Difference between revisions of "Team:Penn/Sender"

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     <p>The first component of our project focused on will be used to determine if the light produced by the lux box is
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     <p>An effective light-based communication system rests on the bioluminesence generated by the “sender cell.”  In order to design a well-functioning system, the Penn 2015 iGEM team worked to optimize the light output of various E.coli “sender cells” transformed with the lux operon (BBa_K325909). </p>
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sufficient to activate the light-activated transcription factor. This experiment requires a new
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component to the aforementioned genetic circuit in pDawn. The new component is initiated by a  
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constitutive promoter (BBa_J23100) and followed by an RBS + lux box. Expression of the genes
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in the lux box is responsible for producing luminescence. The box contains the following genes
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in order: LuxC, D, A, B, E and G. LuxA and B are responsible for encoding two subunits of
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bacterial luciferase. The genes LuxC, D, and E code for the substrate for the light emitting
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reaction, tetradecanal. The function of the luxG gene is yet to be fully elucidated; however,
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inclusion of the gene is known to increase light output. The circuit is completed with a stop
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codon and a terminator sequence. </p>
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<div><a href="https://2015.igem.org/Team:Penn/Educational_Toolbox"><img src="https://static.igem.org/mediawiki/2015/6/6b/Luminesence_Graph1.png" alt></a></div>
 
<div><a href="https://2015.igem.org/Team:Penn/Educational_Toolbox"><img src="https://static.igem.org/mediawiki/2015/6/6b/Luminesence_Graph1.png" alt></a></div>

Revision as of 23:24, 16 September 2015

PENN iGEM 2015



SENDER

An effective light-based communication system rests on the bioluminesence generated by the “sender cell.” In order to design a well-functioning system, the Penn 2015 iGEM team worked to optimize the light output of various E.coli “sender cells” transformed with the lux operon (BBa_K325909).

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