Difference between revisions of "Team:LZU-China/chz team"

 
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           <a href=" https://2015.igem.org/Team:LZU-China/chz_team">Team</a>
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           <a href=" https://2015.igem.org/Team:LZU-China/chz_safety">Safety</a>
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           <a href="https://2015.igem.org/Team:LZU-China/chz_safety">Safety</a>
 
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           <a href=" https://2015.igem.org/Team:LZU-China/chz_notes">Notes</a>
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          <a href="https://2015.igem.org/Team:LZU-China/chz_collaborations">Collaboration</a>
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        <h1 class="header center teal-text text-lighten-2">Our Team</h1>
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        <div class="row center">
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          <h5 class="header col s12 light">A Novel Microbial Fuel Cell System that meaxure the content of estrogen</h5>
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          <h5 class="center-align">&nbsp;</h5>
 
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          <h2 class="left-align">This is our Project<sup>[2]</sup></h2>
 
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          <h5 class="left-align">(subtitle)this is our principle</h5>
 
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          <h3 class="center-align">&nbsp;</h3>
 
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        <li class="tab col s3"><a class="active" href="#attri">Attribution</a></li>
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        <li class="tab col s3"><a href="#acknow">Acknowledgements</a></li>
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      <h4>&nbsp;</h4>
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        <thead>
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                <th>icons</th>
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                <th>meaning</th>
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            </tr>
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            </thead>
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            <tbody>
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                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/c/c9/Lzu_china_2015_Hardwaredesign.png"></td>
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                <td>Hardware Design</td>
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            </tr>
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            <tr>
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                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/a/ac/Lzu_china_2015_Softwaredesign.png"></td>
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                <td>Software Design</td>
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            </tr>
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            <tr>
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                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Artdesign.png"></td>
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                <td>Art Design</td>
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            </tr>
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            <tr>
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                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png"></td>
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                <td>MFC experiment</td>
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            </tr>
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                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/2/25/Lzu_china_2015_Devicerunning.png"></td>
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                <td>Device running</td>
 +
            </tr>
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            <tr>
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                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/1/13/Lzu_china_2015_Experiment_design.png"></td>
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                <td>Experiment Design</td>
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            </tr>
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            <tr>
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                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png"></td>
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                <td>HumanPractice</td>
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            </tr>
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                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png"></td>
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                <td>PaperWork</td>
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            </tr>
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            <tr>
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                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png"></td>
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                <td>Part construction</td>
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                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png"></td>
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                <td>Part Examination</td>
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            </tr>
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                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/b/b0/Lzu_china_2015_Teammanagement.png"></td>
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                <td>Team management</td>
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                <td><img style="width:80px" src="https://static.igem.org/mediawiki/2015/3/35/Lzu_china_2015_Wikicode.png"></td>
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                <td>Wiki Code</td>
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            </tr>
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            </tbody>
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  <div class="col s12 m12 l6">
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      <h4>Xiao Zhang</h4>
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      <p>Xiao Zhang majoring in bioinformatics and microbiology is a master degree candidate studying in State Key Laboratory of Grassland Agro-ecosystem, Lanzhou University. As the captain of our team and the co-inventor of our novel device, he conceived and designed the entire MFC system. His versatility has great value for us to finish our project. He is really keen on science research, especially interested in functional genomics. So far, he has taken part in several research topics, and their scientific achievements were reported in 3 different SCI papers.
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      </p>
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        <p>ATTRIBUTION:</p>
 +
        <div class="col s12">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/c/c9/Lzu_china_2015_Hardwaredesign.png">
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<img style="width:50px" src="https://static.igem.org/mediawiki/2015/a/ac/Lzu_china_2015_Softwaredesign.png">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/2/25/Lzu_china_2015_Devicerunning.png">
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          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b0/Lzu_china_2015_Teammanagement.png">
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          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
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          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/1/13/Lzu_china_2015_Experiment_design.png">
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          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
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    </div>
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      <h1>&nbsp;</h1>
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      <ul>
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      <li>OverGraduate</li>
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      <li>UnderGraudate</li>
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      </ul>
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  </div>
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       <h1>&nbsp;</h1>
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        <h4 class="left-align">Background</h4>
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      <li>UnderGraudate</li>
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        <h5 class="left-align">Water pollution</h5>
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          <p> Water, such a vital material for all kinds of creatures, plays a rather important role in human’s life. In China, Laozi, a well-known philosopher in the Spring and Autumn Period, thought highly of water and said, <br/>
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  <div class="col s12 m12 l6">
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‘The best of men is like water;<br/>
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    <div class="row">
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　　Water benefits all things.<br/>
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      <h4>Qi Wu</h4>
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　　And does not compete with them.<br/>
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      <p>Chariness patience and happiness, she is Qi wu, a second times iGEMer. As co-captain this year, She is in charge of logistics and finance. This is a totally different experience from last year and she learned a lot.
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　　It dwells in (the lowly) places that all disdain -<br/>
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      </p>
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              Wherein it comes near to the Tao.’<sup>[1]</sup><br/>
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      <br/>
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However, in our daily life, although water is noble, it is not so strong and usually gets sick. With the development of industry and modern society, water is badly polluted. So what is water pollution? Water pollution happens when toxic substances enter water bodies and degrade the quality of water. Although over 70% of the earth is covered by water, fresh water, which can be used by humans, occupied only 2.53% of the global water resources. Over 80% of rivers in China have been polluted in some degree<sup>[2]</sup>
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          <p>ATTRIBUTION:</p>
 +
          <div>                   
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
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        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b0/Lzu_china_2015_Teammanagement.png">
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        </div>
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    </div>
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  </div>
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    <div class="col s12 m12 l4">
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    <img src="https://static.igem.org/mediawiki/2015/a/a9/Lzu_china_2015_Lzuwuqi.jpg
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      <h4>Haotian Wang</h4>
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              <div class="col s12 m6 l 6"><img src="https://static.igem.org/mediawiki/2015/e/e3/Lzu_china_2015_Backgrondw4.jpg
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      <p>Haotian Wang is a junior student majoring in bioscience. He is a fan of Liverpool F. C. Being a good striker used to be his dream while he is now a sideback. He is so interested in synthetic biology that he always reads some articles before watching games. He constructed some of our parts.
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       </p>
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          <h6>Some picture about water pollution reference from the Internet.</h6>
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          <p>Water pollution can be classified into organic, inorganic and so on. What we care most is heavy metal pollution because heavy metal waste water is the current outstanding problem in the field of water pollution and becomes the focus of research in the controlling field.
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          </p>
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          <p>Heavy metals from industrial processes can accumulate in nearby water body, which are toxic to marine life such as fish and shellfish, and subsequently to the humans who eat them. Heavy metals can slow development, result in birth defects and some are carcinogenic. The most commonly encountered toxic heavy metals in wastewater include Arsenic, Lead, Mercury, Cadmium, and the less common include Chromium, Copper, Nickel, Zinc<sup>[3]</sup>. Three kinds of heavy metals are of concern, including toxic metals (such as Hg, Cr, Pb, Zn, Cu, Ni, Cd, As, Co, Sn, etc.), precious metals (such as Pd, Pt, Ag, Au, Ru etc.) and radionuclides (such as U, Th, Ra, Am, etc.)<sup>[4]</sup>. Heavy metals bring about serious environmental pollution, threatening human health and ecosystem.<sup>[5]</sup>
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          </p>
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          <p>here are millions people are struggling with water pollution. In China, for example, about 75 percent of the population (or 1.1 billion people) are without access to unpolluted drinking water, according to standards from China<sup>[6]</sup>. And our university, Lanzhou University, located on northwestern China, where lacking water especially. What’s worse, water pollution is also serious including heavy metal contaminants.
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          </p>
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        <h5 class="left-align">MFC</h5>
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          <p>
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              Instead of exploiting more water resources, which has more and more negative influence[1], it’s better to make less pollution and treat water pollution.[2] And we must know what kinds of contaminations in the water and how much are them. So we chose MFC (Microbial Fuel Cells) to detect the heavy metal in water.
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          </p>
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          <p>So, what is MFC?
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          </p>
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          <p>Microbial fuel cells (MFCs) provide new opportunities for the sustainable production of energy from biodegradable, reduced compounds. MFCs function on different carbohydrates but also on complex substrates present in wastewaters，which is an ideal approach to solve both pollution problem and energy crisis. Compared with traditional chemical methods of sewage treatment, MFC is environmental friendly and widely adept by various condition<sup>[3]</sup>.
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          </p>
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          <p>A MFC converts biomass energy directly into electricity. This can be achieved when bacteria switch from the natural electron acceptor, such as oxygen or nitrate, to an insoluble acceptor, such as the MFC anode. A typical microbial fuel cell consists of two compartments: anode and cathode compartments separated by a proton exchange membrane (PEM). In the anode compartment, microorganisms oxidize the fuel and generating CO<sub>2</sub>, electrons and protons. Electrons are transferred through an external electric circuit to the cathode compartment, while protons are transferred to the cathode compartment through the membrane. Electrons and protons are consumed in the cathode compartment, combining with oxygen to form water.
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          <div class="col s12">
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          <img src="https://static.igem.org/mediawiki/2015/a/ab/Lzu_china_2015_Mfc-f1.png
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"/ class="responsive-img">
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              <h6>Figure 1. The working principle of a microbial fuel cell. Substrate is metabolized by bacteria, which transfer the gained electrons to the anode. This can occur either directly through the membrane or via mobile redox shuttles. MED, redox mediator; Red oval, terminal electron shuttle in or on the bacterium.<sup>[4]</sup>
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</h6>
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          </div>
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          <p>
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              After electron generated, the electron need to be transferred out of the microbe, The mechanism includes two methods:<br/>
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1.Direct transfer mechanism: Electrons are directly transferred from the microbe’s cell membrane to the anode surface（A）、nanowires transfer the electron through conductive appendages, termed “nanowires”, grown by the bacteria.（B）<br/>
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          2.Indirect transfer mechanism: Microbes employ a secondary biomolecule——electron transfer mediators（ETMs） to shuttle the electron to the anode（C）.
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              <h6>Figure2: Schematic diagram of extracellular electron transfer mechanisms for electrochemically active microorganisms in anode@
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              </h6></div>
+
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          <p>Although MFC is high efficiency of power output and environment friendly, most of the power generation MFCs are still in laboratory and not come into massive scale of industry.  
+
−
</p>
+
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          <P>One major drawback is the output power density is far less to adapt the standard in industry. electron transfer rate is determined by the potential difference, the reorganization energy and electron donor and receptor distance, main factors to determine the output power density of the microbial fuel cell is related to the electron transfer process, that is to say, the biological system of slow electron transfer rate is the bottleneck of the development of microbial fuel cell.  
+
−
</P>
+
−
         
+
−
          <ul>
+
−
              <li>1. Hou-gui, Z., Environmental issues and countermeasures in exploiting water resources of rivers. Journal of Chongqing University 2006. 5(2): p. 111-114.</li>
+
−
<li>2. Rabaey, K. and W. Verstraete, Microbial fuel cells: novel biotechnology for energy generation. Trends in Biotechnology, 2005. 23(6): p. 291-298.</li>
+
−
<li>3. Liu, L., et al., Electron Transfer Mediators in Microbial Electrochemical Systems. PROGRESS IN CHEMISTRY, 2014(11): p. 1859-1866.</li>
+
−
<li>4. Chouler, J. and M. Di Lorenzo, Water Quality Monitoring in Developing Countries; Can Microbial Fuel Cells be the Answer? Biosensors, 2015. 5(3): p. 450.</li>
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−
              </ul>
+
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        <h5 class="left-align">Riboflavin</h5>
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          <p>Riboflavin, a yellow-orange solid substance with poor solubility in water, was originally recognized as a growth factor in 1879 and named vitamin B, according to the British nomenclature system.
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−
          </p>
+
−
          <p>
+
−
          Its IUPAC name is 7,8-Dimethyl-10-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]benzo[g]pteridine-2,4-dione<sup>[8]</sup>. Riboflavin has two active coenzyme forms, riboflavin 5'-phosphate (R5P; flavin mononucleotide [FMN]) and flavin adenine dinucleotide (FAD)<sup>[9]</sup>, which can both transfer electron.
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</p>
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          <div class="col s12">
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              <div class="col s4"><img src="https://static.igem.org/mediawiki/2015/a/a2/Lzu_china_2015_Rib-f1-1.png
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"/></div>
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              <div class="col s4"><img src="https://static.igem.org/mediawiki/2015/2/2a/Lzu_china_2015_Rib-f1-2.png
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              <div class="col s4"><img src="https://static.igem.org/mediawiki/2015/8/89/Lzu_china_2015_Rib-f1-3.png
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"/></div>
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          </div>
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          <h6>Fig 1 Riboflavin: C17H20N4O6. Reference: https://en.wikipedia.org/wiki/Riboflavin</h6>
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          <p>
+
−
          As riboflavin’s structure shows, there is a transfer of two electrons from hydrogen and hydrid ions, so riboflavin can be regarded as a electron shuttle and even a kind of redox mediator. Though riboflavin is very important in mediator-driven bioelectricity generation, the detailed mechanism on electron transfer has not been precisely investigated yet<sup>[10]</sup>.
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−
</p>
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−
          <p>
+
−
          Researchers find that increase of riboflavin biosynthesis can profounfly enhance extracellular electron transfer in bacteria and then improve the efficience of MFC. For example, the increase of riboflavin biosynthesis by Shewanella at the alkaline condition underlies the improvement of the electricity output in MFCs(Fig 2)<sup>[11]</sup>.
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</p>
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          <div class="col s12">
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          <img src="https://static.igem.org/mediawiki/2015/a/ab/Lzu_china_2015_Mfc-f1.png
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"/ class="responsive-img"></div>
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       </div>
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        <h6>
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            Fig 2  Increased riboflavin synthesis underlies enhanced electron transfer in Shewanella. We draw the conclusion that increased riboflavin synthesis can improve the MFC’s production.</h6>
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        <p>
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        So riboflavin became our star. Since it can be expressed in bacteria, we can combine riboflavin and MFC to improve the electricity output of MFC. And it is easy and mature to measure the concentration of rirboflavin in aqueous solution based on the technique of OD (optical density). The peaks of absorbance occur at 223, 266, 373 and 445 nm(Fig 3)<sup>[12]</sup>. P. Drossler etc. investigated a wide range of pH values from pH -1.1 to pH  13.4 and made the absorption spectrum curve over different pH value(Fig 4)<sup>[13]</sup>.
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</p>
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        <div class="col s12">
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        <img src="https://static.igem.org/mediawiki/2015/5/57/Lzu_china_2015_Rib-f3.png
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        <h6>
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            Fig 3 Absorption spectrum of riboflavin in aqweous solution. And the peaks of absorbance occur around at 223, 266, 373 and 445 nm</h6>
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 +
        <p>ATTRIBUTION:</p>
 
