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<h2 class="title1">COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (CODING SEQUNCES WERE FROM Coprococcus sp. L2-50 (DSM 16842) </h2>
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    <th>Part Contents</th>
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<th>Function</th>
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<tr><td>P-Lac(R0010)_RBS(B0034)_Buk</td><td>pSB1C3</td><td>Butyrate kinase expression contruct </td><td> Catalyzes the conversionbutyrate to/from butyryl</td><td><a href="http://parts.igem.org/Part:BBa_K1618012">BBa_K1618012</a></td></tr>
  
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<tr><td>P-Lac(R0010)_RBS(B0034)_PTB</td><td>pSB1C3</td><td>Phosphotransbutrylase expression construct</td><td>Catalyzes the conversion  butyryl to/from butyryl CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618013">BBa_K1618013</a></td></tr>
  
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  <tr><td>P-Lac(R0010)_RBS(B0034)_Buk_PTB</td><td>pSB1C3</td><td>utyrate to butyryl CoA pathway</td><td> Catalyzes the conversion  butyryl to/from butyryl CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618020">BBa_K1618020</a></td></tr>
  
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<tr><td>P-Lac(R0010)_RBS(B0034)_THL</td><td>pSB1C3</td><td>Thiolase expression construct</td><td>Catalyzes the conversion of Acetyl-CoA to Acetoacetyl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618014">BBa_K1618014</a></td></tr>
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<tr><td>P-Lac(R0010)_RBS(B0034)_CRO</td><td>pSB1C3</td><td>Crotonase expression construct</td><td>Catalyzes conversion of3-hydroxybutyryl-CoA to Crotonyl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618015">BBa_K1618015</a></td></tr>
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<tr><td>P-Lac(R0010)_RBS(B0034)_BHBD</td><td>pSB1C3</td><td>β-hydroxybutyryl-CoA dehydrogenase expression</td><td>Catalyzes conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618016">BBa_K1618016</a></td></tr>
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<tr><td>P-Lac(R0010)_RBS(B0034)_BCD</td><td>pSB1C3</td><td>Butyryl-CoA dehydrogenase expression construct</td><td> Catalyzes conversion of Crotonyl-CoA to Butyryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618017">BBa_K1618017</a></td></tr>
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<tr><td>P-Lac(R0010)_RBS(B0034)_EFTb</td><td>pSB1C3</td><td>Electron transfer protein &#946; expression construct</td><td>Catalyzes conversion of acetate to butyrate</td><td><a href="http://parts.igem.org/Part:BBa_K1618018">BBa_K1618018</a></td></tr>
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<tr><td>P-Lac(R0010)_RBS(B0034)_EFTa</td><td>pSB1C3</td><td>Electron transfer protein &#945; expression construct</td><td>Catalyzes conversion of acetate to butyrate</td><td><a href="http://parts.igem.org/Part:BBa_K1618019">BBa_K1618019</a></td></tr>
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  <tr><td>P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD</td><td>pSB1C3</td><td>Acetyl-CoA to 3-crotonyl-CoA pathway</td><td>Catalyzes conversion of Acetyl-CoA to 3-crotonyl-CoA</td><td>--------</a></td></tr>
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  <tr><td>P-Lac(R0010)_RBS(B0034)_EFTa</td><td>pSB1C3</td><td>Crotonyl-CoA to Butyryl-coA pathway</td><td>Catalyzes conversion of Crotonyl-CoA to Butyryl-coA</td><td>-----------</a></td></tr>
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<tr><td>P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTa</td><td>pSB1C3</td><td>Acetyl-coA to Butryl-CoA pathway</td><td>Catalyzes conversion of Acetyl-coA to Butryl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618021">BBa_K1618021</a></td></tr>
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<tr><td>P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTa</td><td>pSB1C3</td><td>Acetyl coA to butyrate complete biosynthetic pathway</td><td>Catalyzes conversion of Acetyl coA to butyrate</td><td>----------</td></tr>
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</table>
  
  

Revision as of 13:50, 16 September 2015

House of Carbs

Parts

BASIC PARTS FOR ENGINEERING BACTERIAL GLYCOGEN

Part Contents Backbone Description Function Part number
GlgXpSB1C3Debranching enzyme (E.coli)Coding sequence only BBa_K1618025
GlgBpSB1C3Branching Enzyme (E.coli)Coding sequence only BBa_K1618000
MAOpSB1C3Acyl-transferase Candidate 1 Coding sequence only BBa_K1618001
Rv3030pSB1C3Acylation candidate 2Coding sequence onlyBBa_K1618002
Rv3034cpSB1C3Acylation candidate 3Coding sequence only BBa_K1618003
Rv3037cpSB1C3Acylation candidate 4Coding sequence only BBa_K1618004

