Latest revision as of 17:38, 16 September 2015

July 1

by Johan

by Johan Measure fluorescence : I13504 J23117 J23106 J23101

For our project : R0051 J13602 J13522 C0040

K115017 5mL LB + 5uL Cm (20mg/mL)

by Seong Koo, Pauline, Johan

Preparation competent cells DH5α with CaCl2 cf:protocol

15mL competente cells Aliquot the solution in chilled 1.5mL microcentrifuge tubes (500µL/tube) and frozen and store at -70°C

by Seong Koo and Pauline

22 Petri dishes with LB + Cm (20µL/mL) 12 Petri dishes with LB + Amp (100µL/mL)

Members present:

@@ Line 1: / Line 1: @@
 {{Team:Paris_Saclay/notebook_header}}
+=July 1=
 ==Lab Work==
 ====Preparation====
@@ Line 18: / Line 19: @@
 C0040
-===Preparation of liquid culture of DH5a with K115017===
+===Preparation of liquid culture of DH5α with K115017===
 K115017
 mL LB + 5uL Cm (20mg/mL)
 ===Competente cells preparation===
+''by Seong Koo, Pauline, Johan''
-''By Seong Koo, Pauline, Johan''
+Preparation competent cells DH5α with CaCl2 cf:protocol
-Preparation competente cells DH5a with CaCl2 cf:protocol
 mL competente cells
-Aliquot the solution in chilled 1.5mL microcentrifuge tubes (500uL/tube) and frozen and store at -70°C
+Aliquot the solution in chilled 1.5mL microcentrifuge tubes (500µL/tube) and frozen and store at -70°C
-===Preparation LB Plate===
+===Preparation Petri dishes with LB===
-''by SeongKoo and Pauline''
+''by Seong Koo and Pauline''
-Plates LB + Cm (20uL/mL)
+Petri dishes with LB + Cm (20µL/mL)
-Plates LB + Amp (100uL/mL)
+Petri dishes with LB + Amp (100µL/mL)