Difference between revisions of "Team:Tianjin/GFP"

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Saturday.6.6.2015

PCR on PSB1C3

gene of interst：GFP

primer: Xba1-GFP、GFP-linker

Xba1-GFP: 5’-GCTCTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTC

GFP-linker: 5’-AGAACCACCACCACCAGAACCACCACCACCTTTGTATAGTTCATC

PCR system：（50μl）

Buffer	5μl
dNTPS	5μl
Xba1-GFP primer	2μl
GFP-linker primer	2μl
GFP PSB1C3	1μl
Pfu enzyme	0.5μl
ddH2O	34.5μl

cycle

95℃	5min
95℃	30s
1：50℃ 2：55℃ 3：58℃ 4：60℃	30s
72℃	2min
72℃	10min
4℃	Forever

Electrophoresis of the PCR products was identified, and finally PCR product was purified by gel extraction kit to prepare for digestion.

qualitative agarose gel electrophpresis：GFP on 55, 58, 60 centigrade worked well.

(The maximum sequence of the maker is 2000bp, and following is 1500, 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 750bp approximately.)

Electrophoresis of the PCR products was identified, and finally PCR product was purified by gel extraction kit to prepare for digestion.

Fragment concentration: 51.7 ng/μL

Date: Monday,7.13.2015

PCR on pET28a(+)

gene of interst：sJanus

primer：linker-sJanus、sJanus-Pst1

linker-sJanus:5’-GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTAGCAACGGCAACGGCAA

sJanus-Pst1:5’- AACTGCAGCGGCCGCTACTAGTATCACCGACGGCGGTCTG

PCR system：（50μl）

Buffer	5μl
dNTPS	5μl
linker- sJanus primer	2μl
sJanus -Pst1 primer	2μl
sJanus pET28a(+)	1μl
Pfu enzyme	0.5μl
ddH2O	34.5μl

cycle

95℃	5min
95℃	30s
1：50℃ 2：55℃ 3：58℃ 4：60℃	30s
72℃	2min
72℃	10min
4℃	Forever

qualitative agarose gel electrophpresis：sJanus on all centigrades worked

(The maximum sequence of the maker is 2000bp, and following is 1500, 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 250bp approximately.)

Electrophoresis of the PCR products was identified, and finally PCR product was purified by gel extraction kit to prepare for digestion.

Fragment concentration:70.2ng/μL

Date: Monday,7.13.2015

PCR on pET28a(+)

gene of interst：sJanus-m

primer：linker- sJanus、sJanus-Pst1

linker- sJanus:5’-GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTAGCAACGGCAACGGCAA

SJANUS-Pst1:5’- AACTGCAGCGGCCGCTACTAGTATCACCGACGGCGGTCTG

PCR system：（50μl）

Buffer	5μl
dNTPS	5μl
linker- sJanus primer	2μl
sJanus -Pst1 primer	2μl
sJanus-m pET28a(+)	1μl
Pfu enzyme	0.5μl
ddH2O	34.5μl

cycle

95℃	5min
95℃	30s
1：50℃ 2：55℃ 3：58℃ 4：60℃	30s
72℃	2min
72℃	10min
4℃	Forever

qualitative agarose gel electrophpresis：sJanus-m on all centigrades worked

（The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 250bp approximately.）

Electrophoresis of the PCR products was identified, and finally PCR product was purified by gel extraction kit to prepare for digestion.

Fragment concentration:70.6ng/μL

Date: Monday,7.13.2015

PCR on pET28a(+)

gene of interst：inJanus

primer：linker- inJanus、inJanus-Pst1

linker- inJanus: 5’- GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTCAACAGTGCACCACT

INJANUS-Pst15’- GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTCAACAGTGCACCACT

PCR system：（50μl）

Buffer	5μl
dNTPS	5μl
BFP_F primer	2μl
BFP_R primer	2μl
BFP pSB1C3	1μl
Pfu enzyme	0.5μl
ddH2O	34.5μl

cycle

95℃	5min
95℃	30s
1:50.1℃ 2:53.4℃ 3:56.4℃ 4:58.5℃	30s
72℃	2min
72℃	10min
4℃	Forever

qualitative agarose gel electrophpresis：inJanus didn’t work well. The electrophoretic bands towing phenomenon is obvious.

(The maximum sequence of the maker is 2000bp, and following is 1500, 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 250bp approximately.)

analyse： We suspect is caused by low temperature. So we raise the temperature to conduct the PCR later.

Date: Tuesday,7.14.2015

PCR on pET28a(+)

gene of interst：inJanus

primer：linker- inJanus、inJanus-Pst1

linker- inJanus:

5’- GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTCAACAGTGCACCACT

inJanus-Pst15’- GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTCAACAGTGCACCACT

PCR system：（50μl）

Buffer	5μl
dNTPS	5μl
linker- inJanus primer	2μl
inJanus -Pst1 primer	2μl
inJanus pET28a(+)	1μl
Pfu enzyme	0.5μl
ddH2O	34.5μl

cycle

95℃	5min
95℃	30s
1:59.4℃ 2:65.4℃ 3:67.0℃	30s
72℃	2min
72℃	10min
4℃	Forever

qualitative agarose gel electrophpresis：inJanus-m worked

（The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 250bp approximately.）

Electrophoresis of the PCR products was identified, and finally PCR product was purified by gel extraction kit to prepare for digestion.

Fragment concentration:43.5ng/μL

Date: Tuesday,7.14.2015

PCR on pET28a(+)

gene of interst：inJanus-m

primer：linker-inJanus-m、inJanus-Pst1

linker-inJanus-m:5’-GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTCAACAGTCTACCACTGGC

inJanus

-Pst15’- GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTCAACAGTGCACCACT

PCR system：（50μl）

Buffer	5μl
dNTPS	5μl
linker-inJanus-m primer	2μl
inJanus -Pst1 primer	2μl
inJanus-m pET28a(+)	1μl
Pfu enzyme	0.5μl
ddH2O	34.5μl

cycle

95℃	5min
95℃	30s
1:59.4℃ 2:65.4℃ 3:67.0℃	30s
72℃	2min
72℃	10min
4℃	Forever

qualitative agarose gel electrophpresis：inJanus-m on these three centigrades worked

（The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 250bp approximately.）

Electrophoresis of the PCR products was identified, and finally PCR product was purified by gel extraction kit to prepare for digestion.

Fragment concentration:55.0ng/μL

Date: Wednesday,7.15.2015

SOE PCR

SOE PCR stands for Splicing by Overlapp Extension PCR. It is a standard overlapp extension procedure, enabling the assembly of genes wihtout performing any cloning, digesting or ligation inbetween. All you need to do ist running PCRs with specific primers. If gene A is the upstream part and gene B has to be assembled downstream of gene A, primer lo of gene A should have an overlapp of around 20 nucleotides complementary to the first 20 nucleotides of gene B. Primer up of gene B should haven a complementary overlapp of 20 nucleotides to the end of gene A.

gene of interst：GFP- sJanus

primer：Xba1-GFP、sJanus-Pst1

temperature: 53, 55, 56, and 58 centigrade.

PCR system（100μl）

Buffer	10μl
dNTPS	10μl
GFP fragment	5μl
sJanus fragment	5μl
Pfu enzyme	1μl
ddH2O	69μl

Add two kinds of primer (1μl) after 5 cycle.

cycle

95℃	5min
95℃	30s
1：50℃ 2：56℃ 3：65℃	30s
72℃	2min
72℃	10min
95℃	5min
95℃	30s
1：50℃ 2：56℃ 3：65℃	30s
72℃	2min
72℃	10min
4℃	Forever

qualitative agarose gel electrophpresis：GFP－sJanus for SOE　PCR with PSB1C3 worked

The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 1000bp approximately.

Electrophoresis of the PCR products was identified, and finally PCR product was purified by gel extraction kit to prepare for digestion.

Fragment concentration:32.2ng/μL

Date: Wednesday,7.15.2015

SOE PCR

gene of interst：GFP-sJanus-m

primer：Xba1-GFP、sJanus -Pst1

temperature： 53, 55, 56, and 58 centigrade.

PCR system（100μl）

Buffer	10μl
dNTPS	10μl
GFP fragment	5μl
sJanus-m fragment	5μl
Pfu enzyme	1μl
ddH2O	69μl

Add two kinds of primer (1μl) after 5 cycle.

cycle

95℃	5min
95℃	30s
1：50℃ 2：56℃ 3：65℃	30s
72℃	2min
72℃	10min
95℃	5min
95℃	30s
1：50℃ 2：56℃ 3：65℃	30s
72℃	2min
72℃	10min
4℃	Forever

qualitative agarose gel electrophpresis：GFP－sJanus-m for SOE　PCR with PSB1C3 worked

The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 1000bp approximately.

Electrophoresis of the PCR products was identified, and finally PCR product was purified by gel extraction kit to prepare for digestion.

Fragment concentration: 30.5ng/μL

Date: Wednesday,7.15.2015

SOE PCR

gene of interst：GFP- sJanus

primer：Xba1-GFP、inJanus-Pst1

temperature： 55, 56.3 ,57.8 and 58.9 centigrade.

PCR system（100μl）

Buffer	10μl
dNTPS	10μl
GFP fragment	5μl
inJanus fragment	5μl
Pfu enzyme	1μl
ddH2O	69μl

Add two kinds of primer (1μl) after 5 cycle.

cycle

95℃	5min
95℃	30s
1：55℃ 2：56.3℃ 3：57.8℃ 4：58.9℃	30s
72℃	2min
72℃	10min
95℃	5min
95℃	30s
1：50℃ 2：56℃ 3：65℃	30s
72℃	2min
72℃	10min
4℃	Forever

qualitative agarose gel electrophpresis：GFP－inJanus for SOE PCR with PSB1C3 worked

（The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 1000bp approximately.）

Electrophoresis of the PCR products was identified, and finally PCR product was purified by gel extraction kit to prepare for digestion.

Fragment concentration:31.1ng/μL

Date：Wednesday,7.15.2015

SOE PCR

gene of interst：GFP-inJanus

primer：Xba1-GFP、inJanus-Pst1

temperature： 55, 56.3 ,57.8 and 58.9 centigrade.

PCR system（100μl）

Buffer	10μl
dNTPS	10μl
GFP fragment	5μl
inJanus-m fragment	5μl
Pfu enzyme	1μl
ddH2O	69μl

Add two kinds of primer (1μl) after 5 cycle.

cycle

95℃	5min
95℃	30s
1：55℃ 2：56.3℃ 3：57.8℃ 4：58.9℃	30s
72℃	2min
72℃	10min
95℃	5min
95℃	30s
1：50℃ 2：56℃ 3：65℃	30s
72℃	2min
72℃	10min
4℃	Forever

qualitative agarose gel electrophpresis：GFP－inJanus-m for SOE　PCR with PSB1C3 worked

（The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 1000bp approximately.）

Electrophoresis of the PCR products was identified, and finally PCR product was purified by gel extraction kit to prepare for digestion.

Fragment concentration:50.3ng/μL

Date: Thursday,7.16.2015

restriction enzyme digestion

After qualitative agarose gel electrophpresis, all the target sequence are 1000bp approximately.

Fragment concentration: sJanus is 6.9ng/μL, sJanus-m is 15.3ng/μL, inJanus is 4.6ng/μL, inJanus-m is 7.0ng/μL.

Date: Friday,7.17.2015

We used T4 DNA ligase to connect them at the temperature of 22 centigrade about an hour.

Transformed the expression vectors we had already connected into the Escherichia coli DH5α, then incubated the bacteria for 12h (37℃).

Date:Saturday,7.18.2015

Enzymes cut each, but they did not work.

（The maximum of marker is 8000bp, and following is 8000, 5000, 3000,1500,1000and 500bp from up and down, our target sequence is about 1000bp approximately. But the bands in this figure are all about 5000bp, so we suspect that the fragments are not connected to plasmid.）

Analyse：The fragments are not connected to plasmid.

Date: Sunday,7.19.2015

We chose another DH5α and trans

formed again.

Date: Monday,7.20.2015

Enzyme cut,

Date: Tuesday,7.21.2015

We design another kind of primer and reverse which contain EcoRI and XhoI site. We have got the standard GFP-sJanus and GFP-sJanus-m fragments, and we got the target fragments used to construct the expression vector by PCR.

gene of interst：EcoR1-GFP-sJanus -Xho1、EcoR1-GFP-sJanus-m-Xho1

primer：EcoR1-GFP、sJanus-Xho1

EcoR1-GFP: 5’-CGGAATTCATGCGTAAAGGAGAAGAACTT

sJanus-Xho1: 5’-CCGCTCGAGTCAAGCACCGACGGCC

PCR system：（50μl）

Buffer	5μl
dNTPS	5μl
EcoR1-GFP primer	2μl
sJanus-Xho1 primer	2μl
Xba1-GFP-sJanus -Pst1/ Xba1-GFP-sJanus-m-Pst1	1μl
Pfu enzyme	0.5μl
ddH2O	34.5μl

cycle

95℃	5min
95℃	30s
1:55.4℃ 2:56.6℃ 3:57.8℃ 4:60.3℃	30s
72℃	2min
72℃	10min
4℃	Forever

qualitative agarose gel electrophpresis：fragments on these four centigrades all worked well.

（The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 1000bp approximately.）

Electrophoresis of the PCR products was identified, and finally PCR product was purified by gel extraction kit to prepare for digestion.

Fragment concentration:EcoR1-GFP-sJanus -Xho1:55.0ng/μL、EcoR1-GFP-sJanus-m-Xho1:60ng/μL

Date: Saturday,8.1.2015

We used enzymes of EcoRI site and XhoI site to digest the PCR product and plasmid. We used T4 DNA ligase to connect them at the temperature of 22 centigrade for about one hour. Then we transformed the expression vectors we had already connected into the Escherichia coli DH5α, then incubated the bacteria for 12h (37℃)

It has been confirmed that these sequences we get are right when detecting them in company.

