Choosing a non-pathogenic chassis

Bacterial strain: Escherichia coli K12

Escherichia coli K-12 are not a threat to human, animal (chickens, pigs, and calves), plants or the environment, according to the 1997 Final Risk Assessment of Environmental Protection Agency (EPA). “Any concerns in terms of health considerations are mitigated by its poor ability to disseminate, colonize the colon and establish infections in a murine model” (Smith et al., 2010). In addition, the chance of the insertion gene mutating the bacterial strain to be hazardous is minimal as the inserting genetic materials have to meet the EPA’s criteria (limited in size, well characterized, free of certain nucleotide sequences, and poor mobility).

Choosing parts that will not harm humans/ animals/ plants