Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w10"
(→first desalination test of pcpcG2-HR) |
(→first desalination test of pcpcG2-HR) |
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| Line 13: | Line 13: | ||
'''on 7th, Sep''' | '''on 7th, Sep''' | ||
| − | Inoculated 15 wildtype & 15 pcpcG2-HR (use pcpcG2-HR from colony 2) at 10pm | + | Inoculated 15 wildtype & 15 pcpcG2-HR (use pcpcG2-HR from colony 2) at 10pm. |
'''on 9th, Sep''' | '''on 9th, Sep''' | ||
| − | Induced with green light at 10pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each) | + | Induced with green light at 10pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each). |
'''on 10th, Sep''' | '''on 10th, Sep''' | ||
| − | + | Cultivated with red light at 10am, then took the second 6 samples, | |
| − | + | Took the third 6 samples at 2pm. | |
| − | + | Took the fourth 6 samples at 10pm. | |
'''on 11th, Sep''' | '''on 11th, Sep''' | ||
| − | + | Took the fifth 6 samples at 10am. | |
===pdark-GFP electro-transformation=== | ===pdark-GFP electro-transformation=== | ||
Revision as of 08:45, 14 September 2015
Contents
Transformation
pdark natural transformation
in the evening of 11th, Sep
6th liquid segregation of pdark-HR
pcpcG2 electro-transformation
in the evening of 11th Sep
5th liquid segregation of pcpcG2-HR
first desalination test of pcpcG2-HR
on 7th, Sep
Inoculated 15 wildtype & 15 pcpcG2-HR (use pcpcG2-HR from colony 2) at 10pm.
on 9th, Sep
Induced with green light at 10pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each).
on 10th, Sep
Cultivated with red light at 10am, then took the second 6 samples,
Took the third 6 samples at 2pm.
Took the fourth 6 samples at 10pm.
on 11th, Sep
Took the fifth 6 samples at 10am.
pdark-GFP electro-transformation
on 8th, Sep
We picked 1 colony (only 1 colony appeared) from the pdark-GFP plate and spread them on another BG11 plate in line to do solid segregation