Difference between revisions of "Team:Penn/Sender"

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<p class="margin-top-10"><br><b>INTRODUCTION</b> </p>
 
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An effective light-based communication system rests on the bioluminesence generated by the “sender cell.”  In order to design a well-functioning system, the Penn 2015 iGEM team worked to optimize the light output of various E.coli “sender cells” transformed with the lux operon (BBa_K325909).
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     <p class="margin-top-10">Lux operon expression is responsible for bioluminescence. The operon is initiated by a constitutive promoter (BBa_J23100) followed by an RBS + lux box. The box contains the following: LuxC, D, A, B, E and G. LuxA and B encode two subunits of bacterial luciferase. The genes LuxC, D, and E drive expression of the substrate for the light-emitting reaction, tetradecanal. The function of the luxG gene is yet to be fully elucidated; however, inclusion of the gene is known to increase light output (CITATION). The circuit is completed with a stop codon and a terminator sequence.</p>
 
     <p class="margin-top-10">Lux operon expression is responsible for bioluminescence. The operon is initiated by a constitutive promoter (BBa_J23100) followed by an RBS + lux box. The box contains the following: LuxC, D, A, B, E and G. LuxA and B encode two subunits of bacterial luciferase. The genes LuxC, D, and E drive expression of the substrate for the light-emitting reaction, tetradecanal. The function of the luxG gene is yet to be fully elucidated; however, inclusion of the gene is known to increase light output (CITATION). The circuit is completed with a stop codon and a terminator sequence.</p>
  
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<p class="line-break margin-top-10">Our sender cell characterization was founded on determining the photons/second trends for luminescing cell populations. This information was important in order to determine if light produced by the lux box is sufficient to activate the light-activated transcription factor of the receiver cell population. </p>
  
 
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Revision as of 23:51, 16 September 2015

University of Pennsylvania iGEM

PENN iGEM 2015



SENDER


IS THE LIGHT PRODUCED BY THE SENDER CELL SUFFICIENT TO ACTIVATE THE RECEIVER CELL?


INTRODUCTION

An effective light-based communication system rests on the bioluminesence generated by the “sender cell.” In order to design a well-functioning system, the Penn 2015 iGEM team worked to optimize the light output of various E.coli “sender cells” transformed with the lux operon (BBa_K325909).

Lux operon expression is responsible for bioluminescence. The operon is initiated by a constitutive promoter (BBa_J23100) followed by an RBS + lux box. The box contains the following: LuxC, D, A, B, E and G. LuxA and B encode two subunits of bacterial luciferase. The genes LuxC, D, and E drive expression of the substrate for the light-emitting reaction, tetradecanal. The function of the luxG gene is yet to be fully elucidated; however, inclusion of the gene is known to increase light output (CITATION). The circuit is completed with a stop codon and a terminator sequence.

Our sender cell characterization was founded on determining the photons/second trends for luminescing cell populations. This information was important in order to determine if light produced by the lux box is sufficient to activate the light-activated transcription factor of the receiver cell population.