Difference between revisions of "Team:Stanford-Brown/InterLab Study"
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+ | <p><b>The three devices are:</b></p> | ||
+ | <p>Device 1: <a href="http://parts.igem.org/Part:BBa_J23101">J23101</a> + <a href="http://parts.igem.org/Part:BBa_I13504">I13504</a>, in <a href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></p> | ||
+ | <p>Device 2: <a href="http://parts.igem.org/Part:BBa_J23106">J23106</a> + <a href="http://parts.igem.org/Part:BBa_I13504">I13504</a>, in <a href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></p> | ||
+ | <p>Device 3: <a href="http://parts.igem.org/Part:BBa_J23117">J23117</a> + <a href="http://parts.igem.org/Part:BBa_I13504">I13504</a>, in <a href="http://parts.igem.org/Part:pSB1C3">pSB1C3</a></p> | ||
− | + | <p>For our positive control, we used the one suggested: <a href="http://parts.igem.org/Part:BBa_I20270">GFP device BBa_I20270</a>. For our negative control, we also used the one suggested: <a href="http://parts.igem.org/Part:BBa_R0040">TetR repressible promoter</a>.</p> | |
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+ | <p>All devices and parts were obtained from the 2015 Distribution Kit. Please see the link to our lab notebook below for more detailed information on device assembly. The results were confirmed with DNA sequencing by Elim Bio.</p> | ||
+ | <p>We also participated in the Extra Credit Opportunity. | ||
</p> | </p> | ||
Latest revision as of 05:13, 11 September 2015
InterLab Measurement Study 2015
Overview
How do standard promoters behave in Mountain View, California, USA? We participated in the 2nd Annual InterLab Measurement study to contribute our results to a body of data from teams from around the world!
See our BioBricksBackground
The purpose of the 2015 InterLab Study is to measure the fluorescence of three devices: a high-, medium-, and low-strength promoters fused to a green fluorescent protein (GFP) generator. iGEM teams from around the world construct the same devices and compare results.
The three devices are:
Device 1: J23101 + I13504, in pSB1C3
Device 2: J23106 + I13504, in pSB1C3
Device 3: J23117 + I13504, in pSB1C3
For our positive control, we used the one suggested: GFP device BBa_I20270. For our negative control, we also used the one suggested: TetR repressible promoter.
All devices and parts were obtained from the 2015 Distribution Kit. Please see the link to our lab notebook below for more detailed information on device assembly. The results were confirmed with DNA sequencing by Elim Bio.
We also participated in the Extra Credit Opportunity.
Experiment Engineering E. coli to produce polystyrene
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Donec tincidunt aliquet justo, sit amet mollis purus varius ac. Quisque ac sapien eu ante convallis cursus congue vel odio. Sed efficitur sapien ut eros sodales ornare. Vestibulum pellentesque lorem sed nulla interdum, non tincidunt velit sagittis. Vestibulum cursus, enim eu porta euismod, enim lectus facilisis diam, at sodales metus ligula sit amet eros. Sed ullamcorper, mauris nec mollis pretium, justo ligula dapibus nulla, non elementum nisl libero ut elit. Proin mi urna, finibus at scelerisque quis, porttitor at mauris. Nulla laoreet venenatis cursus. Vivamus et pellentesque quam, eget malesuada ex. Quisque eu massa ligula. Nam interdum dui sed laoreet efficitur. Aliquam sed vulputate orci. Pellentesque sed sollicitudin lectus. Vivamus nec tortor risus. Vestibulum malesuada feugiat lorem a dignissim. In diam mauris, venenatis at vulputate eget, venenatis sit amet metus. Suspendisse