Date:Monday,8.10.2015

Transformed the expression vectors(concentration:140.3ng/μL、121.4ng/μL) we had already built before (pET28a(+)) into the Escherichia coli BL21, then incubated the bacteria for 12h (37℃).

2

3

4

5

@@ Line 273: / Line 273: @@
 	    <div id="content-in">
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px;">1.Get the gene of BFP</span></strong></span>
+     <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px;">Saturday.6.6.2015</span></strong></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1.1 In order to get the fusion protein, we use overlap PCR to construct the fragment(BFP- sJanus &amp; BFP-sJanus-m).We get the gene of BFP by the standard plasmid from iGEM. Then we use PCR to amplify it. The primer we design is as follow:</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">PCR on PSB1C3</span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">F:5’- GAATTCGCGGCCGCTTCTAGATGAGCGAACTGATCAAAGAG-3’</span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">gene of interst</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">GFP</span></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">(EcoRI and XbaI site are contained). </span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">primer: Xba1-GFP</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">、</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">GFP-linker</span></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">R:5’- GTGGTGGTGGTCTTGGTGGTGGTGGATTCAGTTTATGACCCAGCTT-3’</span>
+     <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Xba1-GFP: 5’-GCTCTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTC</span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">(linker-GGGGSGGGGS is contained)</span>
+     <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">GFP-linker: 5’-AGAACCACCACCACCAGAACCACCACCACCTTTGTATAGTTCATC</span>
+</p>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR system</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：（</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">50μl</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
+</p>
+<table align="center" cellpadding="0" cellspacing="0" width="269">
+    <tbody>
+        <tr class="firstRow">
+            <td style="border: 1px solid black; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Buffer</span>
+                </p>
+            </td>
+            <td style="border-width: 1px; border-style: solid; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: 1px; border-style: solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">dNTPS</span>
+                </p>
+            </td>
+            <td style="border-width: 1px; border-style: solid; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: 1px; border-style: solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Xba1-GFP primer</span>
+                </p>
+            </td>
+            <td style="border-width: 1px; border-style: solid; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: 1px; border-style: solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">GFP-linker primer</span>
+                </p>
+            </td>
+            <td style="border-width: 1px; border-style: solid; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: 1px; border-style: solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">GFP PSB1C3</span>
+                </p>
+            </td>
+            <td style="border-width: 1px; border-style: solid; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: 1px; border-style: solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Pfu enzyme</span>
+                </p>
+            </td>
+            <td style="border-width: 1px; border-style: solid; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">0.5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: 1px; border-style: solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">ddH2O</span>
+                </p>
+            </td>
+            <td style="border-width: 1px; border-style: solid; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">34.5μl</span>
+                </p>
+            </td>
+        </tr>
+    </tbody>
+</table>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">cycle</span>
+</p>
+<table align="center" cellpadding="0" cellspacing="0" width="269">
+    <tbody>
+        <tr class="firstRow">
+            <td style="border: 1px solid black; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">95</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">95</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">1</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">50</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">2</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">55</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">3</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">58</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">4</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">60</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">72</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">72</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">4</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Forever</span>
+                </p>
+            </td>
+        </tr>
+    </tbody>
+</table>
+<p style="line-height: 1.5em;">
+    <span style="font-size: 19px; color: windowtext; border: 1px none windowtext; padding: 0px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">Electrophoresis</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">of the</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">PCR</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">products</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">was</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">identified</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">, and&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">finally</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">PCR</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">product</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">was purified by</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">gel</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">extraction</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">kit</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">to</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">prepare for</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">digestion</span>.</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">qualitative agarose gel electrophpresis</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">GFP on 55, 58, 60 centigrade worked well.</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+</p>
+<p style="text-align: center; line-height: 1.5em;">
+    <img alt="demo.jpg" src="/server/ueditor/upload/image/demo.jpg" title=""/>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"></span><br/>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">(The maximum sequence of the maker is 2000bp, and following is 1500, 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 750bp approximately.)</span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;">&nbsp;<span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">Electrophoresis</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">of the</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">PCR</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">products</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">was</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">identified</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">, and&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">finally</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">PCR</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">product</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">was purified by</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">gel</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">extraction</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">kit</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">to</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">prepare for</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">digestion</span>.</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Fragment concentration: 51.7 ng/μL</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date: </span></strong><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Monday,7.13.2015</span></strong></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR on pET28a(+)</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">gene of interst</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">primer</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">linker-sJanus</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">、</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus-Pst1</span></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">We get the target sequence by PCR at the temperature of 55 ,60 and 65 centigrade.</span>
+     <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">linker-sJanus:5’-GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTAGCAACGGCAACGGCAA</span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1.2 detail of the experiment</span>
+     <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus-Pst1:5’- AACTGCAGCGGCCGCTACTAGTATCACCGACGGCGGTCTG</span>
 </p>
 <p style="text-align: center; line-height: 1.5em;">
-     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">PCR system</span><span style="font-size: 19px; font-family: 宋体;">：（</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">50μl</span><span style="font-size: 19px; font-family: 宋体;">）</span></span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR system</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：（</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">50μl</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
 </p>
-<table align="center" cellpadding="0" cellspacing="0">
+<table align="center" cellpadding="0" cellspacing="0" width="269">
      <tbody>
          <tr class="firstRow">
-             <td style="border: 1px solid windowtext; padding: 0px 7px;" valign="top" width="136">
+             <td style="border: 1px solid black; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Buffer</span>
                  </p>
              </td>
-             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: windowtext windowtext windowtext -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
@@ Line 314: / Line 535: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">dNTPS</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
@@ Line 326: / Line 547: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">BFP_F primer</span>
+                     <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">linker- sJanus primer</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
@@ Line 338: / Line 559: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">BFP_R primer</span>
+                     <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus -Pst1 primer</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
@@ Line 350: / Line 571: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">BFP PSB1C3</span>
+                     <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus pET28a(+)</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1μl</span>
@@ Line 362: / Line 583: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Pfu enzyme</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">0.5μl</span>
@@ Line 374: / Line 595: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">ddH2O</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">34.5μl</span>
@@ Line 387: / Line 608: @@
      </tbody>
 </table>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">cycle</span>
+</p>
+<table align="center" cellpadding="0" cellspacing="0" width="269">
+    <tbody>
+        <tr class="firstRow">
+            <td style="border: 1px solid black; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">1</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">50</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">2</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">55</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">3</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">58</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">4</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">60</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Forever</span>
+                </p>
+            </td>
+        </tr>
+    </tbody>
+</table>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;">&nbsp;<span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">qualitative agarose gel electrophpresis</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus on all centigrades worked</span></span>