         <div class="col s12">
 
         <div class="col s12">
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        <img src="https://static.igem.org/mediawiki/2015/5/58/Lzu_china_2015_Rib-f4.png
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          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
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          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
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          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/1/13/Lzu_china_2015_Experiment_design.png">
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      <h6>
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          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
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            Fig 4 Absorption cross-section spectra of riboflavin in aqueous solutions at various values of pH. The riboflavin concentration used in the measurements was around 10-4 mol dm<sup>3</sup> </h6>
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<p> In our laboratory, we used the multi-spectral scanner to analyse the optimal absorption wavelength and finally found absorption peak at 444nm is the optimum in pH 1.17. And our optimal absorption wavelength corresponds with previous researches. In order to make pH 1.17, we mixed 2ml 0.1mol/L HCl with 1ml riboflavin aqueous solutions extract from engineering bacteria. Then it is able to detect the concentration of rirboflavin by OD value.</p>
+
−
    <p>There are many methods to get riboflavin by microorganism. In E.coli, a gene cluster, consisting of RibA, RibB, RibDG, RibH and RibE and separating to different transcription units, regulates the synthesis of riboflavin. The details of riboflavin biosynthesis have been figured out(Fig 5)<sup>[14]</sup>.
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        </p>
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        <div class="col s12">
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        <img src="https://static.igem.org/mediawiki/2015/d/dd/Lzu_china_2015_Rib-f5.png
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" class="responsive-img" /></div>
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            Fig 5 Biosynthesis of riboflavin and flavocoenzymes from Fischer, 2006 [10]. Step A, GTP cyclohydrolase III; step B, GTP cyclohydrolase II; step C, 2-amino-5- formylamino-6-ribosylamino-4(3H)-pyrimidinone 5’-phosphate hydrolase; step D, 2,5- diamino-6-ribosylamino-4(3H)-pyrimidinone 5’-phosphate deaminase; step E, 5- amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5’-phosphate reductase; step F, 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5’-phosphate reductase; step G, 2,5- diamino-6-ribitylamino-4(3H)-pyrimidinedione 5’-phosphate deaminase; step H, hypothetical phosphatase; step I, 3,4-dihydroxy-2-butanone 4-phosphate synthase; step J, 6,7-dimethyl-8-ribityllumazine synthase; step K, riboflavin synthase; step L, flavokinase; step M, FAD synthetase; 1, GTP; 2, 2,5-diamino-6-ribosylamino-4(3H)- pyrimidinone 5’-phosphate; 3, 2-amino-5-formylamino-6-ribosylamino-4(3H)- pyrimidinone 5´-phosphate; 4, 5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5’-phosphate; 5, 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5’-phosphate; 6, 5- amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5’-phosphate, 7; 5-amino-6- ribitylamino-2,4(1H,3H)-pyrimidinedione; 8, ribulose 5-phosphate; 9, 3,4-dihydroxy-2- butanone 4-phosphate; 10, 6,7-dimethyl-8-ribityllumazine; 11, riboflavin; 12, FMN; 13, FAD.
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−
           
+
−
 
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−
        <p>
+
−
        Green arrows mark the plant pathway; red, fate of the four-carbon precursor 9 derived from ribulose 5-phosphate.
+
−
</p>
+
−
        <blockquote>
+
−
            <ul>
+
−
                <li>1.    Laozi, Tao Te Ching. 516 BC. 8.</li>
+
−
                <li>2.    Qi, F., C. Guodong, and M. Masao, Water Resources in China: Problems and Countermeasures. Ambio, 1999. 28(2): p. 202-203.</li>
+
−
                <li>3.    Heavy metals in wastewater. 2013.</li>
+
−
                <li>4.     Wang, J. and C. Chen, Biosorption of heavy metals by Saccharomyces cerevisiae: A review. Biotechnology Advances, 2006. 24(5): p. 427-451.</li>
+
−
                <li>5.    Wang, J. and C. Chen, Biosorbents for heavy metals removal and their future. Biotechnology Advances, 2009. 27(2): p. 195-226.</li>
+
−
                <li>6.    Hanson Ii, J.R., The World Economy: A Millennial Perspective (Book). Journal of Economic Literature, 2002. 40(4): p. 1256-1257.</li>
+
−
                <li>7.    Hou-gui, Z., Environmental issues and countermeasures in exploiting water resources of rivers. Journal of Chongqing University, 2006. 5(2): p. 111-114.</li>
+
−
                <li>8.    PubChem 493570.</li>
+
−
                <li>9.    Riboflavin. Alternative Medicine Review, 2008. 13(4): p. 334-340.</li>
+
−
                <li>10.   Jung, S.-H., et al., Impedance and Thermodynamic Analysis of Bioanode, Abiotic Anode, and Riboflavin-Amended Anode in Microbial Fuel Cells. Bulletin of the Korean Chemical Society, 2012. 33(10): p. 3349-3354.</li>
+
−
                <li>11.    Yong, Y.C., et al., Increase of riboflavin biosynthesis underlies enhancement of extracellular electron transfer of Shewanella in alkaline microbial fuel cells. Bioresour Technol, 2013. 130: p. 763-8.</li>
+
−
                <li>12.    Lu, C., et al., Photophysical and photochemical processes of riboflavin (vitamin B2) by means of the transient absorption spectra in aqueous solution. Science in China Series B: Chemistry, 2001. 44(1): p. 39-48.</li>
+
−
                <li>13.    Drössler, P., et al., pH dependence of the absorption and emission behaviour of riboflavin in aqueous solution. Chemical Physics, 2002. 282(3): p. 429-439.</li>
+
−
                <li>14.   Kim, R., Biosynthesis of Vitamin B2 (Riboflavin) Studies on the Reaction Mechanism of Riboflavin Synthase. 2012.</li>
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−
            </ul>
+
−
        </blockquote>
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      <h4>&nbsp;</h4>
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      <h1>&nbsp;</h1>
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      <div id="popout" class="col s12">
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      <ul>
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        <h4 class="left-align">Wet Lab</h4>
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      <li>OverGraduate</li>
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          <div class="progress">
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      <li>UnderGraudate</li>
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          <div class="determinate" style="width: 0%"></div>
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      </ul>
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  </div>
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        <h5 class="left-align">Introduction</h5>
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</div><!--L3-->
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        <p>
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          As it is known to all, Sherlock Holmes is a famous detective. Based on synthesis biology, our team creates a smart system called Micro Holmes which can monitor the pollutant inside the water in real time. Once the system was founded, through users’ laptops, smartphones or some other media, we would acquire the statistics of pollutant which has been analyzed by the system. Reforming the E.Coli, we endowed them with the ability of sensing the pollutant inside the water and delivering the message of it. Therefore, raising them inside the Microbial Fuel Cell (MFC), they can rapidly augment the voltage as soon as the polluted occurred. Then, by the automatically collecting and analyzing equipment, we can know the concentration of pollutant converted by the instant electric signal. Escherichia coli, a tiny but full of power creature, perfectly plays an important part as Holmes in our project. So, are you interest in how he was created and how he works?
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          </p>
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          <p>First of all，Mr. Holmes must has a piercing eye to find the trail of pollutant; And after detecting pollutant, Mr. Holmes must has the ability to convey the message to us in time. Therefore, in order to constitute a series of inductivity parts—which can be efficiently expressed in E.Coli BL 21 bacterial strains—we link the efficient receptor of heavy metal ions with RibB--- the key synthesis gene of riboflavin. They can translate the information from the concentration of heavy metal ions to the riboflavin content, which is the product of reporter gene RibB. Riboflavin is a kind of redox mediator who works as a highly-efficient electronic transfer mediator. When the concentration of Riboflavin increased, it can improve the efficiency of electronic translocation between E.Coli and the anode and eventually augment the voltage. Combined with MFC (microbial fuel cell) system, we can measure the concentration of heavy metal ions in water through the voltage presented by MFC system and realize the conversion of heavy metal ions concentration to electrical signal. This method has lots of advantages, such as compatibility, high efficiency, instantaneity, reliability and so on.</p>
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        <p>The main idea of designing this experiment can be seen in the following flow chart.</p>
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        <img src="https://static.igem.org/mediawiki/2015/f/fa/Lzu_china_2015_Figure1.png
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      </div>
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      <h6>Fig 1 Whole Experiment Flow Chart </h6>
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        <p>In this project, parts were divided into two kinds. One is constitutive part, combined by various type of promoter, RBS, RibB and terminator. We constructed the constitutive parts to select a highly-expressed part of RibB. The other is inducible part with RBS, RibB terminator and promoter which can sense heavy metal ions. This kind of parts can highly express RibB so that it is practical to associate the concentration of heavy metal ions with the output of riboflavin. Therefore, putting the E.Coli BL 21 into the MFC, we collected the statistics of MFC on the condition that concentration of metal ion solution was already known. So we built the correlation model, then induced the E.Coli BL21 with the solution to be measured. It is easy to figure out the concentration by measuring the voltage of MFC. We can monitor the specific metal ions real-timely, quantitatively and swiftly through this way and more importantly, it has compatibility in the measurement of metal ion by changing the parts to detect different metal ions.</p>
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        <h4>&nbsp;</h4>
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        <h5 class="left-align">An efficient riboflavin synthesis gene: <em>RibB</em></h5>
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      <h1>&nbsp;</h1>
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        <h6 class="left-align">1 An Introduction of <em>RibB</em></h6>
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      <ul>
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        <h6 class="left-align">1.1 The biosynthesis of riboflavin inside <em>E.coli</em></h6>
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      <li>OverGraduate</li>
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        <p>
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      <li>UnderGraudate</li>
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          <a href="#" title="23333">Riboflavin</a>, also <a href="#" id="ref1" onmouseover="over()" onmouseout="out()"> called</a> vitamin B2, can be synthesized by various kinds of plants and animals. The basic synthesis principal is similar, that one molecular of GTP and two molecular of ribulose 5-phosphate undergo a series of enzymatic reaction[1]. However, the operons vary from bacteria to bacteria. At present, we have made the principal clear in most kind of bacterium and in this project, we chose E.coli in which the riboflavin synthetic genes emerge as nonlinear gene cluster and was distributed into nonhomologous chromosome.
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  <div class="col s12 m12 l6">
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          Fig 2-A shows the process of riboflavin synthesis and its relevant genes including RibA (coding GTP cyclohydrolase II), RibB (coding 3,4-dihydroxy-2-butanone-4-phosphate synthase), RibDG (coding a kind of bifunctional riboflavin specificdeaminase/reductase), RibH (coding lumazine synthase) and RibE (coding riboflavin synthase). The distribution of these gene can be seen in Fig 2-B[2].
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    <div class="row">
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        </p>
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      <h4>Xuguo Wang</h4>
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        <img src="https://static.igem.org/mediawiki/2015/4/4e/Lzu_china_2015_Figure2A1.png
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      <p>Xuguo,Wang.As people say, guy who learning computer always a geek, yes, he is.
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He is a graduate student of Lanzhou University, he is learning linux kernel,
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        <img src="https://static.igem.org/mediawiki/2015/7/70/Lzu_china_2015_Figure2A2.png
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besides this, he likes making some interesting electronical device, and like Quadcopter,
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like transform the Raspberry Pi to control his home's something. Well, that is very
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        <p>Fig 2-A The riboflavin biosynthesis inside Escherichia coli[2].</p>
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interesting. He is dreaming to contribute the linux kernel code, and working
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for this. He is a keen on computer algorithm, and hope someday, can make his own
−
        <img src="https://static.igem.org/mediawiki/2015/3/39/Lzu_china_2015_Figure2B.png
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algorithm.
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        <p>Fig 2-B RibA, RibB, RibDG, RibH and RibE inside the E.coli are not on the same transcription unit but separate to different transcription units which have different promoters. Genes in yellow are related to the synthesis of riboflavin while the grey are not known to be involved in flavin biosynthesis[2].</p>
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        <h6 class="left-align">1.2 <em>RibB</em> and its function</h6>
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        <p>The synthesis of riboflavin is complicated because there are so many genes involved in this process. Besides, those genes are not focus on a same operon. According to some research, RibB is of vital importance to the synthesis of riboflavin which in charge of coding DHBP synthetase and can catalyze the synthesis of 3,4-dihydroxy-2-butanone-4-phosphate[3]. Researches conducted by Danielle Pedrolli and other researchers show that the FMN riboswitch, which is in the untranslated region of 5’ terminal of RibB, can regulate the expression of RibB so that it can inhibit the biosynthesis of riboflavin. Thus, the enzyme translated by RibB becomes a limiter of riboflavin synthesis. Therefore, we can evidently improve the output of riboflavin by knocking out the FMN riboswitch or overexpressing RibB. Related experiment statistics can be referred from Fig 3. Compared with the whole riboflavin synthesis genes cluster, RibB has the advantages of convenience, high efficiency and short sequence as it is only 654bp. Consequently, our team choosed RibB as a report gene to construct parts and then builded the connection between heavy metal ions and RibB and finally used the product of RibB—riboflavin to impact the voltage of MFC so that we realized the monitoring of heavy metal ions automatically, quantitatively and efficiently.
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        </p>
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        <img src="https://static.igem.org/mediawiki/2015/2/26/Lzu_china_2015_Figure3A.png
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        <img src="https://static.igem.org/mediawiki/2015/4/44/Lzu_china_2015_Figure3B.png
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        <p>Fig 3 The expression of riboflavin increased[2].
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Fig 3-A After knocking out the FMN riboswitch, the expression of riboflavin increased. Abbreviation “del”, the test tube on the right side, is the E.coli knocked out the FMN riboswitch and the pNCO113 is control.<br />
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Fig 3-A After knocking out the FMN riboswitch, the expression of riboflavin increased. Abbreviation “del”, the test tube on the right side, is the E.coli knocked out the FMN riboswitch and the pNCO113 is control.<br />
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Fig 3-B After overexpressing RibB, the expression of riboflavin increased. “ribB”, the test tube on the right side, is the E.coli overexpressed RibB and the pNCO113 is control.
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</p>
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        <h6 class="left-align">2 The measurement data of several constitutive parts</h6>
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        <p>Totally, we constructed 5 kinds of constitutive parts and quantitatively measured their ability of producing of riboflavin. The character of these 5 parts can be seen in Table 1.
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Table 1 Part of the message of measured part
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</p>
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        <table class="responsive-table striped">
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        <thead>
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          <tr>
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              <th data-field="id">NO.</th>
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              <th data-field="name">Construction</th>
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        </thead>
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        <tbody>
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          <tr>
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            <td>K1755005</td>
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            <td>K608003(strong promoter +medium RBS)+ ribB + B0014(terminator)</td>
+
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          </tr>
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          <tr>
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            <td>K1755006</td>
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            <td>K608006(medium promoter + medium RBS) + ribB + B0014(terminator)</td>
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          </tr>
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          <tr>
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            <td>K1755007</td>
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            <td>K608002(strong promoter +strong RBS) + ribB + B0014(terminator)</td>
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          </tr>
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          <tr>
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            <td>K1755008</td>
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            <td>K608007(medium promoter +weak RBS) + ribB + B0014(terminator)</td>
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          </tr>
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          <tr>
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            <td>K1755009</td>
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            <td>K608004(strong promoter +weak RBS) + ribB + B0014(terminator)</td>
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          </tr>
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        </tbody>
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      </table>
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      <p>
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        We cultured the transgened E.coli BL21 in M9 colorless culture media while at the same time set up a control with the original BL21. Compared with the control, it is seemingly that the BL21 with RibB gene part can product more riboflavin (riboflavin solution looks yellow), seeing Fig 4.
+
 