ut mi in ipsum sagittis malesuada at nec erat. Etiam volutpat risus quis nisi hendrerit porttitor vel eu tortor. Donec venenatis, risus sit amet ullamcorper scelerisque, tellus erat consequat nibh, vel dictum velit augue id leo. In eleifend tristique ipsum sed dignissim. Duis mattis, ipsum nec aliquet varius, turpis orci tempus nulla, in sodales libero massa at diam. Nulla maximus eros sed venenatis congue. Phasellus diam nunc, ullamcorper vitae tempor eget, sagittis eu odio. Praesent a mauris porttitor, mattis sem a, sodales massa. Proin et justo lectus. Proin varius magna ac leo ullamcorper accumsan. Proin id diam eget dolor vulputate mattis. Suspendisse pellentesque, nunc sit amet blandit feugiat, risus eros egestas massa, nec condimentum ante sapien ac velit. Vivamus efficitur justo dolor, at gravida lorem venenatis at. Aenean at ligula sapien. Mauris eget eleifend justo, eget faucibus ante. Ut mattis ante vitae dignissim maximus. Integer feugiat arcu purus, a viverra dui elementum vitae. Phasellus mattis porttitor iaculis. In eu nisi eu augue lacinia fringilla venenatis at nunc. Nam est erat, hendrerit ac dignissim sed, mollis eu eros. Morbi vel egestas dui, consectetur posuere nisi. Aliquam vitae tortor vulputate, fringilla est vel, faucibus diam. Suspendisse potenti. Donec sed commodo nulla. Duis feugiat, diam eu pulvinar rhoncus, arcu erat pretium orci, ut porta diam elit eu mi. Etiam eros massa, egestas eu mattis id, hendrerit at ligula. Duis placerat felis nec risus volutpat lobortis. Sed elementum, dolor non feugiat placerat, libero sapien pharetra diam, sed faucibus est ex tristique sem. Vivamus rutrum libero eget mollis sodales. Pellentesque vel scelerisque felis, a imperdiet erat. Fusce quis nisl magna. Sed non libero ultrices sapien hendrerit suscipit aliquet convallis leo. Quisque nec aliquam libero, in commodo ex. In eget nulla consequat, commodo quam id, hendrerit velit. Vestibulum non interdum enim. Ut elit justo, suscipit vel pretium vitae, rutrum sed dui. Donec vehicula sit amet ex ac finibus. Donec ultrices tellus et laoreet dictum.
Data and Results Optimizing the production of biological PHA
Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus commodo.
Donec tincidunt aliquet justo, sit amet mollis purus varius ac. Quisque ac sapien eu ante convallis cursus congue vel odio. Sed efficitur sapien ut eros sodales ornare. Vestibulum pellentesque lorem sed nulla interdum, non tincidunt velit sagittis. Vestibulum cursus, enim eu porta euismod, enim lectus facilisis diam, at sodales metus ligula sit amet eros. Sed ullamcorper, mauris nec mollis pretium, justo ligula dapibus nulla, non elementum nisl libero ut elit. Proin mi urna, finibus at scelerisque quis, porttitor at mauris. Nulla laoreet venenatis cursus. Vivamus et pellentesque quam, eget malesuada ex. Quisque eu massa ligula. Nam interdum dui sed laoreet efficitur. Aliquam sed vulputate orci. Pellentesque sed sollicitudin lectus. Vivamus nec tortor risus. Vestibulum malesuada feugiat lorem a dignissim. In diam mauris, venenatis at vulputate eget, venenatis sit amet metus. Suspendisse ut mi in ipsum sagittis malesuada at nec erat. Etiam volutpat risus quis nisi hendrerit porttitor vel eu tortor. Donec venenatis, risus sit amet ullamcorper scelerisque, tellus erat consequat nibh, vel dictum velit augue id leo. In eleifend tristique ipsum sed dignissim. Duis mattis, ipsum nec aliquet varius, turpis orci tempus nulla, in sodales libero massa at diam. Nulla maximus eros sed venenatis congue. Phasellus diam nunc, ullamcorper vitae tempor eget, sagittis eu odio. Praesent a mauris