The first component of our project focused on will be used to determine if the light produced by the lux box is sufficient to activate the light-activated transcription factor. This experiment requires a new component to the aforementioned genetic circuit in pDawn. The new component is initiated by a constitutive promoter (BBa_J23100) and followed by an RBS + lux box. Expression of the genes in the lux box is responsible for producing luminescence. The box contains the following genes in order: LuxC, D, A, B, E and G. LuxA and B are responsible for encoding two subunits of bacterial luciferase. The genes LuxC, D, and E code for the substrate for the light emitting reaction, tetradecanal. The function of the luxG gene is yet to be fully elucidated; however, inclusion of the gene is known to increase light output. The circuit is completed with a stop codon and a terminator sequence.

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