Difference between revisions of "Team:ZJU-China/Notebook"

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<p>Took photos and prepared for the banner~</p>
 
<p>Took photos and prepared for the banner~</p>
 
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<div class="tl-body">
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<p>Bacteria solution PCR: metK and OrfX. Both had positive clones. DNA sequencing: metK and OrfX.  T4 ligation and transformation: plu0840+pSB1C3, plu1537+pSB1C3, tcdA1+pSB1C3. Selected from single colonies: CDS-tcdA1.</p>
+
<p>Bacteria solution PCR: metK and OrfX. Both had positive clones. </p>
 +
<li><p class="p1">DNA sequencing: metK and OrfX.  </p></li>
 +
<li><p class="p1">T4 ligation and transformation: plu0840+pSB1C3, plu1537+pSB1C3, tcdA1+pSB1C3. </p></li>
 +
<li><p class="p1">Selected from single colonies: CDS-tcdA1. </p></li>
 
</div>
 
</div>
 
</div>
 
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<ul>
 
<ul>
 
<li><p class="p1">Selected from single colonies of metK and OrfX.</p></li>
 
<li><p class="p1">Selected from single colonies of metK and OrfX.</p></li>
−
<li><p class="p1">Submit the safety form. Selected from single colonies: CDS-plu0840. Double enzyme digestion and DNA gel extraction: plu1537, tcdA1, plu0840. Plasmid extration and double enzyme digestion: RFP. Protein extraction and SDS-PAGE: tcdA1, plu1537, plu0840. T4 ligation and transformation: plu0840+pSB1C3, plu1537+pSB1C3, tcdA1+pSB1C3. Bacteria solution PCR: CDS-plu0840.</p></li>
+
<li><p class="p1">Submit the safety form. </p></li>
 +
<li><p class="p1">Selected from single colonies: CDS-plu0840. </p></li>
 +
<li><p class="p1"> Double enzyme digestion and DNA gel extraction: plu1537, tcdA1, plu0840. </p></li>
 +
<li><p class="p1">Plasmid extration and double enzyme digestion: RFP.</p></li>
 +
<li><p class="p1">Protein extraction and SDS-PAGE: tcdA1, plu1537, plu0840.</p></li>
 +
<li><p class="p1">T4 ligation and transformation: plu0840+pSB1C3, plu1537+pSB1C3, tcdA1+pSB1C3. </p></li>
 +
<li><p class="p1">Bacteria solution PCR: CDS-plu0840.</p></li>
 
</ul>
 
</ul>
 
</div>
 
</div>
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
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<p id="e23">Seamless assembly: plasmid+OrfX, plasmid+metK. PCR: CDS-tcdA1. Double enzyme digestion: RFP+plasmid. Seamless assembly: CDS-plu0840. PCR and DNA gel extraction: CDS-tcdA1. Changed pSB1A3 to pSB1C3: plu0840, plu1537, tcdA1. Transformed them into E.coli DH5α, selected from single colonies and used single enzyme digestion to verify the got plasmids.</p>
+
<p id="e23">Seamless assembly: plasmid+OrfX, plasmid+metK. PCR: CDS-tcdA1.</p>
 +
<li><p class="p1">Double enzyme digestion: RFP+plasmid.</p></li>
 +
<li><p class="p1">Seamless assembly: CDS-plu0840.</p></li>
 +
<li><p class="p1">PCR and DNA gel extraction: CDS-tcdA1.</p></li>
 +
<li><p class="p1">Changed pSB1A3 to pSB1C3: plu0840, plu1537, tcdA1. </p></li>
 +
<li><p class="p1">Transformed them into E.coli DH5α, selected from single colonies and used single enzyme digestion to verify the got plasmids.</p></li>
 
</div>
 
</div>
 
</div>
 
</div>
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>Seamless assembly: plasmid+CDS-tcdA1, plasmid+CDS-plu0840. DNA gel extraction: tcdA1. PCR: device-plu0840.</p>
+
<p>Seamless assembly: plasmid+CDS-tcdA1, plasmid+CDS-plu0840.</p>
 +
<li><p class="p1">DNA gel extraction: tcdA1. PCR: device-plu0840.</p></li>
 
</div>
 
</div>
  
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<div class="tl-body">
 
<div class="tl-body">
 
<ul>
 
<ul>
−
<li><p class="p1">Bacteria solution PCR: metK and OrfX. Only negative clone. Extraction of plasmid mCherry and double enzyme digestion. DNA sequencing: ermE+plasmid, Frr+plasmid, plu1537+plasmid. Selected from single colonies of plu1537.</p></li>
+
<li><p class="p1">Bacteria solution PCR: metK and OrfX. Only negative clone. DNA sequencing: ermE+plasmid, Frr+plasmid, plu1537+plasmid.</p></li>
 +
<li><p class="p1">Extraction of plasmid mCherry and double enzyme digestion. </p></li>
 +
<li><p class="p1">Selected from single colonies of plu1537.</p></li>
 +
<br>
 
