Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w8"
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====pre-experiment of pdark-HR==== | ====pre-experiment of pdark-HR==== | ||
| + | '''on 24th, Aug''' | ||
| + | |||
| + | Took the third 4 samples at 9am & fourth 4 samples at 9pm. | ||
| + | |||
| + | '''on 25th, Aug''' | ||
| + | |||
| + | Took the fifth 4 samples at 9am & sixth 4 samples at 9pm. | ||
| + | |||
| + | '''on 26th, Aug''' | ||
| + | |||
| + | Took the seventh 4 samples at 9am & eighth 4 samples at 9pm. | ||
====pdark-GFP electro-transformation==== | ====pdark-GFP electro-transformation==== | ||
<html></div></html> | <html></div></html> | ||
Revision as of 07:15, 14 September 2015
Contents
Transformation
pdark natural transformation
in the evening of 29th, Aug
2nd liquid segregation of pdark-HR
pcpcG2 electro-transformation
in the evening of 24th, Aug We picked 4 colonies (marked with 1,2,3 & 4) from the pcpcG2-HR plate and spread them on another BG11 plate in parallel seprately to do solid segregation
in the evening of 30th, Aug We scraped the grown cell from the plate, which is 2,3 & 4 to do colony PCR, 1st liquid segregation and solid storage
pre-experiment of pdark-HR
on 24th, Aug
Took the third 4 samples at 9am & fourth 4 samples at 9pm.
on 25th, Aug
Took the fifth 4 samples at 9am & sixth 4 samples at 9pm.
on 26th, Aug
Took the seventh 4 samples at 9am & eighth 4 samples at 9pm.