Difference between revisions of "Team:Chalmers-Gothenburg/BioBricks"

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<h1>BioBricks</h1>
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<h2>1. BBa_K1321000: pTEF1-pSUC2</h2>
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<p>The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low glucose concentrations. </p>
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<h2>2. BBa_K1321001: STE2MAM2</h2>
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<p>Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway. </p>
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<h2>3. BBa_K1321002: RecA</h2>
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<p>Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms.
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Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair
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</p>
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<h2>4. BBa_K1321004: pTPI1</h2>
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<p>promoter to TPI1. </p>
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<h2>5. BBa_K1321005: Ssb</h2>
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<p>From Deinococcus Radiodurans
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Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair
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</p>

Revision as of 23:41, 16 September 2015



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BioBricks

1. BBa_K1321000: pTEF1-pSUC2

The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low glucose concentrations.

2. BBa_K1321001: STE2MAM2

Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway.

3. BBa_K1321002: RecA

Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair

4. BBa_K1321004: pTPI1

promoter to TPI1.

5. BBa_K1321005: Ssb

From Deinococcus Radiodurans Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair