Difference between revisions of "Team:NRP-UEA-Norwich/Results"

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     <h1 class="title1">Modelling and Software</h2>
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     <h1 class="title1">Our Results</h2>
 
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     <p class="space20">While we were bringing all the parts together in the lab, a lot of unanswered questions started to raised: How does glycogen and starch look like in real life? How will GlgX and GlgB expression affect the glycogen global structure? How this branching and debranching process take place? And,
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     <p class="space20">Introduce it....</p>
how will the acyltransferases add the acyl group into the glycogen/starch molecule and in which position? Will this addition affect the functionality of the molecule? </p>
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<p>Thanks to computers, software and maths, we were able to predict the outcome of all this processes to help us understanding what was going to happen in the lab. </p>
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     <h3 class="title">Software: Glyco2D</h2>
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     <h3 class="title">Results - Probiotic</h2>
 
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     <p class="space20">Info again...</p>
We used glycogen as a model system to gain a better understanding of the parameters involved in carbohydrate structure, branching and modification. </p>
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<p>We built Glyco2D based on the mathematical model that described the structural properties of glycogen based on different parameters such as chain length, branching degree and the number of tiers<sub><a data-id="ref" class="scroll-link" style = "color: #002bb8;">1</a></sub>. </p>
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        <p class="space20">Then we used the software to model the putative changes in glycogen structure depending on the location of the modification.  Our aim was to produce carbohydrate molecules with 5-10% of the residues modified since this level of butrylation (achieved by chemical modification) has positive benefits to the colons of rats <sub><a data-id="ref" class="scroll-link" style = "color: #002bb8;">2</a></sub>.</p>
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<p class="space20">You can learn more by clicking on the image to the right.</p>
 
<p class="space20">You can learn more by clicking on the image to the right.</p>
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                         <img src="https://static.igem.org/mediawiki/2015/f/fb/NRP-UEA-Norwich-modelling-unmodified-structure.png" class="img-responsive" style="width:400px; height=300px;">
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                         <img src="https://static.igem.org/mediawiki/2015/d/d2/NRP-UEA-Norwich-results-pic.png" class="img-responsive " style="width:375px; height: 375px;">
 
                            
 
                            
                           <a title="Glyco2D"  href="https://2015.igem.org/Team:NRP-UEA-Norwich/Software" class="btn outlinebtn  space50">
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                           <a title="Results - Probiotic"  href="https://2015.igem.org/Team:NRP-UEA-Norwich/Software" class="btn outlinebtn  space50">
 
                             Read More
 
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     <h3 class="title">3D models</h2>
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     <h3 class="title">Results - Prebiotic</h2>
 
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     <p class="space20">We build 3D models of carbohydrates (glycogen and the 2 components of starch -amylopectine and amylose-) to get a better understanding of the effect of branching degree and chain length on the real structure. We used the <href="http://www.glycosciences.de/modeling/sweet2/doc/index.php">SWEET </a> software to build them. </p>
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     <p class="space20">Brief Intro</p>
 
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        <p class="space20">We developed a pipeline to 3D print these molecules. After several attempts, we managed to have our own 3D printed glycogen! </p>
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<p class="space20">You can learn more by clicking on the image on the right.</p>
 
<p class="space20">You can learn more by clicking on the image on the right.</p>
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                           <a title="Results - Prebiotic"  href="https://2015.igem.org/Team:NRP-UEA-Norwich/Modeling/3D" class="btn outlinebtn  space50">
 
                             Read More
 
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Revision as of 11:09, 16 September 2015

House of Carbs

Our Results

Introduce it....

Results - Probiotic

Info again...

You can learn more by clicking on the image to the right.

Results - Prebiotic

Brief Intro

You can learn more by clicking on the image on the right.

MANY THANKS TO OUR SPONSORS

Useful Links

Contact Us

  • nrpuea.igem2015@gmail.com

  • Norwich Research Park,
  • Colney,
  • Norwich, NR4 7UH, UK.

We are the NRP-UEA-Norwich 2015 iGEM Team.

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