+</p>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"><img alt="demo.jpg" src="/server/ueditor/upload/image/demo.jpg" title=""/></span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">(The maximum sequence of the maker is 2000bp, and following is 1500, 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 250bp approximately.)</span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">Electrophoresis</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">of the</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">PCR</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">products</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">was</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">identified</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">, and&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">finally</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">PCR</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">product</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">was purified by</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">gel</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">extraction</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">kit</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">to</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">prepare for</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">digestion</span>.</span></span>
+</p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Fragment concentration:70.2ng/μL</span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+     <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date: </span></strong><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Monday,7.13.2015</span></strong></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR on pET28a(+)</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">gene of interst</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus-m</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">primer</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">linker- sJanus</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">、</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus-Pst1</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">linker- sJanus:5’-GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTAGCAACGGCAACGGCAA</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">SJANUS-Pst1:5’- AACTGCAGCGGCCGCTACTAGTATCACCGACGGCGGTCTG</span>
+</p>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR system</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：（</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">50μl</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
+</p>
+<table align="center" cellpadding="0" cellspacing="0" width="269">
+    <tbody>
+        <tr class="firstRow">
+            <td style="border: 1px solid black; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Buffer</span>
+                </p>
+            </td>
+            <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">dNTPS</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">linker- sJanus primer</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus -Pst1 primer</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">sJanus-m pET28a(+)</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Pfu enzyme</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">0.5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">ddH2O</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">34.5μl</span>
+                </p>
+            </td>
+        </tr>
+    </tbody>
+</table>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">cycle</span>
 </p>
-<table align="center" cellpadding="0" cellspacing="0">
+<table align="center" cellpadding="0" cellspacing="0" width="269">
      <tbody>
          <tr class="firstRow">
-             <td style="border: 1px solid windowtext; padding: 0px 7px;" valign="top" width="136">
+             <td style="border: 1px solid black; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: windowtext windowtext windowtext -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
@@ Line 408: / Line 838: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
@@ Line 420: / Line 850: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">1</span><span style="font-size: 19px; font-family: 宋体;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">55</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">1</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">50</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">2</span><span style="font-size: 19px; font-family: 宋体;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">60</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">2</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">55</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">3</span><span style="font-size: 19px; font-family: 宋体;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">65</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">3</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">58</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
-            </td>
-            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">4</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">60</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
@@ Line 441: / Line 871: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
@@ Line 453: / Line 883: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
@@ Line 465: / Line 895: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">4</span><span style="font-size: 19px; font-family: &quot;微软雅黑&quot;,sans-serif;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Forever</span>
@@ Line 478: / Line 908: @@
      </tbody>
 </table>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">qualitative agarose gel electrophpresis</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus-m on all centigrades worked</span></span>
+</p>
 <p style="text-align: center; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"><img alt="demo.jpg" src="/server/ueditor/upload/image/demo.jpg" title=""/></span>
+</p>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">（</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 250bp approximately.</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">Electrophoresis</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">of the</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">PCR</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">products</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">was</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">identified</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">, and&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">finally</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">PCR</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">product</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">was purified by</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">gel</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">extraction</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">kit</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">to</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">prepare for</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">digestion</span>.</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Fragment concentration:70.6ng/μL</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date: </span></strong><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Monday,7.13.2015</span></strong></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR on pET28a(+)</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">gene of interst</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">inJanus</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">primer</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">linker- inJanus</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">、</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">inJanus-Pst1</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">linker- inJanus: 5’- GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTCAACAGTGCACCACT</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">INJANUS-Pst15’- GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTCAACAGTGCACCACT</span>
+</p>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR system</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：（</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">50μl</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
+</p>
+<table align="center" cellpadding="0" cellspacing="0" width="269">
+    <tbody>
+        <tr class="firstRow">
+            <td style="border: 1px solid black; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Buffer</span>
+                </p>
+            </td>
+            <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">dNTPS</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">BFP_F primer</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">BFP_R primer</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">BFP pSB1C3</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Pfu enzyme</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">0.5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">ddH2O</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">34.5μl</span>
+                </p>
+            </td>
+        </tr>
+    </tbody>
+</table>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">cycle</span>
+</p>
+<table align="center" cellpadding="0" cellspacing="0" width="269">
+    <tbody>
+        <tr class="firstRow">
+            <td style="border: 1px solid black; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">1:50.1</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">2:53.4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">3:56.4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">4:58.5</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Forever</span>
+                </p>
+            </td>
+        </tr>
+    </tbody>
+</table>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">2.Get the gene of BFP- sJanus &amp; BFP-sJanus-m</span></strong></span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">qualitative agarose gel electrophpresis</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">inJanus didn’t work well. The electrophoretic bands towing phenomenon is obvious.</span></span>
+</p>
+<p style="text-align: center; line-height: 1.5em;">
+    <img alt="demo.jpg" src="/server/ueditor/upload/image/demo.jpg" title=""/>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2.1We get the gene of BFP- sJanus &amp; BFP-sJanus-m by the plasmid pET28a(+) extracting from DH5α. And then we amplify it by PCR. The primer we design is as follow:</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"><br/></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">F:5’-ACCACCAAGACCACCACCACCAAGAAGCAACGGCAACGGCAAT-3’</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">(The maximum sequence of the maker is 2000bp, and following is 1500, 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 250bp approximately.)</span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">(linker-GGGGSGGGGS is contained)</span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">analyse</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;"> We suspect is caused by low temperature. So we raise the temperature to conduct the PCR later.</span></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">R:5’-CTGCAGGCGGCCGCTACTAGTATCAAGCACCGACGGCGG-3’</span>
+     <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date: </span></strong><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Tuesday,7.14.2015</span></strong></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">( SpeI, NotI, and PstI site are contained)</span>
+     <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR on pET28a(+)</span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">We get the target sequence by PCR at the temperature of 55, 60 and 65 centigrade.</span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">gene of interst</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">inJanus</span></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2.2 Details of experiment</span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">primer</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">linker- inJanus</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">、</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">inJanus-Pst1</span></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+     <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">linker- inJanus:</span>
 </p>
-<p style="text-align: center; line-height: 1.5em;">
+<p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5’- GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTCAACAGTGCACCACT</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">inJanus-Pst15’- GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTCAACAGTGCACCACT</span>
 </p>
 <p style="text-align: center; line-height: 1.5em;">
-     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">PCR system</span><span style="font-size: 19px; font-family: 宋体;">：（</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">50μl</span><span style="font-size: 19px; font-family: 宋体;">）</span></span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR system</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：（</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">50μl</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
 </p>
-<table align="center" cellpadding="0" cellspacing="0">
+<table align="center" cellpadding="0" cellspacing="0" width="269">
      <tbody>
          <tr class="firstRow">
-             <td style="border: 1px solid windowtext; padding: 0px 7px;" valign="top" width="136">
+             <td style="border: 1px solid black; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Buffer</span>
                  </p>
              </td>
-             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: windowtext windowtext windowtext -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
@@ Line 529: / Line 1,177: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">dNTPS</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
