Difference between revisions of "Team:NTU-LIHPAO-Taiwan/Notebook"

Line 67: Line 67:
  
 
body {
 
body {
−
background-color: #F0F0F0;
+
background-color: #FFF;
 
}
 
}
 
body,td,th {
 
body,td,th {
Line 73: Line 73:
 
font-family: Calibri, "Arial Narrow", "Agency FB", "Raavi";
 
font-family: Calibri, "Arial Narrow", "Agency FB", "Raavi";
 
}
 
}
 +
 
#Wrapper {
 
#Wrapper {
 
margin-right: auto;
 
margin-right: auto;
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margin-bottom: 0px;
 
margin-bottom: 0px;
 
width: 90%;
 
width: 90%;
−
height: 10000px;
+
height: auto;
 
top: 0px;
 
top: 0px;
 
left: 5%;
 
left: 5%;
 
right: 5%;
 
right: 5%;
 
position: absolute;
 
position: absolute;
 +
bottom: 0px;
 +
padding-top: 0px;
 +
padding-bottom: 0px;
 +
z-index: 0;
 +
}
 +
 +
#Wrapper_Left {
 +
margin-right: auto;
 +
margin-left: auto;
 +
background-color: #F0F0F0;
 +
margin-top: 0px;
 +
margin-bottom: 0px;
 +
width: 5%;
 +
height: 100%;
 +
top: 0px;
 +
left: 0%;
 +
right: auto;
 +
position: fixed;
 +
bottom: 0px;
 +
padding-top: 0px;
 +
padding-bottom: 0px;
 +
z-index: 0;
 +
}
 +
 +
#Wrapper_Right {
 +
margin-right: auto;
 +
margin-left: auto;
 +
background-color: #F0F0F0;
 +
margin-top: 0px;
 +
margin-bottom: 0px;
 +
width: 5%;
 +
height: 100%;
 +
top: 0px;
 +
left: auto;
 +
right: 0%;
 +
position: fixed;
 
bottom: 0px;
 
bottom: 0px;
 
padding-top: 0px;
 
padding-top: 0px;
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height: 58px;
 
height: 58px;
 
top: 0px;
 
top: 0px;
−
left:0px;
+
left: 0px;
−
right:0px;
+
right: 0px;
 
position: fixed;
 
position: fixed;
 
bottom: 0px;
 
bottom: 0px;
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}
 
}
  
−
 
−
 
−
/*-------------------------------------------*/
 
−
/*---------------Bottom icons----------------*/
 
−
/*-------------------------------------------*/
 
−
 
−
#NTUschool_logo {
 
−
height: 70px;
 
−
width: 265px;
 
−
position: absolute;
 
−
left: 25%;
 
−
top: 5px;
 
−
right: auto;
 
−
bottom: auto;
 
−
z-index: 9999;
 
−
margin-left: -132px;
 
−
}
 
−
 
−
#LIHPAO_logo {
 
−
height: 70px;
 
−
width: 215px;
 
−
position: absolute;
 
−
left: 75%;
 
−
top: 5px;
 
−
right: auto;
 
−
bottom: auto;
 
−
z-index: 9999;
 
−
margin-left: -107px;
 
−
}
 
−
 
−
#NTUBST_logo {
 
−
height: 70px;
 
−
width: 70px;
 
−
position: absolute;
 
−
left: 50%;
 
−
top: 5px;
 
−
right: auto;
 
−
bottom: auto;
 
−
z-index: 9999;
 
−
margin-left: -35px;
 
−
}
 
−
 
−
#Bottom_Text {
 
−
color: #767676;
 
−
bottom: 0px;
 
−
width: 100%;
 
−
height: auto;
 
−
font-size: 15px;
 
−
position: absolute;
 
−
text-align: center;
 
−
}
 
  
  
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position: absolute;
 
position: absolute;
 
top: 5rem;
 
top: 5rem;
−
left: 2rem;
+
left: 10%;
 +
width: 90%;
 
}
 
}
  
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overflow-y: auto;
 
overflow-y: auto;
 
transition: 0.5s;
 
transition: 0.5s;
−
width: 10rem;
+
width: 85%;
−
padding-left: 2rem;
+
left: 15%;
−
padding-right: 1rem;
+
position: relative;
 
}
 
}
  
 
.sub-Content > li {
 
.sub-Content > li {
−
padding-top: 8px;
+
padding-top: 10px;
−
padding-bottom: 8px;
+
padding-bottom: 10px;
 
