Difference between revisions of "Team:Goettingen/Notebook"

Line 23: Line 23:
 
     }
 
     }
  
−
     #menu1, #menu2, #menu3, #menu4, #menu5, #menu6, #menu7, #menu8, #menu9, #menu10, #menu11, #menu12, #menu13, #menu14, #menu15, #menu16, #menu17, #menu18, #menu19, #menu20, #menu21, #menu22, #menu23, #menu24{
+
     #menu1, #menu2, #menu3, #menu4, #menu5, #menu6, #menu7, #menu8, #menu9, #menu10, #menu11, #menu12, #menu13, #menu14, #menu15, #menu16, #menu17, #menu18, #menu19, #menu20, #menu21, #menu22, #menu23, #menu24, #menu25{
 
         display:none;
 
         display:none;
 
         width:90%;
 
         width:90%;
Line 1,236: Line 1,236:
 
     Dennis is happy to help with the organisation of the poster
 
     Dennis is happy to help with the organisation of the poster
 
</p>
 
</p>
 +
</div>
  
 
<a href="" onClick=" $('#menu18').slideToggle(300, function callback() {  }); return false;"><h2>18.08.15</h2></a>
 
<a href="" onClick=" $('#menu18').slideToggle(300, function callback() {  }); return false;"><h2>18.08.15</h2></a>
Line 1,264: Line 1,265:
 
