Difference between revisions of "Team:TCU Taiwan/Part"
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<td><a href="http://parts.igem.org/Part:BBa_K1727000">BBa_K1727000</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1727000">BBa_K1727000</a></td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| − | <td> | + | <td><a title="Signiferin is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. Signiferin has demonstrated effectiveness in killing Methicillin-Resistant Staphylococcus aureus (MRSA), and has been proven by the TU-Delft 2013 iGEM team. In order to have more efficient to get Signiferin, we treated signal peptide from S.lividans on the upstream of N-terminal of antimicrobial peptides to facilitate the peptide production." href="">Signiferin</a></td> |
<td>179 bp</td> | <td>179 bp</td> | ||
</tr> | </tr> | ||
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<td><a href="http://parts.igem.org/Part:BBa_K1727001">BBa_K1727001</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1727001">BBa_K1727001</a></td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| − | <td>Epinecidin-1</td> | + | <td><a title="Epinecidin-1 is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Epinephelus coioides to against microbes. Epinecidin-1 use it's chargeability to interact with bacteria cell membrane. Than use hydrophobic region interfere the membrane structure. This leads to cell lysis and bypasses bacterial antibiotic drug-resistance mechanisms. Moreover Epinecidin-1 has ability to help wound healing and has been proven by animal studies." href="">Epinecidin-1</a></td> |
<td>216 bp</td> | <td>216 bp</td> | ||
</tr> | </tr> | ||
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<td><a href="http://parts.igem.org/Part:BBa_K1727002">BBa_K1727002</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1727002">BBa_K1727002</a></td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| − | <td>Signal peptide</td> | + | <td><a title="This signal peptide is comes from the signal of chitinase C which extracted by S.lividans. This signal had been proved that can worked in E.coli and successfully secret target protein to E.coli LB culture medium. After translation process signal peptide will lead mature peptide to the secretion system of E.coli. When the pre-mature peptides go through the periplasmic space, peptidase will identified the cleavage site Ala-Gln-Ala and cut at the double Ala at the signal and mature peptide linkage site. Then separate signal peptide from mature peptide. Finally, secreting out mature peptide into the culture medium." href="">Signal peptide</a></td> |
<td>93 bp</td> | <td>93 bp</td> | ||
</tr> | </tr> | ||
| Line 90: | Line 90: | ||
<td width="119"><a href="http://parts.igem.org/Part:BBa_K1727003">BBa_K1727003</a></td> | <td width="119"><a href="http://parts.igem.org/Part:BBa_K1727003">BBa_K1727003</a></td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| − | <td width="185">Epinecidin-1 with signal peptide</td> | + | <td width="185"><a title="Epinecidin-1 is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Epinephelus coioides to against microbes. Moreover Epinecidin-1 has ability to help wound healing and has been proven by animal studies. In order to have more efficient to get Epinecidin-1, we treated signal peptide from S.lividans on the upstream of N-terminal of antimicrobial peptides to facilitate the peptide production." href="">Epinecidin-1 with signal peptide</a></td> |
<td width="218">309 bp</td> | <td width="218">309 bp</td> | ||
</tr> | </tr> | ||
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<td><a href="http://parts.igem.org/Part:BBa_K1727004">BBa_K1727004</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1727004">BBa_K1727004</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| − | <td><I>Lac</I> operator+RBS+signal peptide</td> | + | <td><a title="Epinecidin-1 is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Epinephelus coioides to against microbes. Moreover Epinecidin-1 has ability to help wound healing and has been proven by animal studies. In order to have more efficient to get Epinecidin-1, we treated signal peptide from S.lividans on the upstream of N-terminal of antimicrobial peptides to facilitate the peptide production. This part is built by part:BBa_K1727003.<br> |
| + | We used Lac operator, RBS, terminator on pQE 60 vector. So we can use IPTG induction to get our product." href=""><I>Lac</I> operator+RBS+signal peptide</a></td> | ||