       </p>
 
       </p>
−
       <img src="https://static.igem.org/mediawiki/2015/3/31/Lzu_china_2015_Figure4.jpg" class="responsive-img">
+
       <br/>
−
      <p>Fig 4 From left to right, respectively, the solution is BL21 control, BL21 with K1755005, BL21 with K1755006, BL21 with K1755009.<br />
+
        <p>ATTRIBUTION:</p>
−
Use spectrophotometer to measure the concentration of bacteria and riboflavin at different time. The consequence of the experiment can be seen in Fig 5.
+
        <div>             
−
</p>      <div class="col s12"> <div class="col s12 m6 l6">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/c/c9/Lzu_china_2015_Hardwaredesign.png">
−
          <canvas id="figure5a" height="250px"></canvas>
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/a/ac/Lzu_china_2015_Softwaredesign.png">
−
          <h6 class="center-align">Figure5-A</h6>
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/2/25/Lzu_china_2015_Devicerunning.png">
 +
<img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
 
         </div>
 
         </div>
−
        <div class="col s0 m1 l1">&nbsp;</div>
+
    </div>
−
        <div class="col s12 m5 l5"><p>Fig 5-A Bacteria concentration of  K1755005, K1755006, K1755009 bacterial strains vary with time.
+
  </div>
−
</p></div></div>
+
    <div class="col s12 m12 l4">
−
   
+
    <img src="https://static.igem.org/mediawiki/2015/9/96/Lzu_china_2015_Lzuwangxuguo.jpg
−
     
+
" class="responsive-img avatr">
−
      <p>
+
  </div>
−
        Concentration of the microbial strains of  K1755005, K1755006, K1755009 bacterial strains basically don't have difference at the same point in time, and the corresponding statistical analysis also showed that there are no significant differences between the three parts(the results are shown in Table 2). It shows that when bacterial strains are linked with K1755005, K1755006 or K1755009, the influence to the viability of the bacteria is very limited and can even ignore it. That's why the concentration of the microbial strains is basically the same with the different processing at the same time. In the following research we can directly compare the concentration of riboflavin to analyze the ability of riboflavin synthesis in different bacterial strains.
+
 
 +
</div><!--R4-->
  
 +
<div class="col s12" style="background-color: rgb(189, 212, 253);">
 +
  <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/d/dc/Lzu_china_2015_Lzuchenzhengjun.jpg
 +
" class="responsive-img avat">
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Zhengjun Chen</h4>
 +
      <p>(1985- ), male, is studying at Lanzhou University and pursuing a doctor's degree in microbiology. His main research interests are environmental microorganisms, and two papers have been published in SCI. He is also an adviser of iGEM team of Lanzhou University.
 
       </p>
 
       </p>
−
      <div class="col s12">  
+
        <p>ATTRIBUTION:</p>
−
         <div class="col s12 m6 l6">
+
        <div class="col s12">
−
          <canvas id="figure5b" height="250px"></canvas>
+
         <img style="width:50px" src="https://static.igem.org/mediawiki/2015/c/c9/Lzu_china_2015_Hardwaredesign.png">
−
          <h6 class="center-align">Figure5-B</h6>
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png">
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/1/13/Lzu_china_2015_Experiment_design.png">
 
         </div>
 
         </div>
−
        <div class="col s0 m1 l1">&nbsp;</div>
+
    </div>
−
         <div class="col s12 m5 l5">
+
  </div>
−
          <p>Fig 5-B Riboflavin concentration of  K1755005, K1755006, K1755009 bacterial strains vary with time.
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(189, 212, 253);">
−
          </p>
+
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
</div><!--L5-->
 +
          
 +
<div class="col s12" style="background-color: rgb(240, 205, 165);">
 +
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(240, 205, 165);">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Rong Xu</h4>
 +
      <p>born in July 1987, is a doctor candidate studying in Lanzhou University. She is an expert of heavy metal reduction and cellulose degradation using gene modified bacteria and she does enjoy her researches. Moreover, she helps us with her enthusiasm overcome many kinds of problems about our project. So we believe that she is one of the best advisors in our team-LZU CHINA to attend this competition.
 +
      </p>
 +
      <br/>
 +
        <p>ATTRIBUTION:</p>
 +
        <div>                   
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
 
         </div>
 
         </div>
−
      </div>
+
    </div>
 +
  </div>
 +
    <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/3/3f/LZU_China_Team_xurong.jpg" class="responsive-img avatr">
 +
  </div>
 +
 
 +
</div><!--R6-->
  
−
        
+
<div class="col s12" style="background-color: rgb(81, 212, 90);">
−
       <p>
+
  <div class="col s12 m12 l4">
−
        The bacterial strain linked with RibB has a better performance of producing riboflavin and the promoting ability from strong to weak can be easily seen as following: K1755005, K1755009 and K1755006. Moreover, the distinction between K1755009 and K1755006 isn't obvious. The construction of these three parts are familiar and the the statistical analysis also showed that when the bacterial strain linked different parts, the ability of producing riboflavin is totally different with the original BL21 (The analysis result is shown in table 2), which proved that K1755005, K1755009 and K1755006 have all accomplished as we anticipated.
+
    <img src="https://static.igem.org/mediawiki/2015/thumb/b/bd/LZU_China_Tean_duxuejing.jpg/600px-LZU_China_Tean_duxuejing.jpg" class="responsive-img avat">
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
       <h4>Xuejing Du</h4>
 +
       <p>Xuejing Du is one of the student leader of group members in our project. His interest is microbiology and behavioural sciences. It is he who has recruited the key members of this team and has brought a new concept to MFCs. He not only wrote over the 80% of the paperwork but also has done 90% of the artwork for wiki, poster and uniform. Human practice, which we thought a tough, sophisticated burden, was carried by him. In addition, he has designed a questionnaire and made a survey about people's attitude towards sewage treatment and the use of microbial fuel cells in China and UK.His creative ideas help us vastly. Now, he is preparing for the presentation in order to help our team to attend Giant Jamboree in October.
 
       </p>
 
       </p>
−
 
+
        <p>ATTRIBUTION:</p>
−
      <div class="col s12">  
+
         <div class="col s12">
−
         <div class="col s12 m6 l6">
+
            <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
−
          <canvas id="figure5c" height="250px"></canvas>
+
            <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Artdesign.png">
−
          <h6 class="center-align">Figure5-C</h6>
+
            <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
−
        </div>
+
−
        <div class="col s0 m1 l1">&nbsp;</div>
+
−
        <div class="col s12 m5 l5">
+
−
          <p>Fig 5-C Bacteria concentration of K1755007 and K1755008 bacterial strains vary with time.
+
−
          </p>
+
 
         </div>
 
         </div>
−
      </div>
+
    </div>
−
      <p>Although there are some difference in bacteria density between strain K1755007 and strain K1755008 at the same time, the difference isn't obvious. Statistical analysis also shows that the difference of bacteria density between K1755007 and control group isn't obvious, but the difference of bacteria density between K1755008 and control group is remarkable.
+
  </div>
−
          </p>
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(81, 212, 90);">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
</div><!--L7-->
  
−
      <div class="col s12">  
+
<div class="col s12">
−
        <div class="col s12 m6 l6">
+
  <div class="col s12 m12 l2 teamblock">
−
          <canvas id="figure5d" height="250px"></canvas>
+
      <h1>&nbsp;</h1>
−
          <h6 class="center-align">Figure5-D</h6>
+
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Yaxin Pei</h4>
 +
      <p>born in July 1987, is a doctor candidate studying in Lanzhou University. She is an expert of heavy metal reduction and cellulose degradation using gene modified bacteria and she does enjoy her researches. Moreover, she helps us with her enthusiasm overcome many kinds of problems about our project. So we believe that she is one of the best advisors in our team-LZU CHINA to attend this competition.
 +
      </p>
 +
      <br/>
 +
        <div>                 
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 
         </div>
 
         </div>
−
        <div class="col s0 m1 l1">&nbsp;</div>
+
    </div>
−
         <div class="col s12 m5 l5">
+
  </div>
−
        <p>Fig 5-D Riboflavin concentration of K1755007 and K1755008 bacterial strains vary with time.
+
    <div class="col s12 m12 l4">
−
</p>
+
    <img src="img/p/lzuxurong.jpg" class="responsive-img avatr">
 +
  </div>
 +
 
 +
</div><!--R8-->
 +
          
 +
<div class="col s12" style="background-color: rgb(185, 236, 237);">
 +
  <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/c/cd/Lzu_china_2015_Lzuzhangzhengdong.jpg
 +
" class="responsive-img avat">
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Zhengdong Zhang</h4>
 +
      <p>Amongst all the members struggling for our project, I rank as those busiest guys. My name is Zhengdong Zhang, working like a dog, dressed as a dull nerd. Not am I clever, but the reason why I had been busy was just I have few hobby. Like many of our team, biology science is my only lover.
 +
      </p>
 +
        <p>ATTRIBUTION:</p>
 +
        <div class="col s12">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
 
         </div>
 
         </div>
−
      </div>
+
    </div>
−
       <p>
+
  </div>
−
         After linking different parts, riboflavin expression level is distinctly different because K1755007 is constructed with strong promoter and strong RBS so that it has the strongest ability to produce riboflavin followed by K1755008, which is weaker than K1755007. The experimental phenomena coincide with theoretical analysis, showing that K1755007 and K1755008 both can work. Some problematic data have been removed.
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(185, 236, 237);">
 +
       <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
</div><!--L9-->
 +
          
 +
<div class="col s12" style="background-color: rgb(171, 170, 80);">
 +
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(171, 170, 80);">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Feifan Xu</h4>
 +
      <p>My name is Feifan Xu, a junior student. It is my great honor to become part of iGEM LZU-China 2015. To be honest, I am a diffident guy more or less. So work like electrophoresis PCR or other molecule experiment are quite suitable for me. My primary job is parts construction. Besides that, the measurement of part expression quantity and a little MFC work is also my duty. I like swim in my free time. I love computer games too. After the experiment of the passing year, I find out a truth. The only failure is give up, if you keep fighting, hope is coming.
 
       </p>
 
       </p>
−
 
+
      <br/>
−
 
+
        <p>ATTRIBUTION:</p>
−
      <div class="col s12">  
+
        <div>               
−
         <div class="col s12 m6 l6">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
−
          <canvas id="figure5e" height="250px"></canvas>
+
         <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
−
          <h6 class="center-align">Figure5-E</h6>
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/35/Lzu_china_2015_Wikicode.png">
 
         </div>
 
         </div>
−
        <div class="col s0 m1 l1">&nbsp;</div>
+
    </div>
−
         <div class="col s12 m5 l5">
+
  </div>
−
          <p>Fig 5-E The ratio of riboflavin and bacteria concentration of different kinds of BL21 vary with time.
+
    <div class="col s12 m12 l4">
−
          </p>
+
    <img src="https://static.igem.org/mediawiki/2015/1/1a/Lzu_china_2015_Lzuxufeifan.jpg
 +
" class="responsive-img avatr">
 +
  </div>
 +
 
 +
</div><!--R10-->
 +
          
 +
<div class="col s12" style="background-color: rgb(189, 193, 194);">
 +
  <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/3/3e/Lzu_china_2015_Lzudengfei.jpg
 +
" class="responsive-img avat">
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Fei Deng</h4>
 +
      <p>Fei Deng majors in biology and he seems like a serious guy. Indeed, He is strict with lots of things, such as the operations of our experiments. But he is an easygoing man in daily life. He likes a lots of activities including playing football, reading books. While he said that he cannot tell us what he loves most. In this program, he constructed some parts such as part K1755003 and examined some parts to verify that they can really work. Besides, he wrote some text for our wiki.  
 +
      </p>
 +
        <p>ATTRIBUTION:</p>
 +
        <div class="col s12">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
 
         </div>
 
         </div>
−
      </div>
+
    </div>
−
        
+
  </div>
−
       <p>
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(189, 193, 194);">
−
         Through the ratio curve of riboflavin and OD value from the same serial number and the same time point, we can intuitively know that K1755007 and K1755008 can observably improve riboflavin production in E.Coli and K1755007 behaves batter than K1755008.
+
       <h1>&nbsp;</h1>
−
 
+
       <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
</div><!--L11-->
 +
          
 +
<div class="col s12" style="background-color: rgb(147, 188, 210);">
 +
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(147, 188, 210);">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Liu Yang</h4>
 +
      <p>Her name is Liu Yang, a junior student majoring in life science. As an outgoing girl, she likes travelling in nature. With the ardent love of synthesis biology, she constructed the part and detected its work in MFC for our team this year. Although she failed a lot, she never gave up but continued working and eventually overcame all the tough things. She’s a girl of real strength.
 
