Difference between revisions of "Team:Northeastern Boston/BasicParts"
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<p>Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from <a href="http://chlamycollection.org/plasmid/popt_mvenus_hyg" target="_blank">pOpt_mVenus_Hyg</a> and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transfected with this part. </p> | <p>Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from <a href="http://chlamycollection.org/plasmid/popt_mvenus_hyg" target="_blank">pOpt_mVenus_Hyg</a> and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transfected with this part. </p> | ||
| − | <p>This part contains intron 2 of RBCs2 after a fusion HSP70A-RBCs2 promoter for greater expression.[<a href="http://onlinelibrary.wiley.com/store/10.1046/j.1365-313x.2000.00652.x/asset/j.1365-313x.2000.00652.x.pdf?v=1&t=iejf1jm1&s=f3a9125d087876eeeb8fe8892916ac1a9a96c3a9" target="_blank">1</a>] | + | <p>This part contains intron 2 of RBCs2 after a fusion HSP70A-RBCs2 promoter for greater expression. [<a href="http://onlinelibrary.wiley.com/store/10.1046/j.1365-313x.2000.00652.x/asset/j.1365-313x.2000.00652.x.pdf?v=1&t=iejf1jm1&s=f3a9125d087876eeeb8fe8892916ac1a9a96c3a9" target="_blank">1</a>] Aph7'', the gene product, confers resistance to Hygromycin B, at antibiotic concentrations up to 200g/mL. [<a href="http://www.sciencedirect.com/science/article/pii/S1434461004701170" target="_blank">2</>]</p> |
Revision as of 04:41, 14 September 2015
BASIC & COMPOSITE PARTS
Hygromycin B Resistance Cassette
Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from pOpt_mVenus_Hyg and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transfected with this part.
This part contains intron 2 of RBCs2 after a fusion HSP70A-RBCs2 promoter for greater expression. [1] Aph7'', the gene product, confers resistance to Hygromycin B, at antibiotic concentrations up to 200g/mL. [2]