Thursday 9th July

Lab Work

Transformation

by Coralie

Ligation product:

• BBa_J23101 + BBa_I13504
• BBa_J23106 + BBa_I13504
• BBa_J23117 + BBa_I13504

On LB + Chloramphenicol 20ug/mL. Incubation ON, 37°C

by Johan and Seong Ko

• BBa_R0051

On LB + Ampicillin 100ug/mL. Incubation ON, 37°C

Plasmid Rehydratation

by Johan and Audrey

• BBa_S03518
• BBa_B0030
• BBa_B0015
• BBa_K1399005

Digestion

by Pauline and Audrey

Plasmid with promotor: BBa_J23101

• 10µL of our plasmid with promotor
• 1µL SpeI
• 1µL PstI
• 2µL buffer 10x FD

Plasmid with gene: BBa_COO40 and BBa_K115017

• 10µL of our plasmid with gene
• 1µL XbaI
• 1µL PstI
• 2µL buffer 10x FD

After this step, we separate the sequence we need from the sequence we don't with electrophoresis

Electrophoresis

by Pauline and Audrey

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5µL of BET Migration 0,06A 80V

Quantification

by Pauline and Audrey

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5µL of BET Migration 0,06A 80V We quantify with the electrophoresis gel:

• BBa_C0040: 10ng/µL
• BBa_J23101: 20ng/µL
• BBa_K115017: we don't see enough of fluorescent to quantify the biobrick. We decide to amplify this one by PCR.

Members present:

• Instructors and advisors: Alice.
• Students: Johan, Seong Koo, Audrey, Coralie, Pauline

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