Difference between revisions of "Team:UiOslo Norway/Experiments/Ni-NTA Affinity Chromatography"

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<h1><i>Ni-NTA Affinity Chromatography</h1>
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<a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments" >
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Back to Protocols
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  <li><p>Equilibriated the Ni-NTA with <i> E. coli </i> raw extract by rolling for 40 minutes.</p></li>
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  <li><p>Transfer mixture to an Econo-Column® Chromatography Columns (BIORAD).</p></li>
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  <li><p>Wash with 100 mL 10 mM imidazolebuffer and collect the fraction.</p></li>
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  <li><p>Wash with 30 mL 50 mM imidazolebuffer and collect the fraction.</p></li>
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  <li><p>Elute with 300 mM imidazolebuffer and collect the fraction.</p></li>
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  <li><p>Check the obtained fractions by SDS-Page.</p></li>
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Revision as of 11:49, 10 September 2015

Ni-NTA Affinity Chromatography

Back to Protocols


  • Equilibriated the Ni-NTA with E. coli raw extract by rolling for 40 minutes.

  • Transfer mixture to an Econo-Column® Chromatography Columns (BIORAD).

  • Wash with 100 mL 10 mM imidazolebuffer and collect the fraction.

  • Wash with 30 mL 50 mM imidazolebuffer and collect the fraction.

  • Elute with 300 mM imidazolebuffer and collect the fraction.

  • Check the obtained fractions by SDS-Page.


    • iGEM UiOslo 2015 was sponsored by: