Difference between revisions of "Team:TU Darmstadt/Project/Bio/Monomeres/Xylitol"
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| + | <h2 align="center">Biotechnological production of Xylitol in <em>Escherichia coli</em></h2> | ||
| + | <p> </p> | ||
| + | <p>In the same way itaconic acid can be used to do cross-links due to its various reactive groups, the sugar substitute Xylitol can be used in related applications. Xylitol, as known as Xylit, occurs in slight amounts in several vegetables and in the rind of trees. Because of the dry mass of just 1%, conventional extraction out of these plants has no high economic value. At this point we, the iGEM-Team TU Darmstadt 2015, aim to add an alternative way of producing xylitol using biological fermentation containing specifically genetically modified <em>Escherichia coli</em>.</p> | ||
| + | <p>As a sugar alcohol xylitol is a relevant substance for pharmaceuticals, oral and personal care product and as precursor for chemical synthesis. Because of these applications a microbial based industrial scale production gets more and more foregrounded. A positive effect of Xylitol is his sweetness similar to sucrose however the loss of many negative effects. Here to mention are the noncariogenic properties and it also is a beneficial food ingredient for people suffering from diabetics because it has only a slight effect on the blood sugar level. The market size of xylitol amounts $340 million with a cost of around $4-5 per kilo (Akinterinwa, Khankal, Cirino; 2008).</p> | ||
| + | <p>When xylose is reduced a pentahydroxy sugar alcohol, named xylitol, is received (1, 2). In technical process this is implemented by a hydrogenation reaction of the sugar over nickel catalysts under high temperature and enormous pressure. Here the biosynthetic production is advantageously because of the lower risks for the environment even if the costs for enzyme-based reactions in vitro are still quite high. This would be prevented once the whole cell would be used as a production system. Our focus lies on the attributes of xylitol as a monomer-precursor for plastics. Concerning the hydroxyl-groups of xylitol it is conceivable as a linking element in a polymer with itaconic acid. It is possible according to the variety in hydroxyl-groups that there are more potential ways of cross-linking between the chemicals. This would lead to different textures of the emerging polymer.</p> | ||
| + | <p>Production of xylitol requires one additional gene that is extracted from the host <em>Saccharomyces cerevisiae</em>. The gene <em>GRE3</em> codes for a NADPH-dependent heterologous aldose reductase and is induced under stress conditions in <em>S.cerevisiae</em>. Within our chosen model organism E.coli the conversion of D-xylose-an aldopentose- to xylitol is aimed by reduction of the in D-xylose occurring oxygen double bond.</p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p><img src="filemanager/source/Xylose pathway.jpg" alt="xylitol pathway" width="385" height="431" /></p> | ||
| + | <p class="EndNoteBibliography">1. Akinterinwa O, Khankal R, Cirino PC. Metabolic engineering for bioproduction of sugar alcohols. Curr Opin Biotechnol. 2008;19(5):461-7.</p> | ||
| + | <p class="EndNoteBibliography">2. Akileshwari C, Muthenna P, Nastasijevic B, Joksic G, Petrash JM, Reddy GB. Inhibition of aldose reductase by Gentiana lutea extracts. Exp Diabetes Res. 2012;2012:147965.</p> | ||
| + | <p> </p> | ||
Revision as of 10:32, 16 September 2015
Biotechnological production of Xylitol in Escherichia coli
In the same way itaconic acid can be used to do cross-links due to its various reactive groups, the sugar substitute Xylitol can be used in related applications. Xylitol, as known as Xylit, occurs in slight amounts in several vegetables and in the rind of trees. Because of the dry mass of just 1%, conventional extraction out of these plants has no high economic value. At this point we, the iGEM-Team TU Darmstadt 2015, aim to add an alternative way of producing xylitol using biological fermentation containing specifically genetically modified Escherichia coli.
As a sugar alcohol xylitol is a relevant substance for pharmaceuticals, oral and personal care product and as precursor for chemical synthesis. Because of these applications a microbial based industrial scale production gets more and more foregrounded. A positive effect of Xylitol is his sweetness similar to sucrose however the loss of many negative effects. Here to mention are the noncariogenic properties and it also is a beneficial food ingredient for people suffering from diabetics because it has only a slight effect on the blood sugar level. The market size of xylitol amounts $340 million with a cost of around $4-5 per kilo (Akinterinwa, Khankal, Cirino; 2008).
When xylose is reduced a pentahydroxy sugar alcohol, named xylitol, is received (1, 2). In technical process this is implemented by a hydrogenation reaction of the sugar over nickel catalysts under high temperature and enormous pressure. Here the biosynthetic production is advantageously because of the lower risks for the environment even if the costs for enzyme-based reactions in vitro are still quite high. This would be prevented once the whole cell would be used as a production system. Our focus lies on the attributes of xylitol as a monomer-precursor for plastics. Concerning the hydroxyl-groups of xylitol it is conceivable as a linking element in a polymer with itaconic acid. It is possible according to the variety in hydroxyl-groups that there are more potential ways of cross-linking between the chemicals. This would lead to different textures of the emerging polymer.
Production of xylitol requires one additional gene that is extracted from the host Saccharomyces cerevisiae. The gene GRE3 codes for a NADPH-dependent heterologous aldose reductase and is induced under stress conditions in S.cerevisiae. Within our chosen model organism E.coli the conversion of D-xylose-an aldopentose- to xylitol is aimed by reduction of the in D-xylose occurring oxygen double bond.
<img src="filemanager/source/Xylose pathway.jpg" alt="xylitol pathway" width="385" height="431" />
1. Akinterinwa O, Khankal R, Cirino PC. Metabolic engineering for bioproduction of sugar alcohols. Curr Opin Biotechnol. 2008;19(5):461-7.
2. Akileshwari C, Muthenna P, Nastasijevic B, Joksic G, Petrash JM, Reddy GB. Inhibition of aldose reductase by Gentiana lutea extracts. Exp Diabetes Res. 2012;2012:147965.