@@ Line 541: / Line 1,189: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">P_F</span>
+                    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">linker- inJanus</span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">primer</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
@@ Line 553: / Line 1,204: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">P_R</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">inJanus</span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">-Pst1 primer</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
@@ Line 565: / Line 1,219: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">sJanus PET28a</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">inJanus</span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">pET28a(+)</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1μl</span>
                  </p>
              </td>
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Pfu enzyme</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">0.5μl</span>
@@ Line 589: / Line 1,246: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">ddH2O</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">33.5μl</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">34.5μl</span>
                  </p>
              </td>
@@ Line 602: / Line 1,259: @@
      </tbody>
 </table>
-<p style="text-align: left; line-height: 1.5em;">
+<p style="text-align: center; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">cycle</span>
 </p>
-<table align="center" cellpadding="0" cellspacing="0">
+<table align="center" cellpadding="0" cellspacing="0" width="269">
      <tbody>
          <tr class="firstRow">
-             <td style="border: 1px solid windowtext; padding: 0px 7px;" valign="top" width="136">
+             <td style="border: 1px solid black; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: windowtext windowtext windowtext -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
@@ Line 620: / Line 1,277: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
@@ Line 632: / Line 1,289: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">1</span><span style="font-size: 19px; font-family: 宋体;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">55</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">1:59.4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">2</span><span style="font-size: 19px; font-family: 宋体;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">60</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">2:65.4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">3</span><span style="font-size: 19px; font-family: 宋体;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">65</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">3:67.0</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
                  </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Forever</span>
+                </p>
+            </td>
+        </tr>
+    </tbody>
+</table>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">qualitative agarose gel electrophpresis</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">inJanus-m worked</span></span>
+</p>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"><img alt="demo.jpg" src="/server/ueditor/upload/image/demo.jpg" title=""/></span>
+</p>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">（</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 250bp approximately.</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">Electrophoresis</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">of the</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">PCR</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">products</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">was</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">identified</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">, and&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">finally</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">PCR</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">product</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">was purified by</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">gel</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">extraction</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">kit</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">to</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">prepare for</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">digestion</span>.</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Fragment concentration:43.5ng/μL</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date</span></strong><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">: Tuesday,7.14.2015</span></strong></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR on pET28a(+)</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">gene of interst</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">inJanus-m</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">primer</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">linker-inJanus-m</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">、</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">inJanus-Pst1</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">linker-inJanus-m:5’-GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTCAACAGTCTACCACTGGC</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">inJanus</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">-Pst15’- GGTGGTGGTGGTTCTGGTGGTGGTGGTTCTCAACAGTGCACCACT</span>
+</p>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR system</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：（</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">50μl</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
+</p>
+<table align="center" cellpadding="0" cellspacing="0" width="269">
+    <tbody>
+        <tr class="firstRow">
+            <td style="border: 1px solid black; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Buffer</span>
+                </p>
+            </td>
+            <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">dNTPS</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">linker-inJanus-m primer</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">inJanus</span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">-Pst1 primer</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">inJanus-m pET28a(+)</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Pfu enzyme</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">0.5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">ddH2O</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">34.5μl</span>
+                </p>
+            </td>
+        </tr>
+    </tbody>
+</table>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">cycle</span>
+</p>
+<table align="center" cellpadding="0" cellspacing="0" width="269">
+    <tbody>
+        <tr class="firstRow">
+            <td style="border: 1px solid black; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
@@ Line 653: / Line 1,504: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">1:59.4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">2:65.4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">3:67.0</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
@@ Line 665: / Line 1,534: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
@@ Line 677: / Line 1,546: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Forever</span>
@@ Line 690: / Line 1,559: @@
      </tbody>
 </table>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">qualitative agarose gel electrophpresis</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">inJanus-m on these three centigrades worked</span></span>
+</p>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;<img alt="demo.jpg" src="/server/ueditor/upload/image/demo.jpg" title=""/></span>
+</p>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">（</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 250bp approximately.</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">Electrophoresis</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">of the</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">PCR</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">products</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">was</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">identified</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">, and&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">finally</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">PCR</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">product</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">was purified by</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">gel</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">extraction</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">kit</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">to</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">prepare for</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">digestion</span>.</span></span>
+</p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Fragment concentration:55.0ng/μL</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date: Wednesday,7.15.2015</span></strong></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">SOE PCR</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">SOE PCR stands for&nbsp;<em>Splicing by Overlapp Extension PCR</em>. It is a standard overlapp extension procedure, enabling the assembly of genes wihtout performing any cloning, digesting or ligation inbetween. All you need to do ist running PCRs with specific primers. If gene A is the upstream part and gene B has to be assembled downstream of gene A, primer lo of gene A should have an overlapp of around 20 nucleotides complementary to the first 20 nucleotides of gene B. Primer up of gene B should haven a complementary overlapp of 20 nucleotides to the end of gene A.</span>
+</p>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"><img alt="demo.jpg" src="/server/ueditor/upload/image/demo.jpg" title=""/></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">gene of interst</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">GFP- sJanus</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">primer</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Xba1-GFP</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">、</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus-Pst1</span></span>
 </p>
 <p style="line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2.3 Results</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">temperature: 53, 55, 56, and 58 centigrade.</span>
+</p>
+<p style="margin-left: 24px; text-align: center; text-indent: 0px; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">PCR system</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">（</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">100μl</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
+</p>
+<table align="center" cellpadding="0" cellspacing="0" width="269">
+    <tbody>
+        <tr class="firstRow">
+            <td style="border: 1px solid black; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Buffer</span>
+                </p>
+            </td>
+            <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">dNTPS</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">GFP&nbsp; fragment</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">sJanus fragment</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Pfu enzyme</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">ddH2O</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">69μl</span>
+                </p>
+            </td>
+        </tr>
+    </tbody>
+</table>
+<p style="margin-left: 24px; text-align: left; text-indent: 0px; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Add two kinds of primer (1μl) after 5 cycle.</span>
+</p>
+<p style="margin-left: 24px; text-align: center; text-indent: 0px; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">cycle</span>
+</p>
+<table align="center" cellpadding="0" cellspacing="0" width="269">
+    <tbody>
+        <tr class="firstRow">
+            <td style="border: 1px solid black; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">1</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">50</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">2</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">56</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">3</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">65</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">1</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">50</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">2</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">56</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">3</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">65</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Forever</span>
+                </p>
+            </td>
+        </tr>
+    </tbody>
+</table>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">qualitative agarose gel electrophpresis</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">GFP</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">－</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus for SOE</span>　<span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR with PSB1C3 worked</span></span>
 </p>