porttitor, mattis sem a, sodales massa. Proin et justo lectus. Proin varius magna ac leo ullamcorper accumsan. Proin id diam eget dolor vulputate mattis. Suspendisse pellentesque, nunc sit amet blandit feugiat, risus eros egestas massa, nec condimentum ante sapien ac velit. Vivamus efficitur justo dolor, at gravida lorem venenatis at. Aenean at ligula sapien. Mauris eget eleifend justo, eget faucibus ante. Ut mattis ante vitae dignissim maximus. Integer feugiat arcu purus, a viverra dui elementum vitae. Phasellus mattis porttitor iaculis. In eu nisi eu augue lacinia fringilla venenatis at nunc. Nam est erat, hendrerit ac dignissim sed, mollis eu eros. Morbi vel egestas dui, consectetur posuere nisi. Aliquam vitae tortor vulputate, fringilla est vel, faucibus diam. Suspendisse potenti. Donec sed commodo nulla. Duis feugiat, diam eu pulvinar rhoncus, arcu erat pretium orci, ut porta diam elit eu mi. Etiam eros massa, egestas eu mattis id, hendrerit at ligula. Duis placerat felis nec risus volutpat lobortis. Sed elementum, dolor non feugiat placerat, libero sapien pharetra diam, sed faucibus est ex tristique sem. Vivamus rutrum libero eget mollis sodales. Pellentesque vel scelerisque felis, a imperdiet erat. Fusce quis nisl magna. Sed non libero ultrices sapien hendrerit suscipit aliquet convallis leo. Quisque nec aliquam libero, in commodo ex. In eget nulla consequat, commodo quam id, hendrerit velit. Vestibulum non interdum enim. Ut elit justo, suscipit vel pretium vitae, rutrum sed dui. Donec vehicula sit amet ex ac finibus. Donec ultrices tellus et laoreet dictum.
See our Picture Gallery!Protocols
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Nam sollicitudin enim ac egestas fermentum. Suspendisse tempor urna vel mollis mollis. Proin ac mauris facilisis sapien maximus suscipit nec eget felis. Fusce ac urna sit amet nunc condimentum gravida. Aenean commodo nunc et tempus egestas. Suspendisse cursus quam placerat, vestibulum nunc non, imperdiet felis. Curabitur et erat non justo eleifend commodo. In sit amet sem vitae eros placerat facilisis. Quisque eget ligula vel tellus fermentum vestibulum. Curabitur eu ligula non lorem pulvinar posuere ac commodo ante. Sed convallis quam ut risus dignissim, nec pellentesque risus malesuada. Vestibulum vel sem eu tortor ornare consequat ac eget ligula. Suspendisse eu lacus ut nisi aliquet mollis id nec eros. Integer vulputate sem sed massa porta, eget dapibus odio pellentesque. Morbi sit amet lacus quis urna mattis elementum. See our Lab Notebook!References
[1] Anderson, A. J. and Dawes, E. A. Occurrence, Metabolism, Metabolic Role, and Industrial Uses of Bacterial Polyhydroxyalkanoates. Microbiological Reviews 54 (4), 1990. 450 – 472.
[2] Madison, L. L. and Huisman, G. W. Metabolic Engineering of Poly(3-Hydroxyalkanoates): From DNA to Plastic. Microbiol Mol Biol Rev 63 (1), 1999. 21 – 53.
[3] Kunasundari, B. and Sudesh, K. Isolation and recovery of microbial polyhydroxyalkanoates. eXPRESS Polymer Letters 5 (7), 2011. 620 – 634.
[4] Leonardi, R. et al. Coenzyme A: Back in action. Progress in Lipid Research 44, 2005. 125 – 153.
[5] Leonardi, R. et al. A Pantothenate Kinase from Staphylococcus aureus Refractory to Feedback Regulation by Coenzyme A. The Journal of Biological Chemistry 280, 2005. 3312 – 3322.
[6] Jacquel, N. et al. Isolation and purification of bacterial poly(3-hydroxyalkanoates). Biochemical Engineering Journal 39 (1), 2008. 15 – 27.
[7] Rahman, A. et al. Secretion of polyhydroxybuterate in Escherichia coli using a synthetic biological engineering approach. Journal of Biological Engineering 7 (24), 2013.