       </p>
 
       </p>
−
 
+
       <br/>
−
       <p>
+
        <p>ATTRIBUTION:</p>
−
         Using SPSS (Statistical Product and Service Solutions) paired sample t test for significance test, we obtain the following results on the condition that the confidence level is 95%.
+
         <div>                 
−
Table 2 Significance test between different part and BL21
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
−
 
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png">
 +
        </div>
 +
    </div>
 +
  </div>
 +
    <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/2/20/Lzu_china_2015_Lzuyangliu.jpg
 +
" class="responsive-img avatr">
 +
  </div>
 +
 
 +
</div><!--R12-->
 +
       
 +
<div class="col s12" style="background-color: rgb(187, 36, 25);">
 +
  <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/4/45/Lzu_china_2015_Lzuyaomengna.jpg
 +
" class="responsive-img avat">
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Mengna Yao</h4>
 +
      <p>Mengna Yao is a junior student who loves life sciences so much that she ‘s always happy as long as in lab. She did some parts’ work , made booklets and assist in designing poster and wiki. She likes watching anime, being eager for anything unusual. She’s a patient and honest girl, mild and energetic.
 
       </p>
 
       </p>
−
 
+
         <p>ATTRIBUTION:</p>
−
 
+
         <div class="col s12">
−
      <div class="col s12">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
−
         <ul>
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
−
          <li class="sidelist">
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Artdesign.png">      
−
          A
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/2/25/Lzu_china_2015_Devicerunning.png">  
−
            <ul>
+
−
              <li class="sidelist">A
+
−
              <div class="bl">dada</div>
+
−
              </li>
+
−
              <li class="sidelist">B</li>
+
−
              <li class="sidelist">C</li>
+
−
            </ul>
+
−
          </li>
+
−
          <li class="sidelist">
+
−
           
+
−
          </li>
+
−
          <li class="sidelist">
+
−
           
+
−
          </li>
+
−
        </ul>
+
−
      </div>
+
−
 
+
−
      <table class="responsive-table striped">
+
−
        <thead>
+
−
          <tr>
+
−
              <th data-field="id">NO.</th>
+
−
              <th data-field="name">Bacteria concentration
+
−
</th>
+
−
              <th data-field="price">Riboflavin concentration
+
−
</th>
+
−
          </tr>
+
−
        </thead>
+
−
 
+
−
        <tbody>
+
−
          <tr>
+
−
            <td>Alvin</td>
+
−
            <td>Eclair</td>
+
−
              <td>$0.87</td>
+
−
          </tr>
+
−
          <tr>
+
−
            <td>Alan</td>
+
−
            <td>Jellybean</td>
+
−
            <td>$3.76</td>
+
−
          </tr>
+
−
          <tr>
+
−
            <td>Jonathan</td>
+
−
            <td>Lollipop</td>
+
−
            <td>$7.00</td>
+
−
          </tr>
+
−
        </tbody>
+
−
      </table>
+
−
      <h6 class="left-align">2.3 The relationship between riboflavin and produce electricity</h6>
+
−
      <p>Studies have shown that riboflavin can observably increase the voltage of MFC. By doing the experiment, we also confirmed that riboflavin can indeed improve the production capacity. And according to relevant data, we drew graph 5 (the data in this graph is measured in last year, the latest data is still in the measurement).
+
−
</p>
+
−
      <p><strong>img here!</strong></p>
+
−
      <p>Fig 6 The effects of riboflavin on voltage.
+
−
</p>
+
−
<p>
+
−
  Through the significant changes in the voltage of MFC before and after adding riboflavin, we can know that riboflavin can improve the voltage of MFC obviously.
+
−
</p>
+
−
<ul>
+
−
  <li>1.  Fischer, M. and A. Bacher, Biosynthesis of vitamin B2: Structure and mechanism of riboflavin synthase. Arch Biochem Biophys, 2008. 474(2): p. 252-65.
+
−
</li>
+
−
<li>
+
−
  2.  Pedrolli, D., et al., The ribB FMN riboswitch from Escherichia coli operates at the transcriptional and translational level and regulates riboflavin biosynthesis. FEBS J, 2015. 282(16): p. 3230-42.
+
−
</li>
+
−
<li>
+
−
  3.  Lin, J.W., Y.F. Chao, and S.F. Weng, Riboflavin synthesis genes ribE, ribB, ribH, ribA reside in the lux operon of Photobacterium leiognathi. Biochem Biophys Res Commun, 2001. 284(3): p. 587-95.
+
−
</li>
+
−
</ul>
+
−
         <h5 class="left-align">Pollutant sensor</h5>
+
−
          <p>1  Cu<sup>2+</sup> sensor(& Cr<sup>6+</sup>sensor)</p>
+
−
          <p>1.1  BBa_K1755301</p>
+
−
          <P>1.1.1  The composition of BBa_K1755301</P>
+
−
          <p>We connected BBa_K1755401（MarO）, as a promoter, with BBa_1755003（RBS+ribB CDS+B0014）to get BBa_K1755301.
+
−
</p>
+
−
          <p>1.1.2  How does it work</p>
+
−
          <p>MarO is a promoter, which belongs to the mar operon in E.coli. Genetic organization of the mar regulon in E.coli shows as Fig 2a. There are two promoter sites on the marO operator, and both of them can be bound by a kind of regulatory protein called MarR, which is coded by MarR and can repress the expression of marO(Fig 2b and Fig 2c)<sup>[2]</sup>. MarR is a very special and vital transcription factor, which can bound to either of marO’s two promoter sites rs1 and rs2(rs means repressor binding site). And MarR repressor is homodimer. And it can form tetramer once there is copper for its special structure(Fig 3). Copper(II) oxidizes a cysteine residue (Cys80) on MarR to generate disulfide bonds between two MarR dimers, thereby inducing tetramer formation and the dissociation of MarR from its cognate promoter DNA, two sites of marO(Fig 4)<sup>[1]</sup>. Some experiments firmed the explanation(Fig 5)<sup>[1]</sup>.</p>
+
−
          <p>
+
−
So we chose promoter marO as the copper sensor. Cu<sup>2+</sup> can oxidize Cys80 and make MarR apart from marO to derepress the MarR dimer. Than the promoter marO will start the following genes’ transcription. In our part BBa_K1755301, the report gene is riboflavin. So we associated the copper concentration with the output of riboflavin.
+
−
          <p>During our experiments, it is a big surprise for us to find that the marO is able to respond to Cr6+ as well though it is not so strong as Cu<sup>2+</sup>.    </p>
+
−
          <div class="col s12">
+
−
          <img src="https://static.igem.org/mediawiki/2015/f/f8/Lzu_china_2015_Ps-f2a.png
+
−
" class="responsive-img"/>
+
−
          <h6>
+
−
              Fig 2a Genetic organization of the mar operon in E.coli.</h6></div>
+
−
          <div class="col s12">
+
−
          <img src="https://static.igem.org/mediawiki/2015/3/3c/Lzu_china_2015_Ps-f2b.png
+
−
" class="responsive-img"/>
+
−
          <h6>
+
−
              Fig 2b The products of marR can repress the function of marO in E.coli</h6></div>
+
−
          <div class="col s12">
+
−
          <img src="https://static.igem.org/mediawiki/2015/4/47/Lzu_china_2015_Ps-f2c.png" class="responsive-img"/>
+
−
          <h6>
+
−
              Fig 2c The 2 sites of promoter marO in E.coli </h6></div>
+
−
          <div class="col s12">
+
−
          <img src="https://static.igem.org/mediawiki/2015/9/9d/Lzu_china_2015_Ps-f3.png
+
−
" class="responsive-img"/>
+
−
          <h6>
+
−
            Fig 3 Crystal structure of copper(II)-oxidized MarR5CS(80C).<br/>
+
−
(a) Copper(II)-oxidized MarR5CS(80C) tetramer with two monomers from each dimer (chain A–chain B and chain A’–chain B’) colored blue and magenta, respectively. Atoms in the side chains of Cys80 from all four of the monomers are shown as yellow spheres. (b) Close-up of the two disulfide bonds between two MarR dimers. The disulfide bonds shown in yellow sticks. Only residues Asp67(Asp67’), Cys80(Cys80’) and Gly82(Gly82’) from each monomer are shown for clarity. (c) Superposition of the structures of copper-oxidized MarR5CS(80C) (blue) and the previously reported SAL-complexed WT MarR(orange).
+
−
 
+
−
</h6></div>
+
−
          <div class="col s12">
+
−
          <img src="https://static.igem.org/mediawiki/2015/6/6f/Lzu_china_2015_ps-f4.png
+
−
" class="responsive-img"/>
+
−
          <h6>Fig 4 The mechanism of Cu<sup>2+</sup> derepress of MarO
+
−
 
+
−
</h6></div>
+
−
          <div class="col s12">
+
−
          <img src="https://static.igem.org/mediawiki/2015/0/06/Lzu_china_2015_Ps-f5.png
+
−
" class="responsive-img"/>
+
−
          <h6>Fig 5 Some experiments results verified the explanation of the mechanism of MarO[1]
+
−
 
+
−
         
+
−
              <h6>1.1.3 Results
+
−
      <h6>We linked BBa_K1755301 into pSB1C3 and transformed the new plasmid into E.coli BL-21. Then we detected the concentration of          riboflavin through measuring the absorbance under 444nm and found that riboflavin production basically response to the change of metal ion concentration as it present a linear relationship with Cu2+  concentration, which indicated that BBa_K1755301 can work as we anticipated.
+
−
 
+
−
 
+
−
 
+
−
</h6></div>
+
−
          <div class="col s12">
+
−
          <img src="https://static.igem.org/mediawiki/2015/2/22/Lzu_china_2015_Ps-f6.png
+
−
" class="responsive-img"/>
+
−
        </div>
+
−
          <p>1.2 BBa_K1755302</p>
+
−
          <p>1.2.1 The composition of BBa_K1755302</p>
+
−
          <p>We connected BBa_K190024（CueO+RBS）, as a, with BBa_1755002（ribB CDS+B0014）to get BBa_K1755302.  The former part is a sensor and the latter is a reporter.</p>
+
−
          <p>1.2.2 How does it work</p>
+
−
          <p>CueO can express periplasmic copper-binding proteins, CueO , a multi-copper oxidase for detoxification<sup>[1]</sup>, which belongs to CueR Regulon gene and can be regulated by copper-responsive transcription factor CueR, too. CueO can oxidate Cu+ to Cu2+ and decrease the toxicity of copper<sup>[3, 4]</sup>. On the CueO promoter, CueR protectes -41 to -17 on top strand and -42 to -19 on bottom strand to repress the expression of CueO<sup>[1]</sup>. And it can be derepressed by Cu<sup>2+</sup>, too. So the CueO promoter can sense the concentration of copper and linkage it with the production of riboflavin.
+
−
</p>
+
−
          <p>1.2.3 Results
+
−
</p>
+
−
          <p>
+
−
          We linked BBa_K1755302 into pSB1C3 and transformed the new plasmid into E.coli BL-21. Then we detected the concentration of riboflavin through measuring the absorbance under 444nm and found that riboflavin production basically response to the change of metal ion concentration as it present a linear relationship with Cu<sup>2+</sup>  concentration, which indicated that BBa_K1755302 can work as we anticipated.
+
−
</p>
+
−
          <div class="col s12">
+
−
          <img src="https://static.igem.org/mediawiki/2015/f/f3/Lzu_china_2015_Ps-f7.png
+
−
" class="responsive-img"/>
+
−
        </div>
+
−
          <p>2 Hg<sup>2+</sup> sensor</p>
+
−
          <p>2.1 BBa_K1755305</p>
+
−
          <p>2.1.1 The composition of BBa_K1755305</p>
+
−
          <p>We connected BBa_K346002（PmerT）, as a promoter, with BBa_1755003（RBS+ribB CDS+B0014）to get BBa_K1755305.
+
−
</p>
+
−
          <p>2.1.2 How does it work</p>
+
−
 
+
−
          <p> PmerT is a kind of promoter from Tn21 mercury resistance (mer) operon. When there is no Hg2+, it cannot start the transcription. Upon binding the cognate metal ions, the metallated MerR homodimer causes a realignment of the promoter so that RNA polymerase contacts the -35 and -10 sequences leading to open complex formation and transcription.
+
−
(see more details http://  parts.igem.org /Part:BBa_K346002).</p>
+
−
          <p> So PmerT promoter can be induced by Hg2+ and start the coding of ribB downstream to increase the output of riboflavin. And there is some quantity relationship about the concentration of Hg2+ and the production of riboflavin. We can calculate the the concentration of Hg2+ by our “Micro Holmes” system.</p>
+
−
 
+
−
          <p>2.1.3 Results</p>
+
−
 
+
−
          <p>We transformed BBa_K1755305 into E.coli BL-21. Then we detected the concentration of riboflavin through measuring the absorbance under 444nm. Unfortunately, we didn’t find that riboflavin production respond to the change of Hg2+ concentration, which indicated that BBa_K1755305 can not work as we anticipated.
+
−
</p>
+
−
          <p>
+
−
 
+
−
          <div class="col s12">
+
−
          <img src="https://static.igem.org/mediawiki/2015/9/9b/Lzu_china_2015_Ps-f8.png
+
−
" class="responsive-img"/>
+
−
        </div>
+
−
          <p>
+
−
          3 F- sensor
+
−
</p>
+
−
          <p>3.1 BBa_K1755024
+
−
</p>
+
−
          <p>3.1.1 The composition
+
−
</p>
+
−
          <p>We used BBa_K911003 as a promoter and connected it with BBa_1755003（RBS+ribB CDS+B0014）to get 3.1 BBa_K1755024.
+
−
</p>
+
 
          
 
          
−
          <p>3.1.2 How does it work
 
−
</p>
 
−
          <p>BBa_K911003 is a fluoride sensitive riboswitch that is highly sensitive to the F-. It is a positive regulator so F- can induce the promoter to transcript. Since the report gene is at the downstream of fluoride promoter, the riboflavin production can be influenced by the change of F- concentration. So we can use the concentration of riboflavin to determine the concentration of F- and even apply into our “Micro Holmes” system.
 
−
</p>
 
−
          <p>3.1.3 Results
 
−
</p>
 
−
          <p>We transformed BBa_K1755024 into E.coli BL-21. Then we detected the concentration of riboflavin through measuring the absorbance under 444nm. However, we didn’t find regular change relationship between riboflavin production and Hg2+ concentration. The result showed that BBa_K1755024 can not work as we anticipated.
 
−
</p>
 
−
          <!--<p>
 
−
          So PmerT promoter can be induced by Hg<sup>2+</sup> and start the coding of ribB downstream to increase the output of riboflavin. And there is some quantity relationship about the concentration of Hg2+ and the production of riboflavin. We can calculate the the concentration of Hg<sup>2+</sup> by our “Micro Holmes” system.
 
