Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w9"
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====second desalination test of pdark-HR==== | ====second desalination test of pdark-HR==== | ||
| + | '''on 2nd, Sep''' | ||
| + | |||
| + | inoculated 24 wildtype & 24 pdark-HR (use pdark-HR from colony 3) at 9pm | ||
| + | |||
| + | '''on 4th, Sep''' | ||
| + | |||
| + | induced with darkness at 9pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each) | ||
| + | |||
| + | '''on 5th, Sep''' | ||
| + | |||
| + | cultivated with white light at 9am, then took the second 6 samples, | ||
| + | |||
| + | |||
| + | took the third 6 samples at 10am | ||
| + | |||
| + | |||
| + | took the fourth 6 samples at 11am | ||
| + | |||
| + | |||
| + | took the fifth 6 samples at 1pm | ||
| + | |||
| + | |||
| + | took the sixth 6 samples at 9pm | ||
| + | |||
| + | '''on 6th, Sep''' | ||
| + | |||
| + | took the seventh 6 samples at 9am | ||
| + | |||
| + | |||
| + | took the eighth 6 samples at 9pm | ||
| + | |||
| + | |||
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Revision as of 07:24, 14 September 2015
Contents
Transformation
pdark natural transformation
in the evening of 31st, Aug
3rd liquid segregation of pdark-HR
in the evening of 3rd, Sep
4th liquid segregation of pdark-HR
in the evening of 6th, Sep
5th liquid segregation of pdark-HR
pcpcG2 electro-transformation
in the evening of 2nd, Sep
2nd liquid segregation of pcpcG2-HR
in the evening of 4th, Sep
3rd liquid segregation of pcpcG2-HR
in the evening of 6th, Sep
4th liquid segregation of pcpcG2-HR
second desalination test of pdark-HR
on 2nd, Sep
inoculated 24 wildtype & 24 pdark-HR (use pdark-HR from colony 3) at 9pm
on 4th, Sep
induced with darkness at 9pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each)
on 5th, Sep
cultivated with white light at 9am, then took the second 6 samples,
took the third 6 samples at 10am
took the fourth 6 samples at 11am
took the fifth 6 samples at 1pm
took the sixth 6 samples at 9pm
on 6th, Sep
took the seventh 6 samples at 9am
took the eighth 6 samples at 9pm