Difference between revisions of "Team:TU Dresden/Notebook/PlasmidDesign"

Line 56: Line 56:
 
<h3>Plasmid Maps</h3>
 
<h3>Plasmid Maps</h3>
 
<h4>M13 gene 3 coding for infection protein P3</h4>
 
<h4>M13 gene 3 coding for infection protein P3</h4>
−
For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/b/b3/TU_Dresden_geneIII_first.pdf"><font color="#045FB4">here</font></a>
+
<p style="line-height:1.8">For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/b/b3/TU_Dresden_geneIII_first.pdf"><font color="#045FB4">here</font></a></p>
 
<figure align="center">
 
<figure align="center">
 
<a href="https://static.igem.org/mediawiki/2015/e/e8/TU_Dresden_geneIII_first_cut.jpg" title="View">
 
<a href="https://static.igem.org/mediawiki/2015/e/e8/TU_Dresden_geneIII_first_cut.jpg" title="View">
Line 63: Line 63:
  
 
<h4>HER2 (Human Epidermal Growth Factor 2) cytoplasmic domain</h4>
 
<h4>HER2 (Human Epidermal Growth Factor 2) cytoplasmic domain</h4>
−
For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/3/3a/TU_Dresden_her2_first.pdf"><font color="#045FB4">here</font></a>
+
<p style="line-height:1.8">For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/3/3a/TU_Dresden_her2_first.pdf"><font color="#045FB4">here</font></a></p>
 
<figure align="center">
 
<figure align="center">
 
<a href="https://static.igem.org/mediawiki/2015/3/3a/TU_Dresden_her2_first.pdf" title="View">
 
<a href="https://static.igem.org/mediawiki/2015/3/3a/TU_Dresden_her2_first.pdf" title="View">
Line 70: Line 70:
  
 
<h4>ZHER2 - affibody with affinity to HER2</h4>
 
<h4>ZHER2 - affibody with affinity to HER2</h4>
−
For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/d/d9/TU_Dresden_zher2_first.pdf"><font color="#045FB4">here</font></a>
+
<p style="line-height:1.8">For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/d/d9/TU_Dresden_zher2_first.pdf"><font color="#045FB4">here</font></a></p>
 
<figure align="center">
 
<figure align="center">
 
<a href="https://static.igem.org/mediawiki/2015/e/e0/TU_Dresden_zher2_affibody_first_cut.jpg" title="View">
 
<a href="https://static.igem.org/mediawiki/2015/e/e0/TU_Dresden_zher2_affibody_first_cut.jpg" title="View">
Line 77: Line 77:
  
 
<h4>T25 - subunit of BACTH system</h4>
 
<h4>T25 - subunit of BACTH system</h4>
−
For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/2/26/TU_Dresden_t25_first.pdf"><font color="#045FB4">here</font></a>
+
<p style="line-height:1.8">For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/2/26/TU_Dresden_t25_first.pdf"><font color="#045FB4">here</font></a></p>
 
<figure align="center">
 
<figure align="center">
 
<a href="https://static.igem.org/mediawiki/2015/7/75/TU_Dresden_t25_bacth_first_cut.jpg" title="View">
 
<a href="https://static.igem.org/mediawiki/2015/7/75/TU_Dresden_t25_bacth_first_cut.jpg" title="View">
Line 84: Line 84:
  
 
<h4>T18 - subunit of BACTH system</h4>
 
<h4>T18 - subunit of BACTH system</h4>
−
For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/3/38/TU_Dresden_t18_first.pdf"><font color="#045FB4">here</font></a>
+
<p style="line-height:1.8">For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/3/38/TU_Dresden_t18_first.pdf"><font color="#045FB4">here</font></a></p>
 
<figure align="center">
 
<figure align="center">
 
<a href="https://static.igem.org/mediawiki/2015/0/0b/TU_Dresden_t18_bacth_first_cut.jpg" title="View">
 
<a href="https://static.igem.org/mediawiki/2015/0/0b/TU_Dresden_t18_bacth_first_cut.jpg" title="View">
Line 92: Line 92:
  
 
<h4>Leucine zipper able to bind to T25 BACTH subunit</h4>
 
<h4>Leucine zipper able to bind to T25 BACTH subunit</h4>
−
For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/5/57/TU_Dresden_lzt25_first.pdf"><font color="#045FB4">here</font></a>
+
<p style="line-height:1.8">For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/5/57/TU_Dresden_lzt25_first.pdf"><font color="#045FB4">here</font></a></p>
 
<figure align="center">
 
<figure align="center">
 
<a href="https://static.igem.org/mediawiki/2015/a/a3/TU_Dresden_lzt25_bacth_first_cut.jpg" title="View">
 
<a href="https://static.igem.org/mediawiki/2015/a/a3/TU_Dresden_lzt25_bacth_first_cut.jpg" title="View">
Line 99: Line 99:
  
 
<h4>Leucine zipper able to bind to T18 BACTH subunit</h4>
 
<h4>Leucine zipper able to bind to T18 BACTH subunit</h4>
−
For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/1/1f/TU_Dresden_lzt18_first.pdf"><font color="#045FB4">here</font></a>
+
<p style="line-height:1.8">For more information click <a style="text-decoration:none;" href="https://static.igem.org/mediawiki/2015/1/1f/TU_Dresden_lzt18_first.pdf"><font color="#045FB4">here</font></a></p>
 
<figure align="center">
 
<figure align="center">
 
<a href="https://static.igem.org/mediawiki/2015/c/cd/TU_Dresden_lzt18_bacth_first_cut.jpg" title="View">
 
<a href="https://static.igem.org/mediawiki/2015/c/cd/TU_Dresden_lzt18_bacth_first_cut.jpg" title="View">

Revision as of 18:15, 14 September 2015


Plasmid Design

All plasmids designed in this section were used as parts for the various subprojects. These plasmids were initially designed to have kanomycin selection marker. These were synthesized from IDT. Below, the plasmid maps of the various parts characterized as for this project can be found.

Please refer to the Parts section for the final updated parts in iGEM Biobrick format.

Plasmid Maps

M13 gene 3 coding for infection protein P3

For more information click here

HER2 (Human Epidermal Growth Factor 2) cytoplasmic domain

For more information click here

ZHER2 - affibody with affinity to HER2

For more information click here

T25 - subunit of BACTH system

For more information click here

T18 - subunit of BACTH system

For more information click here

Leucine zipper able to bind to T25 BACTH subunit

For more information click here

Leucine zipper able to bind to T18 BACTH subunit

For more information click here