<li><p class="p1">Power cut. We played table tennis near our lab.</p></li>
 
<li><p class="p1">Power cut. We played table tennis near our lab.</p></li>
 
</ul>
 
</ul>
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<ul>
 
<ul>
 
<li><p class="p1">Selected from single colonies of metK and OrfX.</p></li>
 
<li><p class="p1">Selected from single colonies of metK and OrfX.</p></li>
−
<li><p class="p1">Transformed standard plu1537 plasmid into BL-21. Bacterium solution PCR: ermE and Frr.</p></li>
+
<li><p class="p1">Transformed standard plu1537 plasmid into BL-21.</p></li>
 +
<li><p class="p1">Bacterium solution PCR: ermE and Frr.</p></li>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2015/thumb/5/5c/8-23.jpg/450px-8-23.jpg" class="img-responsive">
 +
<br>
 
<li><p class="p1">We kept a Madagascar cockroach as our pet~</p></li>
 
<li><p class="p1">We kept a Madagascar cockroach as our pet~</p></li>
 
</ul>
 
</ul>
 
</div>
 
</div>
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<br>
 
−
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<br><br>
 
<br><br>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/06/8-23-3.jpg/800px-8-23-3.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/06/8-23-3.jpg/800px-8-23-3.jpg" class="img-responsive">
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<br><br>
 
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<img src="https://static.igem.org/mediawiki/2015/thumb/b/b0/8-23-4.jpg/800px-8-23-4.jpg" class="img-responsive">
 
 
</div>
 
</div>
 
</li>
 
</li>
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<ul>
 
<ul>
 
<li><p class="p1">DNA sequencing. Seamless assembly: plasmid+1537, plasmid+tcdA1-L+ tcdA1-M+ tcdA1-R, plasmid+ermE, plasmid+Frr. </p></li>
 
<li><p class="p1">DNA sequencing. Seamless assembly: plasmid+1537, plasmid+tcdA1-L+ tcdA1-M+ tcdA1-R, plasmid+ermE, plasmid+Frr. </p></li>
−
<li><p class="p1">Successfully booked the HI-Boston Hostel and paid the full payment~ Seamless assembly: plasmid+metK, plasmid+OrfX. </p></li>
+
<li><p class="p1">Seamless assembly: plasmid+metK, plasmid+OrfX. </p></li>
 +
<li><p class="p1">Successfully booked the HI-Boston Hostel and paid the full payment~</p></li>
 
</ul>
 
</ul>
 
</div>
 
</div>
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>38th Group meeting. Introduced a software named Mou which could enhance the efficiency of writing wiki. Discussed the business planning of our product and the photos on the wiki. </p>
+
<p>Introduced a software named Mou which could enhance the efficiency of writing wiki. Discussed the business planning of our product and the photos on the wiki. </p>
 
</div>
 
</div>
 
</div>
 
</div>
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<div class="tl-body">
 
<div class="tl-body">
 
<ul>
 
<ul>
−
<li><p class="p1">PCR: ermE, Frr, metK and OrfX. Clean up and DNA gel extraction. </p></li>
 
 
<li><p class="p1">Finished the background of avermectin and toxic protein. Sent bacterium sample to BNU and we would continue to help them.</p></li>
 
<li><p class="p1">Finished the background of avermectin and toxic protein. Sent bacterium sample to BNU and we would continue to help them.</p></li>
 +
<li><p class="p1">PCR: ermE, Frr, metK and OrfX. Clean up and DNA gel extraction. </p></li>
 
</ul>
 
</ul>
 
</div>
 
</div>
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<div class="tl-body">
 
<div class="tl-body">
 
<ul>
 
<ul>
−
<li><p class="p1">PCR: CDS tcdB1, CDS plu1537 and mCherry. DNA gel extraction: tcdA1-L and plu0840L. Seamless assembly: plasmid+pBad+plu1537.</p></li>
+
<li><p class="p1">PCR: CDS tcdB1, CDS plu1537 and mCherry.</p></li>
 +
<li><p class="p1">DNA gel extraction: tcdA1-L and plu0840L.</p></li>
 +
<li><p class="p1">Seamless assembly: plasmid+pBad+plu1537.</p></li>
 
<li><p class="p1">Detected the relationship between distance of food source and food consumption.</p></li>
 
<li><p class="p1">Detected the relationship between distance of food source and food consumption.</p></li>
 
<li><p class="p1">Designed the primers for Frr, metK, OrfX and ermE again because the need for standardization.</p></li>
 
<li><p class="p1">Designed the primers for Frr, metK, OrfX and ermE again because the need for standardization.</p></li>
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>37th Group meeting. Built the framework for wiki. Discussed the Syn-bio talks on next Saturday.</p>
+
<p>Built the framework for wiki. Discussed the Syn-bio talks on next Saturday.</p>
 
</div>
 
</div>
  
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<div class="tl-body">
 
<div class="tl-body">
 
<ul>
 
<ul>
−
<li><p class="p1">PCR: pBad, tcdA1-L, tcdB1, plu0840L and plu1537. Purchased E.coli BL21.</p></li>
+
<li><p class="p1">PCR: pBad, tcdA1-L, tcdB1, plu0840L and plu1537.</p></li>
 +
<li><p class="p1">Purchased E.coli BL21.</p></li>
 
<li><p class="p1">The experiment concerning Streptomyces avermitilis finished 80%.</p></li>
 
<li><p class="p1">The experiment concerning Streptomyces avermitilis finished 80%.</p></li>
 
<li><p class="p1">Lovely poppy~ </p></li>
 
<li><p class="p1">Lovely poppy~ </p></li>
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<div class="tl-body">
 
<div class="tl-body">
 
<ul>
 
<ul>
−
<li><p class="p1">PCR and clean up. Concluded the successful PCR parameters of different gene clusters.</p></li>
 
 
<li><p class="p1">Test the Avermectin detection kit with pure Ivermectin.</p></li>
 
<li><p class="p1">Test the Avermectin detection kit with pure Ivermectin.</p></li>
 
<li><p class="p1">Designed the primers for plu0840, plu1537, tcdA1 and tcdB1 again bucause of the need for standardization.</p></li>
 
<li><p class="p1">Designed the primers for plu0840, plu1537, tcdA1 and tcdB1 again bucause of the need for standardization.</p></li>
 +
<li><p class="p1">PCR and clean up. Concluded the successful PCR parameters of different gene clusters.</p></li>
 
</ul>
 
</ul>
 
</div>
 
</div>
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<li><p class="p1">Overlap: pBad and tcdA1-L, pBad and tcd-B1, pBad and plu1537. Extraction of plasmid mCherry and enzyme digestion.</p></li>
 
<li><p class="p1">Overlap: pBad and tcdA1-L, pBad and tcd-B1, pBad and plu1537. Extraction of plasmid mCherry and enzyme digestion.</p></li>
 
<li><p class="p1">Completed wiki page for 2nd model. Drew the 3D ball-and-stick models for CNC with Solidworks. Completed the device for killing termites. </p></li>
 
<li><p class="p1">Completed wiki page for 2nd model. Drew the 3D ball-and-stick models for CNC with Solidworks. Completed the device for killing termites. </p></li>
−
<li><p class="p1">Yummy hotpot~</p></li>
 
 
<li><p class="p1">Completed the SCM program for HAZU and they affirmed our help.</p></li>
 
<li><p class="p1">Completed the SCM program for HAZU and they affirmed our help.</p></li>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2015/0/0e/8-13.jpg" class="img-responsive">
 +
<li><p class="p1">Yummy hotpot~</p></li>
 
</ul>
 
</ul>
 
</div>
 
</div>
 
<br>
 
<br>
−
<img src="https://static.igem.org/mediawiki/2015/0/0e/8-13.jpg" class="img-responsive">
 
−
<br><br>
 
 
<img src="https://static.igem.org/mediawiki/2015/thumb/3/3b/8-13-2.jpg/450px-8-13-2.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/3/3b/8-13-2.jpg/450px-8-13-2.jpg" class="img-responsive">
 
</div>
 
</div>
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<li><p class="p1">PCR: pBad, tcdA1-L, tcdA1-M, tcdA1-R, tcdB1, 0840L, 0840R, 1537, GFP.</p></li>
 
<li><p class="p1">PCR: pBad, tcdA1-L, tcdA1-M, tcdA1-R, tcdB1, 0840L, 0840R, 1537, GFP.</p></li>
 
<li><p class="p1">PCR: ligated product. Optimized the PCR program. Cleaned up. PCR again.  </p></li>
 
<li><p class="p1">PCR: ligated product. Optimized the PCR program. Cleaned up. PCR again.  </p></li>
−
<li><p class="p1">SEM scanned our samples to verify whether Streptomyces avermitilis were successfully embedded with CNC.</p></li>
 
 
<li><p class="p1">Fed the termites with Photorhabdus luminescens TT01 bacterium cells.</p></li>
 
<li><p class="p1">Fed the termites with Photorhabdus luminescens TT01 bacterium cells.</p></li>
 +
<li><p class="p1">SEM scanned our samples to verify whether Streptomyces avermitilis were successfully embedded with CNC.</p></li>
 
</ul>
 
</ul>
 
</div>
 
</div>
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<li><p class="p1">PCR: pBad, tcdA1-L and GFP.</p></li>
 
<li><p class="p1">PCR: pBad, tcdA1-L and GFP.</p></li>
 
<li><p class="p1">Used Bgl2 and EcoR1 digested inserted fragment and cleaned up the product. Ligating fragment into plasmid with T4 ligase.</p></li>
 
