Difference between revisions of "Team:Northeastern Boston/BasicParts"

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        <a>Basic & Composite</a>
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               <h1>Basic and Composite Parts</h1>
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               <h1 id="project_header">BASIC & COMPOSITE PARTS</h1>
  
 
<h2>Hygromycin B Resistance Cassette</h2>
 
<h2>Hygromycin B Resistance Cassette</h2>

Revision as of 04:19, 14 September 2015

BASIC & COMPOSITE PARTS

Hygromycin B Resistance Cassette

Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from pOpt_mVenus_Hyg and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transfected with this part.

This part contains intron 2 of RBCs2 after a fusion HSP70A-RBCs2 promoter for greater expression.[1]