<td>275 bp</td> | <td>275 bp</td> | ||
</tr> | </tr> | ||
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<td><a href="http://parts.igem.org/Part:BBa_K1727006">BBa_K1727006</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1727006">BBa_K1727006</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| − | <td><I>Lac</I> operator+RBS+Signal peptide+Signiferin<br>+Terminator</td> | + | <td><a title="Antimicrobial peptides (AMPs) are stable peptides that have extensive ability to kill or inhibit the growth of bacteria. They play a role in defense mechanism for all organisms, ranging from prokaryotes to humans, to against predators. Some of them act against specific strain of microbes. |
| + | Signiferin is a kinds of antimicrobial peptide extracted from the skin mucus of Crinia signifera, respectively. It demonstrated effectiveness in killing MRSA. And we had change it's sequence into E.coli codon usage. We treat a signal peptide in front of signiferin. This signal peptide comes from S.lividas and has ability to let E.coli secrect signiferin to LB culture medium.<br> | ||
| + | We used Lac operator,RBS,terminator on pQE60 to made our expression machine worked." href=""><I>Lac</I> operator+RBS+Signal peptide+Signiferin<br>+Terminator</a></td> | ||
<td>179 bp</td> | <td>179 bp</td> | ||
</tr> | </tr> | ||
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<td><a href="http://parts.igem.org/Part:BBa_K1727007">BBa_K1727007</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1727007">BBa_K1727007</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| − | <td>Promoter+<I>Lac</I> operator+RBS+signal peptide<br>+Epinecidin-1+Terminater</td> | + | <td><a title="Epinecidin-1 is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Epinephelus coioides to against microbes. Moreover Epinecidin-1 has ability to help wound healing and has been proven by animal studies. In order to have more efficient to get Epinecidin-1, we treated signal peptide from S.lividans on the upstream of N-terminal of antimicrobial peptides to facilitate the peptide production. This part is built by part:BBa_K1727003.<br> |
| + | We used T5 promoter, Lac operator, RBS, terminator on pQE 60 vector. So we can use IPTG induction to get our product." href="">Promoter+<I>Lac</I> operator+RBS+signal peptide<br>+Epinecidin-1+Terminater</a></td> | ||
<td>216 bp</td> | <td>216 bp</td> | ||
</tr> | </tr> | ||
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<td><a href="http://parts.igem.org/Part:BBa_K1727008">BBa_K1727008</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1727008">BBa_K1727008</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| − | <td>Promoter+<I>Lac</I> operator+RBS+Signal peptide<br>+Signiferin+Terminater</td> | + | <td><a title="Signiferin is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. Signiferin has demonstrated effectiveness in killing Methicillin-Resistant Staphylococcus aureus (MRSA), and has been proven by the TU-Delft 2013 iGEM team. In order to have more efficient to get Signiferin, we treated signal peptide from S.lividans on the upstream of N-terminal of antimicrobial peptides to facilitate the peptide production.<br> |
| + | This part is built by part:BBa_K1727000. We used T5 promoter, Lac operator, RBS, terminator on pQE 60 vector. So we can use IPTG induction to get our product." href="">Promoter+<I>Lac</I> operator+RBS+Signal peptide<br>+Signiferin+Terminater</a></td> | ||
<td>179 bp</td> | <td>179 bp</td> | ||
</tr> | </tr> | ||
Revision as of 14:39, 10 September 2015
This year TCU_Taiwan iGEM team had built new standard biological parts for iGEM community. Click on the name of the parts for detailed information.
Parts table
| Favorite | Name | Type | Description | Legth |
|---|---|---|---|---|
| BBa_K1727000 | Coding | Signiferin | 179 bp | |
| BBa_K1727001 | Coding | Epinecidin-1 | 216 bp | |
| BBa_K1727002 | Coding | Signal peptide | 93 bp | |
| BBa_K1727003 | Coding | Epinecidin-1 with signal peptide | 309 bp | |
| BBa_K1727004 | Composite | Lac operator+RBS+signal peptide | 275 bp | |
| BBa_K1727006 | Composite | Lac operator+RBS+Signal peptide+Signiferin +Terminator |
179 bp | |
| BBa_K1727007 | Composite | Promoter+Lac operator+RBS+signal peptide +Epinecidin-1+Terminater |
216 bp | |
| BBa_K1727008 | Composite | Promoter+Lac operator+RBS+Signal peptide +Signiferin+Terminater |
179 bp |
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| Contact us tcutaiwan@gmail.com No.701, Sec. 3, Zhongyang Rd. Hualien 97004, Taiwan |