Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w10"
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===pdark-GFP electro-transformation=== | ===pdark-GFP electro-transformation=== | ||
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| + | =====Sep. 8===== | ||
We picked 1 colony (only 1 colony appeared) from the pdark-GFP plate and spread them on another BG11 plate in line to do solid segregation | We picked 1 colony (only 1 colony appeared) from the pdark-GFP plate and spread them on another BG11 plate in line to do solid segregation | ||
Revision as of 08:54, 16 September 2015
Contents
Transformation
pdark natural transformation
in the evening of 11th, Sep
6th liquid segregation of pdark-HR
pcpcG2 electro-transformation
in the evening of 11th Sep
5th liquid segregation of pcpcG2-HR
first desalination test of pcpcG2-HR
Sep. 7
Inoculated 15 wildtype & 15 pcpcG2-HR (use pcpcG2-HR from colony 2) at 10pm.
Sep. 9
Induced with green light at 10pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each).
Sep. 10
Cultivated with red light at 10am, then took the second 6 samples,
Took the third 6 samples at 2pm.
Took the fourth 6 samples at 10pm.
Sep. 11
Took the fifth 6 samples at 10am.
pdark-GFP electro-transformation
Sep. 8
We picked 1 colony (only 1 colony appeared) from the pdark-GFP plate and spread them on another BG11 plate in line to do solid segregation