Difference between revisions of "Team:ZJU-China/Safety"

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<h2>Safety in iGEM</h2>
 
  
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h4>Safe Project Design</h4>
 
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
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<ul>
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<body id = "wrapper">
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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<h4>Safe Lab Work</h4>
 
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<div id="page-content-wrapper">
  
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<h4>Safe Shipment</h4>
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<article>
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    <div class="link" id="Safety"></div>
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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    <h2>Safety</h2>
 +
    <div class="row">
 +
<h3 style="border-bottom:1px solid #555;font-size:2em;text-align:center;padding-bottom:10px" >  Lab Safety </h3>
 +
        <div class="col-md-6 text-col-left">
 +
         
 +
            <p class="p1">• Lab Safety Training</p>
 +
          <p class="p2">
 +
          Before our team members were allowed to enter the lab, we must all take the lab safety training. The training includes how to use the machine in our lab properly, how to react correctly if there was an emergency and how to protect ourselves during the experiments.
 +
</p>
 +
<p class="p1">
 +
• Safety during the experiments
 +
</p>
 +
<p class="p2">
 +
When we are doing the experiments, we can guarantee that:
 +
</p>
 +
<ul type="square">
 +
<li>
 +
Wearing lab-grown
 +
</li>
 +
<li>
 +
Wearing lab gloves
 +
</li>
 +
<li>
 +
Using the experimental machine properly
 +
</p>
 +
<li>
 +
Deal with the waste in a proper manner
 +
</li>
 +
</ul>
 +
<p class="p1">
 +
• Safety tutor
 +
</p>
 +
<p class="p2">
 +
We have a safety tutor in our lab. He taught us how to do the experiment safely, and he will watch us to make sure that we all behave in the right way. When we do something that is unsafe, he will point out and make sure that we won’t do it again.
 +
</p>
 +
        </div>
 +
        <div class="col-md-6 img-100">
 +
                   
 +
            <img src="https://static.igem.org/mediawiki/2015/5/5c/ZJU-China_Safety1.jpg" class="img-center"></img>
 +
            <div class="cpright">
 +
            <p class="kuvateksti">
 +
             
 +
            </p>
 +
            </div>
 +
            <img src="https://static.igem.org/mediawiki/2015/f/f5/ZJU-China_Safety2.jpg" class="img-center"></img>
 +
            <div class="cpright">
 +
            <p class="kuvateksti">
 +
               
 +
            </p>
 +
            </div>
 +
        </div>
 +
    </div>
  
 +
 +
<h3 style="border-bottom:1px solid #555;font-size:2em;text-align:center;padding-bottom:10px" > Biobrick Safety </h3>
 +
 +
    <div class="row">
 +
           
 +
            <p class="p1 textcenter">
 +
The Biobrick parts we built have no safety issue according to our knowledge.
 +
            </p>
 +
  </div>
 +
 +
 +
        <h3 style="border-bottom:1px solid #555;font-size:2em;text-align:center;padding-bottom:10px" >Project Related Material Safety</h3>
 +
    <div class="row">
 +
     
 +
         
 +
            <p class="p1">
 +
① Avermectins
 +
            </p>
 +
 +
<div class="col-md-6 text-col-left">
 +
            <p class="p1">
 +
• Environmental Safety
 +
            </p>
 +
<ul type="square">
 +
            <li>
 +
Can be decomposed easily by microorganism in soil
 +
            </li>
 +
            <li>
 +
Sensitive to sun light
 +
            </li>
 +
            <li>
 +
High selectivity: only have effects on a few certain pests including termites and cotton aphid
 +
            </li>
 +
            <li>
 +
Complete safe to large mammals under recommended concentrations
 +
            </li>
 +
</ul>
 +
</div>
 +
 +
 +
<div class="col-md-6 text-col-right">
 +
            <p class="p1">
 +
• Application Safety
 +
            </p>
 +
<ul type="square">
 +
            <li>
 +
Complete safe to users under recommended concentration
 +
            </li>
 +
            <li>
 +
Already existed mature industrial production system
 +
            </li>
 +
            <li>
 +
Already have more than 3000 registered avermetin-related products in China, passing the security check of AQSIQ (State General Administration of the People’s Republic of China for Quality Supervision and Inspection and Quarantine)
 +
            </li>
 +
</ul>
 +
</div>
 +
 +
 +
            <p class="p1" style="padding-top:20px;">
 +
②Toxic Proteins
 +
            </p>
 +
       