</div>
 
</div>
  
 +
<a href="" onClick=" $('#menu20').slideToggle(300, function callback() {  }); return false;"><h2>24.08.15</h2></a>
 +
 +
<div id="menu20">
 +
<p>
 +
    <strong>1.) Labwork:</strong>
 +
</p>
 +
<p>
 +
    Each section of labwork needs to be reassigned in order to optimize our work
 +
</p>
 +
<p>
 +
    Dennis &amp; Julian will work on Gelfiltrations and Protein extractions. They may need some help with all the inductions.
 +
</p>
 +
<p>
 +
    Alaa will make sure all protocols are written for the Wiki in time for the Wiki Freeze. Every group needs to write up their protocols and results.
 +
</p>
 +
<p>
 +
    Avril will make Primers for BioBricks of Dockerins with and without Enzymes and ask the Aachen Team for some Enzymes (design Primers for these as well)
 +
</p>
 +
<p>
 +
    Stefi will work on Poster and Presentation
 +
</p>
 +
<p>
 +
    Enzyme Dockerin constructs and BioBricks need to be continued and brought to work. See if we can find an enzyme cascade that we could attach to the
 +
    Flexosome.
 +
</p>
 +
<p>
 +
    <strong>2.) Finances:</strong>
 +
</p>
 +
<p>
 +
    An overview over our current finances has to be made to know what we have for Tshirts and labcosts.
 +
</p>
 +
<p>
 +
    <strong>3.) Safety Form:</strong>
 +
</p>
 +
<p>
 +
    The safety form deadline is this week. Avril will work on it and double check it with Heiko.
 +
</p>
 +
</div>
 +
<a href="" onClick=" $('#menu21').slideToggle(300, function callback() {  }); return false;"><h2>27.08.15</h2></a>
 +
 +
<div id="menu21">
 +
<p>
 +
    &lt;p&gt;
 +
</p>
 +
<p>
 +
    <strong>1.) Labwork:</strong>
 +
</p>
 +
<p>
 +
    Enzymes: Phosphatase activity test in different Buffer is working
 +
</p>
 +
<p>
 +
    Cellulase: Primers have not come yet
 +
</p>
 +
<p>
 +
    Induction: Pho_CTHE and rfp_ACEL are in fat medium and the spare amount will be used with the FPLC purification.
 +
</p>
 +
<p>
 +
    Primers have been designed for most constructs
 +
</p>
 +
<p>
 +
    Verena, Udhaya and Sindhu will continue the work on Dockerins, Est_BCEl and cjblue for BioBrick creation.
 +
</p>
 +
<p>
 +
    <strong>2.) Wiki:</strong>
 +
</p>
 +
<p>
 +
    Alaa and Udhaya have been working on the Method Collection for the Wiki
 +
</p>
 +
<p>
 +
    Ask Alex to make it pretty and insert the Aix-Marseille Gold Medal for collaboration
 +
</p>
 +
<p>
 +
    <strong>3.) ETC:</strong>
 +
</p>
 +
<p>
 +
    Steffi is working on Poster and Presentation. She has designed Tshirts with Verena and both have looked for enzyme pathways within the biobricks.
 +
</p>
 +
 +
</div>
 +
<a href="" onClick=" $('#menu22').slideToggle(300, function callback() {  }); return false;"><h2>03.09.15</h2></a>
 +
 +
<div id="menu22">
 +
<p>
 +
<p>
 +
    <strong>1.) Labwork:</strong>
 +
</p>
 +
<p>
 +
    Protein purification of CTHE has been successful. Other constructs are not working. Not sure whether due to pBAD vector, therefore sequences need to be
 +
    checked.
 +
</p>
 +
<p>
 +
    Aachen Enzymes have arrived and the first BioBricks made (still need to be sequenced). BioBricks with colours amilCP, cjBlue and efforRed are being used as
 +
    backup.
 +
</p>
 +
<p>
 +
    Purification: Could the enzymes be deactivated by the purification?
 +
</p>
 +
<p>
 +
    Can we check scaffoldin interactions, even though enzymes may not function? So far it hasnt been possible to purify any of the enzymes or RFP and not to
 +
    show a 100% positive activity test.
 +
</p>
 +
<p>
 +
    - Restart expression tests
 +
</p>
 +
<p>
 +
    - start cellulase induction
 +
</p>
 +
<p>
 +
    - check RFP again with a control
 +
</p>
 +
<p>
 +
    - could it be possible to use the scaffoldin to purify the enzyme-dockerins?
 +
</p>
 +
<p>
 +
    - check for pBAD promoter error and ask Robert for other expression vectors.
 +
</p>
 +
<p>
 +
    - try and get some sort of interaction even if it is crude
 +
</p>
 +
<p>
 +
    - is there a BioBrick expression vector?
 +
</p>
 +
<p>
 +
    <strong>2.) Presentation:</strong>
 +
</p>
 +
<p>
 +
    First presentation meeting today
 +
</p>
 +
</div>
 +
<a href="" onClick=" $('#menu23').slideToggle(300, function callback() {  }); return false;"><h2>10.09.15</h2></a>
 +
 +
<div id="menu25">
 +
<p>
 +
<p>
 +
    <strong>1.) BioBricks:</strong>
 +
</p>
 +
<p>
 +
    Est-BCEL plasmid needs to be re-inoculated to get more plasmid. ACEL, BCEL and CCEL plasmids in the BioBrick vector look good and just need sequencing.
 +
</p>
 +
<p>
 +
    <strong>2.) Aachen:</strong>
 +
</p>
 +
<p>
 +
    HPS and PHI have been succesfully inserted into pBAD with sequencing being fine. Primers for expression in pET100 have arrived.
 +
</p>
 +
<p>
 +
    <strong>3.) Colours:</strong>
 +
</p>
 +
<p>
 +
    amilCP and eforRed have been sequenced but only amilCP is good, cjBlue is still in the BB vectore´
 +
</p>
 +
<p>
 +
    <strong>4.) Cellulase:</strong>
 +
</p>
 +
<p>
 +
    Restriction control in pBAD is good. Insertion into pET100 can be tried.
 +
</p>
 +
<p>
 +
    <strong>5.) pET100:</strong>
 +
</p>
 +
<p>
 +
    Esterase and Phosphatase have been inserted into pET100 with the dockerins. Need to wait if ligation and transformation has worked or if it needs to be
 +
    redone.
 +
</p>
 +
</p>
 +
</div>
  
 
</html>
 
</html>

Revision as of 17:02, 15 September 2015



Notebook

15.12.14

08.01.15

13.01.15

15.01.15

19.01.15

02.02.15

16.02.15

18.02.15

23.02.15

16.03.15

23.3.15

07.04.15

13.04.15

21.05.15

19.0615

25.06.15

24.07.15

18.08.15

24.08.15

27.08.15

03.09.15

10.09.15