Difference between revisions of "Team:Goettingen/Experiments"

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<a onClick=" $('#menu2').slideToggle(300, function callback() {  });"><h1>Plasmid Extraction - using peqGOLD Plasmid Miniprep Kit I (PEQLAB Technologies)</h1></a>
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     <a onClick=" $('#menu3').slideToggle(300, function callback() {  });"><h1>Plasmid Extraction - using QIAprep Spin Miniprep Kit (QIAGEN)</h1></a>
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<a onClick=" $('#menu4').slideToggle(300, function callback() {  });"><h1>Blunt End Ligation in pJET1.2 vector &ndash;Clone JET PCR Cloning Kit&ndash;Thermo Scientific</h1></a>
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<a href="" onClick=" $('#menu4').slideToggle(300, function callback() {  });"><h1>Blunt End Ligation in pJET1.2 vector &ndash;Clone JET PCR Cloning Kit&ndash;Thermo Scientific</h1></a>
 
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     <a onClick=" $('#menu5').slideToggle(300, function callback() {  });"><h1>TOPO&reg; Cloning protocol usingChampion&trade; pET Directional TOPO&reg; Expression Kits. Thermo Fisher Scientific</h1></a>
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     <a href="" onClick=" $('#menu5').slideToggle(300, function callback() {  });"><h1>TOPO&reg; Cloning protocol usingChampion&trade; pET Directional TOPO&reg; Expression Kits. Thermo Fisher Scientific</h1></a>
 
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         <a onClick=" $('#menu6').slideToggle(300, function callback() {  });"><h1>PCR product purification using QIAquick&reg; PCR Purification Kit (QIAGEN).</h1></a>
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             <a onClick=" $('#menu7').slideToggle(300, function callback() {  });"><h1>Plasmid transformation into <strong>Chemically Competent <em>E. coli</em></strong>.</h1></a>
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             <a href="" onClick=" $('#menu7').slideToggle(300, function callback() {  });"><h1>Plasmid transformation into <strong>Chemically Competent <em>E. coli</em></strong>.</h1></a>
 
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                 <a onClick=" $('#menu8').slideToggle(300, function callback() {  });"><h1>Preparation of competent <em>E.coli </em>cells</h1></a>
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                     <a onClick=" $('#menu9').slideToggle(300, function callback() {  });"><h1>Esterase activity test:</h1></a>
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     <p>Materials:</p>
 
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                         <a onClick=" $('#menu10').slideToggle(300, function callback() {  });"><h1>PCR Gel extraction, peqGOLD Gel Extraction Kit</h1></a>
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                             <a onClick=" $('#menu11').slideToggle(300, function callback() {  });"><h1>Electroporation of BL21 cells with pJET_RFP</h1></a>
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                                 <a onClick=" $('#menu12').slideToggle(300, function callback() {  });"><h1>Media preparation for cellulase activity screening</h1></a>
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Revision as of 22:59, 10 September 2015



LB Medium

Plasmid Extraction - using peqGOLD Plasmid Miniprep Kit I (PEQLAB Technologies)

Plasmid Extraction - using QIAprep Spin Miniprep Kit (QIAGEN)

Blunt End Ligation in pJET1.2 vector –Clone JET PCR Cloning Kit–Thermo Scientific

TOPO® Cloning protocol usingChampion™ pET Directional TOPO® Expression Kits. Thermo Fisher Scientific

PCR product purification using QIAquick® PCR Purification Kit (QIAGEN).

Plasmid transformation into Chemically Competent E. coli.

Preparation of competent E.coli cells

Esterase activity test:

PCR Gel extraction, peqGOLD Gel Extraction Kit

Electroporation of BL21 cells with pJET_RFP

Media preparation for cellulase activity screening

Experiments & Protocols

Describe the experiments, research and protocols you used in your iGEM project.

What should this page contain?
  • Protocols
  • Experiments
  • Documentation of the development of your project

Inspiration