Difference between revisions of "Team:Amoy/Member/Attributions"

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<h2> Attributions</h2>
  
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<title>Aomy/Project</title>
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<p> Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster. </p>
  
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<h4> Can we base our project on a previous one? </h4>
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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<h4> Why is this page needed? </h4>
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
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<p>
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
  
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  <li class='active has-sub'><a href='https://2015.igem.org/Team:Amoy/Member'>Team</a>
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<li>General Support</li>
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<li>Project support and advice</li>
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        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Member#member_student'>Member</a></li>
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<li>Fundraising help and advice</li>
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        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Member/Amoy'>Amoy</a></li>
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<li>Lab support</li>
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        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Member/Attributions'>Attributions</a></li>
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<li>Difficult technique support</li>
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<li>Project advisor support</li>
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<li>Human Practices support</li>
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        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Project/Background'>Background</a></li>
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
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        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Project/Methods'>Methods</a></li>
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        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Project/Results'>Results</a></li>
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        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Project/Discussion'>Discussion</a></li>
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  <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Notebook'>Notebook</a>
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        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Notebook/Protocol'>Protocol</a></li>
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  <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Newsletter'>Newsletter</a>
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        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Newsletter/Contribution'>Contribution</a></li>
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        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Newsletter/Discussion'>Discussion</a></li>
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  <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Practice'>Practice</a>
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        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Practice/Handbook'>Handbook</a></li>
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  <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Judging'>Judging</a>
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        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Judging#medal'>Medal Criteria</a></li>
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        <li class='has-sub'><a href='https://2015.igem.org/Team:Amoy/Judging#acknowledgement'>Acknowledgement</a></li>
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  <li><a href='https://2015.igem.org/Team:Amoy/Safety'>Safety</a></li>
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<h4>Inspiration</h4>
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<p>Take a look at what other teams have done:</p>
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
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<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
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<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
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<a href="https://2015.igem.org/Team:Amoy/Member"><h4>Team</h4></a>
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<li><a class="a_Students" href="https://2015.igem.org/Team:Amoy/Member#member_student">Member</a></li>
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<li><a class="a_Advisor" href="https://2015.igem.org/Team:Amoy/Member#member_advisor">Advisor</a></li>
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<li><a class="a_Instructor" href="https://2015.igem.org/Team:Amoy/Member#member_instructor">Instructor</a></li>
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<li><a href="https://2015.igem.org/Team:Amoy/Member/Amoy">Amoy</a></li>
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<li><a href="https://2015.igem.org/Team:Amoy/Member/Attributions">Attributions</a></li>
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<p id="title_p">PROJECT DESCRIPTION</p>
 
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<p class="main_p">L-tert-leucine is an important and attractive chiral building block. Owning to its bulky and hydrophobic tert-butyl side chain, this unnatural amino acid is widely used as chiral auxiliaries and catalysts in asymmetric synthesis in developing chiral pharmaceutically active chemicals. Many methods, such as strecker synthesis, amidocarbonylation and acetamidomolonic ester synthesis, have been used in  L-tert-leucine synthesis, but products are usually racemic. In order to solve the problem, scientists developed enzymatic reductive amination to product L-tert-leucine by using leucine dehydrogenase and formate dehydrogenase. This technology greatly improve the yield and excellent enantiomeric excess of L-tert-leucine.</p>
 
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<p class="main_p">Initially, they used isolated enzymes, which can be disadvantageous for the reason that enzymes are  always destabilized in the isolation and purification process. What's more, the cofactor-NADH is rather an expensive raw material, which will enhance the cost of L-tert-leucine production. So scientists introduced whole-cell biocatalysts to L-tert-leucine production. Whole-cell biocatalysts could stabilize enzymes and reduce the addition level of cofactor NADH.</p>
 
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<p class="main_p">However, owing to different strength of leucine dehydrogenase and formate dehydrogenase, the NADH consumption rate does not equal to its regeneration. Therefore, it is necessary to add excess NADH. Many different methods have been used, but none of them made difference. Because the criminal is different strength of enzymes, we want to regulate the efficiency of ribosome binding site (RBS) to control the strength of leucine dehydrogenase. With the help of mathematical modeling, we will get the most suitable efficiency of RBS of leucine dehydrogenase. As a result, the addition of excess NADH could be decreased.
 
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<p style="font-size: 16px; color: #fff; border-bottom: 1px solid #fff; padding-bottom: 5px; margin-bottom: 0px;">CONTACT US</p>
 
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<p style="font-size: 14px; color: #fff; margin-top: 5px; margin-bottom: 0px;"><strong>Email:  </strong>igemxmu@gmail.com</p>
 
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<p style="font-size: 14px; color: #fff; margin-bottom: 0px;"><strong>Website:  </strong><a href="https://2015.igem.org/Team:Amoy" target="_blank" style="color: #fff;">2015.igem.org/Team:Amoy</a></p>
 
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<p style="font-size: 14px; color: #fff; margin-bottom: 0px;"><strong>Address:  </strong>Xiamen University, No. 422, Siming South Road, Xiamen, Fujian, P.R.China 361005</p>
 
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Latest revision as of 14:38, 10 September 2015

Attributions

Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster.

Can we base our project on a previous one?

Yes! You can have a project based on a previous team, or based on someone else's idea, as long as you state this fact very clearly and give credit for the original project.

Why is this page needed?

The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.

For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.

What should this page have?
  • General Support
  • Project support and advice
  • Fundraising help and advice
  • Lab support
  • Difficult technique support
  • Project advisor support
  • Wiki support
  • Presentation coaching
  • Human Practices support
  • Thanks and acknowledgements for all other people involved in helping make a successful iGEM team

Inspiration

Take a look at what other teams have done: