Difference between revisions of "Team:TCU Taiwan/Composite Part"

Line 76: Line 76:
 
           <td><a  href="http://parts.igem.org/Part:BBa_K1727001">BBa_K1727001</a></td>
 
           <td><a  href="http://parts.igem.org/Part:BBa_K1727001">BBa_K1727001</a></td>
 
           <td>Coding</td>
 
           <td>Coding</td>
−
           <td><a title="Epinecidin-1 is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Epinephelus coioides to against microbes. Epinecidin-1 use it's chargeability to interact with bacteria cell membrane. Than use hydrophobic region interfere the membrane structure. This leads to cell lysis and bypasses bacterial antibiotic drug-resistance mechanisms. Moreover Epinecidin-1 has ability to help wound healing and has been proven by animal studies." href="">Epinecidin-1</a></td>
+
           <td><a title="Epinecidin-1 is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Epinephelus coioides to against microbes. Epinecidin-1 use it's chargeability to interact with bacteria cell membrane. Than use hydrophobic region interfere the membrane structure. This leads to cell lysis and bypasses bacterial antibiotic drug-resistance mechanisms. Moreover Epinecidin-1 has ability to help wound healing and has been proven by animal studies." href="">Epinecidin-1 (<I>Epinephelus coioides</I>)</a></td>
 
           <td>216 bp</td>
 
           <td>216 bp</td>
 
         </tr>
 
         </tr>
Line 83: Line 83:
 
           <td><a  href="http://parts.igem.org/Part:BBa_K1727002">BBa_K1727002</a></td>
 
           <td><a  href="http://parts.igem.org/Part:BBa_K1727002">BBa_K1727002</a></td>
 
           <td>Coding</td>
 
           <td>Coding</td>
−
           <td><a title="This signal peptide is comes from the signal of chitinase C which extracted by S.lividans. This signal had been proved that can worked in E.coli and successfully secret target protein to E.coli LB culture medium. After translation process signal peptide will lead mature peptide to the secretion system of E.coli. When the pre-mature peptides go through the periplasmic space, peptidase will identified the cleavage site Ala-Gln-Ala and cut at the double Ala at the signal and mature peptide linkage site. Then separate signal peptide from mature peptide. Finally, secreting out mature peptide into the culture medium." href="">Signal peptide</a></td>
+
           <td><a title="This signal peptide is comes from the signal of chitinase C which extracted by S.lividans. This signal had been proved that can worked in E.coli and successfully secret target protein to E.coli LB culture medium. After translation process signal peptide will lead mature peptide to the secretion system of E.coli. When the pre-mature peptides go through the periplasmic space, peptidase will identified the cleavage site Ala-Gln-Ala and cut at the double Ala at the signal and mature peptide linkage site. Then separate signal peptide from mature peptide. Finally, secreting out mature peptide into the culture medium." href="">Signal peptide (<I>S.lividans chitinase C</I>)</a></td>
 
           <td>93 bp</td>   
 
           <td>93 bp</td>   
 
         </tr>
 
         </tr>
Line 97: Line 97:
 
           <td><a  href="http://parts.igem.org/Part:BBa_K1727005">BBa_K1727005</a></td>
 
           <td><a  href="http://parts.igem.org/Part:BBa_K1727005">BBa_K1727005</a></td>
 
           <td>Coding</td>
 
           <td>Coding</td>
−
           <td><a title="Signiferin is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Crinia signifera to against microbes. Signiferin use it's chargeability to interact with bacteria cell membrane. Than use hydrophobic region interfere the membrane structure. This leads to cell lysis and bypasses bacterial antibiotic drug-resistance mechanisms. Signiferin has demonstrated effectiveness in killing Methicillin-Resistant Staphylococcus aureus (MRSA), and has been proven by the TU-Delft 2013 iGEM team." href="">Signiferin (Crinia signifera)</a></td>
+
           <td><a title="Signiferin is a kind of antimicrobial peptide (AMPs) and it is a stable peptides that have extensive abilities to kill or inhibit the growth of bacteria. They play a role in defense mechanism for Crinia signifera to against microbes. Signiferin use it's chargeability to interact with bacteria cell membrane. Than use hydrophobic region interfere the membrane structure. This leads to cell lysis and bypasses bacterial antibiotic drug-resistance mechanisms. Signiferin has demonstrated effectiveness in killing Methicillin-Resistant Staphylococcus aureus (MRSA), and has been proven by the TU-Delft 2013 iGEM team." href="">Signiferin (<I>Crinia signifera</I>)</a></td>
 
           <td>275 bp</td>
 
           <td>275 bp</td>
 
         </tr>
 
         </tr>

Revision as of 15:52, 12 September 2015

This year TCU_Taiwan iGEM team had built new standard biological parts for iGEM community. Click on the name of the parts for detailed information.

Composite parts table

Favorite Name Type Description Legth
  BBa_K1727007 Composite Promoter+Lac operator+RBS+signal peptide
+Epinecidin-1+Terminater		 216 bp
  BBa_K1727008 Composite Promoter+Lac operator+RBS+Signal peptide
+Signiferin+Terminater		 179 bp


We used T5 promoter, Lac operator, RBS, terminator on pQE 60 vectors. So we can use IPTG induction to get our product.


We used T5 promoter, Lac operator, RBS, terminator on pQE 60 vectors. So we can use IPTG induction to get our product.



             
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