−
</p>-->
 
−
          <p><div class="col s12">
 
−
          <img src="https://static.igem.org/mediawiki/2015/8/88/Lzu_china_2015_Ps-f9.png" class="responsive-img"/>
 
−
        </div></p>
 
−
         
 
−
<ul>
 
−
<li>1. Hao, Z., et al., The multiple antibiotic resistance regulator MarR is a copper sensor in Escherichia coli. Nat Chem Biol, 2014. 10(1): p. 21-8.</li>
 
−
 
−
<li>2. R., C.X.H.Z.C.P., Protein photocrosslinking reveals dimer of dimers formation on MarR protein in Escherichia coli. 中国科学：化学, 2012. 42(2): p. 223-225.</li>
 
−
 
−
<li>3. Yamamoto, K. and A. Ishihama, Transcriptional response of Escherichia coli to external copper. Mol Microbiol, 2005. 56(1): p. 215-27.</li>
 
−
 
−
<li>4. Stoyanov, J.V., J.L. Hobman, and N.L. Brown, CueR (YbbI) of Escherichia coli is a MerR family regulator controlling expression of the copper exporter CopA. Molecular Microbiology, 2001. 39(2): p. 502-512.</li>
 
−
</ul>
 
−
 
−
 
 
−
 
−
        <h5 class="left-align">Our MFC</h5>
 
−
      <p>1.Microbial Fuel Cells (MFC) are a promising technology for electricity production from a variety of materials, such as natural organic matter, complex organic waste or renewable biomass, and can be advantageously combined with applications in waste water treatment[1]. However, MFC have not achieved commercialization yet on account of low power output, which is mainly due to the low efficiency of extracellular electron transfer without the help of redox mediators added manually into the reactor, such as neutral red, thionin[2]. The E. coli K12 was cultivated and then inoculated, and the E. coli thus obtained is referred to as ‘‘original bacteria’’ which was subjected to a fuel cell discharge process until the cell voltage was below 0.05 V. The bacteria suspension that underwent fuel cell discharge was inoculated into a fresh medium and cultivated. The resulting E. coli is referred to as ‘‘electrochemical bacteria of generation I’’, which was again subjected to fuel cell discharge until the cell voltage was below 0.05 V. Repeating the inoculation, cultivation and discharge cycles produced electrochemical bacteria of generation II, III, and so on[3]. </p>
 
−
        <img class="center-align responsive-img" src="https://static.igem.org/mediawiki/2015/f/f4/Lzu_china_2015_MFC-1.jpg
 
−
" >
 
−
        <p>The variation of the anode and cathode potentials during the discharge process of the electrochemical bacteria of generation II.[3].</p>
 
−
        <p>In this study, we constructed the genetically modified E.coli with metal ion bio-sensor coupling ribB, which expression product is a redox mediator, to monitor Cu2+ concentration in aquatic environment based on dependency of peak voltage in MFC on Cu2+ content. </p>
 
−
        <p>2.</p>
 
−
        <table class="responsive-table striped">
 
−
                    <tr>
 
−
                    <td>Part ID</td>
 
−
                    <td>Characterization</td>
 
−
                    <td>&nbsp;</td>
 
−
                    </tr>
 
−
                    <tr>
 
−
                    <td>K1755301</td>
 
−
                    <td>MarO(Cu sensor) + B0032(RBS)+ ribB + B0014(terminator)</td>
 
−
                    <td>LZU-201501</td>
 
−
                    </tr>
 
−
                    <tr>
 
−
                    <td>K1755302</td>
 
−
                    <td>K190024(Copper Promoter) + ribB + B0014(terminator)</td>
 
−
                    <td>LZU-201502</td>
 
−
                    </tr>
 
−
                    <tr>
 
−
                    <td>K1755303</td>
 
−
                    <td>K540001：cobalt-sensitive promoter + B0032(RBS) + ribB + B0014(terminator)</td>
 
−
                    <td>LZU-201503</td>
 
−
                    </tr>
 
−
                    <tr>
 
−
                    <td>K1755305</td>
 
−
                    <td>K911003(fluoride sensitive riboswitch)+ B0032(RBS) + ribB + B0014(terminator)</td>
 
−
                    <td>LZU-201504</td>
 
−
                    </tr>
 
−
                    <tr>
 
−
                    <td>K1755024</td>
 
−
                    <td>K911003(Fluoride sensitive riboswitch)+ B0032(RBS) + ribB + B0014(terminator)</td>
 
−
                    <td>LZU-201505</td>
 
−
                    </tr>
 
−
                    </tbody>
 
−
                </table>
 
−
                <br/>
 
−
                <br/>
 
−
        <p>Riboflavin, also known as Vitamin B2, is a efficient redox mediator as well as a stimulator of MFC. In our study, we constructed recombinant plasmids with several different kind of pollutant substrate bio-sensors coupling ribB. Bio-sensors includes copper sensor, cobalt sensor and fluoride sensor. Then, we transformed recombinant plasmids into E.coli BL 21. As time is limited, </p>
 
−
        <p>we have only made a deep research on genetic engineered bacteria with copper bio-senso coupling ribB which can produce riboflavin when added copper in MFC anode medium. As for parts with other ion sensor, we just made them to be the formation of Biobrick. </p>
 
−
        <p>3.</p>
 
−
        <p>In our study, we've designed a novel MFC system. For anode strain, we've cloned a copper sensor sequence coupling with ribB into E.coli BL 21. The genetically modified bacteria is able to detect Cu 2+ and produce riboflavin to boost electrical generation. As a result, MFC peak voltage can reflect Cu2+ concentration. Phosphate Buffer（PBS）with 100 mmol/L potassium ferricyanide was packed into cathode chamber. </p>
 
−
        <p>We aimed to build a quantitative monitor system of Cu2+ via measuring peak voltage, and finally found the interralationship between peak voltage and Cu2+ concentration. We concluded an empirical formula for our devices:</p>
 
−
        <img class="center-align responsive-img" src="https://static.igem.org/mediawiki/2015/1/19/Lzu_china_2015_MFC-2.jpg
 
−
" >
 
−
        <br/>
 
−
        <p>Peak voltage in MFC as genetically modified E.coli were treated with different amount of Cu2+. </p>
 
−
        <p>We can deduce the concentration of Cu2+ in water by measuring peak voltage in MFC.</p>
 
−
        <br/>
 
−
        <p>In a range of copper’s low-concentration, the peak voltage rose with the increase of concentration of Cu<sup>2</sup>+. However, because of toxic effects of heavy metal to bacterium, when the copper content in water is over a certain concentration (about 2 mg/L) the peak voltage decreased as the concentration rises.  </p>
 
−
        <p>MFC Assembly and Operation</p>
 
−
        <p>1. Medium Preparation </p>
 
−
        <p>  1.1 M9 medium (per liter contains) PH=7.2</p>
 
−
        <p>    NH4Cl                1g</p>
 
−
        <p>    Casein hydrolysate      1g</p>
 
−
        <p>    KH2PO4              1g            </p>
 
−
        <p>    Na2HPO4·12H2O  15.116g  </p>
 
−
        <p>    10ml sterilized 20% glucose and 1ml 1 mol/L MgSO4·7H20 were put after 121℃ sterilization.</p>
 
−
        <p>1.2 LB medium (per liter contains] PH=7.0</p>
 
−
      <p> NaCl                  10g</p>
 
−
      <p>Yeast Extract            5g</p>
 
−
      <p>Tryptone              10g</p>
 
−
      <p>Before 121℃ sterilization, LB medium was Aliquoted into tube (each for 5mL) and conical flask (each for 150ml), respectively used for the activation of the strain and the amplification culture.</p>
 
−
      <p>2. Preparation of phosphate buffer (per liter contains)</p>
 
−
      <p>K2HPO4•3H2O                      6.618g</p>
 
−
      <p>KH2PO4                            2.858g</p>
 
−
      <p>Potassium ferricyanide          32.925g</p>
 
−
      <p>3. Bacteria activation and amplification culture</p>
 
−
      <p>3.1 Bacteria activation</p>
 
−
      <p>Materials: tips, pipettes, sterilized tubes, test-tubes rack, LB medium (tube)</p>
 
−
      <p>Bench was cleaned by ultraviolet light vertical irradiate for 20 minutes. Then 200ul 1755301 bacterial glycerol stock  and 5ul 25mg/ml chloramphenicol were added into a tube,and was then shaked for 12 hours in a shaker at 37 degrees Celsius.</p>
 
−
      <p>3.2 Amplification culture</p>
 
−
      <p>Materials: tips, pipettes, sterilized tubes, test-tube rack, LB medium (conical flask)</p>
 
−
      <p>Bench was cleaned by ultraviolet light vertical irradiate for 20 minutes.1.5ml bacteria solution and 150ul 25mg/ml chloramphenicol were added into a conical flask of LB medium, and was then shaked for 12 hours in a shaker at 37 degrees Celsius.</p>
 
−
      <p>4. MFC Assembly</p>
 
−
      <p>4.1 Treatment of carbon fiber felt</p>
 
−
      <p>Preparation:</p>
 
−
      <p>1mol/L NaOH    1L water + 40g NaOH</p>
 
−
      <p>1mol/L HCl      1L water + 90.9mL concentrated hydrochloric acid</p>
 
−
      <p>The carbon fiber felt was first cleaned by deionized water，and was then boiled with 1mol/L NaOH and 1mol/L HCl for 30 minutes. After each boiling,it was cleaned by deionized water until its pH value is 7. Finaly it was dried at 105。C for 20 minutes.</p>
 
−
      <p>4.2 Treatment of proton exchange membrane</p>
 
−
      <p>Preparation:</p>
 
−
      <p>0.5mol/LH2SO4    1Lwater + 27.17mL concentrated hydrochloric acid</p>
 
−
      <p>The proton exchange membrane was boiled in turn in 30% H2O2, deionized water, 0.5mol/LH2SO4 and deionized water for 30 minutes.</p>
 
−
      <p>4.3Sterilization</p>
 
−
      <p>2 250ml graduated cylinders,2 250ml conical flasks,12 50ml centrifuge tubes,cathode chamber and anode chamber(with magnon),sterile water,gaskets and carbon fiber felt(fixed on titanium wire)</p>
 
−
      <p>4.4Combination of cathode chamber and anode chamber</p>
 
−
      <p>A proton exchange membrane was put between the anode and the cathode,fixed with gaskets and parafilm.Check for leaks with sterile water.</p>
 
−
      <p>4.5Treatment of bacteria solution</p>
 
−
      <p>Bacteria was cleaned with M9 medium, and the absorbance (OD values） was measured in the 600nm wavelength and were adjusted to the 1.3--1.4.</p>
 
−
      <p>4.6Add liquids to cathode chamber and anode chamber</p>
 
−
      <p>4.6.1anode chamber:</p>
 
−
      <p>24ml bacteria solution after treatment,216ml M9 medium,appropriate amount 20mg/ml CuSO4 mother solution (make sure the final concentration of Cu<sup>2</sup>+ are 0、1、2、3、4mg/L)</p>
 
−
      <p>4.6.2cathode chamber:</p>
 
−
      <p>240ml PBS（with 100mmol/L K3[Fe(CN)6)）</p>
 
−
      <p>4.7Fixed carbon fiber felt</p>
 
−
      <p>Carbon fiber felt was put in front of the carbon fiber felt,and the titanium wire tied to it was fixed on the bottle cap of cathode chamber and anode chamber.</p>
 
−
      <p>5.MFC Operation</p>
 
−
      <p>The MFC was put on the magnetic disk, the temperature and the speed of magnon were set, the temperature sensors were put into liquids，voltage was recorded every other minute.Find the relationship between peak voltage and the concentration of Cu<sup>2+</sup>.</p>
 
−
      <p>6.Determination of bacteria concentration and expression of riboflavin</p>
 
−
      <p>3ml bacteria solution was taken out from anode chamber every 12 hours(0h、12h、24h、36h、48h）, their absorbance (OD values ) were measured in the 600nm wavelength. 1ml supernatant  was added into 2mL 0.1mol/L HCl after centrifugation(12000g,2min)，their absorbance (OD values)  were measured in the 444nm wavelength,which suggects the concentration of riboflavin.</p>
 
−
      <p>1.Oliveira, V. B., Simões, M., Melo, L. F., and Pinto, A. M. F. R. (2013) Biochemical Engineering Journal 73, 53-64</p>
 
−
      <p>2.Rabaey, K., and Verstraete, W. Trends in Biotechnology 23, 291-298</p>
 
−
      <p>3.Zhang, T., Cui, C., Chen, S., Ai, X., Yang, H., Shen, P., and Peng, Z. (2006) Chemical Communications </p>
 
−
      </div>
 
−
</div>
 
−
<!------------------>
 
−
        <div class="row">
 
−
      <div id="drylab" class=" section scrollspy col s12">
 
−
        <h4 class="left-align">Dry Lab</h4>
 
−
          <div class="progress">
 
−
          <div class="determinate" style="width: 0%"></div>
 
 
         </div>
 
         </div>
−
        <h5 class="left-align">Overview</h5>
+
    </div>
−
 
+
  </div>
−
 
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(187, 36, 25);">
−
          <P>“Micro Holmes concentration measurement system” is a system containing electronic components, mechanical components, biology components (several kinds of genetic engineered bacteria) and software working with them. Using this system, you can monitor the contaminants concentrations whenever and wherever in a cheap and easy way. </P>
+
      <h1>&nbsp;</h1>
−
          <P>A typical “Micro Holmes concentration measurement system” at least include:</P>
+
      <ul>
−
          <ul>
+
      <li>OverGraduate</li>
−
              <li>A Microbial Fuel Cell<br/>
+
      <li>UnderGraudate</li>
−
                &nbsp;-- cathode chamber and anode chamber<br/>
+
      </ul>
−
                &nbsp;-- carbon fiber felt <br/>
+
  </div>
−
                &nbsp;-- proton exchange membrane</li>
+
</div><!--L13-->
−
              <li>A MCU -- such as pcDuino3 board</li>
+
−
              <li> A voltage magnification circuit board</li>
+
−
              <li>An Analogue-to-Digital Conversion chip</li>
+
−
              <li>A set of input and output devices – such as touch screen</li>
+
−
              <li>One or several kinds of bacteria – such as genetic engineered E.coli</li>
+
−
          </ul>
+
−
        <p>Till now, we have made two versions of Micro Holmes concentration measurement system, the professional version and the portable version. Their main principles are totally same. The only difference is that the professional version has a set of complicated MFCs (6 cathode and anode chambers with thermostats and magnetic stirrers) and can automatically change sample. On the other hand, the portable version has its own incomparable merits. Portable ones are cheap, handiness and fit for complex working environment.</p>
+
−
        <p>We will bring a portable version of our “Micro Holmes concentration measurement system” to the Giant Jamboree. So this Manual will concentrate on the portable version.</p>
+
−
 
