Difference between revisions of "Team:Penn/Sender"

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   <p style="font-size:30px" align="center"><br>IS THE LIGHT PRODUCED BY THE SENDER CELL SUFFICIENT TO ACTIVATE THE RECEIVER CELL?</span>
 
   <p style="font-size:30px" align="center"><br>IS THE LIGHT PRODUCED BY THE SENDER CELL SUFFICIENT TO ACTIVATE THE RECEIVER CELL?</span>
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<p>Introduction</p><br>
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<p class="margin-top-10"><br><b>INTRODUCTION</b> </p>
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    <p>An effective light-based communication system rests on the bioluminesence generated by the “sender cell.”  In order to design a well-functioning system, the Penn 2015 iGEM team worked to optimize the light output of various E.coli “sender cell” populations transformed with the lux operon (BBa_K325909). </p>
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     <p class="margin-top-10">Lux operon expression is responsible for bioluminescence. The operon is initiated by a constitutive promoter (BBa_J23100) followed by an RBS + lux box. The box contains the following: LuxC, D, A, B, E and G. LuxA and B encode two subunits of bacterial luciferase. The genes LuxC, D, and E drive expression of the substrate for the light-emitting reaction, tetradecanal. The function of the luxG gene is yet to be fully elucidated; however, inclusion of the gene is known to increase light output (CITATION). The circuit is completed with a stop codon and a terminator sequence.</p>
 
     <p class="margin-top-10">Lux operon expression is responsible for bioluminescence. The operon is initiated by a constitutive promoter (BBa_J23100) followed by an RBS + lux box. The box contains the following: LuxC, D, A, B, E and G. LuxA and B encode two subunits of bacterial luciferase. The genes LuxC, D, and E drive expression of the substrate for the light-emitting reaction, tetradecanal. The function of the luxG gene is yet to be fully elucidated; however, inclusion of the gene is known to increase light output (CITATION). The circuit is completed with a stop codon and a terminator sequence.</p>

Revision as of 23:50, 16 September 2015

University of Pennsylvania iGEM

PENN iGEM 2015



SENDER


IS THE LIGHT PRODUCED BY THE SENDER CELL SUFFICIENT TO ACTIVATE THE RECEIVER CELL?


INTRODUCTION

Lux operon expression is responsible for bioluminescence. The operon is initiated by a constitutive promoter (BBa_J23100) followed by an RBS + lux box. The box contains the following: LuxC, D, A, B, E and G. LuxA and B encode two subunits of bacterial luciferase. The genes LuxC, D, and E drive expression of the substrate for the light-emitting reaction, tetradecanal. The function of the luxG gene is yet to be fully elucidated; however, inclusion of the gene is known to increase light output (CITATION). The circuit is completed with a stop codon and a terminator sequence.