Difference between revisions of "Team:TU Dresden/Notebook/Protocols/AgaroseGel"

Line 57: Line 57:
 
<ul>
 
<ul>
 
   <li style="margin-bottom: 10px;line-height:1.8;">0.89 M Boric acid (M<sub>r</sub> 121.14).</li>
 
   <li style="margin-bottom: 10px;line-height:1.8;">0.89 M Boric acid (M<sub>r</sub> 121.14).</li>
   <li style="margin-bottom: 10px;line-height:1.8;"></li>
+
   <li style="margin-bottom: 10px;line-height:1.8;">0.89 M Tris (M<sub>r</sub> 61.83).</li>
   <li style="margin-bottom: 10px;line-height:1.8;"></li>
+
   <li style="margin-bottom: 10px;line-height:1.8;">0.02 M EDTA pH 8.0 500mM stock solution pH 8.0.</li>
 
</ul>
 
</ul>
 
0.89 M Boric acid,      Mr 121.14
 
0.89 M Tris,                Mr  61.83
 
0.02 M EDTA pH 8.0  500 mM stock solution pH 8.0
 
  
  

Revision as of 13:44, 10 September 2015


How to prepare a 0.7 % agarose gel

Recipe for 10x TBE buffer:

  • 0.89 M Boric acid (Mr 121.14).
  • 0.89 M Tris (Mr 61.83).
  • 0.02 M EDTA pH 8.0 500mM stock solution pH 8.0.
  1. μL °C