+
−
        <h5>Quick Guide</h5>
+
−
 
+
−
     
+
−
        <P>1.  Power On: Press the power button more than 3 seconds till the power light sparkles.</P>
+
−
        <P>2.  The touch screen will show the “Welcome screen”, please wait until the “MODE” screen appears.</P>
+
−
        <P>3.  Select the “Standard Cure (Initialization)” mode to work out a formula showing the correlation between the concentration of contaminants and the output voltage of MFC (or load an existing one)</P>
+
 
          
 
          
−
        <img src="https://static.igem.org/mediawiki/2015/5/5a/Lzu_china_2015_Image001.jpg
+
<div class="col s12" style="background-color: rgb(201, 201, 199);">
−
" class="responsive-img">
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(201, 201, 199);">
−
        <p>4.  If you want to load an existing formula, just click the “OPEN” icon, and then select a save file (Then please go to step 7). If you want to work out a new formula please click “ADD”, and then input the current concentration of contaminants ended with the button “OK”.</p>
+
      <h1>&nbsp;</h1>
−
        <img src="https://static.igem.org/mediawiki/2015/a/ac/Lzu_china_2015_Image003.png
+
      <ul>
−
" class="responsive-img">
+
      <li>OverGraduate</li>
−
        <p>5. The system will begin to real-time monitor the output voltage of MFC within a period of time until the system thinks it has gotten the peak voltage value. And then the concentration of the contaminants and the peak output voltage of the MFC will appear in the “History” list.</p>
+
      <li>UnderGraudate</li>
−
        <img src="https://static.igem.org/mediawiki/2015/9/9e/Lzu_china_2015_Image005.png
+
      </ul>
−
" class="responsive-img">
+
  </div>
−
        <p>6. Change the standard sample manually (portable version user) or automatically (professional version user), click “ADD”, and then input the new concentration of current contaminants ended with the button “OK”. Repeat step 5 and step 6 until all the standard samples have been measured. Then click “Finish” to calculate the fitting formula.</p>
+
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Yu Chen</h4>
 +
      <p>Yu Chen, undergraduate student of biology in Lanzhou University, has a great enthusiasm in doing experiment. As a member of the 2015 iGEM team of Lanzhou University, he devotes himself into making parts and MFC. He likes reading, film and animation, he also likes to play table tennis with his friends in his free time.
  
−
        <p>7. Change the sample (to be tested) manually (portable version user) or automatically (professional version user). And then select the "Real-time Monitoring" mode.</p>
+
      </p>
−
 
+
      <br/>
−
        <p>8. In this mode, you can watch the real-time output voltage and the concentration of the sample calculated using the formula gotten in the “Initialization” mode. In the right zone of the screen, you can also get the historical information and observe the variation trend of the sample concentration.
+
        <p>ATTRIBUTION:</p>
−
</p>
+
        <div>                  
−
<p>1. We offer “micro Holmes cloud services for all the system users. So when your device is in the "Real-time Monitoring" mode, you can acquire the real-time data through the Internet any place any time.
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
−
</p>
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
−
<p>1. You can also use a mobile phone with Bluetooth to control your “Micro Holmes concentration measurement system” in a convenient way.
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
−
</p>
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/35/Lzu_china_2015_Wikicode.png">
−
<img src="https://static.igem.org/mediawiki/2015/d/d6/Lzu_china_2015_Image007.png
+
−
" class="responsive-img">
+
−
<h5>Technical Details (Instruction of mv Analysis)</h5>
+
−
<p>About our device, I want to state from two part.</p>
+
−
 
+
−
<h6>Hardware overview
+
−
</h6>
+
−
 
+
−
<p>Our device’s hardware is component of three device, the first is  MCU—pcDuino3, pcDuino3 is a high performance, cost effective single board computer. It runs operation systems such as Ubuntu Linux and Android. pcDuino3 has HDMI interface to output its graphic desktop screen. It could support multi-format 1080p 60fps video decoder and 1080p 30fps H.264 and MPEG4 video encoder with its built-in hardware video processing engine. It targets specially the fast growing demands from the open source community. pcDuino3 provides easy-to-use tool chains and is compatible with the popular Arduino ecosystem such as Arduino Shields.</p>
+
−
<p>Features & Highlights:
+
−
</p>
+
−
 
+
−
<ul>
+
−
<li>• 100% compatible with original Arduino Shields</li>
+
−
<li>• 100% compatible with Linux and Android</li>
+
−
<li>• Further support for:</li>
+
−
<li>• C, C ++ with GNU tool</li>
+
−
<li>• Java with standard Android SDK Python</li>
+
−
<li>• Arduino pin header, Aduino UNO Slots: 14x GPIO, 2x PW M, 6x ADC, 1x UART, 1xSPI, 1x I2C</li>
+
−
<li>• Ethernet 10M/100Mbps, WiFi, SATA</li>
+
−
</ul>
+
−
<img src="https://static.igem.org/mediawiki/2015/d/d0/Lzu_china_2015_Image009.png
+
−
" class="responsive-img">
+
−
<p>This board has very sufficient GPIO pins, it can make everything you think out, so we take it.</p>
+
−
<p>
+
−
And the next is the another important device, named voltage magnification, since the voltage from microorganism reaction is very low, so if we want to read the value precise, before reading this value, we must amplify it, and then connect it to my MCU’s simulation pins, and by the helping of A-D chip, we can make this simulate value to digital value, then, the MCU can process this value, what out’s doing is display this value to screen—this screen is a touchable screen, so the input to the MCU, we can make a software to access.
+
−
</p>
+
−
<img src="https://static.igem.org/mediawiki/2015/4/42/Lzu_china_2015_Image011.png
+
−
" class="responsive-img">
+
−
<p>the connection of screen and pcduino
+
−
</p>
+
−
<img src="https://static.igem.org/mediawiki/2015/0/02/Lzu_china_2015_Image013.png
+
−
" class="responsive-img">
+
−
 
+
−
<h6>Software Overview
+
−
</h6>
+
−
 
+
−
<p>We design this software to read and process the voltage value.
+
−
The design tool kit includes three part, the first is demo, since some all knows reasons, we cannot take microorganism outside, so, we have to make a module named demo, from the demo part, you can know, how this hardware and software work. The second module is analysis part, the hardware real working, reading the voltage value from the microorganism reaction, and processed by the formula—we get this formula also by this MCU process—and then get the value of density of oil plants. The third part is pre-calculation part, this part</p>
+
−
 
+
−
 
+
−
      </div>
+
 
         </div>
 
         </div>
−
        <div id="parts" class="section scrollspy row">
 
−
      <div id="popout" class="col s12">
 
−
        <h4 class="left-align">Part</h4>
 
−
          <div class="progress">
 
−
          <div class="determinate" style="width: 0%"></div>
 
−
        </div>
 
−
        <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
          <h5 class="left-align">Part list</h5>
 
−
 
−
      </div>
 
 
     </div>
 
     </div>
 +
  </div>
 +
    <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Lzuchenyu.jpg" class="responsive-img avatr">
 +
  </div>
 +
 
 +
</div><!--R14-->
 +
       
 +
<div class="col s12" style="background-color: rgb(247, 214, 176);">
 +
  <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/a/af/Lzu_china_2015_Lzuyangmingchu.jpg
 +
" class="responsive-img avat">
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Mingchu Yang</h4>
 +
      <p>My name is Mingchu Yang. In China “YANGMING” mean “famous”. And if you read my name upside down “CHUMING” mean famous too. So my dad gave me the name of a certain hope I can have a wonderful future. This time in Igem, I gain a lot. Not only increased my knowledge also strengthened the ability experiment. Above all, in this team, I got the most important thing. That is friends.
  
−
       <!--<div id="popout" class="col s12">
+
       </p>
−
        <h4 class="left-align">Part</h4>
+
        <p>ATTRIBUTION:</p>
−
           <div class="progress">
+
        <div class="col s12">
−
           <div class="determinate" style="width: 0%"></div>
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Artdesign.png">
 +
           <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
 +
           <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 
         </div>
 
         </div>
−
        <h5 class="left-align">Part list</h5>
+
    </div>
−
</div>-->
+
  </div>
 +
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(247, 214, 176);">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
</div><!--L15-->
 +
       
 +
<div class="col s12" style="background-color: rgb(187, 119, 96);">
 +
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(187, 119, 96);">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Huzi Cheng</h4>
 +
      <p>Huzi Cheng designed both the visual looks and logical struct of our wiki. He also in charge of  building, uploading and making sure it can works well and being attractive to viewers.He designed our annual logo ,also.He joined the visualization and the fitting of our data.
  
−
<div id="interlab" class="section scrollspy row">
+
      </p>
−
       <div id="popout" class="col s12">
+
       <br/>
−
        <h4 class="left-align">Interlab</h4>
+
      <p>ATTRIBUTION:</p>
−
          <div class="progress">
+
        <div>                
−
          <div class="determinate" style="width: 0%"></div>
+
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Artdesign.png">
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/35/Lzu_china_2015_Wikicode.png">
 
         </div>
 
         </div>
−
        <h5 class="left-align">to be finished</h5>
+
    </div>
−
          <h5 class="left-align">to be finished</h5>
+
  </div>
−
          <h5 class="left-align">to be finished</h5>
+
    <div class="col s12 m12 l4">
−
          <h5 class="left-align">to be finished</h5>
+
    <img src="https://static.igem.org/mediawiki/2015/thumb/2/2a/LZUChenghuzi.jpg/576px-LZUChenghuzi.jpg" class="responsive-img avatr">
−
          <h5 class="left-align">to be finished</h5>
+
  </div>
−
          <h5 class="left-align">to be finished</h5>
+
 
−
          <h5 class="left-align">to be finished</h5>
+
</div><!--R16-->
−
          <h5 class="left-align">to be finished</h5>
+
       
−
          <h5 class="left-align">to be finished</h5>
+
<div class="col s12" style="background-color: rgb(238, 238, 223);">
−
          <h5 class="left-align">to be finished</h5>
+
  <div class="col s12 m12 l4">
−
          <h5 class="left-align">to be finished</h5>
+
    <img src="https://static.igem.org/mediawiki/2015/1/1e/Lzu_china_2015_Lzushaotianyi.jpg
−
          <h5 class="left-align">to be finished</h5>
+
" class="responsive-img avat">
−
          <h5 class="left-align">to be finished</h5>
+
  </div>
−
       </div>
+
  <div class="col s12 m12 l6">
−
</div>
+
    <div class="row">
−
 
+
      <h4>Tianyi Shao</h4>
−
<div id="future" class="section scrollspy row">
+
      <p>Hello,I am Tianyi Shao majoring in Chemistry from Lanzhou University.I don’t mean to exaggerate how splendidly our project performs in that Micro-Holmes as a pollutant detector is pragmatic and useful in every corner of this contaminated world.Three other partners and I are primarily in charge of the construction and operation of the MFCs,and collecting the data for an experienced formula that has widely applicability.I went to Japan for communication with top Japanese iGEM’s undergraduates in this summer and we all enjoyed this kind of opportunities to share our precious ideas.I am looking forward to having a brainstorm with you coming from all over the world in Boston.
−
      <div id="popout" class="col s12">
+
       </p>
−
        <h4 class="left-align">Future</h4>
+
        <p>ATTRIBUTION:</p>
−
          <div class="progress">
+
        <div class="col s12">
−
           <div class="determinate" style="width: 0%"></div>
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png">
 +
           <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 
         </div>
 
         </div>
−
        <h5 class="left-align">to be finished</h5>
+
    </div>
−
       </div>
+
  </div>
−
</div>
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(238, 238, 223);">
 +
      <h1>&nbsp;</h1>
 +
       <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
</div><!--L17-->
 +
       
 +
<div class="col s12" style="background-color: rgb(115, 151, 213);">
 +
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(115, 151, 213);">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Puxin Wang</h4>
 +
      <p>My name is Puxin Wang, an undergraduate student majoring in life science. As a new student in university, I couldn’t do some professional experiment but I can help them translate the experimental report into English. I also do the presentation at the same time.
  
  
 +
      </p>
 +
      <br/>
 +
          <p>ATTRIBUTION:</p>
 +
          <div>               
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
 +
          </div>
 +
    </div>
 +
  </div>
 +
    <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/4/4a/Lzu_china_2015_Lzuwangpuxin.jpg
 +
" class="responsive-img avatr">
 +
  </div>
 +
 
 +
</div><!--R18-->
 +
       
 +
<div class="col s12" style="background-color: rgb(128, 168, 77);">
 +
  <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/c/c3/Lzu_china_2015_Lzuxushuhui.png
 +
" class="responsive-img avat">
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Shuhui Xu</h4>
 +
      <p>It’s me. I’m Shuhui Xu. Love the sunshine and sunny days,and I love the nature. I am one of the guys who operate the MFC devices. Thinking about the tiny creature which creates visual voltage, exciting isn’t it ? I always believe that microorganism has a great potential. So ,let’s make it come true, step by step.
  