COMPOUND PARTS FOR ENGINEERING BACTERIAL GLYCOGEN

Part Contents Backbone Description Function Part number
P-Lac(R0010)_RBS(B0034)_GlgX(K1618026)pSB1C3Debranching enzyme expression constructRemoves glycogen branchesBBa_K1618026
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)pSB1C3Branching enzyme expression constructAdd glycogen branchesBBa_K1618022
P-Lac(R0010)_RBS(B0034)_MAO(K1618001)pSB1C3Acyl-transferase expression constructTransfers acyl groups to glycogenBBa_K1618005
P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002)pSB1C3Acyl-transferase expression constructTransfers acyl groups to glycogenBBa_K1618007
P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003)pSB1C3Acyl-transferase expression constructTransfers acyl groups to glycogen BBa_K1618008
P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004)pSB1C3Acyl-transferase expression constructTransfers acyl groups to glycogen BBa_K1618009
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_MAO(K1618001)pSB1C3Branching enzyme + acyl-transferase expression constructTransfers acyl groups to glycogenBBa_K1618023
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002)pSB1C3Branching enzyme + acyl-transferase expression construct)Transfers acyl groups to glycogenBBa_K1618023
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003)pSB1C3Branching enzyme + acyl-transferase expression constructTransfers acyl groups to glycogenBBa_K1618024
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004)pSB1C3BBranching enzyme + acyl-transferase expression constructTransfers acyl groups to glycogenBBa_K1618027

COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (CODING SEQUNCES WERE FROM Coprococcus sp. L2-50 (DSM 16842)

