Difference between revisions of "Template:IONIS Paris/Notebook/11-09-15"
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Revision as of 22:44, 16 September 2015
Digestion
| Tube | VVD YC | VVD YN |
|---|---|---|
| H2O MQ | 1 µL | 1 µL |
| Buffer 2.1 | 2 µL | 2 µL |
| DNA | 15 µL | 15 µL |
| Enzyme 1 | EcoRI 1 µL | XbaI 1 µL |
| Enzyme 2 | SpeI 1 µL | PstI 1 µL |
37°C, 1h heat kill: 65°C, 20min
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11 Sept. 15