−
 
+
       </p>
−
 
+
        <p>ATTRIBUTION:</p>
−
       <div id="test2" class="col s12">
+
        <div class="col s12">
−
 
+
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
−
      <!--chart-->
+
           <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png">
−
      <div class="col s12">
+
           <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
−
        <div class="col s6">
+
−
           <canvas id="canvas" height="250px"></canvas>
+
−
           <h6 class="center-align">Figure1</h6>
+
 
         </div>
 
         </div>
−
         <div class="col s6">
+
    </div>
−
          <canvas id="canvas2" height="250px"></canvas>
+
  </div>
−
          <h6 class="center-align">Figure1</h6>
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(128, 168, 77);">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
</div><!--L19-->
 +
          
 +
<div class="col s12"style="background-color: rgb(44, 138, 239);">
 +
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(44, 138, 239);">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Jinjin Tong</h4>
 +
      <p>Hi, I am Jinjin Tong, an optimistic, active and sunny girl. I am a junior student majoring in life science in LanZhou University. In our team, my main task is to operate the Microbial Fuel Cells(MFC), collect data, and analyze data. I've grown a lot during preparing for the iGEM. After this summer vacation, it's going to be my last year of staying in LanZhou University. I hope I will enjoy every minute of every day with my friends and partners.
 +
      </p>
 +
      <br/>
 +
        <p>ATTRIBUTION:</p>
 +
        <div>             
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/30/Lzu_china_2015_Partexamination.png">
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png">
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">    
 
         </div>
 
         </div>
−
        <div class="col s1">
+
    </div>
−
          <p>&nbsp;</p>
+
  </div>
−
        </div>
+
    <div class="col s12 m12 l4">
−
        <div class="col s5">
+
    <img src="https://static.igem.org/mediawiki/2015/f/f3/Lzu_china_2015_Lzutongjingjing.png
−
          <p> : China, as the world largest developing country, suffers serious pollution in 21 century. Lanzhou, a capital city in northwest of China, bears serious contaminations.  
+
" class="responsive-img avatr">
 +
  </div>
 +
 
 +
</div><!--R20-->
 +
   
 +
<div class="col s12" style="background-color: rgb(159, 115, 76);">
 +
  <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/a/a9/Lzu_china_2015_Lzuyaoningning.jpg
 +
" class="responsive-img avat">
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Ningning Yao</h4>
 +
      <p>I’m Ningning Yao, major in biology from Lanzhou University Cuiying Honors College. It’s my great honor to take part in iGEM as I am enthusiasm in life science. In the past half year, I made porin, constructed k1755003, joined the communication with some universities in Japan and accomplished the previous work of interlab. I also borrowed some experimental material from Jilin University, Nanjing University and Huazhong Agricultural University. Thanks to iGEM, this half year of experience made me feel fulfillment although my work isn’t too much. Wish LZU-2015 can get a good achievement.
 
       </p>
 
       </p>
 +
        <p>ATTRIBUTION:</p>
 +
        <div class="col s12">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/93/Lzu_china_2015_MFC_experiment.png">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
 
         </div>
 
         </div>
−
       </div>
+
    </div>
−
 
+
  </div>
 +
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(159, 115, 76);">
 +
       <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
</div><!--L21-->
 +
       
 +
<div class="col s12" style="background-color: rgb(179, 193, 196);">
 +
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(179, 193, 196);">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Xinyu Zhou</h4>
 +
      <p>I'm Xinyu Zhou. An outgoing boy who loves anime very much major in biology in Lanzhou University. I like iGEM 'cause it's very creative and we can introduce our new ideas in it. This year in our team I was building part with my partners, also I took part in the human practice and what's more, I'm the lecturer of LZU in the meet up with HokkaidoU, KAIT as well as TokoyoTech in Japan.
  
−
    <p> China, as the world largest developing country, suffers serious pollution in 21 century. Lanzhou, a capital city in northwest of China, bears serious contaminations.
 
−
    </p>
 
−
    <p> China, as the world largest developing country, suffers serious pollution in 21 century. Lanzhou, a capital city in northwest of China, bears serious contaminations.
 
−
    </p>
 
  
 +
      </p>
 +
      <br/>
 +
      <p>ATTRIBUTION:</p>
 +
      <div>               
 +
      <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 +
      <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">         
 
       </div>
 
       </div>
−
         <div id="holder">
+
    </div>
 +
  </div>
 +
    <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Lzuzhouxinyu.jpg
 +
" class="responsive-img avatr">
 +
  </div>
 +
 
 +
</div><!--R22-->
 +
          
 +
<div class="col s12" style="background-color: rgb(216, 193, 221);">
 +
  <div class="col s12 m12 l4" >
 +
    <img src="https://static.igem.org/mediawiki/2015/2/21/Lzu_china_2015_Lzuyangyalan.jpg
 +
" class="responsive-img avat">
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Yalan Yang</h4>
 +
      <p>I’m Yalan Yang, originally working at the wet lab, but now somehow working for human practice. I took part in the publicity, including organizing public activity, registering Twitter and Weibo and finally, dramatizing for a small video. That’s all my work this year.
 +
 
 +
      </p>
 +
        <p>ATTRIBUTION:</p>
 +
        <div class="col s12">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 +
          <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
 
         </div>
 
         </div>
 +
    </div>
 +
  </div>
 +
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(216, 193, 221);">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
</div><!--L23-->
 +
       
 +
<div class="col s12" style="background-color: rgb(164, 174, 173);">
 +
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(164, 174, 173);" >
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Ning Chai</h4>
 +
      <p>I’m Ning Chai, a girl loves nature and is keen on plants. During the time after joining in iGEM, I spared every free time in lab. However, when I have some break time in the lab, I often listen to the pop music or read some English magazine. By the way, Durarara was watched after setting the PCR of RibB in the break time. Now, you must know some of me.
  
  
−
 
+
      </p>
 +
      <br/>
 +
      <p>ATTRIBUTION:</p>
 +
      <div>               
 +
      <img style="width:50px" src="https://static.igem.org/mediawiki/2015/0/09/Lzu_china_2015_Part_construction.png">
 +
      <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 +
      <img style="width:50px" src="https://static.igem.org/mediawiki/2015/9/99/Lzu_china_2015_Paperwork.png">
 +
      </div>
 
     </div>
 
     </div>
−
     <!------------------>    
+
  </div>
−
 
+
     <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/b/be/Lzu_china_2015_Lzuchaining.jpg
 +
" class="responsive-img right-align avatr">
 +
  </div>
 +
 
 +
</div><!--R24-->
 +
       
 +
<div class="col s12">
 +
  <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/2/21/Lzu_china_2015_Lzuyangyalan.jpg
 +
" class="responsive-img avat">
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Qiang Ao</h4>
 +
      <p>I’m Yalan Yang, originally working at the wet lab, but now somehow working for human practice. I took part in the publicity, including organizing public activity, registering Twitter and Weibo and finally, dramatizing for a small video. That’s all my work this year.
  
 +
      </p>
 +
        <p>ATTRIBUTION:</p>
 +
        <div class="col s12">
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/b/b5/Lzu_china_2015_Humanpractice.png">
 +
        <img style="width:50px" src="https://static.igem.org/mediawiki/2015/3/35/Lzu_china_2015_Wikicode.png">
 +
        </div>
 
     </div>
 
     </div>
 +
  </div>
 +
  <div class="col s12 m12 l2 teamblock">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
</div><!--L23-->
 +
</div>
  
 +
     
 +
    <div id="advis" class="col s12">
 +
    <div class="col s12" style="background-color: rgb(212, 213, 217);">
 +
  <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/5/51/LZU_China_Notes_LZUanlizhe.jpg
 +
" class="responsive-img avat">
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Lizhe An</h4>
 +
      <p>Prof. Lizhe An, professor of Life Science College, Vice president of Lanzhou University, went to Chinese Academy of Sciences and Bar-Ilan University to gain his postdoctor. Now his main research direction is botany ecology.
  
−
  <!--Side Nav-->
+
      </p>
−
  <div class="col hide-on-small-only m3 l3">
+
−
    <div class="pinnedx inorder">
+
−
    <!--<h4 class="left-align">Contents </h4>-->
+
  
−
     <ul class="sidelist section table-of-contents">
+
     </div>
−
        <li class="sidelist"><h5><a href="#background">Background</a></h5>
+
  </div>
−
       <ul class="sidelist">
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(212, 213, 217);">
−
        <li class="sidelist">Water pollution</li>
+
      <h1>&nbsp;</h1>
−
        <li class="sidelist">MFC</li>
+
       <ul>
−
        <li class="sidelist">Riboflavin</li>
+
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 
       </ul>
 
       </ul>
−
      </li>
+
  </div>
−
         <li class="sidelist"><h5><a href="#wetlab">Wet Lab</a> </h5>
+
</div><!--L1-->
−
      <ul class="sidelist">
+
          
−
       <li class="sidelist">Introduction</li>
+
    <div class="col s12" style="background-color: rgb(159, 186, 229);">
−
       <li class="sidelist">Aefficient riboflavin synthesis gene:RibB</li> 
+
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(159, 186, 229);">
−
       <li class="sidelist">Pollutant sensor</li>
+
       <h1>&nbsp;</h1>
−
       <li class="sidelist">Our MFC</li>
+
       <ul>
 +
       <li>OverGraduate</li>
 +
       <li>UnderGraudate</li>
 
       </ul>
 
       </ul>
−
       </li>
+
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
       <h4>Huyuan Feng</h4>
 +
      <p>Vice dean Prof Huyuan Feng：Prof. Huyuan Feng, Doctor of philosophy graduated in Lanzhou University, as the Vice Dean of School of Life Sciences, Lanzhou University, China will lead this team.  His present research subject is microbio strains living on Dunhuang Mogao Grotto and he devotes all his enthusiam to the study of protection of the historic treasure of the world - Dunhuang Frescoes. He is the ruler of us for the reasons that he is not only a professional expert of our topic but also an intelligent guide of all team.
  
−
        <li class="sidelist"><h5><a href="#drylab">Dry Lab</a></h5>  
+
      </p>
−
      <ul class="sidelist">
+
    </div>
−
        <li class="sidelist">Overview</li>
+
  </div>
−
        <li class="sidelist">Quick Guide</li>
+
    <div class="col s12 m12 l4">
−
         <li class="sidelist">Technical Details</li>
+
    <img src="https://static.igem.org/mediawiki/2015/9/9a/LZU_China_Team_fenghuyuan.jpg
 +
" class="responsive-img avatr">
 +
  </div>
 +
 
 +
</div><!--R2-->
 +
   
 +
    <div class="col s12" style="background-color: rgb(139, 160, 179);">
 +
  <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/2/27/LZU_China_Notes_LZUyangjinbo.jpg" class="responsive-img avat">
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Jinbo Yang</h4>
 +
      <p>Prof. Jinbo Yang, Doctor of philosophy graduated in Shanghai Research Institute of Biochemistry is also a member of Cuiying Scholar. He once worked as Postdoctoral Research Fellow, Research Associate Staff, Adjunct Staff and finally became a Consultant Staff in Lerner Research Institute, The Cleveland Clinic Foundation since 2001. He is an expert of tumor treatment and synthetic biology, and in 2013 he was the adviser of LZU-China in iGEM contest.
 +
      </p>
 +
          
 +
    </div>
 +
  </div>
 +
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(139, 160, 179);">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 
       </ul>
 
       </ul>
−
      </li>
+
  </div>
−
      <li class="sidelist"><h5><a href="#parts">Parts</a></h5>
+
</div><!--L3-->
−
       <ul class="sidelist">
+
       
−
        <li class="sidelist">Part list</li>
+
    <div class="col s12" style="background-color: rgb(110, 141, 188);">
 +
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(110, 141, 188);">
 +
      <h1>&nbsp;</h1>
 +
       <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 
       </ul>
 
       </ul>
−
      </li>
+
  </div>
−
      <li class="sidelist"><h5><a href="#interlab">Interlab</a></h5>  </li>
+
  <div class="col s12 m12 l6">
−
       <li class="sidelist"><h5><a href="#future">Future</a></h5></li>
+
    <div class="row">
−
    </ul>
+
      <h4>Xiangkai Li</h4>
 +
       <p>Prof. Xiangkai Li: Prof. Xiangka Li, PhD of microbiology in the University of Oklahoma, is also a member of Cuiying Scholar. He worked as a postdoctor fellow in Baylor College of Medicine in America when finishing his PhD. And after that, he has been a professor in microbiology and doctoral advisor in Lanzhou University till now.
 +
      </p>
 
     </div>
 
     </div>
 
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   </div>
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+
    <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/b/b0/LZU_China_Team_lixiangkai.jpg" class="responsive-img avatr">
 +
  </div>
 +
 
 +
</div><!--R4-->
 +
       
 +
    <div class="col s12" style="background-color: rgb(191, 233, 255);">
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 +
    <img src="https://static.igem.org/mediawiki/2015/d/d8/LZU_China_Team_qiuqiang.jpg" class="responsive-img avat">
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Qiang Qiu</h4>
 +
      <p>Prof. Qiang Qiu, born in may 1982，Weinan ShannXi, he gained Bachelor Degree and Doctor Degree in Life Science College of Lanzhou University in 2004 and 2011. Up to now, he has worked in State Key Laboratory of Grassland Agro-Ecosystem, College of Life Science.
 +
      </p>
 +
       
 +
    </div>
 +
  </div>
 +
  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(191, 233, 255);">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
</div><!--L5-->
 +
       
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  <div class="col s12 m12 l2 teamblock" style="background-color: rgb(242, 235, 219);">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Yongxing He</h4>
 +
      <p>Prof. Yongxing He, bachelor of life science and doctor of molecular and biochemistry in University of Science and Technology of China, went to university of California ,Berkeley in 2011 for his postdoctor fellow in biophysics. When finishing postdoctor, he has been a professor in Lanzhou University till now.
 +
      </p>
 +
    </div>
 +
  </div>
 +
    <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/9/9b/LZU_China_Team_heyongxing.jpg
 +
" class="responsive-img avatr">
 +
  </div>
 +
 
 +
</div><!--R6-->
 +
       
 +
    <div class="col s12">
 +
  <div class="col s12 m12 l4">
 +
    <img src="https://static.igem.org/mediawiki/2015/c/c5/Lzu_china_2015_Lzuzhangxiao.JPG
 +
" class="responsive-img avat">
 +
  </div>
 +
  <div class="col s12 m12 l6">
 +
    <div class="row">
 +
      <h4>Zhenmin Ling</h4>
 +
      <p>Prof. Qiang Qiu, born in may 1982，Weinan ShannXi, he gained Bachelor Degree and Doctor Degree in Life Science College of Lanzhou University in 2004 and 2011. Up to now, he has worked in State Key Laboratory of Grassland Agro-Ecosystem, College of Life Science.
 +
      </p>
 +
       
 +
    </div>
 +
  </div>
 +
  <div class="col s12 m12 l2 teamblock">
 +
      <h1>&nbsp;</h1>
 +
      <ul>
 +
      <li>OverGraduate</li>
 +
      <li>UnderGraudate</li>
 +
      </ul>
 +
  </div>
 +
</div><!--L5-->
 +
       