Part Contents Backbone Description Function Part number
P-Lac(R0010)_RBS(B0034)_BukpSB1C3Butyrate kinase expression contruct Catalyzes the conversionbutyrate to/from butyrylBBa_K1618012
P-Lac(R0010)_RBS(B0034)_PTBpSB1C3Phosphotransbutrylase expression constructCatalyzes the conversion butyryl to/from butyryl CoABBa_K1618013
P-Lac(R0010)_RBS(B0034)_Buk_PTBpSB1C3utyrate to butyryl CoA pathway Catalyzes the conversion butyryl to/from butyryl CoABBa_K1618020
P-Lac(R0010)_RBS(B0034)_THLpSB1C3Thiolase expression constructCatalyzes the conversion of Acetyl-CoA to Acetoacetyl-CoA BBa_K1618014
P-Lac(R0010)_RBS(B0034)_CROpSB1C3Crotonase expression constructCatalyzes conversion of3-hydroxybutyryl-CoA to Crotonyl-CoA BBa_K1618015
P-Lac(R0010)_RBS(B0034)_BHBDpSB1C3β-hydroxybutyryl-CoA dehydrogenase expressionCatalyzes conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA BBa_K1618016
P-Lac(R0010)_RBS(B0034)_BCDpSB1C3Butyryl-CoA dehydrogenase expression construct Catalyzes conversion of Crotonyl-CoA to Butyryl-CoA BBa_K1618017
P-Lac(R0010)_RBS(B0034)_EFTbpSB1C3Electron transfer protein β expression constructCatalyzes conversion of acetate to butyrateBBa_K1618018
P-Lac(R0010)_RBS(B0034)_EFTapSB1C3Electron transfer protein α expression constructCatalyzes conversion of acetate to butyrateBBa_K1618019
P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBDpSB1C3Acetyl-CoA to 3-crotonyl-CoA pathwayCatalyzes conversion of Acetyl-CoA to 3-crotonyl-CoA--------
P-Lac(R0010)_RBS(B0034)_EFTapSB1C3Crotonyl-CoA to Butyryl-coA pathwayCatalyzes conversion of Crotonyl-CoA to Butyryl-coA-----------
P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTapSB1C3Acetyl-coA to Butryl-CoA pathwayCatalyzes conversion of Acetyl-coA to Butryl-CoA BBa_K1618021
P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTapSB1C3Acetyl coA to butyrate complete biosynthetic pathwayCatalyzes conversion of Acetyl coA to butyrate----------
Plac-GlgXpSB1C3Debranching enzyme with IPTG inducible PromoterRemoves E. coli glycogen branchesBBa_K1618025 GlgXpSB1C3Debranching enzyme  BBa_K1618026 Plac-GlgBpSB1C3Branching enzyme with IPTG inducible PromoterAdd E. coli glycogen branchesBBa_K1618022 GlgBpSB1C3Branching Enzyme  BBa_K1618000 Plac-MAOpSB1C3Acylation candidate 1 with IPTG inducible PromoterAcylates glycogen? (1)BBa_K1618005 MAOpSB1C3Acylation Candidate 1  BBa_K1618001 Plac-Rv3030pSB1C3Acylation candidate 2 with IPTG inducible PromoterAcylates glycogen? (2)BBa_K1618007 Rv3030pSB1C3Acylation candidate 2 BBa_K1618002 Plac-Rv3034cpSB1C3Acylation candidate 3 with IPTG inducible PromoterAcylates glycogen? (3) BBa_K1618008 Rv3034cpSB1C3Acylation candidate 3  BBa_K1618003 Plac-Rv3037cpSB1C3Acylation candidate 4 with IPTG inducible PromoterAcylates glycogen? (4) BBa_K1618009 Rv3037cpSB1C3Acylation candidate 4  BBa_K1618004 Plac-GlgB-Rv3030pSB1C3Branching enzyme + Acylation candidate 2 with IPTG inducible PromoterMakes acetylated branched glycogen? (2)BBa_K1618023 Plac-GlgB-Rv3034cpSB1C3Branching enzyme + Acylation candidate 3 with IPTG inducible PromoterMakes acetylated branched glycogen? (3)BBa_K1618024 Plac-GlgB-Rv3037cpSB1C3Branching enzyme + Acylation candidate 4 with IPTG inducible PromoterMakes acetylated branched glycogen? (4)BBa_K1618027 Plac-BukpSB1C3Butyrate kinase with IPTG inducible PromoterReversible reaction that makes butyrate from butyryl phosphateBBa_K1618012 Plac-PTBpSB1C3Phosphotransbutrylase with IPTG inducible PromoterReversible reaction that makes butyryl-phosphate from butyryl-CoABBa_K1618013 Plac-THLpSB1C3Thiolase with IPTG inducible PromoterMakes Acetoacetyl-CoA from Acetyl-CoABBa_K1618014 Plac-CROpSB1C3Crotonase with IPTG inducible PromoterMakes Crotonyl-CoA from 3-hydroxybutyryl-CoABBa_K1618015 Plac-BHBDpSB1C3β-hydroxybutyryl-CoA dehydrogenase with IPTG inducible PromoterMakes 3-hydroxybutyryl-CoA from Acetoacetyl-CoA BBa_K1618016 Plac-BCDpSB1C3Butyryl-CoA dehydrogenase with IPTG inducible PromoterMakes Butyryl-CoA from Crotonyl-CoABBa_K1618017 Plac-EFTbpSB1C3Electron transfer protein β with IPTG inducible PromoterMakes butyrate from acetate part 5BBa_K1618018 Plac-EFTapSB1C3Electron transfer protein α with IPTG inducible PromoterMakes butyrate from acetate part 6BBa_K1618019 Plac-Buk-PTBpSB1C3Butyrate to butyryl CoA pathway with IPTG inducible Promoter Reversible reaction that makes Butyrate from Butyryl-CoABBa_K1618020 Plac-THL-CRO-BHBD-BCD-EFTb-EFTapSB1C3Acetyl-CoA to Butyrate pathway with IPTG inducible Promoter Makes butyrate from Acetyl-CoA fullBBa_K1618021 35S-MAO?Acylation candidate 1 with 35S PromoterAcetylates starch? (1)BBa_K1618033 35S-Rv3030?Acylation candidate 2 with 35S PromoterAcetylates starch? (2)BBa_K1618034 35S-Rv3034c?Acylation candidate 3 with 35S PromoterAcetylates starch? (3)BBa_K1618035 35S-Rv3037c?Acylation candidate 4 with 35S PromoterAcetylates starch? (4)BBa_K1618036 35S-GFP-MAO?Acylation candidate 1 with 35S Promoter + GFPAcetylates starch? (1) + GFP tagBBa_K1618029 35S-GFP-Rv3030?Acylation candidate 2 with 35S Promoter + GFPAcetylates starch? (2) + GFP tagBBa_K1618030 35S-GFP-Rv3034c?Acylation candidate 3 with 35S Promoter + GFPAcetylates starch? (3) + GFP tagBBa_K1618031 35S-GFP-Rv3037c?Acylation candidate 4 with 35S Promoter + GFPAcetylates starch? (4) + GFP tagBBa_K1618032 Chloroplast Transit Peptide? Chloroplast Transit PeptideBBa_K1618028 35s Terminator? TerminatorBBa_K1618037

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