Overview
A signal filter that simplifies complex vibration is vital in information processing[1]. This summer, we aim to build one and explore its application in biocomputing and oral health care. Light inputs are achieved by our light-sensing protein and then further processed by a negative feedback circuit[2]. The criticality detector generates pulse output when the input’s vibration exceeds predefined threshold. For biocomputing, the detector is equipped with a binary system to store infor-mation in a “living register”. For oral health care, the detector is used to control the dosage of a powerful drug[3].
Overview
The detection of threshold is achieved with the help of a red-light-sensing part and a specially designed negative feedback circuit. A fusion protein called Cph8 senses red light and activates three OmpC promoters when red light intensity in the environment drops below a threshold. An output (GFP) keeps being generated until it is inhibited by the negative feedback circuit we built (see Fig.2). A relatively long feedback circuit was designed to generate pulse of good intensity and modularity.
Considering the system construction and work pattern, our criticality detection system can function as a pulse generator, a signal filter and so on. In our case, the module is proved to have potential application in biocomputer development and health care.
Our system can be used as a signal filter in the biocomputer development. Combining criticality detection system with pairs of integrase and excisionase, we set up a binary counting system composed of some two-state latch modules that switch between states “0” and “1”.
Working as a pulse generator in dental caries prevention, the system release a suitable amount of antimicrobial peptides by adjusting the length and intensity of the pulse and maintain the population of oral flora at a satisfactory level.
Considering the system construction and work pattern, our criticality detection system can function as a pulse generator, a signal filter and so on. In our case, the module is proved to have potential application in biocomputer development and health care.
Our system can be used as a signal filter in the biocomputer development. Combining criticality detection system with pairs of integrase and excisionase, we set up a binary counting system composed of some two-state latch modules that switch between states “0” and “1”.
Working as a pulse generator in dental caries prevention, the system release a suitable amount of antimicrobial peptides by adjusting the length and intensity of the pulse and maintain the population of oral flora at a satisfactory level.
