Difference between revisions of "Team:Northeastern Boston/BasicParts"
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<h2>Hygromycin B Resistance Cassette</h2> | <h2>Hygromycin B Resistance Cassette</h2> | ||
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<p>Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from <a href="http://chlamycollection.org/plasmid/popt_mvenus_hyg" target="_blank">pOpt_mVenus_Hyg</a> and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transfected with this part. </p> | <p>Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from <a href="http://chlamycollection.org/plasmid/popt_mvenus_hyg" target="_blank">pOpt_mVenus_Hyg</a> and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transfected with this part. </p> | ||
Revision as of 04:30, 14 September 2015
BASIC & COMPOSITE PARTS
Hygromycin B Resistance Cassette
Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from pOpt_mVenus_Hyg and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transfected with this part.
This part contains intron 2 of RBCs2 after a fusion HSP70A-RBCs2 promoter for greater expression.[1]