<li><p class="p1">Used Bgl2 and EcoR1 digested inserted fragment and cleaned up the product. Ligating fragment into plasmid with T4 ligase.</p></li>
−
<li><p class="p1">Specified the standardization requirements and laid the foundation for primer designing.</p></li>
 
 
<li><p class="p1">Mixed blue-stained termites with red-stained termites to verify trophallaxis.</p></li>
 
<li><p class="p1">Mixed blue-stained termites with red-stained termites to verify trophallaxis.</p></li>
 +
<li><p class="p1">Specified the standardization requirements and laid the foundation for primer designing.</p></li>
 
</ul>
 
</ul>
 
</div>
 
</div>
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>35th Group meeting. Planned to hold a seminar for freshmen. Tried to design a device for attracting and killing termites.</p>
+
<p>Planned to hold a seminar for freshmen. Tried to design a device for attracting and killing termites.</p>
 
</div>
 
</div>
 
</div>
 
</div>
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<ul>
 
<ul>
 
<li><p class="p1">PCR: tcdA1-M, tcdA1-R, tcdB1, plu0840L, plu0840R and plu1537.</p></li>
 
<li><p class="p1">PCR: tcdA1-M, tcdA1-R, tcdB1, plu0840L, plu0840R and plu1537.</p></li>
−
<li><p class="p1" id = "e21">Completed our framework and philosophy of safety.</p></li>
 
 
<li><p class="p1">Extracted the plasmid containing RFP from DH5α. PCR: RFP and confirmed by gel electrophoresis.</p></li>
 
<li><p class="p1">Extracted the plasmid containing RFP from DH5α. PCR: RFP and confirmed by gel electrophoresis.</p></li>
 +
<li><p class="p1" id = "e21">Completed our framework and philosophy of safety.</p></li>
 
</ul>
 
</ul>
 
</div>
 
</div>
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</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/3/38/8-8-2.png/800px-8-8-2.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/3/38/8-8-2.png/800px-8-8-2.png" class="img-responsive">
−
</div>
+
<br>
−
</li>
+
<br>
−
 
+
−
 
+
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<li class="entry timeline-inverted">
+
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<div class="timeline-panel">
+
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<div class="tl-heading">
+
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<p><small class="text-muted">8.8.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p id="e20">Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).</p>
+
−
</div>
+
 
<img src="https://static.igem.org/mediawiki/2015/thumb/b/bb/8-8-3.jpg/800px-8-8-3.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/b/bb/8-8-3.jpg/800px-8-8-3.jpg" class="img-responsive">
 +
<br><br>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/8/8f/8-8.jpeg/800px-8-8.jpeg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/8/8f/8-8.jpeg/800px-8-8.jpeg" class="img-responsive">
 
</div>
 
</div>
 
</li>
 
</li>
 +
  
  
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>One step cloning protocol finished. Designed the questionaire about termites.</p>
+
<p>One step cloning protocol finished.</p>
 +
<li><p class="p1">Designed the questionaire about termites.</p></li>
 
</div>
 
</div>
 
</div>
 
</div>
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p id="e19">34th Group meeting. Communicated with 2 members of SJTU in our lab. Began to write wiki. Attached more importance to safety.</p>
+
<p id="e19">Communicated with 2 members of SJTU in our lab. Began to write wiki. Attached more importance to safety.</p>
 
</div>
 
</div>
 
</div>
 
</div>
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<div class="tl-body">
 
<div class="tl-body">
 
<ul>
 
<ul>
−
<li><p class="p1">Drawing plasmid circuits in snapgene.(toxic protein)</p></li>
 
 
<li><p class="p1">Stained 50 termites in blue and 50 in red for trophallaxis experiments.</p></li>
 
<li><p class="p1">Stained 50 termites in blue and 50 in red for trophallaxis experiments.</p></li>
 +
<li><p class="p1">Drawing plasmid circuits in snapgene.(toxic protein)</p></li>
 
</ul>
 
</ul>
 
</div>
 
</div>
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<div class="tl-body">
 
<div class="tl-body">
 
<ul>
 
<ul>
 +
<li><p class="p1">Decided to give up confocol because we couldn't find the specific dye for CNC.</p></li>
 
<li><p class="p1">Discussed the circuit construction and experiments planning of toxic protein.</p></li>
 
<li><p class="p1">Discussed the circuit construction and experiments planning of toxic protein.</p></li>
−
<li><p class="p1">Decided to give up confocol because we couldn't find the specific dye for CNC.</p></li>
 
 
</ul>
 
</ul>
 
</div>
 
</div>
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</div>
 
</div>
 
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<img src="https://static.igem.org/mediawiki/2015/thumb/b/bc/7-20.jpg/800px-7-20.jpg" class="img-responsive">
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<br><br>
 +
<img src="https://static.igem.org/mediawiki/2015/thumb/3/3b/7-19.jpg/800px-7-19.jpg" class="img-responsive">
 