 +
         
 +
  <div class="col-md-6 text-col-left">
 +
               
 +
            <img src="https://static.igem.org/mediawiki/2015/e/e5/ZJU-China_Safety3.png" class="img-center"></img>
 +
            <div class="cpright">
 +
            <p class="kuvateksti">
 +
         
 +
            </p>
 +
      </div>
 +
</div>
 +
 +
 +
<div class="col-md-6 text-col-right textcenter">
 +
            <img src="https://static.igem.org/mediawiki/2015/0/0b/ZJU-China_Background2.jpg" class="img-center" style="width:auto;height:347px"></img>
 +
            <div class="cpright">
 +
            <p class="kuvateksti">
 +
             
 +
             
 +
            </p>
 +
      </div>
 +
        </div>
 +
 +
 +
  
 
</div>
 
</div>
 +
 
 +
  <h3 style="border-bottom:1px solid #555;font-size:2em;text-align:center;padding-bottom:10px" >Experimental Safety</h3>
 +
    <div class="row">
 +
        <div>
 +
         
 +
            <p class="p1">
 +
            During the experiment, we worry that our gene modified bacterium would get out of the lab, thus we figured out several ways to make our bacterium safer.
 +
            </p>
 +
 +
            <p class="p1" style="padding-top:20px">
 +
            <b>① Inducible Promoter</b>
 +
            </p>
 +
            <p class="p1">
 +
            In our plan, we shall build four constructs to express toxin proteins that helps killing termites. In case that our bacterium get outside and leak the toxic protein into the environment, we use an inducible promoter in our constructs. Only when there is arabinose in the medium, can the promoter work as usual. There is no arabinose outside the lab, so our bacterium won’t leak the toxic protein.
 +
            </p>
 +
            <p class="p1">
 +
            <b>②Kill Switch</b>
 +
            </p>
 +
            <p class="p1">
 +
            With regard to this CidA-LrgA system and the BNU iGEM 2014 kill switch design6, we designed our own kill switch as the figure 3 shows. When cultured with our IPTG-added medium, these bacteria would live as usual to function as a mini-factory to amplify plasmids or to produce plasmids for our use, whereas they would die due to the cell membrane lysis and release of genomic DNA. Moreover, we put the lrgA gene under the control of a weak and constitutive promoter J23116 to in case of the leaky expression of the CidA to prevent cell lysis.
 +
            </p>
 +
<div class="col-md-12 img-100 textcenter">
 +
            <img src="https://static.igem.org/mediawiki/2015/d/de/ZJU-China_Safety5.jpg"  class="img-center" style="width:80%"></img>
 +
            <div class="cpleft">
 +
            <p class="kuvateksti">             
 +
            </p>
 +
      </div>
 +
</div>
 +
        </div>
 +
  </div>
 +
 +
 +
 +
 +
<h3 style="border-bottom:1px solid #555;font-size:2em;text-align:center;padding-bottom:10px" >Device Safety & Social Safety</h3>
 +
 +
    <div class="row">
 +
        <div>
 +
       
