Difference between revisions of "Team:Austin UTexas"

Revision as of 20:14, 11 September 2015

UT Austin iGEM 2015 Home

BREAKING IS BAD

Our iGEM team, under the supervision of the Barrick lab at the University of Texas at Austin, developed five individual projects inspired by members’ interests and concerns in synthetic biology, with a foundation of technical skills and lab experience built during a spring semester course. The projects our team members have devised focus on a multitude of topics, from attempts at improving the stability and efficiency of existing genetic machines, to identifying bacterial factories that can have ecological function. Our projects focused on improving and expanding on the existing microbial factories in E. coli include an attempt to optimize the ΔguaB pDCAF strain of E. coli (Quandt et al., 2013) to discount nutrients provided by non-caffeine methylxanthines, and a project assessing the evolutionary stability of yellow fluorescent protein, enhanced yellow fluorescent protein, and super-folder yellow fluorescent protein.

References

Quandt, Erik M., et al. "Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5." ACS synthetic biology 2.6 (2013): 301-307.

Zhang, Hui-Juan, et al. "Biotransformation of the neonicotinoid insecticide thiacloprid by the bacterium Variovorax boronicumulans strain J1 and mediation of the major metabolic pathway by nitrile hydratase."Journal of agricultural and food chemistry 60.1 (2011): 153-159.

Liu, Juan, et al. "An improved method for extracting bacteria from soil for high molecular weight DNA recovery and BAC library construction." The Journal of Microbiology 48.6 (2010): 728-733.

GO TO PROJECT PAGE

CAFFEINATED COLI

redesign for greater stability

GO TO CAFFEINE PAGE

INTERLAB STUDY & BREAKING IS BAD

what does BB have to do with interlab?

GO TO INTERLAB STUDY PAGE

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-<p><h3><font face="Courier New">BREAKING IS BAD</font></h3></p>
+<h3><font face="Courier New">BREAKING IS BAD</font></h3>
+<br>
+<font color="000000">Our iGEM team, under the supervision of the Barrick lab at the University of Texas at Austin, developed five individual projects inspired by members’ interests and concerns in synthetic biology, with a foundation of technical skills and lab experience built during a spring semester course. The projects our team members have devised focus on a multitude of topics, from attempts at improving the stability and efficiency of existing genetic machines, to identifying bacterial factories that can have ecological function. Our projects focused on improving and expanding on the existing microbial factories in E. coli include an attempt to optimize the ΔguaB pDCAF strain of <i>E. coli</i> (Quandt <i>et al.</i>, 2013) to discount nutrients provided by non-caffeine methylxanthines, and a project assessing the evolutionary stability of yellow fluorescent protein, enhanced yellow fluorescent protein, and super-folder yellow fluorescent protein. </font><br>
+<br>
+<img src="https://static.igem.org/mediawiki/2015/1/16/2015_Austin_UTexas_PLACEHOLDERFIGURE.png" align="left" hspace="25px">
+<br>
 <br>
-<font color="000000">Our iGEM team, under the supervision of the Barrick lab at the University of Texas at Austin, developed five individual projects inspired by members’ interests and concerns in synthetic biology, with a foundation of technical skills and lab experience built during a spring semester course. The projects our team members have devised focus on a multitude of topics, from attempts at improving the stability and efficiency of existing genetic machines, to identifying bacterial factories that can have ecological function. Our projects focused on improving and expanding on the existing microbial factories in E. coli include an attempt to optimize the ΔguaB pDCAF strain of <i>E. coli</i> (Quandt <i>et al.</i>, 2013) to discount nutrients provided by non-caffeine methylxanthines, and a project assessing the evolutionary stability of yellow fluorescent protein, enhanced yellow fluorescent protein, and super-folder yellow fluorescent protein. </font></br>
-</p><p>
 <b>References</b></br>
-<h6><p>Quandt, Erik M., et al. "Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5." ACS synthetic biology 2.6 (2013): 301-307.</br></h6>
+<h6>Quandt, Erik M., et al. "Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5." ACS synthetic biology 2.6 (2013): 301-307.</br></h6>
-<p><h6>Zhang, Hui-Juan, et al. "Biotransformation of the neonicotinoid insecticide thiacloprid by the bacterium Variovorax boronicumulans strain J1 and mediation of the major metabolic pathway by nitrile hydratase."Journal of agricultural and food chemistry 60.1 (2011): 153-159.</br></h6>
+<h6>Zhang, Hui-Juan, et al. "Biotransformation of the neonicotinoid insecticide thiacloprid by the bacterium Variovorax boronicumulans strain J1 and mediation of the major metabolic pathway by nitrile hydratase."Journal of agricultural and food chemistry 60.1 (2011): 153-159.</br></h6>
-<p><h6>Liu, Juan, et al. "An improved method for extracting bacteria from soil for high molecular weight DNA recovery and BAC library construction." The Journal of Microbiology 48.6 (2010): 728-733.</br></h6></p><p></p>
+<h6>Liu, Juan, et al. "An improved method for extracting bacteria from soil for high molecular weight DNA recovery and BAC library construction." The Journal of Microbiology 48.6 (2010): 728-733.</br></h6></p><p></p>
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 <a href="https://2015.igem.org/Team:Austin_UTexas/Project/Problem"><font color="017e70"><b>GO TO PROJECT PAGE</b></font></a>