</div>
 
</div>
 
</li>
 
</li>
  
  
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<li class="entry timeline-inverted">
 
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<div class="timeline-panel">
 
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<div class="tl-heading">
 
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<p><small class="text-muted">7.19.2015-7.24.2015</small></p>
 
−
</div>
 
−
<div class="tl-body" id = "e16">
 
−
<p>Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST. </p>
 
−
</div>
 
−
<img src="https://static.igem.org/mediawiki/2015/thumb/3/3b/7-19.jpg/800px-7-19.jpg" class="img-responsive">
 
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<img src="https://static.igem.org/mediawiki/2015/thumb/8/8a/6-17-3.png/798px-6-17-3.png" class="img-responsive">
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<br><br>
 +
<img src="https://static.igem.org/mediawiki/2015/thumb/7/74/6-17-2.jpg/590px-6-17-2.jpg" class="img-responsive">
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 +
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</li>
 
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−
<li class="entry timeline-inverted">
+
 
−
<div class="timeline-panel">
+
−
<div class="tl-heading">
+
−
<p><small class="text-muted">6.17.2015</small></p>
+
−
</div>
+
−
<div class="tl-body">
+
−
<p>TEM and SEM scanned our samples.</p>
+
−
</div>
+
−
<img src="https://static.igem.org/mediawiki/2015/thumb/7/74/6-17-2.jpg/590px-6-17-2.jpg" class="img-responsive">
+
−
<img src="https://static.igem.org/mediawiki/2015/a/a4/6-17.jpg" class="img-responsive">
+
  
 
</div>
 
</div>
Line 996: Line 994:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>27th Group meeting. Rearranged all the events with four quadrant method. Checked everything we have had. </p>
+
<p>Rearranged all the events with four quadrant method. Checked everything we have had. </p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/7/70/6-14.jpg/800px-6-14.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/7/70/6-14.jpg/800px-6-14.jpg" class="img-responsive">
Line 1,011: Line 1,009:
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/3/3d/6-14-2.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/3/3d/6-14-2.jpg" class="img-responsive">
 +
<br>
 +
<br>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/b/ba/6-14-3.png/800px-6-14-3.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/b/ba/6-14-3.png/800px-6-14-3.png" class="img-responsive">
  
Line 1,083: Line 1,083:
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/8/82/6-8-2.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/8/82/6-8-2.png" class="img-responsive">
 +
<br><br>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/2/27/6-8-3.png/800px-6-8-3.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/2/27/6-8-3.png/800px-6-8-3.png" class="img-responsive">
 +
<br><br>
 
<img src="https://static.igem.org/mediawiki/2015/1/1b/6-8.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/1/1b/6-8.png" class="img-responsive">
  
Line 1,118: Line 1,120:
 
<div class="tl-body">
 
<div class="tl-body">
 
<p id="e13">Frozen the spores of Streptomyces avermitilis.</p>
 
<p id="e13">Frozen the spores of Streptomyces avermitilis.</p>
 +
<li><p class="p1">Judge Haoqian Zhang from Peking University came to our lab. Frozen the spores of Streptomyces avermitilis.</p></li>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/03/6-6.jpeg/800px-6-6.jpeg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/03/6-6.jpeg/800px-6-6.jpeg" class="img-responsive">
Line 1,148: Line 1,151:
 
<li><p class="p1">Discussed the characterization of E.coli embedded in CNC, including TEM, SEM, confocal, FTIR and flow cytometry.</p></li>
 
<li><p class="p1">Discussed the characterization of E.coli embedded in CNC, including TEM, SEM, confocal, FTIR and flow cytometry.</p></li>
 
<li><p class="p1" id ="e12">Our proposal had been accepted and our team would be awarded financial support from SYNENERGENE!  Cheers~</p></li>
 
<li><p class="p1" id ="e12">Our proposal had been accepted and our team would be awarded financial support from SYNENERGENE!  Cheers~</p></li>
 +
<br>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2015/thumb/8/8d/6-3.png/800px-6-3.png" class="img-responsive">
 +
 
<li><p class="p1">Brainstormed how to do human practice and use the €5000. We came up with the  Synbio-cards and Synbio-workshops.</p></li>
 
<li><p class="p1">Brainstormed how to do human practice and use the €5000. We came up with the  Synbio-cards and Synbio-workshops.</p></li>
 
</ul>
 
</ul>
 
</div>
 
</div>
−
<img src="https://static.igem.org/mediawiki/2015/thumb/8/8d/6-3.png/800px-6-3.png" class="img-responsive">
 
−
 
 
</div>
 
</div>
 
</li>
 
</li>
Line 1,162: Line 1,167:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>Began to book the air tickets. Booked the first primers of toxic protein for one step cloning. Found the sequence of the gene we need in the Streptomyces avermitilis.</p>
+
<p>Began to book the air tickets.</p>
 +
<li><p class="p1">Booked the first primers of toxic protein for one step cloning.</p></li>
 +
<li><p class="p1">Found the sequence of the gene we need in the Streptomyces avermitilis.</p></li>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,190: Line 1,197:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>23rd Group meeting. Discussed the half-done modeling.</p>
+
<p>Discussed the half-done modeling.</p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,214: Line 1,221:
 
<div class="tl-body">Taught high school students to do experiment to extract DNA from epithelial cell of mouth.
 
