Difference between revisions of "Team:HSNU-TAIPEI/projectcadmium"

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<span>Whether Cadmium can enter e.coli or not</span>
 
<span>Whether Cadmium can enter e.coli or not</span>
 
<ol class="article-ol" type="A">
 
<ol class="article-ol" type="A">
−
<li><span>Method</span></li>
+
<li><span>Method</span>
 +
<h4 class="article-h4">Next day</h4>
 +
<ol class="article-ol">
 +
  <li>
 +
    <p class="article-p">Prepare 16 microcentrifuge tubes.(5 kinds of concentration *3 timings+control)</p>
 +
    <p class="article-p">Add 500&#956;l of DH5&#945; to each tube.</p>
 +
    <p class="article-p">Centrifuge all tubes at 4000rpm for 3min.</p>
 +
    <p class="article-p">Remove the supernatent.</p>
 +
  </li>
 +
  <li>
 +
    <p class="article-p">Add 1000&#956;l of the toxic solution each time.</p>
 +
    <p class="article-p">Follow the concentration and 3 timings(0.5hr / 1hr / 1.5hr).</p>
 +
  </li>
 +
  <li>
 +
    <ol class="article-ol">
 +
      <li>Add 0.5cc of ddH<sub>2</sub>O and mix with the bacterias</li>
 +
      <li>Centrifuge at 13000rpm for 30 sec</li>
 +
      <li>Remove the water</li>
 +
      <li>Repeat step1~step3 for three times</li>
 +
    </ol>
 +
  </li>
 +
  <li>
 +
    <p class="article-p">Add 1cc of ddH<sub>2</sub>O and mix with the bacterias</p>
 +
    <p class="article-p">Centrifuge at 13000rpm for 30sec.</p>
 +
    <p class="article-p">Remove 700&#956;l of the supernatant</p>
 +
  </li>
 +
  <li>
 +
    <p class="article-p">Kill the bacteria:</p>
 +
      <ol class="article-ol">
 +
        <li>Put all the tubes in the Liquid nitrogen</li>
 +
        <li>When they freeze,heat them at 100&#8451;</li>
 +
        <li>Repeat step1~step2 for 3 times</li>
 +
      </ol>
 +
  </li>
 +
</ol>
 +
 
 +
</li>
 +
 
 
<li><span>Result</span></li>
 
<li><span>Result</span></li>
 
<li><span>Discussion</span></li>
 
<li><span>Discussion</span></li>

Revision as of 12:51, 16 September 2015

ProjectCadmium

Introduction

  1. Why do we detect cadmium?

    Vegetable oil can be extracted in various ways. But if the plants’ resources are exposed to polluted soil, the vegetable oil may produces toxins. Once polluted or unstandardized oil is mixed with these polluted plants, those plants will contain pollutants. Moreover, we’ve noticed that the problem of cadmium-polluted planet is a common situation all over the world and especially in that of China. Recycled tainted oil will also contain cadmium. As a result, cadmium can be used as a target of detecting tainted oil. Because of this reason, our group wants to detect cadmium.

  2. The harm of cadmium

    Cadmium is also an environmental hazard. Human exposures to environmental cadmium are primarily the result of fossil fuel combustion, phosphate fertilizers, natural sources, iron and steel production, cement production and so on. Breads, root crops, and vegetables also contribute to the cadmium in modern populations. There have been a few instances of general population toxicity as the result of long-term exposure to cadmium in contaminated food and water, and research is ongoing regarding the estrogen mimicry that may induce breast cancer. Some members of the agricultural communities consuming the contaminated rice with cadmium developed itai-itai disease and renal abnormalities, including proteinuria and glucosuria.

  3. Taiwanese regulation
    • 3-1: The limit of cadmium in vegetable or mushroom
      Vegetable: 0.05~0.2ppm ; mushroom: 2ppm
    • 3-2: The limit of cadmium in rice
      Rice: 0.4 ppm
    • 3-3: The limit of cadmium in oil
      There is no law to standard.
  4. National regulations

    Due to the adverse effects on the environment and human health, the supply and use of cadmium is restricted in Europe under the REACH Regulation. The EFSA Panel on Contaminants in the Food Chain has set 2.5 μg/kg body weight as a tolerable weekly intake for humans. In comparison, the Joint FAO/WHO Expert Committee on Food Additives has established 7 μg/kg bw as a provisional tolerable weekly intake level.

    America:

    1. Restrict the release of cadmium to lack, river, arable land, rubbish tip.
    2. The limit of cadmium in water :10ppm
    3. The limit of cadmium in food coloring :15ppm

Circuit Design

Zintp K896008 is a promoter which is designed by NYMU in 2013.It is a part highly specific to the cadmium. When cadmium attaches to Zintp k896008, the circuit will begins. And the fluorescence protein E1010/E0040 receives the message from zintp k896008,It will result in purple and red fluorescence. Finally, the terminator, B0015 will complete the circuit.

Result

  1. Whether Cadmium can enter e.coli or not
    1. Method

      Next day

      1. Prepare 16 microcentrifuge tubes.(5 kinds of concentration *3 timings+control)

        Add 500μl of DH5α to each tube.

        Centrifuge all tubes at 4000rpm for 3min.

        Remove the supernatent.

      2. Add 1000μl of the toxic solution each time.

        Follow the concentration and 3 timings(0.5hr / 1hr / 1.5hr).

        1. Add 0.5cc of ddH2O and mix with the bacterias
        2. Centrifuge at 13000rpm for 30 sec
        3. Remove the water
        4. Repeat step1~step3 for three times

      4. Add 1cc of ddH2O and mix with the bacterias

        Centrifuge at 13000rpm for 30sec.

        Remove 700μl of the supernatant

      5. Kill the bacteria:

        1. Put all the tubes in the Liquid nitrogen
        2. When they freeze,heat them at 100℃
        3. Repeat step1~step2 for 3 times
    2. Result
    3. Discussion
  2. Whether e.coli is alive in the poisons, condition or not
    1. Method
    2. Results
    3. Discussion
  3. The relation between the concentration of Cadmium and illumination of RFP
    1. Method
    2. Results
    3. Discussion

Reference

  • [1] Metal contamination of soils and crops affected by the Chenzhou lead/zinc mine spill (Hunan, China).(Liu H, Probst A, Liao B.)
  • [2] High levels of heavy metals in rice (Oryza sativa L.) from a typical E-waste recycling area in southeast China and its potential risk to human health (Jianjie Fu, Qunfang Zhou, Jiemin Liu, Wei LiuThanh Wang,Qinghua Zhang,Guibin Jiang)
  • [3]Rancidity of Used Cooking Oil and Heavy Metal Analy ses on Selected Street-Vended Foods (Annabelle A. Callano( University of the Immaculate Conception)
  • [4] Cadmium. (2015,august 02) In Wikipedia, the free encyclopedia. Retrieved August 14, 2015, from http://en.wikipedia.org/wiki/cadmium