Difference between revisions of "Team:NTU-LIHPAO-Taiwan/Notebook"

Line 227: Line 227:
 
top: 32px;
 
top: 32px;
 
bottom: 0px;
 
bottom: 0px;
−
left: 50%;
+
left: 51%;
 
right: 15%;
 
right: 15%;
 
list-style:none;
 
list-style:none;
Line 308: Line 308:
 
}
 
}
  
−
#width_small {
+
.width_small {
 
width: 100px; /*--改主要選單寬度--*/
 
width: 100px; /*--改主要選單寬度--*/
−
}
 
−
 
−
#width_large {
 
−
width: 140px; /*--改主要選單寬度--*/
 
 
}
 
}
  
Line 401: Line 397:
 
padding-top: 5px;
 
padding-top: 5px;
 
padding-bottom: 5px;
 
padding-bottom: 5px;
 +
line-height: 30px;
 
}
 
}
  
Line 441: Line 438:
 
height: 94%;
 
height: 94%;
 
}
 
}
 +
  
  
Line 459: Line 457:
 
border-top-color: #079235;
 
border-top-color: #079235;
 
border-top-width: 3px;
 
border-top-width: 3px;
−
}
 
−
 
−
.Container_Top1{
 
−
height: 30px;
 
−
width: 100%;
 
 
}
 
}
  
Line 506: Line 499:
 
text-align: center;
 
text-align: center;
 
line-height: 20px;
 
line-height: 20px;
 +
}
 +
 +
 +
/*-------------------------------------------*/
 +
/*---------------Article Picture-------------*/
 +
/*-------------------------------------------*/
 +
 +
.Container_Article_Picture {
 +
height: 280px;
 +
width: 100%;
 +
padding-top: 25px;
 +
padding-bottom: 25px;
 +
}
 +
 +
.Article_Picture {
 +
width: 480px;
 +
height: auto;
 +
left: 50%;
 +
right: auto;
 +
background-color: #FFF;
 +
margin: 0 0 0 -240px;
 +
position: absolute;
 +
}
 +
 +
.Article_PictureText {
 +
width: 430px;
 +
height: auto;
 +
padding-left: 25px;
 +
padding-right: 25px;
 +
border-top-width: 2px;
 +
border-top-style: dashed;
 +
border-top-color: #fe5838;
 +
}
 +
 +
.Text_Picture {
 +
font-size: 18px;
 +
font-family: Calibri;
 +
text-align: justify;
 +
line-height: 26px;
 +
color: #08923a;
 
}
 
}
  
Line 705: Line 738:
 
<div class="Slidemenu">
 
<div class="Slidemenu">
 
<ul>
 
<ul>
−
<li><div id=width_small><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan">Home</a></div>
+
<li><div class=width_small><div id=Position_Now><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan">Home</a></div></div>
 
</li>
 
</li>
−
+
 
−
<li><div id=width_small><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Team">Team</a></div>
+
<li><div class=width_small span style="cursor:default"><a>Team</a></div>
−
</li>
+
−
+
−
<li><div id=width_small span style="cursor:default"><a>Project</a></div>
+
 
<ul class="subs">
 
<ul class="subs">
−
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Project/Abstract">Abstract</a></li>
+
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Team">Team</a></li>
−
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Project/Overview">Overview</a></li>
+
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Attributions">Attributions</a></li>
−
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Project/Parts">Parts</a></li>
+
−
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Project/Results">Results</a></li>
+
 
</ul>
 
</ul>
 
</li>
 
</li>
 
 
−
<li><div id=width_small span style="cursor:default"><a>Modeling</a></div>
+
<li><div class=width_small span style="cursor:default"><a>Project</a></div>
 
<ul class="subs">
 
<ul class="subs">
−
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Modeling/Abstract">Abstract</a></li>
+
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Description">Description</a></li>
−
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Modeling/Simulation">Simulation</a></li>
+
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Design">Design</a></li>
−
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Modeling/Results">Results</a></li>
+
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Results">Results</a></li>
 +
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Modeling">Modeling</a></li>
 +
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Experiments">Protocols</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>
 