 +
            <p class="p1">
 +
In our project, we designed a device aiming at better controlling and killng termites. This device is absolutely not allowed to be used outdoors, but is designed to be used in the house instead. After we designed the device, we are afraid that people might use it in a wrong way after they bought it. In order to avoid people wrongly operating our device, we also design an instruction of our device as below.
 +
            </p>
 +
            <img src="???" class="img-center"></img>
 +
            <div class="cpleft">
 +
            <p class="kuvateksti">
 +
                Fig 6
 +
            </p>
 +
    </div>
 +
            <p class="p1">
 +
We plan to sell our device with the instruction attached to it, and we believe that our device would be safer with our well-designed instruction.
 +
            </p>
 +
            <p class="p1">
 +
Furthermore, we considered the social safety of our whole project. According to the chart we show below, we considered every aspect to confirm that our project are safe under given conditions..
 +
            </p>
 +
            <img src="???" class="img-center"></img>
 +
            <div class="cpleft">
 +
            <p class="kuvateksti">
 +
                Fig7
 +
            </p>
 +
    </div>
 +
        </div>
 +
  </div>
 +
</article>
 +
 +
</div>
 +
 +
 +
 +
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{{ZJU-China/mascot}}
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{{:Team:ZJU-China/template/foot}}

Revision as of 13:05, 14 September 2015

、

Safety

Lab Safety

• Lab Safety Training

Before our team members were allowed to enter the lab, we must all take the lab safety training. The training includes how to use the machine in our lab properly, how to react correctly if there was an emergency and how to protect ourselves during the experiments.

• Safety during the experiments

When we are doing the experiments, we can guarantee that:

  • Wearing lab-grown
  • Wearing lab gloves
  • Using the experimental machine properly

  • Deal with the waste in a proper manner

• Safety tutor

We have a safety tutor in our lab. He taught us how to do the experiment safely, and he will watch us to make sure that we all behave in the right way. When we do something that is unsafe, he will point out and make sure that we won’t do it again.

Biobrick Safety

The Biobrick parts we built have no safety issue according to our knowledge.

Project Related Material Safety

① Avermectins

• Environmental Safety

  • Can be decomposed easily by microorganism in soil
  • Sensitive to sun light
  • High selectivity: only have effects on a few certain pests including termites and cotton aphid
  • Complete safe to large mammals under recommended concentrations

• Application Safety

  • Complete safe to users under recommended concentration
  • Already existed mature industrial production system
  • Already have more than 3000 registered avermetin-related products in China, passing the security check of AQSIQ (State General Administration of the People’s Republic of China for Quality Supervision and Inspection and Quarantine)

②Toxic Proteins

Experimental Safety

During the experiment, we worry that our gene modified bacterium would get out of the lab, thus we figured out several ways to make our bacterium safer.

① Inducible Promoter

In our plan, we shall build four constructs to express toxin proteins that helps killing termites. In case that our bacterium get outside and leak the toxic protein into the environment, we use an inducible promoter in our constructs. Only when there is arabinose in the medium, can the promoter work as usual. There is no arabinose outside the lab, so our bacterium won’t leak the toxic protein.

②Kill Switch

With regard to this CidA-LrgA system and the BNU iGEM 2014 kill switch design6, we designed our own kill switch as the figure 3 shows. When cultured with our IPTG-added medium, these bacteria would live as usual to function as a mini-factory to amplify plasmids or to produce plasmids for our use, whereas they would die due to the cell membrane lysis and release of genomic DNA. Moreover, we put the lrgA gene under the control of a weak and constitutive promoter J23116 to in case of the leaky expression of the CidA to prevent cell lysis.

Device Safety & Social Safety

In our project, we designed a device aiming at better controlling and killng termites. This device is absolutely not allowed to be used outdoors, but is designed to be used in the house instead. After we designed the device, we are afraid that people might use it in a wrong way after they bought it. In order to avoid people wrongly operating our device, we also design an instruction of our device as below.

Fig 6

We plan to sell our device with the instruction attached to it, and we believe that our device would be safer with our well-designed instruction.

Furthermore, we considered the social safety of our whole project. According to the chart we show below, we considered every aspect to confirm that our project are safe under given conditions..

Fig7

termit