<p><small class="text-muted">5.18.2015</small></p>
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</div>
+
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<div class="tl-body">
+
−
<p>Got the E.coli strain ET12567 from Professor Li's Lab. Began the experiment about Streptomyces avermitilis.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
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<li class="entry timeline-inverted">
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<div class="timeline-panel">
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<div class="tl-heading">
+
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<h4>18th Group meeting</h4>
+
−
<p><small class="text-muted">5.17.2015</small></p>
+
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</div>
+
−
<div class="tl-body">
+
−
<p>18th Group meeting. Emphasized the lab safety. </p>
+
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</div>
+
−
</div>
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</li>
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−
 
+
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<li class="entry">
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<div class="tl-heading">
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<p><small class="text-muted">5.17.2015</small></p>
+
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</div>
+
−
<div class="tl-body">Did experiments(rainbow kit)related to synthetic biology in Zhejiang Science&Technology Museum with high school students and by this way promoted syn-bio.
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/a/a3/5-17.jpg/800px-5-17.jpg" class="img-responsive">
+
−
 
+
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</div>
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</li>
+
−
 
+
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<li class="entry">
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<div class="timeline-panel">
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<div class="tl-heading">
+
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<p><small class="text-muted">5.15.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Read paper and discussed how to enhance the efficiency of Avermectin in Streptomyces avermitilis.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/4/4a/5-15.png/800px-5-15.png" class="img-responsive">
+
−
 
+
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</div>
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</li>
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+
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<li class="entry timeline-inverted">
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<div class="timeline-panel">
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<div class="tl-heading">
+
−
<h4>17th Group meeting</h4>
+
−
<p><small class="text-muted">5.14.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>17th Group meeting. Discussed the results of the TEM. Tried to contact with a judge from PKU.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
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<div class="timeline-panel">
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<div class="tl-heading">
+
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<p><small class="text-muted">5.14.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">TEM scanned our samples: using CNC to pack the Bacillus subtilis.</p></li>
+
−
<li><p class="p1">Completed the cover and comic of our proposal which would be handed in to SYNENERGENE.</p></li>
+
−
<li><p class="p1">Contacted with Hangzhou Termites control Institute.</p></li>
+
−
</ul>
+
−
</div>
+
−
<br>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/f/f4/5-14-3.jpg/800px-5-14-3.jpg" class="img-responsive">
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/4/48/5-14.jpg/590px-5-14.jpg" class="img-responsive">
+
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</div>
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</li>
+
−
 
+
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<li class="entry">
+
−
<div class="timeline-panel">
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−
<div class="tl-heading">
+
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<p><small class="text-muted">5.14.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">TEM scanned our samples: using CNC to pack the Bacillus subtilis.</p></li>
+
−
<li><p class="p1">Completed the cover and comic of our proposal which would be handed in to SYNENERGENE.</p></li>
+
−
<li><p class="p1">Contacted with Hangzhou Termites control Institute.</p></li>
+
−
</ul>
+
−
</div>
+
−
<br>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/0/06/5-14-2.jpg/590px-5-14-2.jpg" class="img-responsive">
+
−
</div>
+
−
</li>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
<li class="entry">
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<div class="timeline-panel">
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<div class="tl-heading">
+
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<p><small class="text-muted">5.13.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">Got the genome of tcdA1 and tcdB1 in NCBI.</p></li>
+
−
<li><p class="p1">Got TT01(a species of Photorhabdus luminescens) which could produce toxic protein. It would play an important role in our plan B.</p></li>
+
−
</ul>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/c/c3/5-13.JPG/800px-5-13.JPG" class="img-responsive">
+
−
</div>
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</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">5.12.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Successfully checked in termites and tc protein family.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/0/02/5-12.png" class="img-responsive">
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>16th Group meeting</h4>
+
−
<p><small class="text-muted">5.10.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>16th Group meeting. Communication between dry group and 3 wet groups. Arranged time for experiments.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
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−
<div class="tl-heading">
+
−
<h4>15th Group meeting</h4>
+
−
<p><small class="text-muted">5.7.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>15th Group meeting. Further discussed the proposal and allocated the tasks. Continued to consult professors about several species of bacterium.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
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<div class="timeline-panel">
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−
<div class="tl-heading">
+
−
<p><small class="text-muted">5.6.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p class="p1">Gave up synthesizing avermectin in Bacillus subtilis. Tried to overexpress avermectin in E.coli and then transformed its plasmid into Streptomyces avermitilis.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/e/e4/5-6-2.jpg/800px-5-6-2.jpg" class="img-responsive">
+
−
</div>
+
−
</li>
+
−
 
