Difference between revisions of "Team:UiOslo Norway/Entrepreneurship"

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<p></br></br></p>
 
<h1 style="font-size:300%"> Methane Incorporated </h1>
 
<p>(To hover over the picture to get the seperate subgoals.)</p>
 
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<p>
 
Methane  (CH<sub>4</sub>)  is  the  second  most  prevalent  emitted  greenhouse  gas  on  earth.
 
Today we are facing the challenge of solving this problem of high methane concentrations in the atmosphere that leads to global warming.  The  global  warming  potential  of  methane  is  about  84
 
times higher than that of carbon dioxide (CO<sub>2</sub>) when compared over 20 years. 
 
Technical methods to reuse emitted methane gas are cost- and time- intensive and therefore rarely used.
 
</p>
 
 
<p>
 
The goal of our  project is  to  develop an <i>Escherichia coli</i> (<i>E. coli</i>)
 
based  model  that filters methane out of the air and converts this into biomass.  First part of the project is to break down methane to methanol with the enzyme complex soluble  methane  monooxygenase (sMMO)  of  the
 
methanotroph <i>Methylococcus  capsulatus</i> expressed in <i>E.  coli</i>.  In  order  for  the  multisubunit
 
enzyme complex to break down methane to methanol, oxygen and iron-ions are required. Second
 
part of the project is to convert methanol into biomass using the Ribulose-Monophosphate (RuMP)- pathway and establish this pathway in  <i>E.coli.</i>  The  pathway  is  found  in  the  methanotroph <i>Bacillus  methanolicus</i> and enables
 
<i>E. coli</i> to convert methanol into biomass.
 
</p>
 
 
<p>
 
To test the functionality of the modified <i>E. coli</i>, the bacteria will be grown in a closed
 
system  under  methane  rich  conditions.  Additionally, a  filter  will be  created  that  can
 
contain the modified <i>E.coli</i> and can filter the surrounding air through the system, so that the methane can be broken down. The filter could be used in areas where methane emission is a problem.
 
</p>
 
 
 
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<div class="clear"></div>
 
<!---------------sponsors------------------------->
 
 
 
 
<center><h1>iGEM UiOslo 2015 was sponsored by:</h1>
 
<hr style="height:2px;border:none;color:black;background-color:black;" />
 
<img src="https://static.igem.org/mediawiki/2015/0/08/UiOslo_Enova1.jpg" Height="60px" hspace="15">
 
<img src="https://static.igem.org/mediawiki/2015/e/eb/UiOslo_GATC-Biotech.jpg" Height="60px" hspace="15">
 
<img src="https://static.igem.org/mediawiki/2015/4/4a/UiOslo_IDT.jpg" Height="60px" hspace="15">
 
<img src="https://static.igem.org/mediawiki/2015/d/d9/UiOslo_Norwegian_Biochemical_Society.jpg" Height="60px" hspace="15">
 
<img src="https://static.igem.org/mediawiki/2015/f/f0/UiOslo_SnapGene.jpg" Height="60px" hspace="15">
 
<img src="https://static.igem.org/mediawiki/2015/a/ac/UiOslo_Statoil.jpg" Height="60px" hspace="15">
 
</center>
 
<hr style="height:2px;border:none;color:black;background-color:black;" />
 
 
<!--------end sponsors---------------------------->
 
 
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Latest revision as of 20:57, 10 September 2015