Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w8"
(→pdark natural transformation) |
(→pcpcG2 electro-transformation) |
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====pcpcG2 electro-transformation==== | ====pcpcG2 electro-transformation==== | ||
| + | '''in the evening of 24th, Aug''' | ||
| + | We picked 4 colonies (marked with 1,2,3 & 4) from the pcpcG2-HR plate and spread them on another BG11 plate in parallel seprately to do solid segregation | ||
| + | |||
| + | '''in the evening of 30th, Aug''' | ||
| + | We scraped the grown cell from the plate, which is 2,3 & 4 to do colony PCR, 1st liquid segregation and solid storage | ||
====pre-experiment of pdark-HR==== | ====pre-experiment of pdark-HR==== | ||
Revision as of 07:14, 14 September 2015
Contents
Transformation
pdark natural transformation
in the evening of 29th, Aug
2nd liquid segregation of pdark-HR
pcpcG2 electro-transformation
in the evening of 24th, Aug We picked 4 colonies (marked with 1,2,3 & 4) from the pcpcG2-HR plate and spread them on another BG11 plate in parallel seprately to do solid segregation
in the evening of 30th, Aug We scraped the grown cell from the plate, which is 2,3 & 4 to do colony PCR, 1st liquid segregation and solid storage