+
−
 
+
−
<li class="entry">
+
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<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">5.6.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p class="p1">Successfully got the solution of CNC!! And it appeared Tyndall effect.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/c/c1/5-6.jpg/800px-5-6.jpg" class="img-responsive">
+
−
</div>
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</li>
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−
<li class="entry">
+
−
<div class="timeline-panel">
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−
<div class="tl-heading">
+
−
<p><small class="text-muted">5.4.2015-5.6.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Exams of spring semester.</p>
+
−
</div>
+
−
</div>
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−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">5.3.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Our termite nest was sent out from Guangdong Province.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="month" id="April"><div class="tldate" style="font-family:"gennifer",Verdana,Sans-serif"><div class="div-in">
+
−
April
+
−
</div></div></li>
+
−
 
+
−
 
+
−
<li class="entry timeline-inverted">
+
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<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>14th Group meeting</h4>
+
−
<p><small class="text-muted">4.29.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>14th Group meeting. 10 members would go to Taiwan for NCTU meetup. Results of consulting professors specialized in termites and Bacillus subtilis.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/a/ad/4-29.png" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">4.28.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<ul>
+
−
<li><p class="p1">Discussed the plan for embedding baterium with CNC and the self-assembly of CMC and chitosan on the surface of baterium.</p></li>
+
−
<li><p class="p1">Debugged the problem of synthesizing avermectin in Streptomyces avermitilis. Consulted professors specialized in synthesizing avermectin in Streptomyces avermitilis.</p></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">4.27.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Consulted professors specialized in termites and Bacillus subtilis.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<h4>13th Group meeting</h4>
+
−
<p><small class="text-muted">4.26.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>13th Group meeting. Discussed something important: the proposal for the 5000€ additional sponsership of SYNENERGENE and visa. A new mamber joined our team. Specified the problem remaining to be solved of each group.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">4.25.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Discussd the advantage of Bacillus subtilis campared with E.coli and the utilization of spore of Bacillus subtilis.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry timeline-inverted">
+
−
<div class="timeline-panel">
+
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<div class="tl-heading">
+
−
<h4>12th Group meeting</h4>
+
−
<p><small class="text-muted">4.23.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>12th Group meeting. Debugged the previous package plan and decided to pack bacterium with CNC. Planned to buy a nest of termites from Guangdong Province. Discussed several demensions of modeling in this project.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/a/a0/4-23-2.jpg/600px-4-23-2.jpg" class="img-responsive">
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/a/a6/4-23.jpg/450px-4-23.jpg" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">4.22.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Consulted professors specialized in termites.</p>
+
−
</div>
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">4.21.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>Consulted professors about directly embedding baterium with CNC rather than with the aid of protein domain. Our idea was confirmed and praised.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/8/81/4-21.png/450px-4-21.png" class="img-responsive">
+
−
 