 <p style="text-align: center; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"><img alt="demo.jpg" src="https://static.igem.org/mediawiki/2015/d/d8/Tianjin_BFP_1.png" title=""/></span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"><img alt="demo.jpg" src="/server/ueditor/upload/image/demo.jpg" title=""/></span>
 </p>
 <p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
-     <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">Figure 1 </span></strong><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">The maximum of marker is 2000bp, and the centigrade of 60 is not suitable for the system because there is a track behind it. Our target is about 250bp approximately</span></span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 1000bp approximately.</span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">Electrophoresis</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">of the</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">PCR</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">products</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">was</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">identified</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">, and&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">finally</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">PCR</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">product</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">was purified by</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">gel</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">extraction</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">kit</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">to</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">prepare for</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">digestion</span>.</span></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">We get the target fragment in the end. All temperature were suitable for the PCR cycle.</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Fragment concentration:32.2ng/μL</span>
+</p>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">3.Overlap PCR</span></strong></span>
+     <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date: Wednesday,7.15.2015</span></strong></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">3.1 We use the technique of overlap PCR to get our target sequence. In this experiment, we add BFP and sJanus &amp; sJanus-m which we get in two steps and the BFP’s primer we design and sJanus &amp; sJanus-m’s reverse in the system of PCR. We get the target sequence at the temperature of 55, 60 and 65 centigrade.</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">SOE PCR</span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">3.2 Detail of the experiment</span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">gene of interst</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">GFP-sJanus-m</span></span>
 </p>
-<p style="margin-left: 24px; text-align: left; text-indent: 0px; line-height: 1.5em;">
+<p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">primer</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Xba1-GFP</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">、</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus -Pst1</span></span>
+</p>
+<p style="line-height: 1.5em;">
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">temperature</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;"> 53, 55, 56, and 58 centigrade.</span></span>
 </p>
 <p style="margin-left: 24px; text-align: center; text-indent: 0px; line-height: 1.5em;">
-     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">PCR system</span><span style="font-size: 19px; font-family: 宋体;">（</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">100μl</span><span style="font-size: 19px; font-family: 宋体;">）</span></span>
+     <span style="font-family: arial,helvetica,sans-serif;">&nbsp;<span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">PCR system</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">（</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">100μl</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
 </p>
-<table align="center" cellpadding="0" cellspacing="0">
+<table align="center" cellpadding="0" cellspacing="0" width="269">
      <tbody>
          <tr class="firstRow">
-             <td style="border: 1px solid windowtext; padding: 0px 7px;" valign="top" width="146">
+             <td style="border: 1px solid black; padding: 0px 7px;" valign="top" width="146">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Buffer</span>
                  </p>
              </td>
-             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: windowtext windowtext windowtext -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="123">
+             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="123">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10μl</span>
@@ Line 735: / Line 1,885: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">dNTPS</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10μl</span>
@@ Line 747: / Line 1,897: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">BFP&nbsp; &nbsp; fragment</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">GFP&nbsp; fragment</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
@@ Line 759: / Line 1,909: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">sJanus fragment</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">sJanus-m fragment</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="123">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
@@ Line 771: / Line 1,921: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Pfu enzyme</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1μl</span>
@@ Line 783: / Line 1,933: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">ddH2O</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">69μl</span>
@@ Line 796: / Line 1,946: @@
      </tbody>
 </table>
-<p style="margin-left: 24px; text-align: left; text-indent: 0px; line-height: 1.5em;">
-    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp; <br/></span>
-</p>
 <p style="margin-left: 24px; text-align: left; text-indent: 0px; line-height: 1.5em;">
      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Add two kinds of primer (1μl) after 5 cycle.</span>
 </p>
-<p style="text-align: center; line-height: 1.5em;">
+<p style="margin-left: 24px; text-align: center; text-indent: 0px; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">cycle</span>
 </p>
-<table align="center" cellpadding="0" cellspacing="0">
+<table align="center" cellpadding="0" cellspacing="0" width="269">
      <tbody>
          <tr class="firstRow">
-             <td style="border: 1px solid windowtext; padding: 0px 7px;" valign="top" width="136">
+             <td style="border: 1px solid black; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: windowtext windowtext windowtext -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
@@ Line 820: / Line 1,967: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
@@ Line 832: / Line 1,979: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">1</span><span style="font-size: 19px; font-family: 宋体;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">50</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">1</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">50</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">2</span><span style="font-size: 19px; font-family: 宋体;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">56</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">2</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">56</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">3</span><span style="font-size: 19px; font-family: 宋体;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">65</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">3</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">65</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
                  </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
@@ Line 853: / Line 2,045: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">1</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">50</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">2</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">56</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">3</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">65</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
@@ Line 865: / Line 2,075: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
@@ Line 877: / Line 2,087: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Forever</span>
+                </p>
+            </td>
+        </tr>
+    </tbody>
+</table>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">qualitative agarose gel electrophpresis</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">GFP</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">－</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus-m for SOE</span>　<span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR with PSB1C3 worked</span></span>
+</p>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"><img alt="demo.jpg" src="/server/ueditor/upload/image/demo.jpg" title=""/></span>
+</p>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 1000bp approximately.</span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">Electrophoresis</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">of the</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">PCR</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">products</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">was</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">identified</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">, and&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">finally</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">PCR</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">product</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">was purified by</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">gel</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">extraction</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">kit</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">to</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">prepare for</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">digestion</span>.</span></span>
+</p>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Fragment concentration: 30.5ng/μL</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date: Wednesday,7.15.2015</span></strong></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">SOE PCR</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">gene of interst</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">GFP- sJanus</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">primer</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Xba1-GFP</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">、</span></span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">inJanus-Pst1</span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">&nbsp;temperature</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;"> 55, 56.3 ,57.8 and 58.9 centigrade.</span></span>
+</p>
+<p style="margin-left: 24px; text-align: center; text-indent: 0px; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">PCR system</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">（</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">100μl</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
+</p>
+<table align="center" cellpadding="0" cellspacing="0" width="269">
+    <tbody>
+        <tr class="firstRow">
+            <td style="border: 1px solid black; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Buffer</span>
+                </p>
+            </td>
+            <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">dNTPS</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">GFP&nbsp; fragment</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">inJanus fragment</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Pfu enzyme</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">ddH2O</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">69μl</span>
+                </p>
+            </td>
+        </tr>
+    </tbody>
+</table>
+<p style="margin-left: 24px; text-align: left; text-indent: 0px; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Add two kinds of primer (1μl) after 5 cycle.</span>
+</p>
+<p style="margin-left: 24px; text-align: center; text-indent: 0px; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">cycle</span>
+</p>
+<table align="center" cellpadding="0" cellspacing="0" width="269">
+    <tbody>
+        <tr class="firstRow">
+            <td style="border: 1px solid black; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
@@ Line 889: / Line 2,230: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
@@ Line 901: / Line 2,242: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">1</span><span style="font-size: 19px; font-family: 宋体;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">50</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">1</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">55</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">2</span><span style="font-size: 19px; font-family: 宋体;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">56</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">2</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">56.3</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
