Difference between revisions of "Team:UC Davis"

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<a href="https://2015.igem.org/Team:UC_Davis"style="text-decoration:none;color:#FFFFFF">HOME </a> </td>  
 
<a href="https://2015.igem.org/Team:UC_Davis"style="text-decoration:none;color:#FFFFFF">HOME </a> </td>  
  
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<a href="http://https://2015.igem.org/Team:UCDavis/Project" style="text-decoration:none;color:#FFFFFF">PROJECT</a> </td>
 
<a href="http://https://2015.igem.org/Team:UCDavis/Project" style="text-decoration:none;color:#FFFFFF">PROJECT</a> </td>
  
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<a href="https://2015.igem.org/Team:UCDavis/Parts" style="text-decoration:none;color:#FFFFFF">PARTS</a></td>  
 
<a href="https://2015.igem.org/Team:UCDavis/Parts" style="text-decoration:none;color:#FFFFFF">PARTS</a></td>  
  
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<a href="https://2015.igem.org/Team:UC_Davis/Attributions" style="text-decoration:none;color:#FFFFFF">TEAM </a> </td>
 
<a href="https://2015.igem.org/Team:UC_Davis/Attributions" style="text-decoration:none;color:#FFFFFF">TEAM </a> </td>
  
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<a href="https://2014.igem.org/WikitemplateB_notebook" style="text-decoration:none;color:#FFFFFF">NOTEBOOK </a> </td>
 
<a href="https://2014.igem.org/WikitemplateB_notebook" style="text-decoration:none;color:#FFFFFF">NOTEBOOK </a> </td>
  
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<a href="https://2014.igem.org/WikitemplateB_safety" style="text-decoration:none;color:#FFFFFF">SAFETY </a></td>
 
<a href="https://2014.igem.org/WikitemplateB_safety" style="text-decoration:none;color:#FFFFFF">SAFETY </a></td>
 
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<h3>Read about our projects!</h3>
 
<h3>Read about our projects!</h3>
  
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One E.coli Has One Function
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We are developing a biosensor for triclosan, an antimicrobial agent whose use has environmental and human health implications. Triclosan inhibits enoyl acp reductase (Fabi). By measuring the rate at which triclosan inhibits Fabi, we can make a standard curve of percent inhibition vs triclosan, and use this standard curve to determine how much triclosan is present in a wastewater sample. We are investigating the activity of the Fabi enzyme from 8 different organisms on a panel of non-native substrates to determine the combination of Fabi and substrate that will yield the best results at the lowest cost. We can then engineer the enzyme to have greater specificity on the non-native substrate through computational design and kunkel mutagenesis. By coupling our biosensor with civic engagement measures, our goal is to raise awareness and accountability around environmental concerns and to empower citizens to participate in decisions being made about chemical use.
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What is our project at 2014?
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Cadmium is one of harmful materialｓ for us. 50 years ago, itai-iati disease was going around in center of Japan Gihu. The cause was industrial wastewater. Cadmium contained the water. Our project is to find a way to clean contaminated with Cadmium. we think how to clean the water. We use E.coli.
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Concretely, we use two kinds of E.coli. One catches Cadmium.　the Other makes to all E.coli to use chemoattractant. Catches E.coli displays metallothionein (metallothionein is a protein that combines a heavy metal. Cadomium is one kind of heavy metal). Other is releasing Asp (Asp is one kind of chemoattractant. All E.coli is close to the Asp E.coli).
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To use these E.coli. Finally cadomiun is catched(wastewater is be clean).
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What experiment we did?
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・How to check E.coli's chemotaxis?
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We make use of other igem team’s　assay.
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Using soft agar plate.we let E.coli siwm.
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・We made a plasmid
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Our project need to two kind of plasmid.
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we made one plasmid.And we have to make one more plasmid.
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<a href="#firstblock" style="text-decoration:none;color:#FFFFFF"> Top </a> </td></tr>
 
<a href="#firstblock" style="text-decoration:none;color:#FFFFFF"> Top </a> </td></tr>
 
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Revision as of 03:32, 13 September 2015

Read about our projects!

We are developing a biosensor for triclosan, an antimicrobial agent whose use has environmental and human health implications. Triclosan inhibits enoyl acp reductase (Fabi). By measuring the rate at which triclosan inhibits Fabi, we can make a standard curve of percent inhibition vs triclosan, and use this standard curve to determine how much triclosan is present in a wastewater sample. We are investigating the activity of the Fabi enzyme from 8 different organisms on a panel of non-native substrates to determine the combination of Fabi and substrate that will yield the best results at the lowest cost. We can then engineer the enzyme to have greater specificity on the non-native substrate through computational design and kunkel mutagenesis. By coupling our biosensor with civic engagement measures, our goal is to raise awareness and accountability around environmental concerns and to empower citizens to participate in decisions being made about chemical use.
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