}
 
}
  
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#Healthin_Logo {
 
#Healthin_Logo {
−
top: 45px;
+
top: 55px;
 
padding-left: 3%;
 
padding-left: 3%;
 
padding-right: 3%;
 
padding-right: 3%;
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width: 94%;
 
width: 94%;
 
height: 94%;
 
height: 94%;
 +
}
 +
 +
 +
/*-------------------------------------------*/
 +
/*---------------Introduction----------------*/
 +
/*-------------------------------------------*/
 +
 +
.Container_Bottom1 {
 +
height: 70px;
 +
width: 100%;
 +
}
 +
 +
.Container_Bottom2 {
 +
height: 70px;
 +
width: 100%;
 +
padding-top: 10px;
 +
border-top-style: solid;
 +
border-top-color: #079235;
 +
border-top-width: 3px;
 +
}
 +
 +
.Intro_Picture {
 +
width: 100%;
 +
height: auto;
 +
background-color: #FFF;
 +
position: absolute;
 +
}
 +
 +
#NTUschool_logo {
 +
background-color: #FFF;
 +
padding-left: 9.6%;
 +
padding-right: 60.4%;
 +
position: absolute;
 +
width: 30%;
 +
height: 100%;
 +
}
 +
 +
#LIHPAO_logo {
 +
background-color: #FFF;
 +
padding-left: 66.4%;
 +
padding-right: 9.6%;
 +
position: absolute;
 +
width: 24%;
 +
height: 100%;
 +
}
 +
 +
#NTUBST_logo {
 +
background-color: #FFF;
 +
padding-left: 49.2%;
 +
padding-right: 43.2%;
 +
position: absolute;
 +
width: 7.6%;
 +
height: 100%;
 +
}
 +
 +
.Text_Sponsor {
 +
color: #767676;
 +
font-size: 15px;
 +
font-family: Calibri;
 +
text-align: center;
 +
line-height: 20px;
 
}
 
}
  
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Super {
 
Super {
 
vertical-align:super;
 
vertical-align:super;
 +
}/*-------------------------------------------*/
 +
/*-------------------Media-------------------*/
 +
/*-------------------------------------------*/
 +
 +
@media screen and (min-width: 0px) and (max-width: 1200px) {
 +
 +
#NTUXLIHPAO_icon {
 +
height: 40px;
 +
width: 200px;
 +
position: absolute;
 +
left: 1%;
 +
top: 18px;
 +
right: auto;
 +
bottom: auto;
 +
z-index: 9999;
 +
}
 +
 +
 +
#width_small {
 +
width: 95px; /*--改主要選單寬度--*/
 +
}
 +
 +
.Slidemenu {
 +
z-index: 9999;
 +
position: absolute;
 +
top: 32px;
 +
bottom: 0px;
 +
left: 49%;
 +
right: auto;
 +
list-style:none;
 +
margin:0;
 +
padding:0;
 +
position: absolute;
 +
margin-left: -320px;
 +
/*background:rgba(100%,100%,100%,0); 主選單色系選擇*/
 +
}
 
}
 
}
 +
 +
@media screen and (min-width: 901px) and (max-width: 1050px) {
 +
 +
.Container_Bottom2 {
 +
height: 60px;
 +
width: 100%;
 +
padding-top: 10px;
 +
border-top-style: solid;
 +
border-top-color: #079235;
 +
border-top-width: 3px;
 +
}
 +
}
 +
 +
@media screen and (min-width: 0px) and (max-width: 900px) {
 +
 +
.Container_Bottom2 {
 +
height: 50px;
 +
width: 100%;
 +
padding-top: 10px;
 +
border-top-style: solid;
 +
border-top-color: #079235;
 +
border-top-width: 3px;
 +
}
 +
}
 +
  
 
</style>
 
</style>
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<body>
 
<body>
 +
 +
<div id="Wrapper_Left"></div>
 +
<div id="Wrapper_Right"></div>
  
 
<div id="Wrapper">
 
<div id="Wrapper">
Line 955: Line 1,067:
 
</div>
 
</div>
  
 +
 +
 +
 +
 +
<div class="Container_Bottom1"></div>
 +
<div class="Container_Bottom2">
 +
<div class="Intro_Picture">
 +
<div id="NTUschool_logo"><a href="http://www.ntu.edu.tw/english/index.html"><img src="https://static.igem.org/mediawiki/2015/d/de/NTUschool_icon.png"  style="max-width: 100%; max-height: 70px"/></a></div>
 +
<div id="LIHPAO_logo"><a href="http://www.llsc.com.tw/"><img src="https://static.igem.org/mediawiki/2015/d/d4/LIHPAO_logo.png" style="max-width: 100%; max-height: 70px"/></a></div>
 +
<div id="NTUBST_logo"><a href="http://www.bst.ntu.edu.tw/BST-new/NTUBST.html"><img src="https://static.igem.org/mediawiki/2015/6/68/NTUBST_logo.png" style="max-width: 100%; max-height: 70px"/></a></div>
 +
</div>
 +
</div>
 +
<div class="Text_Sponsor">
 +
Maintained by the iGEM team NTU-LIHPAO-Taiwan&nbsp;&nbsp;&nbsp;&nbsp;©2015 NTU-LIHPAO-Taiwan
 +
</div>
  