+
−
</div>
+
−
</li>
+
−
 
+
−
<li class="entry">
+
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">4.20.2015</small></p>
+
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<p>Communication with our instructor. Searched for information about cellulose binding domain(CBDs) and overexpression of ivermectin and avermectin.</p>
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<p>11th Group meeting. Decided our project: termite terminator. Allocated all members into 4 groups. Each group was responsible for a part of our project. </p>
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<p><small class="text-muted">4.16.2015</small></p>
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<div class="tl-body">
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<p>10th Group meeting. Decided to participate in NCTU meetup in summer holiday and apply for the 5000€ additional sponsership of SYNENERGENE. Discussed 3 existing ideas.</p>
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<p>Preliminarily designed the circuit in Streptomyces avermitilis.</p>
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<div class="tl-body">
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<p>9th Group meeting. Kept 3 ideas and the final idea would derive from them. Detailed these 3 ideas from all the aspects.</p>
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<p><small class="text-muted">4.9.2015</small></p>
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<div class="tl-body">
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<p>8th Group meeting. Continued to kill unappropriate ideas. Recalled the aim of participating iGEM and reached a consensus.</p>
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<p>7th Group meeting. Began to kill some undoable ideas and further dug the existing ideas, such as biological enigma machine and killing termites.</p>
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<p>6th Group meeting. Developed several new ideas, including bio-SCM, biological enigma machine and slow release of certain chemical. Talked about their feasibility.</p>
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</div>
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<img src="https://static.igem.org/mediawiki/2015/thumb/5/5f/4-2-2.jpg/800px-4-2-2.jpg" class="img-responsive">
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<p>5th Group meeting. Dug previous ideas and considered their safety and feasibility.</p>
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<p><small class="text-muted">3.26.2015</small></p>
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</div>
+
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<div class="tl-body">
+
−
<p>4th Group meeting. Continued to share and improve our ideas, such as bio-lens, detection of schistosomiasis and killing termites.</p>
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<div class="tl-body">
+
−
<p>Team application completed.</p>
+
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<h4>3rd Group meeting</h4>
+
−
<p><small class="text-muted">3.22.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>3rd Group meeting. Discussed some ideas about modeling. Shared new ideas.</p>
+
−
</div>
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−
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−
 
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+
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<p><small class="text-muted">3.21.2015</small></p>
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</div>
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−
<div class="tl-body">
+
−
<p>Communicate with Zhejiang Science&Technology Museum.</p>
+
−
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+
−
<p><small class="text-muted">3.19.2015</small></p>
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</div>
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<div class="tl-body">
+
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<p>2nd Group meeting. Built up several rules and principles. Tried to make the most of our BBS.</p>
+
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<h4>1st Group meeting</h4>
+
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<p><small class="text-muted">3.16.2015</small></p>
+
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</div>
+
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<div class="tl-body">
+
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<p>1st Group meeting. Everyone was assigned with his/her own mission. Brainstorm began.</p>
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<p><small class="text-muted">3.15.2015</small></p>
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</div>
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<div class="tl-body">
+
−
<p>Visited the AST space of Zhejiang Science&Technology Museum. Decided to collaborate with.</p>
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</div>
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<div class="tl-body">
+
−
<p id="Winter">Concluded the winter project.</p>
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<p><small class="text-muted">2.1.2015-3.13.2015</small></p>
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</div>
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<div class="tl-body">
+
−
<p>Everyone read wikis, learned something from previous projects and attempted to come up with new ideas.</p>
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<p><small class="text-muted">1.31.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>1st meetup. Had a nice meal~</p>
+
−
</div>
+
−
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<p><small class="text-muted">1.30.2015</small></p>
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−
</div>
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−
<div class="tl-body">
+
−
<p>15 members was picked out. ZJU-China 2015 team was set up~ Our story began.</p>
+
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</div>
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<img src="https://static.igem.org/mediawiki/2015/c/c8/1-30-2.jpg" class="img-responsive">
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Revision as of 11:03, 10 September 2015

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   width: 95%;
 }

 ul.timeline li .tl-circ {
   top: 22px;
   left: 22px;
   margin-left: 0;

 }
 ul.timeline > li > .tldate {
   margin: 0;
   text-align: left;
 }

 ul.timeline > li > .timeline-panel {
   float: right;
 }

 ul.timeline > li > .timeline-panel:before {
   border-left-width: 0;
   border-right-width: 15px;
   left: -15px;
   right: auto;
 }

 ul.timeline > li > .timeline-panel:after {
   border-left-width: 0;
   border-right-width: 14px;
   left: -14px;
   right: auto;
 }

}