 
−
<li><div id=width_small span style="cursor:default"><div id=Position_Now><a>Notebook</a></div></div>
+
<li><div class=width_small span style="cursor:default"><a>Parts</a></div>
 
<ul class="subs">
 
<ul class="subs">
−
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Notebook">Note</a></li>
+
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Parts">Team Parts</a></li>
−
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Protocols">Protocols</a></li>
+
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Basic_Part">Basic Parts</a></li>
 +
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Composite_Part">Composite Parts</a></li>
 +
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Part_Collection">Part Collection</a></li>
 
</ul>
 
</ul>
 +
</li>
 +
 +
<li><div class=width_small><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Notebook">Notebook</a></div>
 
</li>
 
</li>
 
          
 
          
−
<li><div id=width_small><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Safety">Safety</a></div>
+
<li><div class=width_small><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Safety">Safety</a></div>
 
</li>
 
</li>
 
 
−
<li><div id=width_large><a href="#">Human Practice</a></div>
+
<li><div class=width_small span style="cursor:default"><a>Society</a></div>
 
<ul class="subs">
 
<ul class="subs">
−
<li><a href="#">Sub Item</a></li>
+
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Practices">Human Practices</a></li>
−
<li><a href="#">Sub Item</a></li>
+
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Collaborations">Collaborations</a></li>
−
<li><a href="#">Sub Item</a></li>
+
<li><a href="https://2015.igem.org/Team:NTU-LIHPAO-Taiwan/Entrepreneurship">Entrepreneurship</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>

Revision as of 11:33, 12 September 2015

NTU-LIHPAO-Taiwan

7/4
Manufacture CP+ plate
7/6
Learn how to transform
Apoptosis part:
Transformation of:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
7/7
Learn how to manufacture competent cell
Manufacture competent cell
PYY part:
Transformation of:
[promoter] (BBa_J23100), [signal peptide] (BBa_K554002), [terminator] (BBa_B0015)
Apoptosis part:
Transformation of:
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806), [C1] (BBa_C0051)
7/8
Learn how to extract the plasmid
Learn how to conduct the gel extraction and electrophoresis
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
Restriction enzyme:
[RBS] cleave by EcoRI, [C2] cleave by EcoRI and PstI
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C2] ----- Result: Succeeded
Transformation of:
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806) again
7/9
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C1] (BBa_C0051), [endolysin] (BBa_K112806)
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C1] ----- Result: Succeeded
[endolysin] ----- Result: Failed (Smeared band was too blurred)
PYY part:
Extract the plasmid:
[signal peptide] (BBa_K554002), [terminator] (BBa_B0015)
Gel electrophoresis:
[signal peptide] ----- Result: Succeeded
[terminator] ----- Result: Succeeded
7/10
Manufacture CP+ plate
Manufacture Amp+ plate
Apoptosis part:
Gel electrophoresis:
[RBS] (BBa_B0034) ----- Result: Failed (Smeared band was too blurred)
[C2] (BBa_C0053) ----- Result: Succeeded
Gel extraction:
[C2]
7/11
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C1] (BBa_C0051), [endolysin] (BBa_K112806)
PYY part:
Transformation of:
[promoter] (BBa_J23101)
7/12
Apoptosis part:
Extract the plasmid:
[pC1] (BBa_R0051)
7/13
PYY part:
Extract the plasmid:
[promoter] (BBa_J23101)
Gel electrophoresis (two white colonies & two red colonies):
[promoter] ------ Result: Failed (white colonies didn’t have band, red colonies had band but not the correct size, we speculated that was pollution)
7/20
Discuss about total synthesis
7/13
PYY part:
Transformation of:
[terminator] (BBa_B0015)
7/24
Discuss our project with professor
PYY part:
Extract the plasmid:
[terminator] (BBa_B0015)
Gel electrophoresis:
[terminator] ----- Result: Failed
7/25
Discuss our problems with Instructor
7/26
PYY part:
Cultured [terminator] again
7/27
PYY part:
Extract the plasmid:
[terminator] (BBa_B0015)
Gel electrophoresis:
[terminator] ----- Result: Succeeded
Maintained by the iGEM team NTU-LIHPAO-Taiwan    ©2015 NTU-LIHPAO-Taiwan