-                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">3</span><span style="font-size: 19px; font-family: 宋体;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">65</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">3</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">57.8</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">4</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">58.9</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
                  </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
@@ Line 922: / Line 2,311: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">1</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">50</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">2</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">56</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">3</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">65</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
@@ Line 934: / Line 2,341: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
@@ Line 946: / Line 2,353: @@
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="136">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="132">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
                      <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Forever</span>
@@ Line 959: / Line 2,366: @@
      </tbody>
 </table>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">qualitative agarose gel electrophpresis</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">GFP</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">－</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">inJanus for SOE PCR with PSB1C3 worked</span></span>
+</p>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"><img alt="demo.jpg" src="/server/ueditor/upload/image/demo.jpg" title=""/></span>
+</p>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">（</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 1000bp approximately.</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">Electrophoresis</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">of the</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">PCR</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">products</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">was</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">identified</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">, and&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">finally</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">PCR</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">product</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">was purified by</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">gel</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">extraction</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">kit</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">to</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">prepare for</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">digestion</span>.</span></span>
+</p>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Fragment concentration:31.1ng/μL</span>
+</p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+     <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date</span></strong><strong><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span></strong><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Wednesday,7.15.2015</span></strong></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">SOE PCR</span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">3.3 Results</span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">gene of interst</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">GFP-inJanus</span></span>
 </p>
-<p style="text-align: center; line-height: 1.5em;">
+<p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"><img alt="demo.jpg" src="https://static.igem.org/mediawiki/2015/a/af/Tianjin_BFP_2.png" title=""/></span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">primer</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Xba1-GFP</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">、</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">inJanus-Pst1</span></span>
 </p>
-<p style="text-align:left">
+<p style="line-height: 1.5em;">
-     <span style="position: absolute; z-index: 251692032; margin-left: 196px; margin-top: 41px; width: 35px; height: 11px; font-family: arial,helvetica,sans-serif;"><br/></span>
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">&nbsp;temperature</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;"> 55, 56.3 ,57.8 and 58.9 centigrade.</span></span>
+</p>
+<p style="margin-left: 24px; text-align: center; text-indent: 0px; line-height: 1.5em;">
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">PCR system</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">（</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">100μl</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
+</p>
+<table align="center" cellpadding="0" cellspacing="0" width="269">
+    <tbody>
+        <tr class="firstRow">
+            <td style="border: 1px solid black; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Buffer</span>
+                </p>
+            </td>
+            <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">dNTPS</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">GFP&nbsp; fragment</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">inJanus-m fragment</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Pfu enzyme</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="146">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">ddH2O</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" valign="top" width="123">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">69μl</span>
+                </p>
+            </td>
+        </tr>
+    </tbody>
+</table>
+<p style="margin-left: 24px; text-align: left; text-indent: 0px; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Add two kinds of primer (1μl) after 5 cycle.</span>
+</p>
+<p style="margin-left: 24px; text-align: center; text-indent: 0px; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">cycle</span>
+</p>
+<table align="center" cellpadding="0" cellspacing="0" width="269">
+    <tbody>
+        <tr class="firstRow">
+            <td style="border: 1px solid black; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">1</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">55</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">2</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">56.3</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">3</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">57.8</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">4</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">58.9</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">1</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">50</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">2</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">56</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">3</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">65</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; text-indent: 0px; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Forever</span>
+                </p>
+            </td>
+        </tr>
+    </tbody>
+</table>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;">&nbsp;<span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">qualitative agarose gel electrophpresis</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">GFP</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">－</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">inJanus-m for SOE</span>　<span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR with PSB1C3 worked</span></span>
 </p>
 <p style="text-align: center; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Figure 2</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"><img alt="demo.jpg" src="/server/ueditor/upload/image/demo.jpg" title=""/></span>
+</p>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">（</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 1000bp approximately.</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">Electrophoresis</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">of the</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">PCR</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">products</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">was</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">identified</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">, and&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">finally</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">PCR</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">product</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">was purified by</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">gel</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">extraction</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">kit</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">to</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">prepare for</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">digestion</span>.</span></span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Fragment concentration:50.3ng/μL</span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;">&nbsp;<strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date: Thursday,7.16.2015</span></strong></span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">restriction enzyme digestion</span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">After qualitative agarose gel electrophpresis, all the target sequence are 1000bp approximately.</span>
 </p>
 <p style="text-align: center; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"><img alt="demo.jpg" src="https://static.igem.org/mediawiki/2015/e/e9/Tianjin_BFP_3.png" title=""/></span>
+    <img alt="demo.jpg" src="/server/ueditor/upload/image/demo.jpg" title=""/>
+</p>
+<p style="line-height: 1.5em;">
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"></span><br/>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Fragment concentration: sJanus is 6.9ng/μL, sJanus-m is 15.3ng/μL, inJanus is 4.6ng/μL, inJanus-m is 7.0ng/μL.</span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date: Friday,7.17.2015</span></strong></span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">We used T4 DNA ligase to connect them at the temperature of 22 centigrade about an hour. </span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Figure 3. The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 1000bp approximately. We failed at first attempt. But as we change the temperature we use in PCR cycle. We finally get the target fragment. </span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Transformed the expression vectors we had already connected into the <em>Escherichia coli </em>DH5α, then incubated the bacteria for 12h (37</span><span style="font-size: 19px; font-family: 宋体;">℃</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">).</span></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">As we can see from the picture, there are two bands in every channel. One is about 1000bp and another is about 300bp. We do not know the reason of this. But after doing the confirmatory experiment, we are sure the 1000bp band is the target band we want (BFP- sJanus &amp; BFP-sJanus M). We did not get the fragment at first attempt. We change the temperature in PCR cycle. </span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Date:Saturday,7.18.2015</span>
+</p>
+<p style="text-align: center; line-height: 1.5em;">
+    <img alt="demo.jpg" src="/server/ueditor/upload/image/demo.jpg" title=""/>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"></span><br/>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Enzymes cut each, but they did not work.</span>
+</p>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">（</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">The maximum of marker is 8000bp, and following is 8000, 5000, 3000,1500,1000and 500bp from up and down, our target sequence is about 1000bp approximately. But the bands in this figure are all about 5000bp, so we suspect that the fragments are not connected to plasmid.</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Analyse</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">The fragments are not connected to plasmid.</span></span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date: Sunday,7.19.2015</span></strong></span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">We chose another DH5α and trans</span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">formed again.</span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date: Monday,7.20.2015</span></strong></span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Enzyme cut, </span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date: Tuesday,7.21.2015</span></strong></span>
+</p>
