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<title>University of Pennsylvania iGEM</title>
 
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<p style="font-size:150%"> Communication between bacterial cells is essential for coordinating population-level activity. In a process known as quorum sensing, bacterial species produce a class of chemical signaling molecules, termed auto-inducers, in order to enable large groups of bacteria to synchronize gene expression paradigms. However, since chemical signals must diffuse between sender and receiver cells for successful communication to occur, communication is restrictedto cells that share the same environment and compatible parts.The 2015 Penn iGEM team has worked towards expanding cell-to-cell communication to include a transfer of information between isolated cells via light. The genetic circuits designed closely resemble an electrical system known as an optocoupler which allows for electrical signals to be transferred between two circuits operating at incompatible voltages by designing a light sender and receiver system (Figure 1). In a similar approach, we engineered a separate "sender" cell that generates light via bio luminescence and "receiver" cells that express photoreceptors to enable light-dependent physiological responses. The team also further illuminated potential applications for this alternative form of light-based communication.
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<h1>OUR PROJECT</h1>
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      <p> Communication between cells is instrumental in coordinating population-level activity. In a process known as "quorum sensing," bacteria both secrete and sense autoinducer signaling molecules to enable synchronization of group gene expression paradigms. The synthetic biology community has rapidly adopted these quorum signaling pathways for use in programmed circuitry. However, chemical signals must diffuse between sender and receiver cells, limiting such communication to a common environment. In electronics, when electrical signals must be transferred between two circuits operating at incompatible voltages, electrical engineers use optocouplers, components that transfer information between isolated circuits via light. The 2015 Penn iGEM team presents a biological analog of the optocoupler, a cell-to-cell communication system in which a "sender" cell generates light via bioluminesence and a "receiver" cell expresses photoreceptors to enable light-dependent physiological responses. We show that light elicits a response in light-sensitive receivers and illuminated potential applications for this alternative form of cell communication.</p>
 
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<h1>BREAKDOWN</h1>
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<td style = "text-align: center;"><a href="https://2014.igem.org/Team:Penn/Microbio">Microbiology in AMB-1</a></td>
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<td width="33.33%"><a href="https://2015.igem.org/Team:Penn/Sender"><img id = "daicon" src="https://static.igem.org/mediawiki/2015/4/4b/Pennigem_sender_15.png" height="85%" width="85%"></a></td>
<td style = "text-align: center;"><a href="https://2014.igem.org/Team:Penn/Microbio/Synbio">Synthetic Biology in AMB-1</a></td>
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<td style = "text-align: center;"><a href="https://2014.igem.org/Team:Penn/CdTolerance">Cadmium Tolerance in E. Coli vs. AMB-1</a></td>
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<td width="33.33%"><a href="https://2015.igem.org/Team:Penn/Communication"><img id = "daicon" src="https://static.igem.org/mediawiki/2015/f/fc/Pennigem_comm_15.png" height="85%" width="85%"></a></td>
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<h1>HUMAN PRACTICES</h1>
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<td style = "width:250;"><a href="https://2014.igem.org/Team:Penn/Microbio"><img id = "menuicon" src="https://static.igem.org/mediawiki/2014/d/df/Magnetic_bacteria_graphic.png"></a></td>
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      <p> Analagous to  our project goals, the Penn iGEM 2015 team has worked to further illuminate the parallels between genetic circuits and electrical circuits with the addition of the PennTunes Toolbox. Just as there is a distinct change in response when replacing an electric circuit component, the various genetic parts included in this toolbox clearly demonstrate the divergence in expression level due to a change in genetic part (promoter, RBS, etc). As an example, we have characterized one of the inverter strains in the toolbox that exhibits the relationship in the figure to the right.
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<p>Our aim is to increase the reach of synthetic biology by providing the tools and infrastructure that will make biotechnology more accessible in educational settings. Find out more about our vision of the future for synthetic biology and biotechnology tools by clicking on the graph! </p>
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<h1>Our Team</h1>
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Latest revision as of 19:40, 16 September 2015

University of Pennsylvania iGEM

OUR PROJECT

Communication between cells is instrumental in coordinating population-level activity. In a process known as "quorum sensing," bacteria both secrete and sense autoinducer signaling molecules to enable synchronization of group gene expression paradigms. The synthetic biology community has rapidly adopted these quorum signaling pathways for use in programmed circuitry. However, chemical signals must diffuse between sender and receiver cells, limiting such communication to a common environment. In electronics, when electrical signals must be transferred between two circuits operating at incompatible voltages, electrical engineers use optocouplers, components that transfer information between isolated circuits via light. The 2015 Penn iGEM team presents a biological analog of the optocoupler, a cell-to-cell communication system in which a "sender" cell generates light via bioluminesence and a "receiver" cell expresses photoreceptors to enable light-dependent physiological responses. We show that light elicits a response in light-sensitive receivers and illuminated potential applications for this alternative form of cell communication.

BREAKDOWN



HUMAN PRACTICES

Analagous to our project goals, the Penn iGEM 2015 team has worked to further illuminate the parallels between genetic circuits and electrical circuits with the addition of the PennTunes Toolbox. Just as there is a distinct change in response when replacing an electric circuit component, the various genetic parts included in this toolbox clearly demonstrate the divergence in expression level due to a change in genetic part (promoter, RBS, etc). As an example, we have characterized one of the inverter strains in the toolbox that exhibits the relationship in the figure to the right.

Our aim is to increase the reach of synthetic biology by providing the tools and infrastructure that will make biotechnology more accessible in educational settings. Find out more about our vision of the future for synthetic biology and biotechnology tools by clicking on the graph!

Our Team