<div class="tl-body">Taught high school students to do experiment to extract DNA from epithelial cell of mouth.
 
</div>
 
</div>
−
<img src="https://static.igem.org/mediawiki/2015/thumb/b/b7/5-31-3.jpg/800px-5-31-3.jpg" class="img-responsive">
+
<img src="https://static.igem.org/mediawiki/2015/thumb/7/78/5-31.jpg/800px-5-31.jpg" class="img-responsive">
 
<br><br>
 
<br><br>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/f/f5/5-31-2.jpg/800px-5-31-2.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/f/f5/5-31-2.jpg/800px-5-31-2.jpg" class="img-responsive">
 
<br><br>
 
<br><br>
−
<img src="https://static.igem.org/mediawiki/2015/thumb/7/78/5-31.jpg/800px-5-31.jpg" class="img-responsive">
+
<img src="https://static.igem.org/mediawiki/2015/thumb/b/b7/5-31-3.jpg/800px-5-31-3.jpg" class="img-responsive">
 
</div>
 
</div>
 
</li>
 
</li>
Line 1,231: Line 1,238:
 
<li><p class="p1">Repeated the cellulase activity detection experiment.  
 
<li><p class="p1">Repeated the cellulase activity detection experiment.  
 
Meanwhile we measured the lysozyme activity in different parts of termites guts.</p></li>
 
Meanwhile we measured the lysozyme activity in different parts of termites guts.</p></li>
−
<li><p class="p1">22nd Group meetig. Specified the medal requirement. Began to prepare for the  NCTU meetup.</p></li>
+
<li><p class="p1">22nd Group meeting. Specified the medal requirement. Began to prepare for the  NCTU meetup.</p></li>
 
<li><p class="p1">SEM scanned our samples. </p></li>
 
<li><p class="p1">SEM scanned our samples. </p></li>
 
</ul>
 
</ul>
Line 1,255: Line 1,262:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p id ="e11">Got the solid CNC with lyophilization. Built the framework of the first web page.</p>
+
<p id ="e11">Built the framework of the first web page.</p>
 +
<li><p class="p1">Got the solid CNC with lyophilization. </p></li>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/0b/5-26.png/800px-5-26.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/0b/5-26.png/800px-5-26.png" class="img-responsive">
Line 1,279: Line 1,287:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>21st Group meeting. Began to prepare for the presentation. </p>
+
<p> Began to prepare for the presentation. </p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,312: Line 1,320:
 
<p><small class="text-muted">5.21.2015</small></p>
 
<p><small class="text-muted">5.21.2015</small></p>
 
</div>
 
</div>
−
<div class="tl-body">20th Group meeting. Discussed the medal requirements and collaboration among groups.
+
<div class="tl-body">Discussed the medal requirements and collaboration among groups.
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,337: Line 1,345:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>Detailed the medal requirements depending on our project. Finished the protocol about Streptomyces avermitilis.</p>
+
<p>Detailed the medal requirements depending on our project.</p>
 +
<li><p class="p1">Finished the protocol about Streptomyces avermitilis.</p></li>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/8/89/5-19-2.png/800px-5-19-2.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/8/89/5-19-2.png/800px-5-19-2.png" class="img-responsive">
Line 1,364: Line 1,373:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>18th Group meeting. Emphasized the lab safety. </p>
+
<p>Emphasized the lab safety. </p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,401: Line 1,410:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>17th Group meeting. Discussed the results of the TEM. Tried to contact with a judge from PKU.</p>
+
<p>Discussed the results of the TEM. Tried to contact with a judge from PKU.</p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,420: Line 1,429:
 
<br>
 
<br>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/f/f4/5-14-3.jpg/800px-5-14-3.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/f/f4/5-14-3.jpg/800px-5-14-3.jpg" class="img-responsive">
−
<img src="https://static.igem.org/mediawiki/2015/thumb/4/48/5-14.jpg/590px-5-14.jpg" class="img-responsive">
 
 
</div>
 
</div>
 
</li>
 
</li>
Line 1,432: Line 1,440:
 
<ul>
 
<ul>
 
<li><p class="p1">TEM scanned our samples: using CNC to pack the Bacillus subtilis.</p></li>
 
<li><p class="p1">TEM scanned our samples: using CNC to pack the Bacillus subtilis.</p></li>
−
<li><p class="p1">Completed the cover and comic of our proposal which would be handed in to SYNENERGENE.</p></li>
+
 
−
<li><p class="p1">Contacted with Hangzhou Termites control Institute.</p></li>
+
 
</ul>
 
</ul>
 
</div>
 
</div>
 
<br>
 
<br>
 +
<img src="https://static.igem.org/mediawiki/2015/thumb/4/48/5-14.jpg/590px-5-14.jpg" class="img-responsive">
 +
<br><br>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/06/5-14-2.jpg/590px-5-14-2.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/06/5-14-2.jpg/590px-5-14-2.jpg" class="img-responsive">
 
</div>
 
</div>
Line 1,457: Line 1,466:
 
</ul>
 
</ul>
 
</div>
 
</div>
 +
<br>
 +
<br>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/c/c3/5-13.JPG/800px-5-13.JPG" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/c/c3/5-13.JPG/800px-5-13.JPG" class="img-responsive">
 
</div>
 
</div>
Line 1,480: Line 1,491:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>16th Group meeting. Communication between dry group and 3 wet groups. Arranged time for experiments.</p>
+
<p> Communication between dry group and 3 wet groups. Arranged time for experiments.</p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,492: Line 1,503:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>15th Group meeting. Further discussed the proposal and allocated the tasks. Continued to consult professors about several species of bacterium.</p>
+
<p> Further discussed the proposal and allocated the tasks. Continued to consult professors about several species of bacterium.</p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,555: Line 1,566:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>14th Group meeting. 10 members would go to Taiwan for NCTU meetup. Results of consulting professors specialized in termites and Bacillus subtilis.</p>
+
<p>10 members would go to Taiwan for NCTU meetup.</p>
 +
<li><p class="p1">Results of consulting professors specialized in termites and Bacillus subtilis.</p></li>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/a/ad/4-29.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/a/ad/4-29.png" class="img-responsive">
Line 1,594: Line 1,606:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>13th Group meeting. Discussed something important: the proposal for the 5000€ additional sponsership of SYNENERGENE and visa. A new mamber joined our team. Specified the problem remaining to be solved of each group.</p>
+
<p> Discussed something important: the proposal for the 5000€ additional sponsership of SYNENERGENE and visa. A new mamber joined our team. Specified the problem remaining to be solved of each group.</p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,617: Line 1,629:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>12th Group meeting. Debugged the previous package plan and decided to pack bacterium with CNC. Planned to buy a nest of termites from Guangdong Province. Discussed several demensions of modeling in this project.</p>
+
<p>Debugged the previous package plan and decided to pack bacterium with CNC. Planned to buy a nest of termites from Guangdong Province. Discussed several demensions of modeling in this project.</p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/a/a0/4-23-2.jpg/600px-4-23-2.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/a/a0/4-23-2.jpg/600px-4-23-2.jpg" class="img-responsive">
 +
<br>
 +
<br>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/a/a6/4-23.jpg/450px-4-23.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/a/a6/4-23.jpg/450px-4-23.jpg" class="img-responsive">
  
Line 1,645: Line 1,659:
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/8/81/4-21.png/450px-4-21.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/8/81/4-21.png/450px-4-21.png" class="img-responsive">
−
 
 
</div>
 
</div>
 
</li>
 
</li>
Line 1,655: Line 1,668:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>Communication with our instructor. Searched for information about cellulose binding domain(CBDs) and overexpression of ivermectin and avermectin.</p>
+
<p>Communication with our instructor. Searched for information about cellulose binding domain (CBDs) and overexpression of ivermectin and avermectin.</p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/1/18/4-20.png/553px-4-20.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/1/18/4-20.png/553px-4-20.png" class="img-responsive">
Line 1,669: Line 1,682:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p id = "e4">11th Group meeting. Decided our project: termite terminator. Allocated all members into 4 groups. Each group was responsible for a part of our project. </p>
+
<p id = "e4">Decided our project: termite terminator. Allocated all members into 4 groups. Each group was responsible for a part of our project. </p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/c/c0/4-19.jpg/800px-4-19.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/c/c0/4-19.jpg/800px-4-19.jpg" class="img-responsive">
Line 1,683: Line 1,696:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>10th Group meeting. Decided to participate in NCTU meetup in summer holiday and apply for the 5000€ additional sponsership of SYNENERGENE. Discussed 3 existing ideas.</p>
+
<p>Decided to participate in NCTU meetup in summer holiday and apply for the 5000€ additional sponsership of SYNENERGENE. Discussed 3 existing ideas.</p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,709: Line 1,722:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>9th Group meeting. Kept 3 ideas and the final idea would derive from them. Detailed these 3 ideas from all the aspects.</p>
+
<p>Kept 3 ideas and the final idea would derive from them. Detailed these 3 ideas from all the aspects.</p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/3/34/4-12.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/3/34/4-12.jpg" class="img-responsive">
Line 1,724: Line 1,737:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>8th Group meeting. Continued to kill unappropriate ideas. Recalled the aim of participating iGEM and reached a consensus.</p>
+
<p>Continued to kill unappropriate ideas. Recalled the aim of participating iGEM and reached a consensus.</p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,736: Line 1,749:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>7th Group meeting. Began to kill some undoable ideas and further dug the existing ideas, such as biological enigma machine and killing termites.</p>
+
<p>Began to kill some undoable ideas and further dug the existing ideas, such as biological enigma machine and killing termites.</p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/1/10/4-6-2.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/1/10/4-6-2.png" class="img-responsive">
 +
<br>
 +
<br>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/c/cf/4-6.png/800px-4-6.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/c/cf/4-6.png/800px-4-6.png" class="img-responsive">
 