+<p style="line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">We design another kind of primer and reverse which contain EcoRI and XhoI site. We have got the standard GFP-sJanus and GFP-sJanus-m fragments, and we got the target fragments used to construct the expression vector by PCR.</span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">4. Construct the plasmid</span></strong></span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">gene of interst</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">EcoR1-GFP-sJanus -Xho1</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">、</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">EcoR1-GFP-sJanus-m-Xho1</span></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">4.1 We design another kind of primer and reverse which contain EcoRI and Hind III site. The condition to amplify it by PCR is same as above all. We choose pET28a(+) as our expression vector. And then, we use enzymes of EcoRI site and XhoI site to digest the PCR product and plasmid. We use T4 DNA ligase to connect them at the temperature of 22 centigrade. We insert the plasmid into competent DH5α. It has been confirmed that these sequences we get are right when detecting them in company. </span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">primer</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">EcoR1-GFP</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">、</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">sJanus-Xho1</span></span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">4.2 detail of the experiment</span>
+     <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">EcoR1-GFP: 5’-CGGAATTCATGCGTAAAGGAGAAGAACTT</span>
 </p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">sJanus-Xho1: 5’-CCGCTCGAGTCAAGCACCGACGGCC</span>
 </p>
 <p style="text-align: center; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Digest (60μl)&nbsp;&nbsp;&nbsp; 1h</span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">PCR system</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：（</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">50μl</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
 </p>
-<table align="center" cellpadding="0" cellspacing="0">
+<table align="center" cellpadding="0" cellspacing="0" width="269">
      <tbody>
-         <tr class="firstRow" style=";height:19px">
+         <tr class="firstRow">
-             <td style="border: 1px solid windowtext; padding: 0px 7px;" height="19" valign="top" width="141">
+             <td style="border: 1px solid black; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">ddH2O</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Buffer</span>
                  </p>
              </td>
-             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: windowtext windowtext windowtext -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" height="19" valign="top" width="137">
+             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">31μl</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
                  </p>
              </td>
          </tr>
-         <tr style=";height:19px">
+         <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" height="19" valign="top" width="141">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">buffer</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">dNTPS</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" height="19" valign="top" width="137">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">6μl</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5μl</span>
                  </p>
              </td>
          </tr>
-         <tr style=";height:19px">
+         <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" height="19" valign="top" width="141">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Fd1</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">EcoR1-GFP primer</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" height="19" valign="top" width="137">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1.5μl</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
                  </p>
              </td>
          </tr>
-         <tr style=";height:19px">
+         <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" height="19" valign="top" width="141">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Fd2</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">sJanus-Xho1 primer</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" height="19" valign="top" width="137">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1.5μl</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
                  </p>
              </td>
          </tr>
-         <tr style=";height:19px">
+         <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" height="19" valign="top" width="141">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">DNA</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Xba1-GFP-sJanus -Pst1/ Xba1-GFP-sJanus-m-Pst1</span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" height="19" valign="top" width="137">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">20μl</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">1μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Pfu enzyme</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">0.5μl</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">ddH2O</span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">34.5μl</span>
                  </p>
              </td>
@@ Line 1,066: / Line 2,817: @@
 </table>
 <p style="text-align: center; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">cycle</span>
-</p>
-<p style="text-align: left; line-height: 1.5em;">
-    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
-</p>
-<p style="text-align: center; line-height: 1.5em;">
-    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">T4 DNA ligase to connect</span><span style="font-size: 19px; font-family: 宋体;">（</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">20μl</span><span style="font-size: 19px; font-family: 宋体;">）</span><span style="font-family: arial,helvetica,sans-serif; font-size: 19px;">&nbsp; 1h</span></span>
 </p>
-<table align="center" cellpadding="0" cellspacing="0">
+<table align="center" cellpadding="0" cellspacing="0" width="269">
      <tbody>
          <tr class="firstRow">
-             <td style="border: 1px solid windowtext; padding: 0px 7px;" valign="top" width="123">
+             <td style="border: 1px solid black; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Buffer</span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: windowtext windowtext windowtext -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="100">
+             <td style="border-width: 1px 1px 1px medium; border-style: solid solid solid none; border-color: black black black -moz-use-text-color; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2μl</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5min</span>
                  </p>
              </td>
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="123">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">T4 DNA ligase</span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">95</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="100">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">0.5μl</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
                  </p>
              </td>
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="123">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">plasmid</span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">1:55.4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">2:56.6</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">3:57.8</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">4:60.3</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="100">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">X</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">30s</span>
                  </p>
              </td>
          </tr>
          <tr>
-             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color windowtext windowtext; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" valign="top" width="123">
+             <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">fragment</span>
+                     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
                  </p>
              </td>
-             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color windowtext windowtext -moz-use-text-color; padding: 0px 7px;" valign="top" width="100">
+             <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
                  <p style="text-align: center; line-height: 1.5em;">
-                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">5X</span>
+                     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">2min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">72</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">10min</span>
+                </p>
+            </td>
+        </tr>
+        <tr>
+            <td style="border-width: medium 1px 1px; border-style: none solid solid; border-color: -moz-use-text-color black black; -moz-border-top-colors: none; -moz-border-right-colors: none; -moz-border-bottom-colors: none; -moz-border-left-colors: none; border-image: none; padding: 0px 7px;" align="center" valign="middle" width="136">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">4</span><span style="font-size: 19px; font-family: 宋体;">℃</span></span>
+                </p>
+            </td>
+            <td style="border-width: medium 1px 1px medium; border-style: none solid solid none; border-color: -moz-use-text-color black black -moz-use-text-color; padding: 0px 7px;" align="center" valign="middle" width="132">
+                <p style="text-align: center; line-height: 1.5em;">
+                    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">Forever</span>
                  </p>
              </td>
@@ Line 1,126: / Line 2,904: @@
      </tbody>
 </table>
+<p style="margin-left: 14px; text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">qualitative agarose gel electrophpresis</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">：</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">fragments on these four centigrades all worked well.</span></span>
+</p>
+<p style="text-align: center; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;"><img alt="demo.jpg" src="/server/ueditor/upload/image/demo.jpg" title=""/></span>
+</p>
 <p style="text-align: left; line-height: 1.5em;">
-     <span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">&nbsp;</span>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">（</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">The maximum of marker is 2000bp, and following is 1000, 750, 500, 250 and 100bp from up and down, our target sequence is about 1000bp approximately.</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">）</span></span>
 </p>
-<p>
+<p style="line-height: 1.5em;">
-     <br/>
+     <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">Electrophoresis</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">of the</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">PCR</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">products</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">was</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">&nbsp;</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext; border: 1px none windowtext; padding: 0px;">identified</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif; color: windowtext;">, and&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">finally</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">PCR</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">product</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">was purified by</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">gel</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">extraction</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">kit</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">to</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">prepare for</span>&nbsp;<span style="font-family: arial,helvetica,sans-serif; border: 1px none windowtext; padding: 0px;">digestion</span>.</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Fragment concentration:EcoR1-GFP-sJanus -Xho1:55.0ng/μL</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">、</span></span><span style="font-size: 19px; font-family: arial,helvetica,sans-serif;">EcoR1-GFP-sJanus-m-Xho1:60ng/μL</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date: Saturday,8.1.2015</span></strong></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">We used enzymes of EcoRI site and XhoI site to digest the PCR product and plasmid. We used T4 DNA ligase to connect them at the temperature of 22 centigrade for about one hour. Then we transformed the expression vectors we had already connected into the <em>Escherichia coli </em>DH5α, then incubated the bacteria for 12h (37</span><span style="font-size: 19px; font-family: 宋体;">℃</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">)</span></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">It has been confirmed that these sequences we get are right when detecting them in company.</span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><strong><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Date:Monday,8.10.2015</span></strong></span>
+</p>
+<p style="text-align: left; line-height: 1.5em;">
+    <span style="font-family: arial,helvetica,sans-serif;"><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">Transformed the expression vectors(concentration:140.3ng/μL</span><span style="font-size: 19px; font-family: &quot;Arial Unicode MS&quot;,sans-serif;">、</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">121.4ng/μL) we had already built before (pET28a(+)) into the <em>Escherichia coli </em>BL21, then incubated the bacteria for 12h (37</span><span style="font-size: 19px; font-family: 宋体;">℃</span><span style="font-size: 19px; font-family: &quot;Calibri&quot;,sans-serif;">).</span></span>
 </p>
 </div>