Difference between revisions of "Team:TCU Taiwan/Part"

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           <td><a  href="http://parts.igem.org/Part:BBa_K1727004">BBa_K1727004</a></td>
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           <td><a  href="http://parts.igem.org/Part:BBa_K1727005">BBa_K1727005</a></td>
 
           <td>Composite</td>
 
           <td>Composite</td>
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           <td><a title="Epinecidin-1 is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Epinephelus coioides to against microbes. Moreover Epinecidin-1 has ability to help wound healing and has been proven by animal studies. In order to have more efficient to get Epinecidin-1, we treated signal peptide from S.lividans on the upstream of N-terminal of antimicrobial peptides to facilitate the peptide production. This part is built by part:BBa_K1727003.<br>
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           <td><a title="Signiferin is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Crinia signifera to against microbes. Signiferin use it's chargeability to interact with bacteria cell membrane. Than use hydrophobic region interfere the membrane structure. This leads to cell lysis and bypasses bacterial antibiotic drug-resistance mechanisms. Signiferin has demonstrated effectiveness in killing Methicillin-Resistant Staphylococcus aureus (MRSA), and has been proven by the TU-Delft 2013 iGEM team." href="">Signiferin (Crinia signifera)</a></td>
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We used Lac operator, RBS, terminator on pQE 60 vector. So we can use IPTG induction to get our product." href=""><I>Lac</I>&nbsp;operator+RBS+signal peptide</a></td>
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           <td>275 bp</td>
 
           <td>275 bp</td>
 
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          <th height="34" scope="30">&nbsp;</th>
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          <td><a  href="http://parts.igem.org/Part:BBa_K1727006">BBa_K1727006</a></td>
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          <td>Composite</td>
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          <td><a title="Antimicrobial peptides (AMPs) are stable peptides that have extensive ability to kill or inhibit the growth of bacteria. They play a role in defense mechanism for all organisms, ranging from prokaryotes to humans, to against predators. Some of them act against specific strain of microbes.
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Signiferin is a kinds of antimicrobial peptide extracted from the skin mucus of Crinia signifera, respectively. It demonstrated effectiveness in killing MRSA. And we had change it's sequence into E.coli codon usage. We treat a signal peptide in front of signiferin. This signal peptide comes from S.lividas and has ability to let E.coli secrect signiferin to LB culture medium.<br>
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We used Lac operator,RBS,terminator on pQE60 to made our expression machine worked." href=""><I>Lac</I>&nbsp;operator+RBS+Signal&nbsp;peptide+Signiferin<br>+Terminator</a></td>
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          <td>179 bp</td>
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Revision as of 14:44, 10 September 2015

This year TCU_Taiwan iGEM team had built new standard biological parts for iGEM community. Click on the name of the parts for detailed information.



             
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