Difference between revisions of "Team:Paris Saclay/Notebook/July/1"

(→‎Competente cells preparation)
(→‎Preparation LB Plate)
Line 31: Line 31:
 
Aliquot the solution in chilled 1.5mL microcentrifuge tubes (500uL/tube) and frozen and store at -70°C
 
Aliquot the solution in chilled 1.5mL microcentrifuge tubes (500uL/tube) and frozen and store at -70°C
  
−
===Preparation LB Plate===
+
===Preparation Petri dishes with LB===
 
''by Seong Koo and Pauline''
 
''by Seong Koo and Pauline''
  
−
22 Plates LB + Cm (20uL/mL)
+
22 Petri dishes with LB + Cm (20uL/mL)
−
12 Plates LB + Amp (100uL/mL)
+
12 Petri dishes with LB + Amp (100uL/mL)
  
  

Revision as of 17:34, 16 September 2015

July 1

Lab Work

Preparation

by Johan

Plasmid Rehydratation :

by Johan Measure fluorescence : I13504 J23117 J23106 J23101

For our project : R0051 J13602 J13522 C0040

Preparation of liquid culture of DH5α with K115017

K115017 5mL LB + 5uL Cm (20mg/mL)

Competente cells preparation

by Seong Koo, Pauline, Johan

Preparation competente cells DH5α with CaCl2 cf:protocol

15mL competente cells Aliquot the solution in chilled 1.5mL microcentrifuge tubes (500uL/tube) and frozen and store at -70°C

Preparation Petri dishes with LB

by Seong Koo and Pauline

22 Petri dishes with LB + Cm (20uL/mL) 12 Petri dishes with LB + Amp (100uL/mL)


Members present:

  • Instructors : Alice.
  • Students : Johan, Seong Koo, Pauline.

Back to the calendar

Contact
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France Copyright © 2015 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.