Difference between revisions of "Team:NTU-LIHPAO-Taiwan/Collaborations"

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Revision as of 16:29, 16 September 2015

• Home
• Team
  • Team
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• Project
  • Description
  • Design
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  • Modeling
  • Protocols
• Parts
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  • Basic Parts
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  • Part Collection
• Notebook
• Safety
• Society
  • Human Practices
  • Collaborations
  • Entrepreneurship

• Protocols
  • Preparation of Competent Cells (E.coli)
  • DNA Dissolution
  • Agarose Gel Electrophoresis
  • Gel Extraction
  • DNA Ligation
  • Plasmid DNA Extraction Using Alkaline Lysis Method (E.coli)
  • Transformation (E.coli)
  • Protein Extraction
  • SDS-PAGE
  • Western Blot
  • Immunostaining

Preparation of Competent Cells (E.coli)

Materials and Reagents :

1. Escherichia coli DH5α
2. LB broth
3. TB buffer
4. DMSO
5. Liquid nitrogen

Escherichia coli DH5α

Maintained by the iGEM team NTU-LIHPAO-Taiwan    ©2015 NTU-LIHPAO-Taiwan