  
Line 1,005: Line 1,132:
 
});
 
});
 
</script>
 
</script>
−
 
−
<div id="Wrapper_Bottom">
 
−
<div id="NTUschool_logo"><a href="http://www.ntu.edu.tw/english/index.html"><img src="https://static.igem.org/mediawiki/2015/d/de/NTUschool_icon.png" height="70" /></a></div>
 
−
<div id="LIHPAO_logo"><a href="http://www.llsc.com.tw/"><img src="https://static.igem.org/mediawiki/2015/d/d4/LIHPAO_logo.png" height="70" /></a></div>
 
−
<div id="NTUBST_logo"><a href="http://www.bst.ntu.edu.tw/BST-new/NTUBST.html"><img src="https://static.igem.org/mediawiki/2015/6/68/NTUBST_logo.png" height="70" /></a></div>
 
−
<div id="Bottom_Text">
 
−
Maintained by the iGEM team NTU-LIHPAO-Taiwan&nbsp;&nbsp;&nbsp;&nbsp;©2015 NTU-LIHPAO-Taiwan
 
−
</div>
 
−
</div>
 
 
</div>
 
</div>
  
 
</body>
 
</body>
 
</html>
 
</html>

Revision as of 06:10, 12 September 2015

NTU-LIHPAO-Taiwan

July
7/4
Manufacture CP+ plate
7/6
Learn how to transform
Apoptosis part:
Transformation of:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
7/7
Learn how to manufacture competent cell
Manufacture competent cell
PYY part:
Transformation of:
[promoter] (BBa_J23100), [signal peptide] (BBa_K554002), [terminator] (BBa_B0015)
Apoptosis part:
Transformation of:
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806), [C1] (BBa_C0051)
7/8
Learn how to extract the plasmid
Learn how to conduct the gel extraction and electrophoresis
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
Restriction enzyme:
[RBS] cleave by EcoRI, [C2] cleave by EcoRI and PstI
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C2] ----- Result: Succeeded
Transformation of:
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806) again
7/9
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C1] (BBa_C0051), [endolysin] (BBa_K112806)
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C1] ----- Result: Succeeded
[endolysin] ----- Result: Failed (Smeared band was too blurred)
PYY part:
Extract the plasmid:
[signal peptide] (BBa_K554002), [terminator] (BBa_B0015)
Gel electrophoresis:
[signal peptide] ----- Result: Succeeded
[terminator] ----- Result: Succeeded
7/10
Manufacture CP+ plate
Manufacture Amp+ plate
Apoptosis part:
Gel electrophoresis:
[RBS] (BBa_B0034) ----- Result: Failed (Smeared band was too blurred)
[C2] (BBa_C0053) ----- Result: Succeeded
Gel extraction:
[C2]
7/11
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C1] (BBa_C0051), [endolysin] (BBa_K112806)
PYY part:
Transformation of:
[promoter] (BBa_J23101)
7/12
Apoptosis part:
Extract the plasmid:
[pC1] (BBa_R0051)
7/13
PYY part:
Extract the plasmid:
[promoter] (BBa_J23101)
Gel electrophoresis (two white colonies & two red colonies):
[promoter] ------ Result: Failed (white colonies didn’t have band, red colonies had band but not the correct size, we speculated that was pollution)
7/20
Discuss about total synthesis
7/13
PYY part:
Transformation of:
[terminator] (BBa_B0015)
7/24
Discuss our project with professor
PYY part:
Extract the plasmid:
[terminator] (BBa_B0015)
Gel electrophoresis:
[terminator] ----- Result: Failed
7/25
Discuss our problems with Instructor
7/26
PYY part:
Cultured [terminator] again
7/27
PYY part:
Extract the plasmid:
[terminator] (BBa_B0015)
Gel electrophoresis:
[terminator] ----- Result: Succeeded
Maintained by the iGEM team NTU-LIHPAO-Taiwan    ©2015 NTU-LIHPAO-Taiwan