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− | To control the AMPs expression and secretion, the <I>Lac</I> operon was used and treated signal peptide into our system. Helping peptides secret into culture medium. <a href="#tcu_references_5">[5, 6]</a> After purification of the peptide we will be testing the effectiveness of our synthetic AMPs. We will test macro-dilution of MRSA and in vitro wound healing assay for epithelial cells line (HaCaT) and micro vascular endothelial cells (HMEC-1). Next, application to the mouse model was conducted in vivo. Out goal is to create a wound dressing that is effective in inhibiting bacterial growth and assisting wound healing process. | + | To control the AMPs expression and secretion, the <I>Lac</I> operon was used and treated signal peptide into our system. Helping peptides secret into culture medium. <a href="#tcu_references_5">[5, 6]</a> After purification of the peptide we will be testing the effectiveness of our synthetic AMPs. We will test macro-dilution of MRSA and in vitro wound healing assay for epithelial cells line (HaCaT) and micro vascular endothelial cells (HMEC-1). Out goal is to create a wound dressing that is effective in inhibiting bacterial growth and assisting wound healing process. |
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