</div>
 
</div>
Line 1,751: Line 1,766:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>6th Group meeting. Developed several new ideas, including bio-SCM, biological enigma machine and slow release of certain chemical. Talked about their feasibility.</p>
+
<p>Developed several new ideas, including bio-SCM, biological enigma machine and slow release of certain chemical. Talked about their feasibility.</p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/5/5f/4-2-2.jpg/800px-4-2-2.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/5/5f/4-2-2.jpg/800px-4-2-2.jpg" class="img-responsive">
 +
<br><br>
 
<img src="https://static.igem.org/mediawiki/2015/f/f3/4-2.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/f/f3/4-2.png" class="img-responsive">
 
</div>
 
</div>
Line 1,771: Line 1,787:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>5th Group meeting. Dug previous ideas and considered their safety and feasibility.</p>
+
<p>Dug previous ideas and considered their safety and feasibility.</p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,783: Line 1,799:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>4th Group meeting. Continued to share and improve our ideas, such as bio-lens, detection of schistosomiasis and killing termites.</p>
+
<p>Continued to share and improve our ideas, such as bio-lens, detection of schistosomiasis and killing termites.</p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,795: Line 1,811:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p id = e3>Team application completed.</p>
+
<p id = e3>Team registration completed.</p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/04/3-25.png/431px-3-25.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/04/3-25.png/431px-3-25.png" class="img-responsive">
Line 1,810: Line 1,826:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>3rd Group meeting. Discussed some ideas about modeling. Shared new ideas.</p>
+
<p>Discussed some ideas about modeling. Shared new ideas.</p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,835: Line 1,851:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>2nd Group meeting. Built up several rules and principles. Tried to make the most of our BBS.</p>
+
<p>Built up several rules and principles. Tried to make the most of our BBS.</p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,874: Line 1,890:
 
<p id="Winter">Concluded the winter project.</p>
 
<p id="Winter">Concluded the winter project.</p>
 
</div>
 
</div>
−
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Revision as of 05:11, 16 September 2015

Notebook

We listed 23 pieces of Breaking News. Here are the links:

Croll down to see more.

  • September

  • 9.5.2015

    Finished the overview of entire project.

  • 9.4.2015

    • Bacteria solution PCR: OrfX. One positive clone of 20 samples.

    • DNA sequencing: OrfX.

  • 9.2.2015

    Dynamic Light Scattering(DLS) experiment to measure the particle size of embedded E.coli.

  • 9.1.2015

    The sequence of OrfX was not correct because of the wrong template, thus we had to do the related experiments again.

  • August

  • 8.31.2015

    Took photos and prepared for the banner~



  • 8.30.2015

    The sequence of metK and tcdA1 were confirmed, which marked the success in standardization of 2 parts!!

  • 8.29.2015

    Bacteria solution PCR: metK and OrfX. Both had positive clones.

  • DNA sequencing: metK and OrfX.

  • T4 ligation and transformation: plu0840+pSB1C3, plu1537+pSB1C3, tcdA1+pSB1C3.

  • Selected from single colonies: CDS-tcdA1.

  • 8.28.2015

    • Selected from single colonies of metK and OrfX.

    • Submit the safety form.

    • Selected from single colonies: CDS-plu0840.

    • Double enzyme digestion and DNA gel extraction: plu1537, tcdA1, plu0840.

    • Plasmid extration and double enzyme digestion: RFP.

    • Protein extraction and SDS-PAGE: tcdA1, plu1537, plu0840.

    • T4 ligation and transformation: plu0840+pSB1C3, plu1537+pSB1C3, tcdA1+pSB1C3.

    • Bacteria solution PCR: CDS-plu0840.

  • 8.27.2015

    Seamless assembly: plasmid+OrfX, plasmid+metK. PCR: CDS-tcdA1.

  • Double enzyme digestion: RFP+plasmid.

  • Seamless assembly: CDS-plu0840.

  • PCR and DNA gel extraction: CDS-tcdA1.

  • Changed pSB1A3 to pSB1C3: plu0840, plu1537, tcdA1.

  • Transformed them into E.coli DH5α, selected from single colonies and used single enzyme digestion to verify the got plasmids.

  • 8.26.2015

    Seamless assembly: plasmid+CDS-tcdA1, plasmid+CDS-plu0840.

  • DNA gel extraction: tcdA1. PCR: device-plu0840.