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Revision as of 21:56, 16 September 2015

Team:BNU-CHINA - 2015.igem.org

Li Yuehuan

A sophomore bioscience student. She helped design verify parts of the module.

Wu Zekai

A sophomore biology student. Designed a part of biobrick of photoinduction system. and took part in some experiments of verifing the toxicity of Bace 16.

Liu Longping

A sophomore student who has worked on how to promote the expression rate of rMpL as well as designed the toxic test on nematode

Zhao Xueting

A sophomore in mathematics. Did work of modeling section like finding out how to put equipment on farmland, writing modeling paper, doing some translation and so on.

Siren

In Greek mythology, the Sirens were dangerous yet beautiful creatures, who lured nearby sailors with their enchanting music and eat them. We call our machine Sirens as it can attract nematodes and kill them just like the Sirens did to their meals.

Cao Yufei

A junior student in Liyun College of BNU, biology major. She is responsible for the parts design for expressing lemonene in E. coli and coresponding experiments.

Wang Yingbing

A junior biotech student of BNU. Mainly did some paper work for the team, and also took part in the “bace16” group work.

Yin Yuan

A sophomore majoring in mathematics. Helped set up the model in proposing the solving ideas and searching the dada.  Yuan Qiuyue, a sophomore majoring in mathematics. Simulated the movement of nematode, and established the model of diffusion of the attraction.

Lao Kejing

A sophomore majoring in biology. Her work includes plasmid purification, SDS-PAGE and nematodes synchronization. tried to help BIT-China with extracting membrane proteins, NhaA and NhaB. Wu Zekai , a sophomore biology student. Designed a part of biobrick of photoinduction system. and took part in some experiments of verifing the toxicity of Bace 16.

Feng Xiaofei

!!!!!!!!!A sophomore student who has worked on how to promote the expression rate of rMpL as well as designed the toxic test on nematode

Lee

Junior biology student. Participated in graphic design of our team , such as website design, logo of the team and T-shirt.

Chen Zhiyao

The only freshman in our team, majoring in physics. Developed the toxic and attractant database with Peng Chengfei. Also designed the UI of the database website, and took part in HP activities too!

Cao Yalei

From school of Geography in BNU, is a member of graphic design group who designed our team logo, banner and poster. The jersey and souvenir were also her masterpieces.

