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In order to kill our bacteria which would imply the end of the game, we thought to use liposomes as toxin-mediated delivery. The idea is to create a bubble into the microfluidic chip containing the bacteria and the liposomes. As a result, when the bubble would touch the lasers, the liposomes would free up toxins that would kill the bacteria afterwards. | In order to kill our bacteria which would imply the end of the game, we thought to use liposomes as toxin-mediated delivery. The idea is to create a bubble into the microfluidic chip containing the bacteria and the liposomes. As a result, when the bubble would touch the lasers, the liposomes would free up toxins that would kill the bacteria afterwards. | ||
Revision as of 15:28, 15 September 2015
What is the Game Over?
Allowing the end of the game
The game over concerns the end of one round in the Bioconsole game.
Indeed, you can not play indefinitely with the Bioconsole, and as every funny game something can happen to the hero!
Here, the end of the game is the death of the bacteria.
As you know, the goal of the game is simple: to allow BACT'Man to survive as long as possible. Stop him from getting in contact with the virtual lasers that will appear faster and faster as the game goes on. If BACT'Man were to be touched, a bright flash will appear at the microscope and will activate the liposomes opening.
The toxins will eliminate our hero that will lose his fluorescence ... GAME OVER
Our game over is composed of two parts : Toxin-mediated delivery by photopolymerizable liposomes , clean-up of the microfluidic channel.
Photopolymerizable liposome
Toxin-mediated delivery
In order to kill our bacteria which would imply the end of the game, we thought to use liposomes as toxin-mediated delivery. The idea is to create a bubble into the microfluidic chip containing the bacteria and the liposomes. As a result, when the bubble would touch the lasers, the liposomes would free up toxins that would kill the bacteria afterwards.
For this to be possible, we wanted to use photopolymerizable liposomes. The latter composed of photopolymerizable phospholipid (DC8,9PC) would release entrapped toxins upon visible light treatment (The photopolymerization occurs at 514 nm).
Liposomes prepared from DC8,9PC alone form tube like structures that are not suitable for molecule entrapment, therefore, we thought to make a formulation containing a mixture of lipids (VIEW PROTOCOLE) .
In order to ensure a functional liposomes, we took as reference the article written by Yavlovich et al. (2011) entitled “A Novel class of Photo-triggerable liposomes containing DPPC:DC8,9PC as Vehicles for Delivery of Doxorubcin to Cells”.
BactMan - Game Over
Because of a time issue, we did not have enough time to synthesize the liposome and therefore test the first part of our game over.
According Yavlovich’s paper and if we follow carefully its liposome formation protocol, this system has a high probability to work efficiently.
Nevertheless, visible-light induced solute leakage from the liposome and depends on the spectral properties of entrapped solutes. As we did not test the liposome with the toxins, we cannot say if the leakage will be important or not. In any case, we do not really have to worry about it since we play upon red light.
PROJECT
BactMan's Adventures
BIOBRICKS
Our parts of DNA
MICROFLUIDICS
Our challenge in achieving the Bio-console