 +
    </div>
 +
    <div id="acknow" class="col s12">
 +
      <div class="container">
 +
          <div class="row">
 +
        <h5 class="center-align">ACKNOWLEDGEMENTS</h5>
 +
          <p class="center-align"> This project is supported by School of Life Science and Cuiying Honors College, Lanzhou University, MOST international cooperation funds, 2014DFA91340, Gansu provincial international cooperation funds, 1304WCGA176, Majorbio Cooperation and Takara China.</p>
 +
        </div>
 +
        <h6 class="center-align">Lanzhou University</h6>
 +
        <p class="center-align">Lanzhou University has been the best university in the northwestern region of China, ranking as one of the top 30 among over 1,500 universities across China. It is one of the key universities administered directly by the Ministry of Education.</p>
 +
        <h6 class="center-align">School of Life Sciences</h6>
 +
        <p class="center-align">The School of Life Sciences at Lanzhou University has a long history. It originated as the Department of Botany and the Department of Zoology of National Lanzhou University, which were founded in 1946 as the first departments of life sciences in western China. After several generations' efforts in over sixty years, the School of Life Science is in a good development trend in many aspects, such as faculty and staff, discipline construction, teaching and researching, and local service. Basing its scientific research on the cold and arid environment in western China, the faculty has made great achievements. The School of Life Sciences is active in international collaboration in order to create an open and supportive academic atmosphere, extend academic horizons, and improve the faculty and students' ability in research and creativity.</p>
 +
        <h6 class="center-align">Cuiying Honors College</h6>
 +
        <p class="center-align">Cuiying Honors College (CHC) was founded in August 2010 within Lanzhou University as part of China's Top-Notch Undergraduate Training Program. The Ministry of Education and the Ministry of Finance are investing in this new pilot scheme in order to develop future leaders with exceptional talent in the core disciplines. Every year this program only admits the best 1000 students from the 6 million new undergraduates nationwide. Lanzhou University has been selected as one of 19 institutions implementing this national program.</p>
 +
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           <h5 class="white-text">Acknowledgments</h5>
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           <h5 class="white-text">Sponsors</h5>
 
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             <li><a class="white-text" href="http://en.lzu.edu.cn/">Educational Administration Office, LZU</a></li>
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Latest revision as of 14:22, 16 September 2015

Team:LZU 2015



Our Team

A Novel Microbial Fuel Cell System that meaxure the content of estrogen


Unsplashed background img 2
icons meaning
Hardware Design
Software Design
Art Design
MFC experiment
Device running
Experiment Design
HumanPractice
PaperWork
Part construction
Part Examination
Team management
Wiki Code

Xiao Zhang

Xiao Zhang majoring in bioinformatics and microbiology is a master degree candidate studying in State Key Laboratory of Grassland Agro-ecosystem, Lanzhou University. As the captain of our team and the co-inventor of our novel device, he conceived and designed the entire MFC system. His versatility has great value for us to finish our project. He is really keen on science research, especially interested in functional genomics. So far, he has taken part in several research topics, and their scientific achievements were reported in 3 different SCI papers.

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Qi Wu

Chariness patience and happiness, she is Qi wu, a second times iGEMer. As co-captain this year, She is in charge of logistics and finance. This is a totally different experience from last year and she learned a lot.


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Haotian Wang

Haotian Wang is a junior student majoring in bioscience. He is a fan of Liverpool F. C. Being a good striker used to be his dream while he is now a sideback. He is so interested in synthetic biology that he always reads some articles before watching games. He constructed some of our parts.

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Xuguo Wang

Xuguo,Wang.As people say, guy who learning computer always a geek, yes, he is. He is a graduate student of Lanzhou University, he is learning linux kernel, besides this, he likes making some interesting electronical device, and like Quadcopter, like transform the Raspberry Pi to control his home's something. Well, that is very interesting. He is dreaming to contribute the linux kernel code, and working for this. He is a keen on computer algorithm, and hope someday, can make his own algorithm.


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Zhengjun Chen

(1985- ), male, is studying at Lanzhou University and pursuing a doctor's degree in microbiology. His main research interests are environmental microorganisms, and two papers have been published in SCI. He is also an adviser of iGEM team of Lanzhou University.

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Rong Xu

born in July 1987, is a doctor candidate studying in Lanzhou University. She is an expert of heavy metal reduction and cellulose degradation using gene modified bacteria and she does enjoy her researches. Moreover, she helps us with her enthusiasm overcome many kinds of problems about our project. So we believe that she is one of the best advisors in our team-LZU CHINA to attend this competition.


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Xuejing Du

Xuejing Du is one of the student leader of group members in our project. His interest is microbiology and behavioural sciences. It is he who has recruited the key members of this team and has brought a new concept to MFCs. He not only wrote over the 80% of the paperwork but also has done 90% of the artwork for wiki, poster and uniform. Human practice, which we thought a tough, sophisticated burden, was carried by him. In addition, he has designed a questionnaire and made a survey about people's attitude towards sewage treatment and the use of microbial fuel cells in China and UK.His creative ideas help us vastly. Now, he is preparing for the presentation in order to help our team to attend Giant Jamboree in October.

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Yaxin Pei

born in July 1987, is a doctor candidate studying in Lanzhou University. She is an expert of heavy metal reduction and cellulose degradation using gene modified bacteria and she does enjoy her researches. Moreover, she helps us with her enthusiasm overcome many kinds of problems about our project. So we believe that she is one of the best advisors in our team-LZU CHINA to attend this competition.


Zhengdong Zhang

Amongst all the members struggling for our project, I rank as those busiest guys. My name is Zhengdong Zhang, working like a dog, dressed as a dull nerd. Not am I clever, but the reason why I had been busy was just I have few hobby. Like many of our team, biology science is my only lover.

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Feifan Xu

My name is Feifan Xu, a junior student. It is my great honor to become part of iGEM LZU-China 2015. To be honest, I am a diffident guy more or less. So work like electrophoresis PCR or other molecule experiment are quite suitable for me. My primary job is parts construction. Besides that, the measurement of part expression quantity and a little MFC work is also my duty. I like swim in my free time. I love computer games too. After the experiment of the passing year, I find out a truth. The only failure is give up, if you keep fighting, hope is coming.


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Fei Deng

Fei Deng majors in biology and he seems like a serious guy. Indeed, He is strict with lots of things, such as the operations of our experiments. But he is an easygoing man in daily life. He likes a lots of activities including playing football, reading books. While he said that he cannot tell us what he loves most. In this program, he constructed some parts such as part K1755003 and examined some parts to verify that they can really work. Besides, he wrote some text for our wiki.

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Liu Yang

Her name is Liu Yang, a junior student majoring in life science. As an outgoing girl, she likes travelling in nature. With the ardent love of synthesis biology, she constructed the part and detected its work in MFC for our team this year. Although she failed a lot, she never gave up but continued working and eventually overcame all the tough things. She’s a girl of real strength.


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Mengna Yao

Mengna Yao is a junior student who loves life sciences so much that she ‘s always happy as long as in lab. She did some parts’ work , made booklets and assist in designing poster and wiki. She likes watching anime, being eager for anything unusual. She’s a patient and honest girl, mild and energetic.

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Yu Chen

Yu Chen, undergraduate student of biology in Lanzhou University, has a great enthusiasm in doing experiment. As a member of the 2015 iGEM team of Lanzhou University, he devotes himself into making parts and MFC. He likes reading, film and animation, he also likes to play table tennis with his friends in his free time.


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Mingchu Yang

My name is Mingchu Yang. In China “YANGMING” mean “famous”. And if you read my name upside down “CHUMING” mean famous too. So my dad gave me the name of a certain hope I can have a wonderful future. This time in Igem, I gain a lot. Not only increased my knowledge also strengthened the ability experiment. Above all, in this team, I got the most important thing. That is friends.

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Huzi Cheng

Huzi Cheng designed both the visual looks and logical struct of our wiki. He also in charge of building, uploading and making sure it can works well and being attractive to viewers.He designed our annual logo ,also.He joined the visualization and the fitting of our data.


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Tianyi Shao

Hello,I am Tianyi Shao majoring in Chemistry from Lanzhou University.I don’t mean to exaggerate how splendidly our project performs in that Micro-Holmes as a pollutant detector is pragmatic and useful in every corner of this contaminated world.Three other partners and I are primarily in charge of the construction and operation of the MFCs,and collecting the data for an experienced formula that has widely applicability.I went to Japan for communication with top Japanese iGEM’s undergraduates in this summer and we all enjoyed this kind of opportunities to share our precious ideas.I am looking forward to having a brainstorm with you coming from all over the world in Boston.

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Puxin Wang

My name is Puxin Wang, an undergraduate student majoring in life science. As a new student in university, I couldn’t do some professional experiment but I can help them translate the experimental report into English. I also do the presentation at the same time.


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Shuhui Xu

It’s me. I’m Shuhui Xu. Love the sunshine and sunny days,and I love the nature. I am one of the guys who operate the MFC devices. Thinking about the tiny creature which creates visual voltage, exciting isn’t it ? I always believe that microorganism has a great potential. So ,let’s make it come true, step by step.

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Jinjin Tong

Hi, I am Jinjin Tong, an optimistic, active and sunny girl. I am a junior student majoring in life science in LanZhou University. In our team, my main task is to operate the Microbial Fuel Cells(MFC), collect data, and analyze data. I've grown a lot during preparing for the iGEM. After this summer vacation, it's going to be my last year of staying in LanZhou University. I hope I will enjoy every minute of every day with my friends and partners.


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Ningning Yao

I’m Ningning Yao, major in biology from Lanzhou University Cuiying Honors College. It’s my great honor to take part in iGEM as I am enthusiasm in life science. In the past half year, I made porin, constructed k1755003, joined the communication with some universities in Japan and accomplished the previous work of interlab. I also borrowed some experimental material from Jilin University, Nanjing University and Huazhong Agricultural University. Thanks to iGEM, this half year of experience made me feel fulfillment although my work isn’t too much. Wish LZU-2015 can get a good achievement.

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Xinyu Zhou

I'm Xinyu Zhou. An outgoing boy who loves anime very much major in biology in Lanzhou University. I like iGEM 'cause it's very creative and we can introduce our new ideas in it. This year in our team I was building part with my partners, also I took part in the human practice and what's more, I'm the lecturer of LZU in the meet up with HokkaidoU, KAIT as well as TokoyoTech in Japan.


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Yalan Yang

I’m Yalan Yang, originally working at the wet lab, but now somehow working for human practice. I took part in the publicity, including organizing public activity, registering Twitter and Weibo and finally, dramatizing for a small video. That’s all my work this year.

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Ning Chai

I’m Ning Chai, a girl loves nature and is keen on plants. During the time after joining in iGEM, I spared every free time in lab. However, when I have some break time in the lab, I often listen to the pop music or read some English magazine. By the way, Durarara was watched after setting the PCR of RibB in the break time. Now, you must know some of me.


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Qiang Ao

I’m Yalan Yang, originally working at the wet lab, but now somehow working for human practice. I took part in the publicity, including organizing public activity, registering Twitter and Weibo and finally, dramatizing for a small video. That’s all my work this year.

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Lizhe An

Prof. Lizhe An, professor of Life Science College, Vice president of Lanzhou University, went to Chinese Academy of Sciences and Bar-Ilan University to gain his postdoctor. Now his main research direction is botany ecology.

 

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Huyuan Feng

Vice dean Prof Huyuan Feng：Prof. Huyuan Feng, Doctor of philosophy graduated in Lanzhou University, as the Vice Dean of School of Life Sciences, Lanzhou University, China will lead this team. His present research subject is microbio strains living on Dunhuang Mogao Grotto and he devotes all his enthusiam to the study of protection of the historic treasure of the world - Dunhuang Frescoes. He is the ruler of us for the reasons that he is not only a professional expert of our topic but also an intelligent guide of all team.

Jinbo Yang

Prof. Jinbo Yang, Doctor of philosophy graduated in Shanghai Research Institute of Biochemistry is also a member of Cuiying Scholar. He once worked as Postdoctoral Research Fellow, Research Associate Staff, Adjunct Staff and finally became a Consultant Staff in Lerner Research Institute, The Cleveland Clinic Foundation since 2001. He is an expert of tumor treatment and synthetic biology, and in 2013 he was the adviser of LZU-China in iGEM contest.

 

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Xiangkai Li

Prof. Xiangkai Li: Prof. Xiangka Li, PhD of microbiology in the University of Oklahoma, is also a member of Cuiying Scholar. He worked as a postdoctor fellow in Baylor College of Medicine in America when finishing his PhD. And after that, he has been a professor in microbiology and doctoral advisor in Lanzhou University till now.

Qiang Qiu

Prof. Qiang Qiu, born in may 1982，Weinan ShannXi, he gained Bachelor Degree and Doctor Degree in Life Science College of Lanzhou University in 2004 and 2011. Up to now, he has worked in State Key Laboratory of Grassland Agro-Ecosystem, College of Life Science.

 

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Yongxing He

Prof. Yongxing He, bachelor of life science and doctor of molecular and biochemistry in University of Science and Technology of China, went to university of California ,Berkeley in 2011 for his postdoctor fellow in biophysics. When finishing postdoctor, he has been a professor in Lanzhou University till now.

Zhenmin Ling

Prof. Qiang Qiu, born in may 1982，Weinan ShannXi, he gained Bachelor Degree and Doctor Degree in Life Science College of Lanzhou University in 2004 and 2011. Up to now, he has worked in State Key Laboratory of Grassland Agro-Ecosystem, College of Life Science.

 

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ACKNOWLEDGEMENTS

This project is supported by School of Life Science and Cuiying Honors College, Lanzhou University, MOST international cooperation funds, 2014DFA91340, Gansu provincial international cooperation funds, 1304WCGA176, Majorbio Cooperation and Takara China.

Lanzhou University

Lanzhou University has been the best university in the northwestern region of China, ranking as one of the top 30 among over 1,500 universities across China. It is one of the key universities administered directly by the Ministry of Education.

School of Life Sciences

The School of Life Sciences at Lanzhou University has a long history. It originated as the Department of Botany and the Department of Zoology of National Lanzhou University, which were founded in 1946 as the first departments of life sciences in western China. After several generations' efforts in over sixty years, the School of Life Science is in a good development trend in many aspects, such as faculty and staff, discipline construction, teaching and researching, and local service. Basing its scientific research on the cold and arid environment in western China, the faculty has made great achievements. The School of Life Sciences is active in international collaboration in order to create an open and supportive academic atmosphere, extend academic horizons, and improve the faculty and students' ability in research and creativity.

Cuiying Honors College

Cuiying Honors College (CHC) was founded in August 2010 within Lanzhou University as part of China's Top-Notch Undergraduate Training Program. The Ministry of Education and the Ministry of Finance are investing in this new pilot scheme in order to develop future leaders with exceptional talent in the core disciplines. Every year this program only admits the best 1000 students from the 6 million new undergraduates nationwide. Lanzhou University has been selected as one of 19 institutions implementing this national program.