Attribution

Under the supervision of Prof. Zhu Xudong, Prof. Yang Dong, and Prof. Xiang Benqiong etc, nearly all team members have taken part in the project designing process, especially those of wet lab group.Beichen Gao , Yuanyi Dai , Yufei Cao and Jia Li are the group leaders who mainly design the experimental patterns and guide the different parts of the experiments.
Cheng Li is the leader of modeling group, who mainly enrolled in designing the device 2.0. Yuan Yin , Qiuyue Yuan and Xueting Zhao also do a great many works in the modeling group, such as information searching, paper writing and equipment appilication.
Jiajun Zhang , who has led the web design group to build the wiki, together with group members Zhiyao Chen and Chengfei Peng complete the main work in database construction and maintenance.
Lu Xu( group leader), Lee and Yalei Cao really do a fantastic work in graphic and web design. They all make great efforts in designing our team logo, website, poster, souvenir, T-shirt and so on.
Meng Tang , the leader of human practice group, has organized a series of activities for Policy and Practice, like survey and publicize in some middle schools etc. Some group members as Yuanyi Dai,Jia Li , Jiajun Zhang , Beichen Gao , Zhiyao Chen , Jingyu Zhao , Xiaofei Feng also play a significant role in planning and organizing the activities . Other group members also take an active part in some other activities of human practice.
Team MemberAttributions
Cao YaleiGraphic Design(Design of logo, banner, poster, jersey and souvenir)
Cao YufeiWet lab group leader(parts design for expressing lemonene in E. coli and coresponding experiments)
Chen ZhiyaoWiki construction(database construction; UI design)
Human practice
Dai YuanyiDaily activities arrangement and personnel management
Wet lab group leader(biobricks construction, Bace16 expression and testification)
Human practice
Feng XiaofeiWet lab experiments(limonene synthesis)
Human practice
Gao BeichenTeam leader
Wet lab group leader(biobricks construction, nematocidal protein expression, rMpL's toxicity identification
Human practice
Lao KejingWet lab experiments(plasmid purification; SDS-PAGE; nematodes synchronization)
Helping Beijing Institute of Technology with extracting membrane proteins, NhaA and NhaB
LeeGraphic Design( website design, logo of the team and T-shirt)
Li ChengModeling leader(design of the device 2.0 ; parts improving)
Wiki
Graphic design
Li JiaWet lab group leader(chemical attractants' function testification, limonene and GPPs expression, C.elegans cultivation )
Human practice
Li YuehuanWet lab experiments(rMpL verification)
Liu LongpingWet lab experiments(exploraton of how to promote the expression rate of protein rMpL; design of the toxic test)
Liu XinyuWet lab experiments(limonene group and other parts' experiments)
Human practice
Ma HongfeiWet lab experiments(GC-MS of limonene )
Modeling(device 1.0 design and creation)
Peng ChengfeiWiki construction(database construction, deployment and operation, Wiki frame construction)
Tang MengHuman practice project leader(activities planning and organization)
Wet lab experiments
Wang RanTranslation
Human practice
Wang YingbingWet lab experiments(Bace16 group work)
Wu ZekaiWet lab experiments(photoinduced system design; Bace 16 functional verification)
Xiao ShushengLiterature reading
Human practice
Xu LuGraphic design leader(mainly on poster and PPT design)
Yin YuanModeling(proposing the solving ideas; searching the data)
Yuan QiuyueModeling(nematode movement simulation;model of diffusion of the attraction)
Human practice
Zhang JiajunWiki leader
Rear services
Human practice
Zhang TianqiRear services
Wet lab experiments(validating limonene's attracting effects on nematodes)
Zhao JingyuWet lab experiments(rMPL expression)
Zhao XuetingModeling(solving problems like how to put equipment on farmland; paper writing; translation)

Collaboration

Our modeling can be divided into four parts. Firstly, in order to enable our project to be applied in life, we design a device placed in soil, which can attract and kill nematodes by modified engineering bacteria inside. Secondly, assuming there is a farmland, we take advantage of nematodes’ movement analogue simulation to find the best position where the device should be placed and the best concentration of attracting substance. Thirdly, according to this concentration, we obtain the best size of the device by calculating. At last, we establish a database to enlarge applied range of our method to kill other pests. We would appreciate that new synthetic biological and environment-friendly methods can be shared and improved with the science researchers all over the world.

Acknowledgement

Thanks:

Prof. Zhu Xudong, Prof. Yang Dong and Prof. Xiang Benqiong

Offered us much precious advice on the project.

Hao Xiaoran, Chi Xiaodong, Huo Liang, Ai Ying and Wang Xuan

Offered us advice on our experiments and helped us solve problems.

The College of Chemistry, Beijing Normal University

Helped us do GC-MS.

Prof. Yang Chonglin of Chinese Academy of Sciences

Offered us C.elegens and taught us how to cultivate them.

Prof. Li Hongmei of Department of Plant Pathology,Nanjing Agricultural University

Helped us to do Species identification of nematodes.

BIT-China & ZJU-China & FAFU-CHINA

Offered us some helpful advice and we also had a collaboration together.

Agricultural Service Center of Ceyu, Hebei Province

Helped us learn more information about the damage caused by local nematodes and villager’s attitude towards agricultural insecticide.

Shang Zhong National Middle School of Guizhou Province

Supported us in our voluntary education.

National Chiao Tung University &Peking University

Provided us opportunities to communicate with other teams.

Feng Xiaofei and her family in Hebei Province

Helped us get soybeans samples and did a survey in the locality.

Prof. Wang Yingdian

Discussed the bright future of our project with us.

Sponsors

Beijing Normal University (BNU), is a public research university located in Beijing with strong emphasis on basic disciplines of humanities and sciences. It is one of the oldest and most prestigious universities in China. Our school supported us to our iGEM Journey and provided us adequate resources to complete the competition. Students of different colleges in our school took part in our team, and all these colleges supported us very much.