Difference between revisions of "Team:Shiyan SY China/Parts"
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<p>© 2015 Shiyan_SY_China iGEM Team. All rights reserved. | <p>© 2015 Shiyan_SY_China iGEM Team. All rights reserved. | ||
Revision as of 04:02, 11 September 2015
You have to believe in yourself. That’s the secret of success.
—— Charles Chaplin
This part includes two individual DNA domains. 1. A strong promoter followed with a RNA thermometer sequence, which regulate the transcription of opdA gene and expression of opdA protein out of the host cell via an ompA peptide. This DNA fragment domain could be obtained by double-digested with EcoR I + Spe I. 2. A UV induced promoter (RecA SOS) followed with a suicide gene ccdB, which could lead to the death of host cell if exposed in UV. This DNA fragment domain could be obtained by double-digested with Xho I + Spe I. And the whole part DNA fragment could be obtained by double-digested with Xho I + EcoR I.
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1667005 | DNA | OpdA encoding gene with ompA signal peptide | 1776 |
© 2015 Shiyan_SY_China iGEM Team. All rights reserved.
